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Citations for Promega :
951 - 1000 of 1897 citations for Recombinant Human CLEC11A Protein T7 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Proteins were digested overnight with sequencing-grade modified trypsin (Promega). Then ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C with trypsin (Promega) and Lys-C (Wako,125-05061 ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were digested with 6 ng/µL trypsin (Promega, UK) overnight at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... proteins in the plugs were digested overnight with trypsin (Promega) at 37°C in a 25-mM NH4HCO3 buffer (0.2 µg trypsin in 20 µL) ...
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bioRxiv - Plant Biology 2023Quote: ... alkylation (55 mM CAA) and protein digestion (trypsin-gold, Promega) the resulting peptides were purified using self-packed StageTips (C18 material ...
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bioRxiv - Physiology 2024Quote: ... Proteins were digested with 1 µg trypsin (sequencing grade, Promega) overnight at 37°C under gentle agitation ...
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bioRxiv - Cancer Biology 2024Quote: ... The proteins were then digested by trypsin (sequencing-grade, Promega) at 1:50 ratio at 37 °C overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... and digested according to the In-solution Protein Digestion (Promega) protocol ...
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bioRxiv - Molecular Biology 2020Quote: Human Genomic DNA was extracted from the blood using the Wizard Genomic DNA Purification kit (Promega) as per the instructions.
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bioRxiv - Immunology 2021Quote: ... 25 μl of 7.5×104 Jurkat effector cells expressing either human FcγRIIIa or murine FcγRIV (Promega) resuspended in assay buffer were added to each well ...
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bioRxiv - Molecular Biology 2021Quote: Full-length human RNU6 sequence (110 nucleotides) was amplified by PCR and cloned into psiCHECK2 (Promega), downstream of Renilla luciferase ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA (gDNA) was extracted from human frontal cortex using Wizard Genomic DNA Purification Kit (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Standards for quantification were created by serial dilution of pre-quantified BSC human male DNA (Promega). Amplification reactions were performed in duplicate using 96 well-plates using a CFX96 Touch Real-time PCR Detection System (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: The region of interest (750-850 bp) was PCR amplified from pooled male human DNA (Promega) and cloned into a STARRseq luciferase validation vector_ORI_empty plasmid (Addgene plasmid #99298 ...
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bioRxiv - Molecular Biology 2020Quote: ... WT or mutant promoter of human Rpl28 gene was cloned into pGL-3-basic (Promega, E1751). The number is relative to transcriptional start site ...
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bioRxiv - Bioengineering 2020Quote: ... and detected with anti-rabbit or anti-human HRP-conjugated secondary antibodies (Promega W4011 and W4031). Blots were developed using ECL (ThermoScientific 32106 ...
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bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
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bioRxiv - Immunology 2023Quote: ... 100 μl of 5,000-fold diluted Peroxidase AffiniPure goat anti-human IgG (H+L) antibody (Promega) was added into each well and incubated for 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Human and murine NLRC5 and CXCL10 promoters were cloned into pGL3 basic luciferase reporter vector (Promega). All mutated plasmids were constructed from wild type plasmids by Fast Mutagenesis System (TransGen Biotech) ...
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bioRxiv - Molecular Biology 2019Quote: ... for 30 minutes at room temperature and then digested by LysC (Lysyl endopeptidase, #125-05061, Wako, ratio LysC/protein 1:100) and trypsin (Trypsin Gold, V5280, Promega, ratio trypsin/protein 1:20) overnight ...
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bioRxiv - Cancer Biology 2021Quote: Each enhancer or promoter region was amplified from human genomic DNA and cloned into pGL4.10 [luc2] (Promega) containing a SNP (rs718960 ...
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bioRxiv - Neuroscience 2020Quote: ... and pCMVΔ8.9 plasmids were co-introduced into human embryonic kidney 293T cells using the FuGENE reagent (Promega). 48 h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... albicans to damage human vascular endothelial cells was assessed by the CytoTox-96 assay (Promega, Madison, WI), which measures the release of lactate dehydrogenase (LDH ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were transfected with human RXFP1 pcDNA-Zeo-tetO and the GloSensor reporter plasmid using FuGENE (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... HEK 293T cells were co-transfected with human MOR and a luciferase-based cAMP biosensor (GloSensorTM, Promega). The next day ...
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bioRxiv - Genetics 2022Quote: We performed transient transfection on human pluripotent stem cells using FuGENE® HD Transfection Reagent (Promega, E2311). The cells were splitted and treated with Y-27632 for one before transfection.
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bioRxiv - Cell Biology 2024Quote: All human cell lines were authenticated at each batch freezing by STR profiling (StemElite ID System, Promega). All cell lines were tested for mycoplasma at each batch freezing by PCR (32 ...
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bioRxiv - Genomics 2023Quote: ... Primer efficiency was calculated using a 10-fold serial dilution of Human Mixed Genomic DNA (Promega G3041). For primer sequences and calculated efficiencies ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins in the excised band were digested with Trypsin (Promega, V5280). The resulting peptides were analyzed online by nano-flow HPLC-nanoelectrospray ionization using a Qexactive HFX mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: TNT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) was used to perform in vitro translation assay ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were digested with 10ng/ul of sequencing grade trypsin (Promega) overnight at 37C ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then digested using modified trypsin (Promega, P/N V5113) at a 1:20 (w/w ...
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bioRxiv - Immunology 2019Quote: ... Proteins were digested with sequencing grade trypsin (1:100; Promega, USA) overnight at 37°C and peptides concentration was checked by spectrophotometry (Nanodrop 2000 ...
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bioRxiv - Microbiology 2021Quote: ... Crosslinked proteins were digested by the addition of 1μg trypsin (Promega) and overnight incubation at 30°C ...
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bioRxiv - Neuroscience 2020Quote: ... The protein digestion was performed by adding 0.2 µg LysC (Promega) in 50 µL of 100 mM ABC for 3h at 1200 rpm at 37°C followed by overnight trypsin digestion using 0.2 µg of trypsin (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... Fusion proteins were expressed by TnT® Quick Coupled system (Promega). 15 μL of HA tagged protein was then incubated with candidate GST-coated beads at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Protein digestion was carried out with sequencing grade modified Trypsin (Promega) at 1/50 protease/protein (wt/wt ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were digested by addition of sequencing-grade trypsin (Promega) at an enzyme-to-substrate ratio of 1:25 ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were digested with 500 ng trypsin (Promega, Madison, USA) overnight at 37°C on a shaking incubator ...
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bioRxiv - Plant Biology 2021Quote: ... Expressed proteins were directly captured using Magne Halo Tag Beads (Promega). The protein-bound beads were incubated with adaptor-ligated gDNA fragments ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were on-bead digested with 1 µg trypsin (Promega) for 4 hours at 37 °C under agitation ...
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bioRxiv - Cell Biology 2021Quote: ... Then the protein was digested with sequencing grade modified trypsin (Promega) (1 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were then digested with 12.5 ng/uL modified trypsin (Promega) in 50 μL 100 mM ammonium bicarbonate ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described (Casabona et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested with Trypsin/ Lys-C (Promega, WI, USA). According to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... anti-GFP (Abkine A02020) and Magnetic Protein G Beads (Promega, G747A) overnight at 4°C with gentle agitation ...
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bioRxiv - Systems Biology 2022Quote: ... Proteins were digested with mass spec grade Trypsin/LysC mix (Promega) at a 1:50 (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples were mixed with Trypsin Gold (Promega, Madison, WI, USA) at a 50:1 ratio (w/w ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested with trypsin overnight (0.12 μg Trypsin Gold [Promega UK Ltd ...
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bioRxiv - Plant Biology 2021Quote: ... Enzymatic digestion of proteins using LysC/Trypsin Mix (Promega, Fitchburg, WI) was then performed according to the technical manual ...