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Citations for Promega :
951 - 1000 of 3609 citations for 4 2 Methylimidazol 1 ylmethyl phenylamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Samples were then diluted 1:1 with Milli-Q water and trypsin (Promega) added at the same enzyme/protein ratio ...
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bioRxiv - Cell Biology 2020Quote: ... psPAX2 and pMD2.G at the ratio of 1:1:1 in HEK293T cells using ProFection® Mammalian Transfection System (Promega, E1200), medium was changed 16h post transfection and virus containing supernatant was harvested 48h later ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was diluted 1:1 with 50 mM ammonium bicarbonate and proteins were digested with trypsin (protein to enzyme ratio 1:50, Promega, Mannheim, Germany) overnight at 37°C ...
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bioRxiv - Genomics 2022Quote: ... The samples were diluted 1:1 with milliQ water and were incubated with a 1:100 w/w amount of trypsin (Promega, sequencing grade) overnight at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... The kinase activity assay was performed with 4 µl beads in a 384-well plate using the ADP-Glo™ Kinase Assay (Promega, Madison, WI). The luminescence of each well was read using a Synergy™ H1 Hybrid Multi-Mode Microplate Reader (BioTek Instruments ...
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bioRxiv - Microbiology 2020Quote: ... collected by centrifugation (17,000 g for 10 min at 4°C) and solubilized in 20 μl 50 mM TEAB containing 0.2 % ProteaseMAXTM Surfactant (Promega UK Ltd, Cat. # V2071) for 1-2 h with vortex and occasional sonication ...
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bioRxiv - Cell Biology 2020Quote: ... washed again in DPBS and stained with 1mg/ml X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside, Promega, Madison, WI, US) in DMF (Dimetil formamide ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... PHH were infected with HepAD38-derived GTD virus at 500 viral genome equivalents per cell in maintenance medium supplemented with 4 % PEG 8000 (Promega, Madison, WI; V3011). For patient sera infections ...
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bioRxiv - Microbiology 2022Quote: ... then dipped in a well of a 96-well PCR plate filled with 20 µl of a gyrB PCR mix (4 µl of 5x GoTaq buffer (Promega, Madison, WI, USA), 0.4 µl of 10mM dNTP ...
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bioRxiv - Microbiology 2023Quote: ... were not screened for Wolbachia.All PCRs were performed in a final reaction volume of 20 μl including 4 μl of 5X reaction buffer (Promega, Madison, WI, USA), 1.5 μl of dNTPs (2.5 mM) ...
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bioRxiv - Microbiology 2023Quote: ... and the reaction was revealed with a solution of NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate) (Promega, Madison, WI, USA).
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bioRxiv - Genetics 2024Quote: ... DNA was isolated from a variety of tissues that were collected from 4- and 8-month-old male mice using a Maxwell® 16 Mouse Tail DNA Purification kit (Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... First-strand cDNA was synthesized from 2 µg of total RNA using random hexamers and MMLV reverse transcriptase (Promega). The cDNA was used as a template for quantitative PCR performed using Syber Green reagent (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... and SREBP-2 (NM_004599) expression plasmids were prepared by subcloning PCR products into a mammalian expression plasmid pCI (Promega). Likewise ...
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bioRxiv - Molecular Biology 2021Quote: ... before cells were permeabilized using 0.2% Triton X-100 PBS for 10 min at room temperature, then blocked for 30 min (2% BSA / 0.5% fish skin gelatine / PBST.5, 2U/µL RNAsin Plus (Promega) at room temperature) ...
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bioRxiv - Genetics 2021Quote: ... 2-3 μl of the clear part of the solution was used for PCR with either Gotaq (Promega, M7808) or LongAmp 2X (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... the samples were brought to room temperature for 5-10 minutes while 2 mL of 30 mM coelenterazine (Promega), or 15 mL of 1-to-50 diluted NanoLuc substrate (Promega) ...
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bioRxiv - Bioengineering 2020Quote: ... the Nbs-virus mixture was removed from Vero E6 cells followed by 500 μL of DMEM with 2% FBS and 0.9% carboxymethyl cellulose (Promega) was overlaid ...
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bioRxiv - Genomics 2021Quote: ... Variant or variant haplotype fragments were amplified in African American heterozygous individuals carrying the variants of interest located in the middle of the fragments (Supplementary Table 2 and 3) and cloned into the enhancer reporter vector pGL4.24 (E8421, Promega). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 spike protein (23) into HEK 293T cells using the FuGENE 6 Transfection Reagent (Promega). Spike sequences from the following global strains REF were used ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was synthesized using 5μg of total RNA at 42 °C for 2 hours using avian myeloblastosis virus (AMV) reverse transcriptase (Promega). A 20 µL reaction containing 10 ng or 10 pg (for 16S rRNA ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was inserted into the multiple cloning site (MCS) of the psicheck-2 vector (supplied by Promega) downstream of the Renilla luciferase gene according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... alkylated using 50 mM iodoacetamide for 2 hrs at ambient temperature and then digested with 1.2 μg proteomics-grade trypsin/LysC (Promega) according to the SP3 protocol (27) ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected into HeLa cells for 24 h (2 μg of cDNA/9.6 cm2 plate) using Fugene (Promega). For gene silencing ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were diluted to a final concentration of 500,000 cells/mL in Expi293 expression medium plus 2% (v/v) Glosensor reagent (Promega) and incubated for 75 minutes at room temperature with gentle rotation ...
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bioRxiv - Genomics 2022Quote: Cell viability upon siRNA transfection was measured through the CellTiter 96® Non-Radioactive Cell Proliferation Assay (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT)) (Promega). 1× 104 microglia cells were plated per well in a 96 well plate and transfected with either ON-TARGETplus Non-targeting Control siRNA (Horizon Discovery) ...
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bioRxiv - Microbiology 2022Quote: ... Samples were digested by adding 100 µL trypsin solution (10 mM TEAB, pH 7.5, 2 µg Promega trypsin #V5111) and incubated overnight at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... while the complementary sequence was cloned downstream of the Renilla luciferase reporter cassette in a psiCHECK-2 plasmid (Promega). The same target sequence was also cloned downstream of a TagRFP reporter cassette in an ePB-BSD-TT piggyback vector (see plasmids ...
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bioRxiv - Plant Biology 2021Quote: ... benthamiana leaves were harvested 2-days after infection and then applied to a Dual-Luciferase Reporter Assay System (Promega) to detect the activation of the ACS2 and ACS2mut promoters by NOR ...
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bioRxiv - Neuroscience 2020Quote: ... The first strand cDNA was synthesized from 2 μg RNA with GoScript™ Reverse Transcriptase (Promega, Madison, WI, USA).
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bioRxiv - Cell Biology 2021Quote: ... and a total amount of 2 μg RNA was reverse transcribed with a GO Script Reverse Transcriptase kit (Promega) using random primers ...
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bioRxiv - Cell Biology 2022Quote: ... 2 hours post-transfection media was replaced with fresh complete DMEM containing 646 HALO Dye (20 nM; GA112A; Promega). The next day ...
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bioRxiv - Genomics 2022Quote: ... 2 µl of yeast lysate was used as a template in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Neuroscience 2022Quote: ... or 1.0 μg/side of MEK inhibitor 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]butadiene (U0126; Promega, Madison, WI); 0.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcriptase reaction was performed from 2 µg of total RNA by using ImProm-IITM Reverse Transcription System (Promega). Aliquots of cDNA obtained were amplified in PCR and quantitative real-time PCR reactions ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 ml (approximately 2 × 109 cells) were used for gDNA extraction with the Wizard Genomic DNA Purification kit (Promega), which typically yielded 50 μl of 0.5-1.5 μg/μl gDNA.
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bioRxiv - Microbiology 2020Quote: ... The plate was incubated in the dark for 2 mins then read on a Glomax Multi+ Detection System (Promega) as above ...
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bioRxiv - Microbiology 2020Quote: ... The plate was incubated in the dark for 2 mins then read on a Glomax Multi+ Detection System (Promega) as above ...
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bioRxiv - Physiology 2020Quote: ... Blocked membranes were incubated for 2 hours with DNA probe synthesized using a Prime-a-Gene® kit (Promega) and washed in mild and stringent Southern wash buffers ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were then incubated at 37 °C for 2 min before the addition of 30 units of SmaI (Promega), 46 kBq [a-32P]-dCTP (111 TBq/mmol ...
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bioRxiv - Cancer Biology 2021Quote: ... embryos were equilibrated (2 times 10 minutes) in NTMT buffer (NaCl 100mM, TrisHCl 100mM pH9,5, MgCl2 50mM, 2%Tween20) and incubated in NBT/BCIP (Promega) at RT in the dark until color development ...
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bioRxiv - Immunology 2020Quote: ... The following day the cells were transfected with plasmids encoding the indicated FLAG-tagged protein (2 μg) with FuGENE HD according to manufacturer’s instructions (Promega). After 48 h cells were harvested and washed 3 times with PBS ...
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bioRxiv - Immunology 2020Quote: ... The following day cells were transfected with plasmids encoding for FLAG-tagged NLRP1 (2 μg) with FuGENE HD according to manufacturer’s instructions (Promega). After 48 h ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected with 2 μg of fresh bacmid DNA and 6 μL of FuGene HD transfection reagent (Promega, E2311) according to the manufacture’s instruction ...
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bioRxiv - Plant Biology 2022Quote: ... on-bead digestion was performed after reduction with 10 mM tris(2-carboxyethyl)phosphine and alkylation with 10mM Iodoacetamide in 50 mM triethylamonium bicarbonate TEAB containing 0.01% ProteaseMAX surfactant (Promega). A 500 ng aliquot of sequencing-grade trypsin (Promega ...
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bioRxiv - Microbiology 2022Quote: ... either with SARS-CoV-2 S and Jun or with ACE2 and Fos plus the pRL Renilla Luciferase (Promega) to normalize the signal ...
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bioRxiv - Neuroscience 2023Quote: ... for 3 hours at RT and then continued overnight with addition of 2 μg of trypsin (Promega, Cat# V5280), at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 25% confluent wells were transfected with 2 µg plasmid vector expressing SV40 antigen using Fugene Transfection Reagent (Promega) as described by manufacturer ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: 2′3′-cGAMP (0.2 μM) and dsDNA (2 μg/mL) was transfected into cells using FuGENE HD Transfection Reagent (Promega), whereas the transfection reagent without dsDNA or 2′3′-cGAMP was added as the control ...
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bioRxiv - Microbiology 2022Quote: ... Samples were digested for 2 hr at 25°C with constant agitation with a Trypsin/LysC mix (V5071, Promega), and overnight at 25°C with proteomics-grade porcine trypsin (T6567 ...