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Citations for Promega :
1101 - 1150 of 4195 citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... One aliquot was used for DNA extraction using the Promega Wizard® Genomic DNA Purification kit (Promega Corporation, Madison, WI, USA) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... One µL of lysate was then added to make 20µL PCR reactions with GoTaq G2 Green Master Mix (Promega, Cat# M7823) and 1µM forward and reverse primers (Table 1) ...
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bioRxiv - Microbiology 2023Quote: ... and cellular expression of the luciferase reporter indicating viral infection was determined using the ONE-Glo™ Luciferase Assay System (Promega) 48 h after infection ...
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bioRxiv - Microbiology 2023Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2.5 h at 37° C with 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... the cleaned PCR product was eluted from the beads in 10 µL of buffer solution containing 10 mM Tris-HCl pH 8.0 with 50 mM NaCl and the DNA concentration was quantified on a Quantus Fluorometer using Quantifluor ONE dsDNA dye (Promega #E4891).
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 15,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx or DjCas13d using ViaFect™ Transfection Reagent (E4981, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected at > 80% confluence with all-in-one PiggyBac plasmids containing different Cas13d orthologs using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 15,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx or DjCas13d using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected at > 80% confluence with all-in-one PiggyBac plasmids containing different Cas13d orthologs using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of the dilution was mixed with 20 μL of passive buffer which was then mixed with 30 μL ONE-Glo™ EX Luciferase Assay Reagent (Promega) and incubated at room temperature for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was assessed using the MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation, G3580, Promega, Madison, WI, USA). In brief ...
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bioRxiv - Genomics 2023Quote: ... TRs were amplified using the forward and reverse primers plus an M13 primer with one of the four fluorophores with GoTaq polymerase (Promega #PRM7123) using PCR program ...
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bioRxiv - Biophysics 2023Quote: To investigate CBD influence on the cells viability and metabolic activity the cells were tested using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega) with tetrazolium compound ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell viability was measured 72 hr after drug treatment using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The MTS assay was carried out at certain time points using The CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega #G3580) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Cytotoxic activity of the CAR T cells was evaluated with the Promega ONE-Glo™Luciferase Assay (Promega Corporation; cat# E6120) according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... the morphology of the cells will be assessed and MTS tetrazolium compound (CellTiter 96 AQueous One Solution Assay, Promega, Madison, WI) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were cultured for 72 hours and then measured for cell growth using an MTS assay (CellTiter 96® AQueous one solution cell proliferation assay, Promega). The IC50 values were generated from 3 independent experiments using Prism 8.0 (GraphPad ...
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bioRxiv - Cell Biology 2024Quote: ... cells were gently washed once with 1X DPBS and MTS reagent of 20 μL (Cell Titer-96 Aqueous One Solution, Promega, USA) in 80 μL of cell culture media for 3 h (37°C and 5% CO2) ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were incubated for one hour at 47°C with 2 µg of sequencing grade trypsin (Promega, San Luis Obispo, CA) dissolved in 50 mM TEAB ...
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bioRxiv - Biochemistry 2024Quote: ... Cell proliferation was evaluated using a Promega CellTiter 96™ AQueous One Solution Cell Proliferation Assay (MTS) as per the manufacturer’s instructions (Promega G3580). The absorbance (490 nm ...
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bioRxiv - Bioengineering 2024Quote: One hundred micrograms of each antibody were reduced with 50 mM DTT and treated with 2 µl PNGase F (Promega, USA) at 37°C for over 16h with stirring before being transferred to a new vial for LC-MS analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA samples were quantified by using Thermo Scientific NanoDrop One and 100ng of cDNA as template for GoTaq® Green Master Mix (Promega) 35 cycles of PCR amplification with annealing at 61°C ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were incubated for one hour at 47°C with a solution of sequencing grade trypsin (Promega, San Luis Obispo, CA) dissolved in 50 mM TEAB at a 1:25 (wt/wt ...
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bioRxiv - Microbiology 2020Quote: Cytotoxicity of the reconfirmed hits was determined by using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI) on A549 human lung carcinoma cells [40] ...
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bioRxiv - Neuroscience 2021Quote: ... One microgram of total RNA was transcribed to cDNA by reverse transcriptase enzyme action Improm Pre-II ® (Promega, Madison, Wisconsin, USA). Quantitative RT-PCR reaction in real time was done on an ABI Prism ® 7500 Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: ... cell viability was measured using the CellTiter 96® Aqueous One Solution Cell Proliferation Assay according to manufacturer specifications (Promega, Madison, WI).
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bioRxiv - Physiology 2022Quote: Assays involving a range of peptides from 14mer down to 6mers assessed total viable PC3 or PNT2 cells using a 96-well plate MTS colorimetric assay (CellTiter 96 Aqueous One Solution Cell Proliferation Assay, Promega, Southampton, UK), with absorbance measured at 492 nm ...
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bioRxiv - Physiology 2022Quote: ... or only administration (LNCaP) total viable cell numbers were measured colorimetrically (MTS) using a CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega, Southampton, UK), following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: MTS assays of HCoV-229E experiments were performed using the The CellTiter 96® AQueous One Solution Cell Proliferation Assay kit (Promega, #G3582). In brief ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then incubated for 24 hours at 37°C in 5% CO2 and the luciferase activity was measured using the ONE-Glo™ luciferase assay (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The cells were incubated at 37°C in 5% CO2 for 24 hours and luciferase activity was measured using a ONE-Glo™ luciferase assay (Promega, USA) according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 48h post-transduction the luciferase activity was read by adding luciferase substrate (ONE-Glo EX luciferase assay system, Promega, Wisconsin, USA) using a luminescence plate reader (Envision ...
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bioRxiv - Cancer Biology 2021Quote: The effect of P2RX7B expression and inhibition on OS cell proliferation was assessed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, UK), TE85 ...
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bioRxiv - Microbiology 2020Quote: ... medium was removed from the cells and replaced by MTS reagent (CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega, Madison, WI) diluted in PBS ...
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bioRxiv - Microbiology 2021Quote: ... was performed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay kit using manufacturer’s instructions from Promega (Madison, WI, USA). Vero E6 cells were seeded in 96-well plates at a density of 10,000 cells/well ...
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bioRxiv - Genomics 2021Quote: ... The barcoded pool was sized on a Agilent Tapestation D5000 tape and quantified using QuantiFluor® ONE dsDNA System (Promega, WI, USA) and the molarity calculated.
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bioRxiv - Biochemistry 2023Quote: One-hundred microliters of 50 mM Tris-HCl (pH 8.0) and 500 ng of trypsin/Lys-C mix (Promega, cat. no. V5072) were added to the washed HaloTag ligand plate following the first-generation assay and mixed gently at 37 °C overnight to digest the proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... one of these plasmids is combined with a non-targeted firefly luciferase reporter pGL4-SV40 (Promega; for simplicity referred to as FL) and a Renilla luciferase reporter carrying a Mos cognate sequence complementary to MosIR-expressed dsRNA ...
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bioRxiv - Neuroscience 2023Quote: ... a subset of cells underwent viability testing via MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega, Catalog #G3582) or ATP assay (CellTiter-Glo® Luminescent Cell Viability Assay ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured for 1 day in 96-well plates and treated with small molecules for 3 consecutive days prior to incubation with 120 μL fresh media containing 20 μL of CellTiter 96® AQueous One Solution (Promega, G3580) for 1 to 4 hours at 37°C in a humidified ...
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bioRxiv - Bioengineering 2023Quote: ... the liquid DMEM culture medium and the hydrogel overlaying the cultured chondrocytes were first removed before introducing 300 µL of CellTiter 96® Aqueous One Solution Cell Proliferation assay (Promega, Australia) working solution ...
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bioRxiv - Bioengineering 2024Quote: Cell viability of the inks screening assays and the bioprinted samples was assessed using the 96®AQueous One Solution Cell Proliferation assay (MTS assay, Promega, USA). The supplier protocol was used ...
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bioRxiv - Molecular Biology 2024Quote: One hundred micrograms of urinary proteins from each sample were digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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bioRxiv - Immunology 2024Quote: Toxicity was evaluated by measuring the lactate dehydrogenase (LDH) level in cell supernatants using the CytoTox-ONE™ Homogeneous Membrane Integrity Assay kit (Promega, G7892) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured 72 h after the drug treatment using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Spain). PBS-treated cells were used as control ...
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bioRxiv - Bioengineering 2024Quote: ... Relative firefly luciferase activity and cell viability were assessed ∼24 h after LNP addition using ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay (Promega, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... The cell proliferation was analyzed after 48 hours culture using CellTiter One Solution Cell Proliferation Assay kit (MTS assay, Promega, Cat# G8461) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The A2780 cell proliferation was analyzed 48h after cultured in CM using CellTiter One Solution Cell Proliferation Assay kit (MTS assay, Promega, Cat# G8461) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Cell growth and viability were assessed using the MTS CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, Wisconsin, US) after 72 hours of treatments ...