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Citations for Promega :
951 - 1000 of 4459 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... pCAG-Flag or pCMV-HA-N expression vectors using standard molecular cloning methods as described in our previous publications.30–32 The IFN-β luciferase reporter plasmid pGL3-IFN-β-Luc vector was constructed in our previous study.33,34 The IFNλ1 luciferase reporter plasmid pGL3-IFNλ1-Luc was constructed by inserting the 1000-bp promoter region of human IFNλ1 (nucleotides −1000 to +1, with the translation start site set as 1) into pGL3-Basic (Promega, USA) according to methods outlined in previous studies.13,34 The ISG luciferase reporter plasmid pISRE-Luc vector was purchased from Clontech (USA) ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Trypsin digestion was carried out overnight at 37 °C with 1:50 - 1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Purified AaEnc and BSA were individually buffer exchanged into TBS pH 7.5 and mixed in a 40:1:1 molar ratio with commercially purchased trypsin (Promega, USA) and chymotrypsin (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... 58 °C for 45 seconds and 72 °C for 1 minute (32 cycles) and 72 °C for 10 minutes (1 cycle) using GoTaq G2 DNA polymerase (Promega). Additionally ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the coding sequence of HIV-1 Nef (SF2 variant) was amplified by PCR and inserted into the plasmid pNLF-1-C (Promega) for expression of NanoLuc at the Nef C-terminus ...
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bioRxiv - Cancer Biology 2022Quote: ... Urea was added to a final concentration of 1.6 M in 50 mM ammonium bicarbonate and 1 mM calcium chloride prior to digestion with 1 µg sequencing grade porcine trypsin (Promega) for 16 h at 37°C (74) ...
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bioRxiv - Biochemistry 2024Quote: ... The binding of the Nbs to the coated protein was detected via their EPEA-tag using a 1:4000 CaptureSelectTM Biotin anti-C-tag conjugate (Thermo Fischer Scientific) in combination with 1:1000 Streptavidin Alkaline Phosphatase (Promega). Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP ...
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bioRxiv - Biochemistry 2023Quote: ... Zc3h18-3F-mAID mES cells were transfected with pB-MYC-mZC3H18x-BSD vectors along with a piggyBAC transposase expressing vector (pBase) in a 1:1 ratio using Viafect (Promega). Cell pools were selected with BSD for ∼ 7-10 days or until negative control cells no longer survived ...
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bioRxiv - Neuroscience 2024Quote: ... the bead and protein mixtures were washed twice with 80% ethyl alcohol and once with 100% ACN before being resuspended in 95 µL NH4HCO3 prior to overnight on-bead digestion (enzyme–protein ratio of 1:20) at 1 000 rpm at 37 °C using modified porcine trypsin/Lys-C Mix (Mass Spectrometry Grade, Promega). The digestion was stopped using trifluoroacetic acid (TFA ...
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bioRxiv - Immunology 2023Quote: The specific activity of Caspase-1 was assessed in cell-free supernatants using Caspase-Glo® 1 Inflammasome Assay (Promega). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... cellular ATP concentrations were measured by adding 20 μL of 1x CellTiterGlo 2.0 (CTG) solution (1 part PBS, 1 part CellTiterGlo2.0 stock solution; Promega Cat# G9241) using a MultidropTM Combi liquid dispenser and measuring the luminescence on a SpectraMax iD3 (Molecular Devices).
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bioRxiv - Biochemistry 2022Quote: ... The sample was combined with 69 μL of ammonium bicarbonate along with 1 μL of 1% ProteaseMax surfactant (Promega V2072) for a total volume of 100 μL ...
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bioRxiv - Biophysics 2022Quote: ... Then the samples were diluted to 1 M urea with 50 mM (NH4)HCO3 (AB) and trypsinized (1:50 m/m, Promega) at 37 °C (overnight ...
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bioRxiv - Immunology 2022Quote: ... plates were equilibrated at room temperature for at least 30 minutes before 100 µL of 1:1 diluted PBS and CellTiter Glo reagent (Promega) was added to 100 µL of media ...
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bioRxiv - Molecular Biology 2023Quote: Cell pellets were lysed in cell lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% Triton-X with protease inhibitor cocktail (Promega)) and subjected to Western blotting ...
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bioRxiv - Neuroscience 2023Quote: ... urea concentration for each sample was diluted to <1 M using ammonium bicarbonate (ABC) and digested for an additional ∼16 hours using 1:50 (w/w) trypsin (Promega). Following digestion ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were visualized on 1% agarose gels (90 V, 30-45 minutes) against a 1 kb DNA ladder (Promega). Sanger sequencing was performed by the sequencing unit at the Max Planck Institute for Evolutionary Biology ...
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bioRxiv - Cancer Biology 2024Quote: ... and then incubated overnight at 4°C with mouse anti-HiBiT antibody (Promega #N7200; 1 µg/mL; diluted 1:1000) in 1X PBS containing 5% BSA ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 hr incubation in a 1:10000 dilution of the secondary antibody (anti-mouse IgG HRP conjugate, W402B, Promega) at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 hr incubation in a 1:10000 dilution of the secondary antibody (anti-mouse IgG HRP conjugate, W402B, Promega) at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 50μL of clarified lysates were transferred to a 96-well plate (white, flat-bottom, SPL) and mixed (1:1) with BacTiter-Glo (Promega) reaction buffer followed by incubation at 37°C for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... into 96-well PCR plates with 1 μL of cell lysis solution (1:10 Cell Lysis buffer [Roche], 10 U/μL Rnasin plus Ribonuclease inhibitor [Promega]) in each well ...
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bioRxiv - Biochemistry 2024Quote: ... Purified Haspin (465-498; 250 nM) was incubated for 1 hour at room temperature following addition of 1 mM ATP (Promega) in a reaction volume of 50 µL (in 50 mM Tris-Cl pH 7.6 ...
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bioRxiv - Molecular Biology 2024Quote: ... Wildtype mES cells were transfected with pB[MYC-] or pB[MYC-mTurbo-PHAX] vectors along with a piggyBAC transposase expressing vector (pBase) in a 1:1 ratio using Viafect (Promega). Cell pools were selected for successful integration of pB plasmids with Blastocidin (BSD ...
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bioRxiv - Plant Biology 2024Quote: ... columns were pre-equilibrated with 5 CV of 0.1 M NH4CO3 and then loaded with 1 CV of 0.1 M NH4CO3 containing 1 µg/ml trypsin gold (Promega V5280, Promega, USA). The flow-through containing the digested peptides of S-nitrosated proteins (elution 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... and Caspase 3/7 Glo (Promega #G8091) were added and assayed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 3 μL of Cell Titer Glo (Promega) were added to each well ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: ... For the Caspase 3/7 Glo (Promega) assay ...
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bioRxiv - Biochemistry 2020Quote: ... and Caspase-Glo 3/7 assay (Promega), respectively ...
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bioRxiv - Zoology 2021Quote: ... and 3% 20mg/ml Proteinase K (Promega). This digestion eliminates cellular material while leaving the spongin network intact.
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bioRxiv - Cell Biology 2022Quote: ... We added 3 μg Trypsin Gold (Promega) to each sample for digestion and incubated samples at 37°C overnight (approximately 16 hours ...
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bioRxiv - Microbiology 2022Quote: ... 3 μL of dNTPs (20mM, Promega®), 5 μL of buffer (5x ...
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bioRxiv - Microbiology 2022Quote: ... 3 μL of dNTPs (20mM, Promega®), 5 μL of buffer (5x ...
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bioRxiv - Cancer Biology 2022Quote: ... The Caspase-Glo 3/7 Assay (Promega) was performed according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: A caspase - Glo 3/7 kit (Promega) was used to evaluate caspase 3/7 activity based on the kit protocol ...
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bioRxiv - Zoology 2020Quote: ... and 3% 20mg/ml Proteinase K (Promega). Many additional photos of spicules and sections are available in the supplementary data that accompanies this paper ...
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bioRxiv - Genetics 2020Quote: ... 3 μl of RNasin ribonuclease inhibitor (Promega) per ml of extraction buffer] ...
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bioRxiv - Microbiology 2024Quote: ... the Caspase-Glo-3/7 kit (Promega) was used as previously described (22) ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 μl MgCl 25 mM (Promega A3511), 0.2 mM dNTPs ...
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bioRxiv - Cancer Biology 2023Quote: ... by Caspase-Glo 3/7 assay (Promega), were assessed using manufacturer’s instructions ...
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bioRxiv - Zoology 2024Quote: ... and 3% 20mg/ml Proteinase K (Promega). This digestion eliminates cellular material while leaving the spongin network intact ...
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bioRxiv - Cell Biology 2023Quote: ... 3 ul of RNasin Ribonuclease Inhibitor (Promega) was added to the beads and incubated for 2 hours at 4°C/3rpm ...
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bioRxiv - Cell Biology 2023Quote: ... Caspase-Glo® 3/7 reagent (Promega) was added to each well as per manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... For SUMO1-3 thrombin (16 U; Promega) was used for cleavage in buffer (20 mM Tris-HCl pH 8.4 ...
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bioRxiv - Zoology 2022Quote: ... and 3% 20mg/ml Proteinase K (Promega). This digestion eliminates cellular material while leaving the spongin network intact ...
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bioRxiv - Molecular Biology 2024Quote: ... Caspase-Glo 3/7 luciferase substrate (Promega) was added in a 1:1 ratio to each well followed by gentle shaking for 30 s ...
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bioRxiv - Cell Biology 2024Quote: ... 3 μL of FuGeneHD (Promega Cat# E2311) was added and incubated for an additional 15 min ...
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bioRxiv - Cancer Biology 2024Quote: The Caspase-Glo 3/7 Assay (Promega) was used to measure apoptosis in meningioma cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Caspase-Glo 3/7 3D (Promega, G8981) was used to analyze A375 spheroid apoptosis induced by different treatments.
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-β-galactosidase (Promega, Z378A; 1/100); mouse anti-Myc (Santa Cruz Biotechnology ...