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Citations for Promega :
951 - 1000 of 1250 citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The sample solutions were diluted to a final concentration of 2 M urea and 50 mM ammonium bicarbonate prior to the trypsin (Promega) digestion ...
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bioRxiv - Cell Biology 2024Quote: ... Bacmid DNA was extracted from overnight cultures using isopropanol precipitation as described.72 About 1 × 106 Sf9 cells in 2 mL of media in a 6-well dish were transfected with up to 2 μg of fresh bacmid DNA using FuGene HD transfection reagent (Promega) at a ratio of 3:1 (Fugene reagent:DNA ...
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bioRxiv - Microbiology 2024Quote: In vitro toxicity was assessed in HeLa cells (ATCC® CCL-2) using the ApoTox-GloTM Triplex Assay (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The 3’UTR of human Akt or Pin1 was amplified by PCR from fibroblast genomic DNA and cloned downstream of the Renilla luciferase gene in the psiCHECK-2 dual reporter construct (Promega) by XhoI (Akt ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted by 4-fold to reduce urea to 2 M for the addition of trypsin (Promega, 1:50 w/w) to continue the digestion at 21 °C overnight ...
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bioRxiv - Molecular Biology 2024Quote: Luciferase PRE reporters were generated by inserting PRE repeats into the 3’UTR of Renilla luciferase in the psiCheck-2 dual luciferase reporter vector (Promega) (Table S7) ...
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bioRxiv - Biochemistry 2024Quote: ... elution buffer I (2 M Urea, 50 mM Tris pH 7.5, 1 mM DTT and 5 ng/µl Trypsin (Promega, #V5111)) were added and samples were incubated for 1 h at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... Transfection for each well was performed with a mixture of 2 µg of DNA and 6 µL of Fugene HD reagent (Promega). The DNA/fugene mixture was incubated for 15 minutes at room temperature prior to its drop-wise addition to the infected culture ...
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bioRxiv - Molecular Biology 2024Quote: ... then media changed to 100 µL of equilibration media consisting of Ca2+- and Mg2+-free HBSS containing 2% (v/v) dilution of the GloSensor cAMP Reagent stock solution (Promega). Cells were incubated for 2 h at 37°C ...
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bioRxiv - Immunology 2024Quote: ... The 50% tissue-culture infectious dose (TCID50) of SARS-CoV-2 pseudovirus was determined using the Steady-Glo Luciferase assay system (Promega).
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bioRxiv - Microbiology 2024Quote: The upstream regions of the ampC and cphA genes were amplified by PCR (Supplementary Table 2) and cloned into the pGEM-T easy plasmid (Promega). The inserts were subcloned into the pRKlacZ290 vector to obtain transcriptional fusions of the lacZ gene ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR fragment was directionally cloned downstream of the Renilla luciferase ORF in the psiCHECK-2 vector (Promega, Madison, US) using the Quick Ligase kit (M2200 ...
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bioRxiv - Cell Biology 2024Quote: ... 50 μL IVT reactions were carried out for 2 hours at 30°C using commercially available Nuclease-Treated Rabbit Reticulocyte Lysate (RRL, Promega) and reaction components were added in the following order ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... The annealed oligos were ligated into the Xho I and Not I sites in the 3’UTR of the Renilla luciferase gene in the psiCHECK-2 plasmid (C8021, Promega). Each plasmid was transfected individually into HEK 293 cells with miR-891b using DharmaFECT 1 Transfection Reagent (T-2001-03 ...
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bioRxiv - Molecular Biology 2024Quote: 3′-RACE assays were conducted using 2 μg of total RNA extracted from HEK293 cells using an RNA isolation kit (Promega) following established protocols (Shaji ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were then digested with 1 μg of Lysyl-endopeptidase (Wako) at room temperature for 2 hours and further digested overnight with 1:50 (w/w) trypsin (Promega) at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... RLI from transduced cells was added to cytokine-starved CTLL-2 cells with proliferation of these cells subsequently measured via Colorimetric MTS Assay (Promega). Co-culture assays were conducted by incubating SB28 tumor cells with freshly isolated NK cells (BioLegend MojoSort™ NK Cell Isolation Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... Cellular proliferation was also measured at Day 0 and Day 2 using RealTime-Glo™ MT Cell Viability Assay (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... followed by a secondary anti-rabbit IgG antibody conjugated to alkaline-phosphatase (Promega, Madison, WI, USA). The membranes were blocked in TBST (150 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... followed by the incubation with a secondary antibody anti-mouse IgG HRP conjugate (W4021, Promega, U.S.A.). All the primary antibodies were diluted 1/3000 in 10% Blocking One/TTBS (50 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: Primary antibodies and the dilutions used are as follows: anti-Halo (mouse, Promega G9211, 1:200); anti-Ezh2 (mouse ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 1:1000 dilution and a secondary antibody against rabbit coupled to HRP (Promega W4011) at a 1:20 000 dilution.
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bioRxiv - Microbiology 2021Quote: ... immunoblots were probed with the appropriate secondary α-mouse antibody conjugated to horseradish peroxidase (Promega, W402B) at a dilution of 1:5000 ...
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bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega. Chemiluminescence images were acquired using the ImageQuant LAS 500 system (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... were used as secondary antibodies and visualized by detecting chemiluminescence (32209, Pierce) or fluorescence (S1000, Promega) signals ...
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bioRxiv - Bioengineering 2020Quote: ... and detected with anti-rabbit or anti-human HRP-conjugated secondary antibodies (Promega W4011 and W4031). Blots were developed using ECL (ThermoScientific 32106 ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies used were: rabbit and mouse anti-βGal (1/1000; Promega and MP Biomedicals, respectiveley), rat anti-Ser (1/1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... and then with 1:5000 of anti-rabbit (#W401B) or anti-mouse (#W402B) secondary antibodies (Promega).
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bioRxiv - Cancer Biology 2021Quote: Antibody-dependent cell-mediated cytotoxicity (ADCC) was assessed by an ADCC Reporter Bioassay (Promega, Madison, WI) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary horseradish peroxidase-conjugated anti-mouse or anti-rabbit rabbit IgG antibody (Promega #W4021 and #W4011) were used at 1:2,500 dilution followed by detection by ECL (SuperSignal West Pico Chemiluminescent Substrate ...
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bioRxiv - Cell Biology 2022Quote: ... Subsequently anti-rabbit IgG horseradish peroxidase (HRP)-conjugated secondary antibody (Cat. No. W4011, Promega, Madison, WI) was used to detect the protein of interest with chemiluminescence reaction ...
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bioRxiv - Cell Biology 2022Quote: ... washed and incubated with secondary antibody goat-anti-rabbit-HRP or goat-anti- mouse-HRP (Promega) for 1 h ...
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bioRxiv - Genomics 2022Quote: ... Sections were then incubated with a horseradish peroxidase-labeled anti-rabbit antibody (1:300, Promega, W401B) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged or HA-tagged protein was detected using a commercially available mouse monoclonal antibody (Promega).
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bioRxiv - Immunology 2023Quote: ... 100 μl of 5,000-fold diluted Peroxidase AffiniPure goat anti-human IgG (H+L) antibody (Promega) was added into each well and incubated for 1 h at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega. Chemiluminescence images were acquired using the ImageQuant LAS 500 or 600 system (GE Healthcare).
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bioRxiv - Genetics 2023Quote: ... and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP, Promega). In order to detect the endogenous Ptiwi09 protein ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated for another hour with an anti-Mouse IgG HRP-conjugate secondary antibody (Promega) (diluted 1:10 000 in PBST) ...
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bioRxiv - Immunology 2024Quote: Antibody dependent cell cytotoxicity was measured using a commercially available LDH Non-Radiactive Cytotoxicity Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... the membrane was incubated with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted 1:10.000 in PBS-T with 3% milk powder for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Culture media was then replaced with 50 µL of qNSC/aNSC media containing 1 mM 2DG (2-deoxy-D-Glucose, provided in Glucose Uptake-Glo kit from Promega (J1342)) reagent for 10 minutes in incubator (humidified ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and then diluted 2-fold with 200 mM ammonium bicarbonate for trypsin digestion (1:10 w:w, 37°C, 8h, Promega cat # V5113).
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bioRxiv - Physiology 2020Quote: ... and single-stranded cDNA was synthesized from each sample (2 μg) with M-MLV reverse transcriptase (#0000113467, Promega Corporation, Fitchburg, WI) and RNase inhibitor (40 U ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye reduction assay as described previously [20] or by CellTiterGlo assay (Promega, Walldorf, Germany) following the manufacturer’s instructions after 120h incubation.
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bioRxiv - Immunology 2021Quote: ... Products were isolated from a 2% agarose gel using the Wizard1 SV Gel and PCR Clean-Up System (Promega, Mannheim, Germany). DNA concentration was determined via the Qubit1 1.0 Fluorometer (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: We performed the apoptosis analysis of the MPro stable cells and cells transiently transfected with the pLVX-MPro-eGFP-2 plasmid using the RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay kit from Promega. The cells were maintained in high glucose DMEM medium supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... and then diluted 2-fold with 200 mM ammonium bicarbonate for trypsin digestion (1:10 w:w, 37°C, 8h, Promega cat # V5113). After digestion ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Cancer Biology 2022Quote: ... The RSB/RNasin buffer (2 ml) was prepared fresh and contained 200 ul of 10 X RSB and 50 ul RNasin (N2511, Promega, USA). The PEB buffer (10 ml ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR (qPCR) reactions were conducted in a 10-µL total volume using a 2× GoTaq® qPCR Master Mix (Promega) and run on an ABI 7500 qPCR thermocycler (Applied Biosystems) ...