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Citations for Promega :
1101 - 1150 of 4181 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: The assay was adapted from 15 where Hyp1-Nluc schizonts at 1% hematocrit and 1-2% parasitaemia were lysed in 1x NanoGlo buffer (Promega, USA) within a 96-well flat-bottom plate and parasite lysates were added to compounds and incubated for 10 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... washed with PBS and inflammasome activation was assessed using qPCR or inflammasome caspase-1 activity was measured using Caspase-Glo® 1 inflammasome assay kit (G9951; Promega) according to manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... The membrane was then washed thrice with 1X PBST and incubated for 1 hr with HRP conjugated secondary antibody (1:5000; Promega #w4018). After washing with PBST the membrane was developed using the enhanced chemiluminescence reagent (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesised using 1 µg RNA and 500 ng µl-1 Oligo(dT)15 primer (Promega, Southampton, UK), denatured at 65°C for 5 min ...
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bioRxiv - Physiology 2024Quote: ... further steps were performed according to the manufacturer’s protocol using proteases trypsin and Lys-C (2 h at 47° C, 1:15 and 1:30, respectively, Promega, USA). Peptides (20 μg ...
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bioRxiv - Cell Biology 2024Quote: ... The HMEC cell lines stably expressing doxycycline-inducible Rb variants were generated by transfecting cells with 1 µg of doxycycline- inducible plasmid and 1 µg of PiggyBac Transposase plasmid using the FuGene HD reagent (Promega E2311). Zeocin (300 µg/ml ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then fixed in 1% paraformaldehyde/PBS for 1 minute and washed with PBS and with 2X SSC (Promega, V4261), followed by Incubation in 50%formamide/2X SSC (pH7-7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 ml ice-cold wash buffer 1 (1x PBS with 2 % BSA, 1 mM DTT and 0.5 U/µl RNasin® Plus Ribonuclease Inhibitor - Promega #N2615) was added and cells were spun at 500 g for 3 min at 4 C ...
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bioRxiv - Physiology 2024Quote: ... and hydrolyzed into the S-Trap column using trypsin and Lys-C (2 h at 47 °C, 1:15 and 1:30, respectively, all Promega, USA) in 40 μl 50 mM triethylammonium bicarbonate according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were washed with Wash Buffer three more times and nuclei pellets were resuspended in Diluted Nuclei Buffer (1x Nuclei Buffer, 10x genomicsTM, supplemented with 1 mM DTT and 1 U/uL RnaseIn, Promega©) for counting and scMulti library preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... 2mM Mg(OAc)2 and 10μM of the amino acid mixture (Promega). Reactions were incubated with 5μM of the indicated nsp1 protein for 30 min on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μg of RNase A (Promega), and 40 units of RNase ONE (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... 4 µl RT buffer 5x (Promega), 2.5 μl dNTPs 2.5 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µg Trypsin/LysC mix (Promega) were added and the sample incubated for 4 h at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µg Trypsin/LysC mix (Promega) were added and the sample incubated for 4 h at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The following day activity of caspases 3 and 7 was measured using the Caspase-Glo® 3/7 Assay (Promega), according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2021Quote: ... Apoptosis was quantified by measuring caspase 3/7 activation of a luminescent signal using Caspase-Glo 3/7 assay (Promega).
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bioRxiv - Cancer Biology 2021Quote: Caspase 3/7 and 9 activities were assessed using a fluorescence-based Apo-ONE homogenous caspase 3/7 assay kit (Promega) and luminescence-based caspase-glo 9 assay system (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulting 3’UTR-Wild and 3’UTR-Mutant genes were inserted into the XbaI restriction site of pGL3-control vector (Promega) expressing firefly luciferase ...
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bioRxiv - Cancer Biology 2020Quote: ... grown for 48 h and then caspase 3/7 enzymatic activity determined using Apo-One Homogenous caspase 3/7 activity assay kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Caspase3/7 activity was measured 3 days after the treatment with a commercial available kit (Caspase-Glo 3/7 Assay, Promega).
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bioRxiv - Cell Biology 2022Quote: ... Caspase 3/7 activity was assayed according to the manufacturer’s protocol for ApoOne® Homogenous Caspase 3/7 Assay (Promega). Fluorescence was measured with Glomax Multi plate reader (Promega).
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bioRxiv - Cancer Biology 2022Quote: ... Treatments were removed 48 or 72 h later and enzymatic activities of caspase-3 and -7 were measured using Caspase-Glo 3/7 Assay (Promega) and read by a microplate reader (SpectraMax M5).
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bioRxiv - Cancer Biology 2022Quote: Caspase 3/7 activity was measured using a luminescence Caspase Glo 3/7 assay kit (G8090; Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... induction of apoptosis was determined by measurement of caspase-3/7 activity using the luminometric Caspase-Glo 3/7 assay (Promega) according to the manufacturer’s protocol and a microplate reader (Microplate Reader SH900) ...
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bioRxiv - Microbiology 2023Quote: ... Caspase 3/7 enzymatic activity in raw cell lysates was measured using a Caspase Glo 3/7 assay kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis was measured by detecting Caspase 3/7 activity after 24 hours of treatment using the Caspase-Glo 3/7 Assay System (Promega) on a BMG CLARIOstar plate reader ...
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bioRxiv - Cancer Biology 2024Quote: ... The activity of caspase 3/7 was measured after 48 h using the Caspase-Glo® 3/7 assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a total of 18 RNA extractions (3 morphs × 3 tissues × 2 biological replicates) were performed using the SV Total RNA Isolation System (Promega) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... and digested with 3 μg LysC (Wako) O/N at 37°C and then with 3 μg of trypsin (Promega) for eight hours at 37°C following FASP procedure (Filter-aided sample preparation 48) ...
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bioRxiv - Cell Biology 2024Quote: ... caspase-3/7 activities in cells were measured using a luminometric assay kit Caspase-Glo 3/7 (Promega, Madison, WI). The amount of luminescence was measured on the Victor3™ multilabel reader (PerkinElmer Inc.).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Initial P0 virus was obtained by addition of ∼3 µg recombinant bacmid DNA mixed with 3 µl FuGENE HD Transfection reagent (Promega) in 100 µl Sf900 II media (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... Caspase 3/7 activity was measured 16 and 24 h later using the Caspase-Glo 3/7 Assay System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... secondary antibody staining was performed for 1 h at room temperature using anti-rabbit HRP or anti-mouse HRP secondary antibodies (Promega, 1:10,000) in blocking buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). Membranes were developed and visualized using the Pierce™ ECL immunoblotting substrate (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... 30 mM Tris-HCl (pH 7), 1% Triton X-100, 1% NaDOC, 100 μg/mL cycloheximide (Applichem, Germany) and 30 U/mL RNase Inhibitor (Promega, United States)] ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg trypsin (Promega, Tokyo, Japan) overnight at 37°C on a shaking incubator ...
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bioRxiv - Microbiology 2021Quote: ... The CaptureSelect Biotin anti-C-tag Conjugate (LifeTechnologies #7103252100; 1/4000) was mixed with Streptavidin Alkaline Phosphatase (Promega #V5591; 1/1000 dilution) for 10 minutes before use ...
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bioRxiv - Cancer Biology 2020Quote: ... and diluted to 1 M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8 hr, Promega cat # V5113). After digestion ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were co-transfected in a 1:1 ratio with either human D1 or D2Long receptor and a split-luciferase based cAMP biosensor (GloSensor, Promega, Durham NC). The next day ...
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bioRxiv - Microbiology 2020Quote: ... The protein mixture was diluted four times and digested with LysC at 1:50(w/w) for four hours and then with Trypsin at 1:40 (w/w) (Promega, http://www.promega.com/) overnight.
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bioRxiv - Molecular Biology 2022Quote: ... we prepared a master mix containing the membrane-permeable Nluc substrate and a membrane-impermeable Nluc inhibitor using a 1:1000 dilution of Extracellular NanoLuc Inhibitor and a 1:333 dilution of NanoBRETTM Nano-Glo Substrate into PBS (Promega, Madison, WI). Aliquots of the Nluc substrate/inhibitor master mix (100 µl ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were digested with 1 μg of lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg of trypsin (Promega, Tokyo, Japan) overnight at 37 °C in a shaking incubator ...
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bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
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bioRxiv - Cell Biology 2023Quote: ... and 10μg of pLKO.1-mCherry-scrambled shRNA or pLKO.1-mCherry-IRE1α shRNA using FuGENE-HD reagent (Promega, Madison, WI, USA). After 24 h of transfection ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The crude nuclei were pelleted and resuspended in 400 μl nuclei lysis buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA, 1% SDS, 1× EDTA-free Proteinase Inhibitor, and 1000 U RNase I Promega, Madison, WI, USA). Supernatant obtained after sonication and centrifugation steps were digested with DNase I for 20 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... blots were incubated for 1 h at room temperature with the corresponding horseradish peroxidase-conjugated antibody (1:2000; Promega, Madison, WI, USA) and washed again with TBS-T ...
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bioRxiv - Plant Biology 2024Quote: ... columns were pre-equilibrated with 5 CV of 0.1 M NH4CO3 and then loaded with 1 CV of 0.1 M NH4CO3 containing 1 µg/ml trypsin gold (Promega V5280, Promega, USA). The flow-through containing the digested peptides of S-nitrosated proteins (elution 1 ...
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bioRxiv - Microbiology 2020Quote: ... 0.3% xylose and 1% low melting temperature agarose (Promega) on slides ...