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Citations for Promega :
1101 - 1150 of 5039 citations for 6H Imidazo 4 5 1 de acridin 6 one 5 3 diethylamino propyl amino 8 hydroxy since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... 0.4 µg envelope vector (pMD2.G) and 1.6 µg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...
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bioRxiv - Bioengineering 2023Quote: ... PB-CAG-H2B-mCherry-IPuroR and pCMV-hyPBase were co-transfected into KhES-1 using Fugene 6 (Promega, E269A) transfection reagent following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected 2 days after plating with 1 μg of total DNA with FuGENE 6 transfection reagent (E2691; Promega) at a ratio 1:3.
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: HEK293 IFITM3−/− cells were plated in a 6-well dish for 24 hours prior to transfection of IFITM3-pcDNA3.1 plasmid DNA using FuGene 6 reagent (Promega) at a ratio of 3:1 ...
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bioRxiv - Microbiology 2021Quote: ... or 3) Pepsin (Promega). 1 ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µL RNasin (Promega) and 100 µL lysis buffer for a total volume of 300 µL for IP by rotation for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: The sequence encoding RARα LBD (amino acids 176-421) was cloned into pBIND vector (Promega E245A) to generate pBIND-RARα LBD ...
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bioRxiv - Microbiology 2023Quote: ... the cells were co-transfected using Fugene HD transfecting reagent at a 1:3 ratio (Promega) along with pRL-TK Renilla (Renilla Luciferase ...
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bioRxiv - Plant Biology 2023Quote: ... LUC images were taken 2-3 days after infiltration by applying 1 mM Beetle luciferin (Promega) on adaxial leaf surface.
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bioRxiv - Cell Biology 2019Quote: ... The reaction was diluted 1:4 with 50 mM ammonium bicarbonate and incubated with 1 µg of Trypsin Gold (Promega) at 37°C for more than 18 h before desalting ...
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bioRxiv - Cancer Biology 2021Quote: ... 50μl of either caspase-8 reagent or caspase-9 (Promega) reagent was then added with MG132 proteosome inhibitor ...
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bioRxiv - Biochemistry 2022Quote: ... 50 mM Tris pH 8 overnight at 37 °C (Promega) at an estimated protein:enzyme ratio 1:100 ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... cDNA samples were then diluted to 2.5-5 ng/µl and measured in a qPCR reaction with the GoTaq® qPCR Master Mix (Promega, Walldorf, Germany). All reactions were performed by a 2-step PCR in triplicates followed by melting curve analysis on a ViiA7 real-time PCR cycler (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... reverse transcription assays were performed according to the manufacturer’s instructions with 2,5 μg of total RNA in the presence of 5 μM of oligo(dT) primer (Table S3) and 25 ng/μl of Random Primers (Promega Cat#C118A) using the SuperScript™ IV Reverse Transcriptase (Invitrogen Cat#18090010) ...
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bioRxiv - Cancer Biology 2019Quote: ... with 200 ng of miR-211 overexpression construct or empty vector along with 5 ng of pRL-CMV plasmid vector encoding Renilla luciferase gene using FuGENE transfection reagent (Promega, Madison, WI). After 48 h incubation ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were first digested for 5 h at 37 °C with 500 ng rLys-C Mass Spec Grade (Promega, Madison, WI, USA) before being diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration below 2 M urea ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µl of cDNA per sample were used and qRT-PCR was performed using the GoTaq® qPCR Master Mix (Promega, A6001) on a LightCycler 96 (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombination was verified by PCR with primers listed in Supplementary table 5 and genomic DNA isolated with the ReliaPrep™ gDNA Tissue Miniprep System (Promega, A2052) and peqGOLD Tissue DNA Mini Kit (Peqlab ...
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bioRxiv - Neuroscience 2020Quote: ... samples were digested with 50 μl of 50 mM ammonium bicarbonate solution containing 5 ng/μl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... reduced with 5 mM of DTT and alkylated with 11 mM iodoacetamide which was followed by in-gel digestion with trypsin (Promega, Madison, WI), chymotrypsin (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... Each sample was then diluted 10-fold with 50 mM ammonium bicarbonate for digestion with 0.375 µg (for 15 µg protein samples) or 0.125 µg (for 5 µg protein samples) sequencing grade modified porcine trypsin (Promega, Madison, WI, USA). The samples were incubated at 45°C for 1 hour in a chilled microwave (CEM ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM ABC was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Microbiology 2021Quote: ... the samples were incubated for 5 min at room temperature with equivalent volumes of Bright-GloTM firefly luciferase substrate (Promega, Madison, WI), and the luciferase signal was measured using a TopCount NXT plate reader (PerkinElmer Life Sciences ...
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bioRxiv - Molecular Biology 2021Quote: ... cooled on ice to room temperature for 5 min and digested overnight at 37 °C with 0.5 μg of sequencing-grade trypsin (Promega Cat. No. V5111). Peptide mixtures were acidified to pH 3 with 1.5 µl 5% FA ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was determined for the serially diluted cells 5 days after seeding with the Cell Titer Glo assay (Promega, cat# G7573) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized via reverse-transcription of 2-5 μg of RNA using M-MLV Reverse Transcriptase (200 U, Promega, Wisconsin, USA), random primers (10 ng/μl ...
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bioRxiv - Cancer Biology 2022Quote: Cells were seeded on 24-well cell culture plates and transfected in triplicates with 20 ng of firefly luciferase reporter pGL3 β-catenin-activated reporter (pBAR) (19099249) and 5 ng of renilla luciferase reporter pGL4.73 hRLuc/SV40 (RLuc; Promega, WI, USA) and a membrane GFP (75 ng as control for transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... SARS-CoV-2 5’-UTRC75G and SARS-CoV-2 5’-UTRC75G + G61C gBlocks™ DNA fragments (Integrated DNA Technologies) were cloned immediately upstream of fLuc into pGL3-fLuc plasmids (Promega, #E1751). Cells were transfected as per manufacturer protocol (Polyplus ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM TEAB was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... a mixture of 20 ng of CD44-smBiT and 5 ng of LgBiT-MSN(T558D) plasmids was added to 75 ng of Fugene HD (Promega, Cat#2312) transfection reagent diluted in DMEM ...
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bioRxiv - Biophysics 2023Quote: HeLa cells expressing each Halo-tagged chromatin remodeler were labeled with 5 nM Janelia Fluor® 549 (JF549) Halo-tag® ligand (GA1110, Promega) for 15 min at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... and the HBB 5′ UTR (ENST00000335295.4) commercially generated (IDT) sequences were each inserted into the pNL1.1 NanoLuc luciferase reporter plasmid (Promega, GenBank Accession Number JQ437370) downstream of a T7 promoter using overlap-extension PCR with Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Immunology 2024Quote: ... AP20187 dimerizer was added in cell culture media to a final concentration of 500 nM for 5 hours before taking the supernatants for LDH assay (G1780, Promega, Madison, WI). Cell death was measured on a scale with the media without cells set to 0% and cells lysed for 1 hour in 1% Triton X-100 set to 100%.
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bioRxiv - Cell Biology 2024Quote: ... 50,000 cells were seeded on 3.5 cm imaging dishes and grown to 40% confluency prior to transfection with the indicated plasmids using FuGENE HD (Promega, Madison, WI). In some cases ...
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bioRxiv - Biochemistry 2023Quote: ... All samples were then diluted 8-fold with 100 mM NH4HCO3 and 1 mM CaCl2 and digested with sequencing-grade modified porcine trypsin (Promega, WI, USA) provided at a 1 ...
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bioRxiv - Biochemistry 2021Quote: ... treated with 3 mM dithiothreitol (DTT) (molecular grade, Cat. 3483-12-3, Promega), and incubated for 30 min at 37°C in 5% CO2 in serum-free RPMI media ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with Celltiter 96 aqueous one solution reagent (Promega) for additional 2 h and the cell viability was determined by the absorbance of media at 490 nm as described in the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... One was incubated with 50 mg/mL of RNase A (Promega) at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega) was performed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... was added to one plate and CellTiter-Glo 2.0 (Promega G9242) was added to another plate ...
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bioRxiv - Bioengineering 2019Quote: ... cell proliferation was determined with Cell Titer Aqueous One Solution (Promega). Results were expressed as the percentage of cell viability compared to untreated cells.
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bioRxiv - Bioengineering 2019Quote: ... cell viability was determined with Cell Titer Aqueous One Solution (Promega). Results were expressed as the percentage of cell viability compared to cells treated with actinomycin D only.
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bioRxiv - Immunology 2021Quote: ... cells were lysed using ONE-Glo EX Luciferase Assay System (Promega) according to the manufacturer’s recommendations and assayed for luciferase activity using the EnSightMultimode Plate Reader (PerkinElmer) ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μL of ONE-gloTM EX luciferase assay reagent (Promega, SG) was added to each well and the luminescence values were read on the Tecan Spark 100M ...
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bioRxiv - Plant Biology 2021Quote: ... the tobacco leaves were sprayed with one millimolar luciferin (Promega, USA) on the blade back and kept in dark at least for six minutes ...
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bioRxiv - Biochemistry 2021Quote: ... medium was removed and 80 μL One-Glo-EX substrate (Promega) was added to the cells and incubated in the dark for 10 min prior reading on a Varioskan LUX plate reader (ThermoFisher).
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription was performed for one hour using random priming (Promega). qPCR reactions (0.5 μl cDNA ...
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bioRxiv - Cell Biology 2022Quote: ... 50 μL of ONE-Glo™ Luciferase Assay System substrate (Promega) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... using the CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega), as described before (20) ...
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bioRxiv - Immunology 2022Quote: ... Luciferase reagent was prepared (ONE-Glo Luciferase Assay System; E6110, Promega) per recommended protocol ...