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Citations for Promega :
951 - 1000 of 3605 citations for 5 NAPHTHALEN 1 YL 2H PYRAZOL 3 YLAMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were co-transfected with 5’ SORL1 proximal promoter fragments in pGL4.10[luc2] vector (Promega, E6651) and pRL-TK Renilla control reporter vector (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were incubated at 37 °C overnight and subsequently treated with 5 units of DNase (Promega) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... In-gel digestion was performed using 5 ng/μL mass spectrometry-grade trypsin (Trypsin Gold, Promega) in 50 mM NH4HCO3 digestion buffer ...
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bioRxiv - Physiology 2019Quote: ... USA) and digestion with 150 ng trypsin (5 h, 37 °C Promega, Sequencing Grade, Wisconsin, USA) using a Tecan Freedom EVO robotic liquid handler (Tecan Systems ...
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bioRxiv - Microbiology 2019Quote: ... and remaining tissue incubated in 1X HBSS (without Ca2+ and Mg2+) containing 5 mM EDTA (Promega) and 1 mM DL-Dithiothreitol (DTT ...
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bioRxiv - Immunology 2019Quote: ... and the reaction volume was topped up to 5 μL with RNase/DNase-free H2O (Promega). Thermal cycling was carried out using a Viia7 real time PCR system (Applied Biosystems) ...
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bioRxiv - Genetics 2019Quote: ... The 5 kb CcMoY fragment was then cloned in the pGEM®-T Easy vector (Promega) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... PCR reaction mixture was formulated using 5 μl GoTaq G2 green PCR master-mix (Promega, USA), 1 μl primer (forward and revers ...
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bioRxiv - Developmental Biology 2020Quote: Tryptic digestion of bead-bound protein was performed in 5% w/w trypsin (Promega, cat. #V5280), 50mM triethylammonium bicarbonate buffer at 37 °C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Fugene HD (Fugene HD [μL]:DNA [μg]:DMEM [μL] = 5:2:200;Promega, Madison, WI, USA) was used for transient transfection ...
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bioRxiv - Neuroscience 2021Quote: ... then 50 µL of drug buffer with RLuc substrate coelenterazine h (Promega, 5 µM final concentration) was added per well ...
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bioRxiv - Immunology 2021Quote: ... Infection was measured after 5 days at 37°C by the Luciferase assay system (Promega, USA) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... the PkHSP70 5’UTR sequence was amplified and inserted into a pGem-T Easy vector (Promega) followed by the Cre60 sequence via restriction cloning between XhoI/KpnI sites ...
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bioRxiv - Systems Biology 2022Quote: ... In-solution digestions were performed on lysates containing 5 mg of protein using either Trypsin (Promega), Chymotrypsin (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... Syp-Halo protein was incubated with 5 times molar excess halo ligand Alexa 488 (Promega, USA) overnight at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... Half of the lysis was treated with 5 µl of 10 mg/ml Rnase (Promega:527491) and both lystates (+/- RNase ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at 10 μM for CSNK2A1 or 5 μM for CSNK2A2 in Tracer Dilution Buffer (Promega N291B) was added to all wells ...
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bioRxiv - Microbiology 2023Quote: ... 5% CO2 before measurement of luciferase activity using Nano-Glo® Luciferase Assay (Promega, Cat # N1110) and Tecan Spark plate reader.
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bioRxiv - Immunology 2022Quote: ... Cells were then washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in cell lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... The PeSPL6 and PeSPL13a 5’-ends were amplified by PCR using the GoTaq Master mix (Promega), the forward 5’ Primer from GeneRacer kit and the reverse Gene Specific Primer (GSP) ...
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bioRxiv - Genomics 2023Quote: ... Samples were then allowed to cool for 5 minutes and 5μl of RNase (DNase-free; Promega) was added and incubated at 37ºC for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Digests were diluted further to a 1.5 M urea concentration and 5 µg of trypsin (Promega) was added for 6 hours at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... gel plugs were crushed into small pieces and 5–10 μg of sequencing-grade trypsin (Promega) per ∼100 μg protein were added ...
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bioRxiv - Immunology 2021Quote: ... A 1:1 dilution of Steady Glo (Promega) and Lysis Buffer were added to the cells and incubated at RT for 15 mins ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-1-gal (1:1000, Promega Z378B), mouse anti-1-gal (1:1000 ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... 1 mM CaCl2 with 1 µg trypsin (Promega). Digested proteins were acidified with TFA to pH < 2 and RapiGest SF was precipitated out ...
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bioRxiv - Cancer Biology 2021Quote: MAGI2-AS3 or FOXN3 3’UTR containing miR-452-5p wild-type (WT) or mutant (MUT) binding sites were inserted into psiCHECK vector (Promega, USA). 293T cells were transfected with the constructed luciferase plasmid together with miR-NC or miR-452-5p mimics using Lipofectamine 2000 ...
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bioRxiv - Cell Biology 2020Quote: ... The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were transfected with 700 ng of plasmid construct (PIB/V5_Gr9) and 3 μl of FuGENE® HD transfection reagent (Promega, USA) in 100 μl of medium per well ...
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bioRxiv - Physiology 2022Quote: A DNA fragment corresponding to partial f5 or f3a cDNAs was amplified from ovarian cDNAs by PCR using the gene-specific primers (see Supplemental Table 3 for detail) (28) and cloned into the pGEM-T Easy vector (Promega, USA). Correct sequence and orientations of inserts were validated by Sanger sequencing ...
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bioRxiv - Microbiology 2021Quote: ... Remaining ATP was readout after 3 h incubation using Kinase-Glo® Luminescent Kinase kit following manufacturer’s instructions (Promega, WI, USA).
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bioRxiv - Cell Biology 2021Quote: ... of miR-148a putative binding sites reporter plasmids were constructed using the circMRPS35 and 3’-UTR of STX3 sequences in the psiCHECK2 vector (Promega, USA). For IRES activity analysis ...
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bioRxiv - Cell Biology 2020Quote: Transfections were carried out with 1 μg of total DNA (500 ng for each construct or with empty expression plasmid) and 3 μl of FuGENE ® HD Transfection Reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested from Calu-3 cells and inactivated by addition of lysis buffer (Maxwell 16 viral total nucleic acid purification kit, Promega #AS1150) complemented with proteinase K ...
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bioRxiv - Microbiology 2021Quote: The cell viability of Vero E6 and Calu-3 cells was analyzed by quantification of ATP levels using the CellTiter-Glo™ Luminescent Cell Viability assay (Promega) according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: Cell death by apoptosis was measured using Caspase-Glo 3/7 luminescent assay system kit according to the manufacturer’s instructions (Promega Madison, WI). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... the coding sequence of RE02 extended by BsaI cleavage sites (Supplementary Table 3) was cloned into the pGEM®-T vector (Promega). The cauliflower mosaic virus 35 promoter ...
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bioRxiv - Microbiology 2022Quote: Activity of caspases-3/7 and -8 were assessed using the corresponding Caspase-Glo® Assays in white-walled 96-well plates (Promega).
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bioRxiv - Biochemistry 2023Quote: 10 μL in vitro nLuc mRNA translation reactions were performed in the dynamic linear range using 3 nM mRNA (31) in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 20% RRL ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were generated with pCS2-N-terminal 5×MYC vectors and pCS2-N-terminal 3×HA vectors described above using TnT® Coupled Reticulocyte Lysate System under the SP6 promoter (L4600, Promega) and by following manufacturer’s recommendations with few modifications ...
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bioRxiv - Molecular Biology 2023Quote: 10 μL in vitro translation reactions were performed in the linear range using 3 nM mRNA in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 30% RRL ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... drugs were added to plates in triplicate and incubated for 3 days before being developed by adding CellTiterGlo (Promega, Madison, WI) per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: The short TMEM106B 3’ UTR and the long TMEM106B 3’ UTR were amplified from human genomic DNA (H1) and then cloned into the pmirGLO Dual-Luciferase Vector (Promega, E1330) using Gibson assembly ...
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bioRxiv - Cancer Biology 2024Quote: The apoptotic effect of MK-1775 was determined by means of caspase 3/7 activity via Apotox-Glo Triplex Assay (Promega, #G6320) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR amplification was performed in 25 μl reactions using 5 μl X Green GoTaq Flexi Buffer (Promega), 1.5 μl MgCl2 (25mM ...
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bioRxiv - Cancer Biology 2019Quote: ... Marinnisen and the pG5 Luc (minimum promoter containing 5 sites for GAL4 binding) was purchased from Promega. The expression plasmid for Nrf2 WT ...
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bioRxiv - Biophysics 2020Quote: ... samples from homozygous HaloTag-TEV mice were incubated overnight with 5 μM HaloTag Oregon Green ligand (Promega) in Phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was digested by incubation overnight at 37°C with trypsin (5 ng/ ml, Promega, Madison, WI) in 50 mM NH4HCO3 (pH 7.8) ...
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bioRxiv - Immunology 2019Quote: ... 5% CO2 for another 24 h before 20 μL of CellTiter 96 Aqueous One Solution (Promega, #G3582) was added and absorbance at 490 nm and 620 nm was measured ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated with 5 mM iodoacetamide for 30 min in the dark and digested overnight with trypsin (Promega; 1 μg for cell-surface-enrichment samples ...