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Citations for Promega :
1101 - 1150 of 1480 citations for Recombinant Human GFRA2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Protein digestion was performed overnight at 37 °C in a shaking incubator using 0.12 μg trypsin (Promega) at an enzyme to protein ratio of 1:50 (protein content was calculated using independent samples) ...
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bioRxiv - Microbiology 2021Quote: ... SDS was removed according to previous work (52) and the proteins were proteolytically digested with trypsin (Promega) and purified using OMIX C18 Mini-Bed tips (Agilent Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... IVT reactions were done with the TNT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... Proteins were digested on-filter (16 hr, 37 °C) with sequencing-grade modified trypsin (50:1, Promega). Proteolysis was terminated and peptides eluted with 20 μL of 10% trifluoroacetic acid followed by centrifugation (13,000 ×g ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were selected by detection of HiBit protein using Nano Glo HiBiT Lytic Detection System (N3030, Promega). pX330 was a kind gift from Dr ...
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bioRxiv - Plant Biology 2021Quote: ... The entire protein region of the gel was excised and subjected to in-gel trypsin (Promega V511A) digestion after reduction with DTT and alkylation with iodoacetamide ...
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bioRxiv - Physiology 2020Quote: ... proteins were digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) for 12 h at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... Protein for MUG and LUC assays was extracted on ice in 1x passive lysis buffer (PLB: Promega). MUG assays were performed by adding 40μl of protein extract to 100μl of MUG assay buffer (2mM MUG ...
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bioRxiv - Molecular Biology 2020Quote: ... protein samples were treated with Endoprotease Lys-C (Wako) and Trypsin (Trypsin Gold Mass Spec Grade; Promega). Peptide samples were desalted by OMIX C18 pipette tips (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... proteins were digested overnight with sequencing-grade porcine trypsin (1:25, w/w, Promega, Fitchburg, MA, USA). The resulting vacuum-dried peptides were resuspended in water containing 0.1% (v/v ...
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bioRxiv - Genetics 2019Quote: ... proteins were digested overnight with sequencing-grade porcine trypsin (1:25, w/w, Promega, Fitchburg, MA, USA). The resulting vacuum-dried peptides were resuspended in water containing 0.1% (v/v ...
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bioRxiv - Evolutionary Biology 2021Quote: Thirty µg of the heterologously expressed proteins were deglycosylated with endoglycosidase H following the manufacturer’s protocol (Promega GmbH ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako) and 1 μg trypsin (Promega) overnight at 37°C on a shaking incubator ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 50 ng of plasmids encoding the HiBiT-tagged proteins using FuGENE HD (Promega). Cells were trypsinized (Gibco ...
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bioRxiv - Cell Biology 2021Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (SP6 or T7, Promega). Translation products were partially purified by ammonium sulfate precipitation (Bardwell & Shah ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were alkylated and digested with endopeptidase Lys-C (Wako) for 3 hours and trypsin (Promega) on beads overnight at 37°C.
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bioRxiv - Immunology 2021Quote: ... and used to generate 35S-methionine labelled AIM2 protein by in vitro transcription and translation (IVTT) (Promega). Immunoprecipitations (IP ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was produced as described before.25 Sf21 cells were transfected with bacmids using Fugene6 (Promega #E2693). V0 virus containing media was harvested after 7 days ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were in-gel digested by addition of 200 µL of 2 ng/µl trypsin (Promega, V511A) in resuspension buffer (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... Trapped proteins were washed four times with methanol TEAB buffer followed by overnight Trypsin (1 μg, Promega) digestion at 37 °C ...
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bioRxiv - Immunology 2022Quote: The protein sequences of the selected seven Listeria genes were cloned into a pGEM4z-plasmid vector (Promega) containing a T7 promoter ...
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bioRxiv - Systems Biology 2022Quote: ... Proteins were digested by incubation with trypsin (1/100, w/w, Sequencing Grade Modified Trypsin, Porcine; Promega) for at least 9 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and proteins were digested overnight with a solution of sequencing-grade trypsin (Promega, San Luis Obispo, CA) in 50 mM TEAB at a 1:50 (wt:wt ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of GST-tagged effector proteins and 2 μl of FluoroTect™ GreenLys tRNA (Promega, USA) were gently mixed with High-Yield Wheat Germ Master Mix ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were digested overnight at room temperature with 0.4 µg of sequencing grade modified trypsin (Promega, #V5111) in 40 µl Hepes/NaOH ...
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bioRxiv - Cell Biology 2022Quote: ... [35S]-methionine-labelled proteins were transcribed and translated using a TnT™ coupled transcription–translation kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... and proteins were digested overnight with a solution of sequencing-grade trypsin (Promega, San Luis Obispo, CA) in 50 mM TEAB at a 1:50 (wt:wt ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were digested overnight at room temperature with 0.4 μg of sequencing grade modified trypsin (Promega, #V5111) in 40 μl Hepes/NaOH ...
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bioRxiv - Microbiology 2024Quote: ... Reduced and alkylated proteins were in-gel digested with 100 ng of trypsin (modified sequencing grade, Promega) overnight at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was carefully collected and loaded onto 30 µl MagneHis™ beads (Protein Purification System, Promega) and incubated for 1 hour ...
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bioRxiv - Systems Biology 2024Quote: ... 75 µl of digestion buffer containing 0.8 µg Trypsin Gold (enzyme to protein ratio 1:25, Promega) in 100 mM triethylammonium bicarbonate (TEAB ...
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bioRxiv - Immunology 2024Quote: ... FC tags on the S and RBD proteins were removed through treatment with Factor Xa Proteinase (Promega).
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bioRxiv - Cell Biology 2024Quote: ... proteins were digested by incubation with sequencing-grade modified trypsin (1/100, w/w; Promega, Madison, Wisconsin) for 12 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... alkylated with 10 mM iodoacetamide and subject to sequential protein digestion with Lys-C and trypsin (Promega). Digestion was halted with formic acid ...
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bioRxiv - Microbiology 2024Quote: ... mNeonGreen and Halotag fusion proteins were detected using polyclonal anti-mNeonGreen (NC, unpublished) and anti-Halotag (Promega) antibodies diluted at 1:10,000 and 1:2,000 respectively ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 μg of mitochondrial protein in triplicate was incubated in GTPase Buffer (Promega #V7681; Madison, WI, USA) with 10 μM GTP and 1 mM DTT ...
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bioRxiv - Microbiology 2023Quote: ... Protein bands were detected using a G:BOX (Syngene) after treating membranes with ECL Western Blotting Substrates (Promega). Densitometry was performed using ImageJ software.
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bioRxiv - Immunology 2023Quote: ... VP1-3 proteins were then bound to the S-Trap column and digested with trypsin (Promega Corporation) for 2 hrs at 47°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at room temperature with 0.4 µg of sequencing grade modified trypsin (Promega, #V5111) in 40 µl Hepes/NaOH ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at room temperature with 0.4 µg of sequencing grade modified trypsin (Promega, #V5111) in 40 µl Hepes/NaOH ...
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bioRxiv - Biochemistry 2023Quote: GTPase activity of the purified protein was measure using the Promega GTPase-Glo™ kit (Promega, V7681), as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) for 12h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) for 12 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Physiology 2023Quote: ... Thirty μg of total protein extract was isolated from the VSMCs using 1X passive lysis buffer (Promega) and was mixed with radio-labeled oligo for binding ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and protein pellet was resuspended in 50 mM ammonium bicarbonate and digested with sequencing grade trypsin (Promega) overnight at 37 degrees C ...
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bioRxiv - Systems Biology 2023Quote: ... The biotinylated proteins bound to the beads were digested with sequencing-grade trypsin (Promega, Madison, WI, USA) in 100 mM ammonium bicarbonate (pH 8 ...
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bioRxiv - Systems Biology 2023Quote: ... then proteins were digested with 1 μg of mass spectrometry grade Trypsin/Lys-C enzyme mix (Promega) in 50 μL of digestion buffer (100 mM ABC ...