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Citations for Promega :
1051 - 1100 of 1579 citations for Recombinant Mouse COL3A1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... The proteins were diluted to 0.1 M ammonium bicarbonate and digested overnight with Trypsin (Promega, V5113) at 37°C with a protein-to-enzyme ratio of 50:1 ...
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bioRxiv - Biophysics 2023Quote: ... the proteins were digested into tryptic peptides by incubation with 1□µg sequencing grade trypsin (Promega) overnight at 37□°C ...
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bioRxiv - Neuroscience 2023Quote: ... Syp-Halo protein was incubated with 5 times molar excess halo ligand Alexa 488 (Promega, USA) overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein aggregation was initiated by addition of Factor Xa protease (SKU PR-V5581, Promega, Madison, WI) at 22◦C ...
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bioRxiv - Neuroscience 2023Quote: ... After the sequential digestion with LysC and trypsin (Promega, each protease to protein ratio 1:80), beads were retained with a magnetic rack and samples were filtered with Costar Spin-X spin filters (0·22 µm cut-off ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein was quantified using the Nano-Glo® HiBiT Extracellular Detection System (N2420, Promega, Madison, WI) using the HiBiT Control Protein (20 µM ...
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bioRxiv - Biophysics 2023Quote: ... The purified fused protein was cleaved by treating it with Factor Xa (FXa; Promega, Madison, WI) at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... and proteins were digested overnight with LysC (FUJIFILM Wako) and trypsin (sequence grade; Promega, Madison, WI) at the protein:enzyme ratio of 100:1 for each enzyme at 25 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µg of reconstituted protein was digested in 0.05 N HCl with 1 µg pepsin (Promega) without prior reduction/alkylation at a volume of 200 µl 50 °C for 3 h ...
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bioRxiv - Systems Biology 2023Quote: ... pH 8.0 were added and proteins were digested with 1.5 μl trypsin (0.5 μg/μl; Promega) with constant shaking at 1,100 rpm and incubation at 37 °C on a ThermoMixer C incubator for 4 h ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: Proteins were digested by incubating each gel slice with 10 ng/µl of trypsin (V5111, Promega) in 40 mM NH4HCO3 ...
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bioRxiv - Microbiology 2023Quote: ... The protein extracts were digested by Lys-C endoproteinase and then by trypsin (Trypsin Gold, Promega). Peptides were vacuum-dried and resuspended in 100 µl of solvent A (0.1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega). Translation reaction products from 0.25 μg of plasmid DNA were incubated with GST-labeled proteins on glutathione-Sepharose beads in NETN-E buffer (50 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... the pcDNA3.1+ expression plasmids for spike proteins were co-transfected using Fugene (Promega, Madison, WI, USA) into HEK293T/17 cells ...
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bioRxiv - Systems Biology 2024Quote: ... proteins were digested overnight at 37 °C and 800 rpm using sequencing-grade modified trypsin (Promega) at a protein-enzyme-ratio of 50:1 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples (15 µg) were digested overnight at 37 °C with trypsin (Promega, Madison, WI-USA) in a protein:trypsin ratio of 50:1 (w/w) ...
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bioRxiv - Cancer Biology 2024Quote: ... the fusion proteins were expressed using the cell-free transcription and translation system (Cat. # L4140, Promega) following the manufacturer’s protocol ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Sigma-Aldrich)–coated dishes or glass coverslips with 30 ng/ml of 2.5 S mouse NGF (G5141, Promega) in the Neurobasal medium containing B27 supplement (17504044 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by the incubation with a secondary antibody anti-mouse IgG HRP conjugate (W4021, Promega, U.S.A.). All the primary antibodies were diluted 1/3000 in 10% Blocking One/TTBS (50 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: Primary antibodies and the dilutions used are as follows: anti-Halo (mouse, Promega G9211, 1:200); anti-Ezh2 (mouse ...
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bioRxiv - Microbiology 2021Quote: ... immunoblots were probed with the appropriate secondary α-mouse antibody conjugated to horseradish peroxidase (Promega, W402B) at a dilution of 1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... all cells were incubated in the following: mouse anti-βIII tubulin (1:1000; 2 hr; Promega) and goat anti-mouse Alexa 546 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega. Chemiluminescence images were acquired using the ImageQuant LAS 500 system (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: HepG2 cells or frozen mouse liver were sonicated in PBS containing protease and phosphatase inhibitors (Promega) in an ice-water bath using a Branson 250 digital sonifier programmed to cycle at a 10% amplitude with 5 seconds on followed by 20 seconds off ...
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bioRxiv - Cancer Biology 2019Quote: ... were secondary incubated with anti-mouse IgG (H+L) horseradish peroxidase coupled (1:3,000, W402b, Promega) and polyclonal anti-rabbit IgG (1:5000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies used were: rabbit and mouse anti-βGal (1/1000; Promega and MP Biomedicals, respectiveley), rat anti-Ser (1/1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... and then with 1:5000 of anti-rabbit (#W401B) or anti-mouse (#W402B) secondary antibodies (Promega).
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bioRxiv - Genetics 2019Quote: ... As secondary antibodies horseradish peroxidase (HRP)-conjugated α-mouse or α-rabbit IgG (Promega, 1:4000) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary horseradish peroxidase-conjugated anti-mouse or anti-rabbit rabbit IgG antibody (Promega #W4021 and #W4011) were used at 1:2,500 dilution followed by detection by ECL (SuperSignal West Pico Chemiluminescent Substrate ...
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bioRxiv - Cell Biology 2022Quote: ... washed and incubated with secondary antibody goat-anti-rabbit-HRP or goat-anti- mouse-HRP (Promega) for 1 h ...
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bioRxiv - Immunology 2022Quote: ... mixed with Lumit BiT Set 2 (anti-mouse Ab-SmBiT, anti-rabbit Ab-LgBiT, Promega W1220) and added for 90min ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies were horseradish peroxidase-conjugated anti-mouse antibodies (cat. no. W402B; Promega, Madison, WI, USA) or rabbit IgG (cat ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then incubated with an alkaline phosphatase-conjugated anti-mouse IgG (Promega, Madison, WI, USA) as the secondary antibody ...
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bioRxiv - Biochemistry 2023Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega. Chemiluminescence images were acquired using the ImageQuant LAS 500 or 600 system (GE Healthcare).
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bioRxiv - Genetics 2023Quote: ... and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP, Promega). In order to detect the endogenous Ptiwi09 protein ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated for another hour with an anti-Mouse IgG HRP-conjugate secondary antibody (Promega) (diluted 1:10 000 in PBST) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega for chemiluminescence imaging ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were renatured and assayed according to the Nano-Glo® In-Gel Detection System protocol (Promega). Light produced by NLuc was captured using a Chemidoc MP Imager (Biorad).
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bioRxiv - Genetics 2021Quote: ... protein samples were digested overnight at 37°C with 125 ng modified porcine trypsin (Trypsin Gold, Promega). Peptides were extracted under acidic conditions and dried out using a speedvac concentrator ...
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bioRxiv - Microbiology 2021Quote: Peptides from cell-surface/biofilm-ECM-associated proteins were released via a Trypsin/Lys-C mix (Promega) at a final concentration of 50 ng µL-1 ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant was collected and purification was done via His-tag using HisLink Protein Purification Resin (V8823, Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: In vitro transcription/translation assays were carried out in a S30 T7 HighYield Protein Expression System (Promega). The full lenght Hrnpk cDNA template was cloned from total RNA obtained from GH4C1 cells and transferred to a pET28a Escherichia coli T7 expression vector (Novagen ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG-GABARAPL2 were generated by subcloning coding sequences for each protein into pCI-neo vector (Promega, E1841) with N-terminal FLAG tag ...
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bioRxiv - Biochemistry 2019Quote: ... N-glycans were released from the membrane-bound protein using 2 U PNGase F (Promega, WI, USA) with overnight incubation at 37°C ...
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bioRxiv - Systems Biology 2019Quote: ... and enzymatic protein hydrolysis was initiated by the addition of trypsin (Sequencing Grade Modified Trypsin, Frozen, Promega) at a ratio of 1:50 ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion on beads was carried out using a ratio 1:50 sequencing grade modified trypsin (Promega): protein extract in 100 mM ammonium bicarbonate at 37 °C overnight ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was digested by incubation overnight at 37°C with trypsin (5 ng/ ml, Promega, Madison, WI) in 50 mM NH4HCO3 (pH 7.8) ...
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bioRxiv - Biochemistry 2019Quote: ... The slices were washed and dried before digestion of proteins with 20 ng/μl of trypsin (Promega) or 12 ng/μl of chymotrypsin (Roche ...
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bioRxiv - Bioengineering 2021Quote: Target proteins were in-vitro expressed using rabbit reticulocyte lysate (TNT-coupled reticulocyte system; Promega; #L4600, #L4610) supplemented with either 35S-methionine/S-L-cysteine mix (PerkinElmer ...
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bioRxiv - Genetics 2019Quote: ... The resulting BMP15 protein was revealed using Transcend non-radioactive translation detection system with chemiluminescent method (Promega) after reducing SDS-PAGE on a gradient (4-15% ...