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Citations for Promega :
1051 - 1100 of 1259 citations for Usutu Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and the protein pellet was resuspended in 50 mM ammonium bicarbonate and digested with sequencing-grade modified trypsin (Promega) overnight at 37°C ...
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bioRxiv - Physiology 2022Quote: ... The proteins were cleaved into tryptic peptides by addition of 25 μl of a 0.2 μg/μl trypsin (Promega) in 50 mM ammonium bicarbonate and incubation (18 h ...
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bioRxiv - Plant Biology 2022Quote: ... ZmEREB57 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260) (Promega), and then purified using Magne HaloTag Beads (G7281 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we dissolved 10 μg of protein in 10 μl 10 M urea containing 0.1% ProteasMax (Promega. Madison, WI, USA). Cysteine residues were reduced with 5 mM DTT (30 min at 50 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Bands corresponding to the protein of interest were cut from the gel and in-gel digestion with trypsin (Promega) was performed essentially as described in 41 ...
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bioRxiv - Cancer Biology 2023Quote: ... Unlabeled proteins were cell-free-synthesized using the TNT T7 Quick Reaction system according to the manufacturer instructions (Promega) and incubated with purified GST fusion proteins coupled with glutathione sepharose 4B fast flow beads (GE healthcare ...
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bioRxiv - Molecular Biology 2023Quote: Electrophoretic Mobility Shift Assays were performed using proteins expressed in the TNT T7 Coupled Reticulocyte Lysate System (Promega, WI), according to the manufactureŕs recommendation ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were rinsed using 80% EtOH and proteins were digested with 100 µL of trypsin in 50 mM TEAB pH 7.55 (Promega, protein:enzyme ratio 1:50 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were further diluted with 50 mM Tris pH 7.9 pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 µg trypsin (Promega) (1/100 ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 ng/well of chaperone RTP1S,105 50 ng/well of the G protein subunit Gαolf,106–107 olfactory G protein subunit Gγ13,108 and 50 ng/well of pGloSensorTM-22F (Promega, Madison, USA).109 The utilized pGloSensor™-22F is a genetically engineered luciferase with a cAMP-binding pocket ...
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bioRxiv - Genetics 2023Quote: ... proteins were extracted by chloroform-methanol precipitation and digested with 2 μg trypsin/Lys-C mixture (Promega, Madison, WI). Samples were labeled with 10-plex tandem mass tag reagents (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were diluted 1:3 with 50 mM HEPES pH 8.5 and then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...
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bioRxiv - Plant Biology 2024Quote: ... and the recombinant protein was produced using a TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA). Magne Halo Tag Beads (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... urea was diluted to 1 M and proteins digested overnight with modified sequencing grade trypsin (Promega, Madison, WI, USA) at a 1:50 enzyme:protein ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... The identity of each cell line was confirmed by DNA fingerprinting via short tandem repeats at the time of mRNA and total protein lysate preparation using the PowerPlex 1.2 kit (Promega).
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bioRxiv - Plant Biology 2020Quote: HALO-FDL1 and HALO-GST (negative control) DAP-seq samples were performed using protein expressed in the TNT rabbit reticulocyte expression system (Promega) as previously described (Bartlett et al. ...
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bioRxiv - Biophysics 2020Quote: Plasmid DNAs containing Tol2 vectors comprising GV genes and fluorescent protein genes described above and transposase mRNA were introduced to KPL-4 cells by lipofection using FuGene HD (Promega) in OptiMEM (Gibco) ...
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bioRxiv - Developmental Biology 2020Quote: In vitro translation (IVT) with 500 ng of mRNA was performed using the TnT SP6 High-Yield Protein Expression System (Promega) to synthesize tagged proteins ...
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bioRxiv - Molecular Biology 2019Quote: ... sliced and dehydrated twice using 50% ACN followed by 100% ACN and the proteins digested with ~100 µl of Trypsin solution (10 µg/ml, Promega) in 25 mM ammonium bicarbonate by incubating overnight at 37° C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells expressing HT-fusion proteins or HT-Ctrl were incubated in the mammalian lysis buffer supplemented with Protease Inhibitor cocktail (Promega) and RQ1 RNase-Free DNase (Promega ...
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bioRxiv - Neuroscience 2019Quote: ... Luciferase assays (Firefly and Renilla) were done on the protein extracts according to manufacturer instructions (Dual-Luciferase Reporter Assay System, Promega). The signal was normalized to the amount of protein in the sample as determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... SDC concentration was adjusted to 1% and 120 µg of proteins from each bacterial culture were digested by addition of trypsin (Promega) in a 1:50 (enzyme:protein ...
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bioRxiv - Physiology 2019Quote: ... The concentrated urea was diluted 12 times with 50 mM ammonium bicarbonate buffer and proteins were digested for 18 hours at 36°C using trypsin/LysC mixture (Promega) in 1:50 (w/w ...
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bioRxiv - Molecular Biology 2020Quote: ... Cysteines were then alkylated with 0.5 M iodoacetamide in 100 mM NH4HCO3 (45 minutes in a dark room at room temperature) and proteins were digested overnight with 10 ng/μl trypsin (CAT NO V5280, Promega) in 25 mM NH4HCO3 77 ...
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bioRxiv - Biochemistry 2019Quote: ... Cells between passage 10 – 30 grown in 40-mm wells were transiently transfected at 30 – 50% confluency with plasmids encoding for TRPM2DR and green fluorescent protein (GFP) using FuGene6 (Promega). Transfected cells were subcultured onto laminin (Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... Cells were transfected with target channel-cloned pBiEX vectors and enhanced green fluorescent protein (EGFP)-cloned pBiEX vectors using Fugene HD transfection reagent (Promega). The channel-cloned vector (2 μg ...
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bioRxiv - Microbiology 2019Quote: Protein digestion - for samples processed with reagent 1 and 2 as well as for supernatants (proteomes) MS grade trypsin (Promega) was used at 1:1000 w/w protease:protein ...
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bioRxiv - Immunology 2019Quote: ... Expression of RLuc fusion proteins was estimated by measuring the luminescence of the cells after incubation with 5 µM coelenterazine H (Promega). In parallel ...
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bioRxiv - Bioengineering 2020Quote: ... In-solution protein digestion was carried out in a ratio of 1:25 w/w Trypsin/Lys-C (Mass Spectrometry Grade, Promega) to protein overnight at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Remaining iodoacetamide was quenched by adding 5 mM DTT and the proteins were digested with 150 ng trypsin (Trypsin Gold, Promega) at room temperature for 90 min ...
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bioRxiv - Pathology 2020Quote: ... Sample pH was adjusted to 7.5 with 0.05 M TEAB and proteins were digested overnight at 37 °C with sequencing-grade trypsin (Promega, Madison, WI) in 1:30 (w/w ...
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bioRxiv - Immunology 2021Quote: ... The pellet then was resuspended in PBS for NTA or 1X Passive Lysis Buffer (PLB) for RNA or protein estimate (Promega). For EV associated RNA and protein ...
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bioRxiv - Immunology 2021Quote: ... dithiothreitol was added to a final concentration of 50 mM and proteins were digested overnight with sequencing grade trypsin (Promega) at an enzyme-to-substrate ratio of 1:50 at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... Proteins separated on the second gel were transferred to a PVDF membrane and DivJ levels were measured using Western blots by probing the protein with anti-HaloTag antibody (Promega). Due to the requirement of growing cells in low phosphate media ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were transferred by semi-dry Western Blot onto PVDF membranes visualised with polyclonal antibody and BCIP/NBT (Promega, S3771).
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bioRxiv - Plant Biology 2021Quote: ... Halo fusion proteins were produced from 1.5 µg plasmid DNA in a 15 µL reaction using TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega). Protein expression was confirmed by Western blot with Anti-HaloTag monoclonal Ab (1:2000 ...
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bioRxiv - Biochemistry 2021Quote: A total of 100 μg of protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using FASP methods [24] ...
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bioRxiv - Biochemistry 2021Quote: Pulldowns with cellular extracts were carried out as described for GFP-immunoprecipitations using 1-2 μg of GST-fused proteins bound to glutathione magnetic beads (Promega). For immunoprecipitations of recombinant proteins ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were diluted 10-fold in 50 mM Tris-HCl and digested with Sequencing Grade Modified Trypsin (Promega - V5111) at a Protein:Trypsin ratio 50:1 overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were re-solubilized in 6 M urea buffer followed by reduction and alkylation before digestion with trypsin (Promega) at 37°C for 18 hr ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were diluted with 50 mM NH4HCO3 to a final concentration of 2M urea and digested with trypsin (Promega, UK), at an enzyme-to-substrate ratio of 1:20 ...
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bioRxiv - Cell Biology 2021Quote: ... The amount of HiBiT proteins in the concentrated EV fraction was measured using the Nano Glo HiBiT Lytic Detection System (Promega). EV marker proteins in the concentrated EV fraction were detected by western blotting as described above.
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bioRxiv - Cell Biology 2021Quote: ... Trypsin digestion was carried out overnight at 37°C with 1:50-1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The remaining iodoacetamide was quenched by adding 5 mM DTT and the proteins were digested with 150 ng trypsin (Trypsin Gold, Promega) for 90 min at room temperature in the dark ...
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bioRxiv - Biochemistry 2022Quote: ... Remaining iodoacetamide was quenched by adding 5 mM DTT and the proteins were digested with 200 ng trypsin (Trypsin Gold, Promega) at 37 °C ON ...
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bioRxiv - Biochemistry 2022Quote: ... A total of 100 μg of protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins on beads were digested overnight at 37°C by sequencing grade trypsin (12.5 μg/ml; Promega Madison, Wi, USA) in 20 μl of NH4HCO3 25 mmol/l ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were diluted with ddH2O 1:5 and proteins were digested for 20 h at 37°C using trypsin (Trypsin Gold, Promega) at an enzyme/protein ratio of 1:50 ...
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bioRxiv - Biochemistry 2022Quote: Radiolabeled proteins were synthesized in the presence of 35S-methionine and 35S-cysteine using either coupled reticulocyte lysate system (TNT, Promega) or uncoupled reticulocyte lysate system (Promega) ...
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bioRxiv - Plant Biology 2022Quote: ... The Halo fusion proteins were produced using 1.2 μg plasmid DNA of the respective pIX-HALO construct in a volume of 15 μl using the TNT SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega). Protein expression was confirmed by Western blot with Anti-Halo Tag monoclonal antibody (1:2000 ...