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Citations for Promega :
51 - 100 of 184 citations for Urotensin II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 1μg of total RNA was retrotranscribed with Improm II Reverse Transcriptase (Promega). cDNA was mixed with iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... along with Luciferase Assay Reagent II and Stop & Glo® Reagent (Promega), following the manufacturer’s protocol.
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bioRxiv - Plant Biology 2023Quote: ... first-strand cDNA was synthesized using the ImProm-II Reverse Transcriptase (Promega). Three cDNA samples derived from three independent RNA extractions were synthesized and amplified using Luna®Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... and reverse transcribed using the Im-Prom-II Reverse Transcription System (Promega, A3800) and random hexamer primers ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription was performed using the ImProm-II Reverse Transcription System (Promega, A3800) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was yielded from purified mRNA using ImProm II Reverse Transcription kit (Promega). Quantitative-RT PCR was then performed using the SensiFAST SYBR Green Hi-ROX master mix (Bioline) ...
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bioRxiv - Microbiology 2019Quote: Reverse transcriptase (RT)-PCR was carried out using ImProm-II reverse transcriptase (Promega) following the manufacturer’s protocol with 1 µg of RNA ...
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bioRxiv - Physiology 2020Quote: ... with cDNA produced using the ImProm-II RT system (Promega, Madison, WI, USA). Real-time quantitative PCR was performed using a Prism 7000 (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... DNase digested and reverse-transcribed using the Improm-II Reverse Transcription System (Promega). qPCR was performed from 100 ng of gDNA using the Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA synthesis was performed using ImProm-II™ Reverse Transcriptional System (A3800 - Promega) with 1 ug of RNA and RT-qPCR reactions were performed using GoTaq® qPCR Master Mix (A6002 - Promega) ...
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bioRxiv - Biochemistry 2023Quote: ... in which 50 μL LAR II and 25 μL Stop & Glo reagent (Promega) were added to each well.
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bioRxiv - Genetics 2024Quote: ... cDNA was synthesized using the ImProm-II™ Reverse Transcription System (Promega, USA). The mRNA composition was established by PCR with plasmid-specific primers ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was reverse transcribed into cDNA using a ImProm-II Reverse Transcription System (Promega). cDNA was mixed with Luna Universal qPCR Master Mix (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA synthesis was performed using random hexamers and ImProm-II™ Reverse Transcriptase (Promega). qPCR was performed with Power SYBR® Green (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... the RNA was reverse-transcribed using ImProm-II™ Reverse Transcriptase (Promega, Massachusetts, USA). Specific intron-spanning primers were used in the PCR and qPCR (Supplementary Table S4) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA (1 μg) was reverse transcribed with ImProm-II Reverse Transcriptase (Promega, Madison, Wisconsin) using oligo(dT ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was prepared using ‘ImProm-II™ Reverse Transcription System’ (Promega cat No: A3800) according to the kit protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Complementary DNA (cDNA) was prepared using ImProm-II™ reverse transcription kit (Promega, A3800) and 10 ng of cDNA were used as template for each real-time PCR reaction ...
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bioRxiv - Cancer Biology 2019Quote: ... Reverse transcription was carried out using the ImProm-II™ Reverse Transcription System (Promega) using Oligo (dT)20 oligonucleotides for poly-A tail detection ...
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bioRxiv - Neuroscience 2019Quote: ... or mCherry along with (ii) pGlosensorTM -22F cAMP biosensor plasmid (Promega Corp., Madison, WI), which encodes a modified form of firefly luciferase with a fused cAMP binding moiety providing a biosensor for the direct detection of cAMP signalling in live cells ...
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bioRxiv - Pathology 2020Quote: ... 30 U of RNasin Ribonuclease Inhibitor and 1.5 µL of ImProm-II™ (Promega). The following parameters were applied ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA synthesis was performed by the ImProm-II™ Reverse Transcription System (Promega) and oligo(dT) ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA synthesis was carried out using random hexamers and Improm-II reverse transcriptase (Promega). The resulting cDNA was used for qPCR via iQ SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using random hexamers and ImProm-II™ Reverse Transcriptase (Promega). Quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2022Quote: ... and first-strand complementary DNA (cDNA) was synthesized using ImProm-II reverse transcriptase (Promega). Quantitative Reverse Transcription PCR analysis was performed using a CFX96 Real-Time System (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... followed by reverse-transcription into first-strand cDNA using ImProm II reverse transcriptase (Promega), anchored oligo(dN-T20 ...
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bioRxiv - Immunology 2023Quote: ... cDNA was synthesized by the ImProm-II™ Reverse Transcriptase system (Promega, Cat# A3801) using 100 ng RNA per reaction ...
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bioRxiv - Pathology 2023Quote: ... We reverse-transcribed cDNA from total RNA using the ImProm II Kit (Promega, US) with Random primers ...
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bioRxiv - Microbiology 2020Quote: ... RNA was reverse transcribed to cDNA using the ImProm-II™ Reverse Transcription System (Promega) and the synthesized cDNA was used for real-time analysis using the SYBR Green PCR Master Mix kit (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... and a Modulus II Microplate Multimode reader (Turner BioSystem) or a Glowmax Navigator luminometer (Promega), as described previously(24) ...
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bioRxiv - Genetics 2021Quote: ... and cDNA was synthesized by ImProm-II™ Reverse Transcriptase kit (Promega, Madison, WI, USA). qRT-PCR was performed by using StepOne Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... Reverse-transcriptase reactions were performed with the ImProm-II Reverse Transcription System (Promega, Madison, USA) using 2 μg total RNA ...
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bioRxiv - Molecular Biology 2019Quote: ... The cDNA was reverse-transcribed using the ImProm-II™ Reverse Transcription System (A3800; Promega). The expression levels of the ANRIL ...
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bioRxiv - Neuroscience 2020Quote: Total mRNA was isolated from 25 to 35 Drosophila heads following the RNeasy mini kit extraction protocol from Qiagen and reverse transcribed with oligo(dT)15 and the ImProm-II Reverse Transcription System (Promega) according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... 1.0 ug of total RNA was reverse-transcribed with ImProm-II reverse-transcriptase (Promega, USA) and random hexamers (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... and converted to cDNA using the ImProm-II Reverse Transcription System kit (Promega, Madison, USA).
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bioRxiv - Microbiology 2021Quote: ... the RNA was reverse transcribed to cDNA using the ImProm-II reverse transcription system (Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized from nuclear and cytoplasmic RNA using Improm II RT (Promega, Cat. #A3800), and cDNA levels were measured by qPCR using the Brilliant III Ultra-Fast qPCR Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... First-strand cDNA synthesis was carried out with the ImProm-II Reverse Transcription System (Promega). Quantitative real-time PCR analysis was performed in a QuantStudio 5 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2019Quote: ... Reverse-transcriptase reactions were performed with the ImProm-II Reverse Transcription System (Promega, Madison, USA) using 2 μg total RNA ...
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bioRxiv - Genomics 2021Quote: HDAC activity assays were performed using the HDAC GLO I/II assay kit (Promega, G6430) in 96 well plates following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... and (ii) HALO tag was incubated with primary Anti-HaloTag® Monoclonal Antibody (Promega #G9211) diluted 1:1 000 with secondary antibody Anti-Mouse IgG (Fc specific ...
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bioRxiv - Developmental Biology 2022Quote: ... one microgram of RNA was reverse transcribed using the ImProm-II Reverse Transcription System (Promega). Quantitative RT-PCR was performed in triplicate with custom-designed oligos using the CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... one microgram of RNA was reverse transcribed using the ImProm-II Reverse Transcription System (Promega), thereafter qRT-PCR was performed in triplicate with custom designed oligos (Table S3 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and reverse transcription was carried out using the Improm-II reverse transcriptase (Promega, Medison, USA) under the conditions described in the kit manual ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was synthesized in duplicates from 100 ng total RNA using ImProm II reverse transcriptase (Promega), RNAsin (Promega ...
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bioRxiv - Microbiology 2021Quote: ... with a Luciferase Assay Reagent II and a Stop & Glo® Reagent (Promega Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Glycosylation and cysteine mutations were made with the Quick Change II Site-Directed Mutagenesis kit (Promega). A synthetic construct coding for the hemopexin sequence without any cysteines apart from cys188 was purchased from Genescript ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA synthesis was carried out using the Improm-II reverse transcriptase according to Manufacturer’s instructions (Promega). Gene expression analysis was carried out using the Brilliant SYBR Green QPCR Reagents on a Stratagene MX3000P qPCR system according to the instructions of the manufacturer and the primers described in Table 3 ...
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bioRxiv - Cell Biology 2021Quote: ... Complementary DNA (cDNA) was synthesized following manufacturer’s instructions using the ImProm II Reverse Transcriptase system (Promega) from 500 ng of total RNAs for mycelial samples and 650 ng of total RNAs for sheath samples ...