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Citations for Promega :
1051 - 1100 of 1351 citations for Zika Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
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bioRxiv - Microbiology 2022Quote: ... Proteins on magnetic beads were digested overnight at 37°C with 1μl (0.2 μg/μL) of trypsin (Promega) in a 25-mM NH4HCO3 buffer per sample ...
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bioRxiv - Biochemistry 2020Quote: ... Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega, Madison, WI) supplemented with canine pancreatic rough microsomes (tRNA probes ...
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bioRxiv - Cancer Biology 2019Quote: ... Dilute proteins were digested overnight with 5 μl of 25 ng/μl trypsin gold (Promega, Madison, WI, USA) and desalted with a ZipTipC18 pipette tip (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... and PERP plus any interacting proteins were cleaved from the resin using 30 units of ProTEV enzyme (Promega) at room temperature for one hour (elution 1) ...
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bioRxiv - Neuroscience 2019Quote: ... Resins were transferred to a new tube and proteins were digested with 0.1 µg Lys-C for 2h at 37°C and 0.5 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: The protein samples were digested with 500 ng of sequencing grade trypsin (V511, Promega Co., Madison, WI, USA) and incubated at 37 °C following the protocol described by Shevchenko et al.22 ...
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bioRxiv - Cell Biology 2019Quote: ... In vitro translated (IVT-) proteins were synthesized using a TNT quick-coupled transcription-translation kit (Promega, Madison, WI). For synthesizing the Pav and PavDEAD proteins ...
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bioRxiv - Plant Biology 2020Quote: ... LUC activity was measured with 20 μl of protein sample and 100 μl of LUC assay reagent (Promega). Activation from the DHS was calculated as (LUC luminescence/rate of MUG accumulation ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were then digested overnight at 37°C with 0.2 μg of modified sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested on the column with 0.25 µg of Trypsin/Lys-C protease mix (Promega; cat. V5071), and peptides eluted for mass spectrometry.
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bioRxiv - Microbiology 2019Quote: ... The membranes were washed with TBST and incubated with 200-fold diluted LgBiT protein in TBST (N2421, Promega), for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Halo-tagged proteins were labeled by incubating cells with 200nM HaloTag Ligand conjugated to Janelia Fluor 646 (Promega) for 30 min before imaging ...
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bioRxiv - Cell Biology 2021Quote: The Rosa26 promoter and the 5’UTRs of secretory granule protein transcripts were subcloned into the pNL1.1 (Promega) using standard cloning techniques ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... purified protein samples were prepared by S-alkylation with iodoacetamide and digestion with either with LysC/GluC (Promega) [RBD] or ...
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bioRxiv - Biochemistry 2022Quote: ... laevis TRAIP that was produced in the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The HaloTag-CpOGA proteins were mixed with equal molar of HaloTag Alexa Fluor 488 or 660 ligands (Promega) and incubated at room temperature for 30 min ...
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bioRxiv - Physiology 2022Quote: ... Luciferase activities were measured and plotted relative to lysate protein concentrations using the Promega Dual Luciferase Reporter (Promega) assays according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were detected with horseradish peroxidase (HRP)-conjugated rabbit or mouse secondary antibody [1:1,000 dilution] (Promega). The chemiluminescence signal was imaged using an Odyssey XF Imager (LI-COR ...
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bioRxiv - Neuroscience 2023Quote: ... Digestion reaction for proteins was initially set up with 1 μg of MS grade LysC (Promega, Cat# PI90307) for 3 hours at RT and then continued overnight with addition of 2 μg of trypsin (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... flag-tagged MED25 protein and CFP-tagged RAP2.2 and RAP2.12 proteins (Schmidt et al., 2018) were expressed using TNT SP6 High Yield Wheat Germ (Promega) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 30 µg of proteins from lysed cells were digested with 500 units of endoglycosidase H (ENDO H, Promega) and 10 units of Peptide N-glycosidase F (PNGase F ...
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bioRxiv - Cell Biology 2023Quote: 35S-radiolabeled Tom40 precursor proteins were synthesized in vitro using the TNT Quick Coupled Transcription/Translation kit (Promega). Transcripts for MCO6 encoding 3 additional N-terminal methionine residues for radiolabelling of the Mco6 protein which lacks internal methionine residues ...
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bioRxiv - Cell Biology 2023Quote: ... the gel plugs were digested by using 1:50 (Trypsin: Protein amount ratio) Sequencing Grade Modified Trypsin (Promega) at 37 °C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested off the beads by adding 6 μL of 1 μg/μL sequencing grade Trypsin (Promega), diluted in 50 mM acetic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were directly digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested in 100 mM ammonium bicarbonate containing 2 μg of trypsin/Lys-C enzyme mix (Promega) at 37 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: Reduced and alkylated proteins were prepared by in-gel digesting overnight at 37°C using 100ng trypsin (Promega). The supernatant was dried by vacuum centrifugation and the samples were resuspended in 0.1% trifluoro acetic acid ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg protein was used for dual luciferase transactivation assay using Dual-Luciferase® Reporter Assay System (Promega) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... immunodepleted protein samples were successively digested with LysC (0.008 μg/μL, Wako) and trypsin (0.002 μg/μL, Gold, Promega), using a filter-aided sample preparation (FASP ...
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bioRxiv - Plant Biology 2023Quote: ... In-gel digestion of proteins was performed at 37 °C overnight using 2.5 μg Trypsin/Lys-C (Promega). Samples were further digested with 2.5 μg Chymotrypsin (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... LUC activity was measured with 20 mL of protein sample and 100 mL of LUC assay reagent (Promega). Promoter activation was calculated as (rate of MUG accumulation / LUC luminescence ...
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bioRxiv - Cell Biology 2024Quote: ... precipitated proteins were resuspended in 0.1% RapiGest with 6 μL of sequencing-grade trypsin (Promega, 0.5 μg/μL) added to each sample and digested overnight at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... LUC activity was measured with 20 μl of protein sample and 100 μl of LUC assay reagent (Promega) using Clariostar plate reader ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with LysC (Wako) (substrate:enzyme=100) overnight at 37°C and then with sequencing grade trypsin (Promega) (substrate:enzyme=100 ...
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bioRxiv - Cell Biology 2020Quote: ... securin-CFP was in vitro translated using the TNT SP6 high-yield wheat germ protein expression system (Promega, #L3261). Three or 4 µL of in vitro translated securin-CFP was added to 50 or 80 µL extract ...
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bioRxiv - Immunology 2021Quote: ... After 8 days of transfection mAbs were purified from culture supernatant using Magna Protein A beads (Promega, WI, USA).
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bioRxiv - Microbiology 2019Quote: ... amyloliquefaciens were determined by SDS-PAGE (10% gel) and was compared with the standard protein marker obtained from Promega, USA (Fig ...
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bioRxiv - Molecular Biology 2021Quote: ... CspA was allowed to bind cspA mRNA in 40 µL of Buffer N by incubating 10 pmoles of renatured mRNA with the indicated amounts of protein CspA in the presence of 0.4 U/µL of RNasin (Promega). After 15 min at 15°C ...
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bioRxiv - Biochemistry 2020Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). Digested proteins were finally desalted using a C18 TopTip (PolyLC ...
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bioRxiv - Plant Biology 2019Quote: ... HA- and GST-tagged proteins were synthesized in vitro using the TNT® Coupled Wheat Germ Extract Systems (Promega). For the pull-down assays ...
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bioRxiv - Molecular Biology 2019Quote: ... and C-terminally truncated VP16 (VP16ΔC) proteins were synthesized using a rabbit TNT quick-coupled transcription/ translation system (Promega) with 35S-labeled methionine ...
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bioRxiv - Cell Biology 2019Quote: ... the purified C-I30-Flag protein (30µl beads) was digested on beads (Anti-FLAG M2 affinity gel) with 10U ProTEV Plus (cat#: V6101, Promega) in the 1x ProTEV digestion buffer (50 mM HEPES pH 7.0 ...
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bioRxiv - Cancer Biology 2019Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). Finally ...
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bioRxiv - Cell Biology 2019Quote: Target proteins were in vitro transcribed and translated in rabbit reticulocyte lysate (TNT-coupled reticulocyte system; Promega; #L4600, #L4610) supplemented with 35S-Methionine (IsoLabel L-35S Steady Blue ...
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bioRxiv - Immunology 2021Quote: ... The antibodies were purified from the culture supernatant 4 – 6 days later using protein G magnetic beads (Promega #G7472). Purified antibodies and antibody elution buffer (5 parts elution buffer (100 µM glycine-HCl ...
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bioRxiv - Systems Biology 2020Quote: ... The solution was diluted to below 1M Urea using 200mM bicarbonate buffer and an aliquot of approximately 25μg protein were digested using sequencing grade Trypsin (V511A, Promega) at 37°C over-night (Trypsin to protein ratio of approx ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Biochemistry 2020Quote: ... Protein (100 μg) digestion was performed by the FASP method (Tang et al., 2017) utilizing trypsin (Promega, Madison, WI) and 0.1M tetraethylammonium bromide (TEAB ...