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Citations for Promega :
951 - 1000 of 5335 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... PCR was performed in GoTaq Master Mix (Promega) according to standard procedures ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was performed with GoTaq DNA polymerase (Promega); primer sequences are shown in supplemental Table S1 ...
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bioRxiv - Molecular Biology 2020Quote: ... we ligated PCR amplicons to pGEM-T (Promega) vector system and confirmed that all picked clones encompassed TATA box deletion and guide6 reconstruction using Sanger sequencing.
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bioRxiv - Zoology 2020Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 ul MgCl ...
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bioRxiv - Zoology 2020Quote: ... 10 μL 5× PCR buffer (Gotaq flexi, Promega), 8 μL 25mM MgCl ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products were cloned into pGEM-T (Promega) and sequenced ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with GoTaq Master Mix (Promega) with 1ul cDNA template at 1:20 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... and cleaned again using PCR cleanup (Promega, A6754). Plasmid DNA inputs were used in a set of concentrations ranging from 0.5 nM to 10 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... with mutagenesis performed by PCR using PfU (Promega). All mutagenesis was confirmed by Sanger Sequencing (Source Bioscience).
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bioRxiv - Synthetic Biology 2022Quote: ... and 300 μM of PCR Nucleotide mix (Promega). The liposome suspensions were incubated at 30 °C in a thermocycler for the indicated time periods.
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bioRxiv - Plant Biology 2022Quote: ... PCR was performed using GoTaq DNA Polymerase (Promega) with a melting temperature of 55 °C for SpCas9 ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was conducted using GoTaq DNA polymerase (Promega). Primer sequences were as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR fragments were subcloned into pGEM-Teasy (Promega) and then moved as HindIII/NotI fragments into pCB4699 ...
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bioRxiv - Physiology 2022Quote: ... qRT-PCR was performed using SYBR Green (Promega). The primers used were (5’-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... PCRs were performed using GoTag DNA polymerase (Promega) and primers annealing either of the flanking exons to look at the pattern of splicing or exon-junction (EJ ...
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bioRxiv - Zoology 2022Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 μl MgCl ...
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bioRxiv - Zoology 2023Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 μl MgCl ...
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bioRxiv - Microbiology 2023Quote: ... Diagnostic PCR was performed using GoTaq polymerase (Promega).
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bioRxiv - Physiology 2023Quote: ... PCR amplification used GoTaq Hot Start Polymerase (Promega). PCR conditions were 95°C for 3 min ...
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bioRxiv - Genomics 2024Quote: ... PCR products were cloned into pCI-neo (Promega) downstream of the Luciferase2 gene under the control of a CMV promoter ...
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bioRxiv - Developmental Biology 2024Quote: ... and also with PCR Master Mix (M7502, Promega), Taq polymerase (EP0402 ...
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bioRxiv - Neuroscience 2024Quote: ... GoTaq quantitative PCR (qPCR) Master Mix (Promega; A6002) was used to conduct the qPCR ...
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bioRxiv - Zoology 2024Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 μl MgCl ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR fragments were cloned in pGEM-T (Promega), and the inserts were sequenced by Sanger sequencing (University of California Berkeley DNA Sequencing Facility) ...
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bioRxiv - Microbiology 2024Quote: PCR was performed using GoTaq Master Mix (Promega) using 5 ng of DNA extracted from RS collected after growth on MHA containing 0 or 32 μg/mL cefiderocol ...
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bioRxiv - Microbiology 2021Quote: ... and the Renilla and firefly luciferase activities were measured using GloMax®-Multi+ Detection System (Promega, Madison, WI, USA) with an Renilla-Juice kit and a FireFly-Juice kit (pjk ...
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bioRxiv - Microbiology 2021Quote: ... and the firefly and Renilla luciferase activities were measured using GloMax®-Multi+ Detection System (Promega Madison, WI, USA) with a Luciferase Assay Reagent II and a Stop & Glo® Reagent (Promega Madison ...
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bioRxiv - Immunology 2021Quote: ... Firefly luciferase activity was then measured with BrightGlo luciferase reagent and a Glomax-Multi+ Detection System (Promega, Southampton, UK). Pseudotyped virus neutralisation titres were calculated by interpolating the point at which there was either 50% or 80% reduction in luciferase activity ...
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m7G cap-eIF4E interaction stimulates polysome formation by enhancing first-round initiation kineticsbioRxiv - Biophysics 2021Quote: ... The translation reaction mixture for single-molecule detection was assembled according to the manufacturer’s instructions with the following modifications: 1) the RNase inhibitor RNaseIn (Promega) was used at a final concentration of 0.8 U/ul ...
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bioRxiv - Microbiology 2021Quote: Enzymatic activity was determined by GDP-Glo bioluminescent GDP detection assay with GDP-Fucose (Promega Corporation, Madison, WI USA). Aliquots of 3 µg of the affinity purified recombinant proteins were incubated with 600 µM GDP-Fuc ...
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bioRxiv - Systems Biology 2022Quote: ... NanoBRET™ Nano-Glo® Detection System and CellTiter-Glo® Luminescent Cell Viability Assay was purchased from Promega.
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bioRxiv - Cell Biology 2022Quote: ... the absorbance was read at 750 nm with the use of a GloMax®-Multi Detection System (Promega, UK), in order to determine the EV samples protein concentration ...
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bioRxiv - Microbiology 2020Quote: ... using the standard protocol where 50 uL of extract was added with 50 uL of GloTM Detection Reagent (Promega) in a white bottom 96 well plate (Corning) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysis and detection of firefly luciferase were performed according to the protocol recommended by the manufacturer (Promega Inc). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... The plate was incubated in the dark for 2 mins then read on a Glomax Multi+ Detection System (Promega) as above ...
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bioRxiv - Microbiology 2020Quote: ... The plate was incubated in the dark for 2 mins then read on a Glomax Multi+ Detection System (Promega) as above ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue supernatant were used to estimate BDNF (detection limits 7.8-500 pg/ml) by ELISA (BDNF Emax Immuno Assay System, #G7611, Promega), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were lysed and luminescence from each well was measured twice using a Glomax-Multi Detection System luminometer (Promega) and the Dual-Luciferase Reporter Assay (Promega ...
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bioRxiv - Molecular Biology 2019Quote: ATP was assessed by using a luminescence based ATP detection assay according to the manufacturer’s instructions (CellTiterGlo3D, G9681, Promega). ATP standards were prepared from a 10mM stock solution (P1132 ...
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bioRxiv - Immunology 2020Quote: ... Firefly luciferase activity was then measured with BrightGlo luciferase reagent and a Glomax-Multi+ Detection System (Promega, Southampton, UK). Pseudovirus neutralization titres were expressed as the reciprocal of the serum dilution that inhibited luciferase expression by 50% (IC50).
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bioRxiv - Immunology 2021Quote: ... Relative levels of IFNλs were estimated using the extracellular HiBiT split luciferase assay by virtue of their C-terminal HiBiT tag by incubating IFN preparations with assay reagents and measured by manufacturer’s instructions (Nano-Glo HiBiT Extracellular Detection system, Promega) using a luminometer.
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bioRxiv - Immunology 2020Quote: ... Firefly luciferase activity was then measured with BrightGlo luciferase reagent and a Glomax-Multi+ Detection System (Promega, Southampton, UK). Pseudovirus neutralization titres were expressed as the reciprocal of the serum dilution that inhibited luciferase expression by 50% (IC50).
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bioRxiv - Microbiology 2023Quote: ... the HiBiT value was measured 2 days after transfection using the Nano Glo HiBiT Lytic Detection System (N3040, Promega) as described previously51 ...
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bioRxiv - Neuroscience 2023Quote: Glucose levels in culture media were quantified using a bioluminescent NADH detection assay according to manufacturer’s instructions (Glucose-glo, Promega). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cell surface levels of HiBiT-tagged human S1PR1 were monitored using a Nano Glo HiBiT Extracellular Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... and heterozygous and homozygous clones were identified and screened using the Nano-Glo HiBiT Lytic Detection System (Promega #N3030). We confirmed in-frame insertions by PCR ...
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bioRxiv - Immunology 2024Quote: ... Firefly luciferase activity was measured after replacing the media with 50µl Bright-Glo luciferase reagent and measuring luminescence on a GloMax-MultiTm Detection system (Promega). Neutralization titers were calculated by interpolating the reciprocal serum dilution at which there was a 50% inhibition of luciferase activity relative to untreated controls (ND50).
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bioRxiv - Cancer Biology 2024Quote: ... Authenticity of cell lines was evaluated by detection of ten genetic loci using the GenePrint® 10 System (Promega) and cross referencing to ATCC or internal genetic profiles.
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bioRxiv - Microbiology 2020Quote: ... Two hundred nanograms of purified RNA were reverse-transcribed using Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT, Promega). RT was primed with oligo dT(15 ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was detected using the CDC 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel as per Centre for Disease and Control’s optimised protocol (Integrated DNA Technologies) for use with Go-Taq 1-step RT-qPCR Master Mix (Promega). Gene expression profile and SARS-CoV-2 N subgenomic RNA expression were determined with a standard qPCR cycle (50°C for 2 minutes ...