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Citations for Promega :
51 - 100 of 1957 citations for Poly rC Binding Protein 4 PCBP4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 10μM of 2-(4-Morpholinyl)-8-phenyl-4 H-1-benzopyran-4-one (Ly294002: Promega, #V120A), 50ng/ml of Activin A (Stemcell Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were detected with horseradish peroxidase (HRP)-conjugated rabbit or mouse secondary antibody [1:1,000 dilution] (Promega). The chemiluminescence signal was imaged using an Odyssey XF Imager (LI-COR ...
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bioRxiv - Cancer Biology 2019Quote: ... Marinnisen and the pG5 Luc (minimum promoter containing 5 sites for GAL4 binding) was purchased from Promega. The expression plasmid for Nrf2 WT ...
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bioRxiv - Synthetic Biology 2019Quote: ... Poly-C tails were added to the fragmented DNA using terminal deoxynucleotidyl transferase (Promega). Then ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were resolved in reducing and non-reducing SDS-PAGE gels and membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-human p75 intracellular domain (1:1000, Promega); mouse monoclonal anti-HA (1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... The antibodies used for western blotting were: anti-HALO (1:1000 in 0.5% milk TBST, 4°C o/n, Promega G9211), anti-GAPDH (1:10000 in TBST ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated with the following primary antibodies diluted in TBS-T overnight at 4°C: mouse anti-HaloTag (1:5,000; Promega, G9211), mouse anti-Flag M2 (1:2000 ...
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bioRxiv - Genomics 2020Quote: ... 4 µL RNase-A (4 mg-mL-1, Promega, Madison WI) was incubated at 37 °C for one hour vortexing every 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Annexin V binding to PS was measured using the RealTime-Glo™ Annexin V luminescence assay (#JA1000; Promega), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... which was subsequently cloned downstream of Luc+ Poly (A) in the pGL3 promoter plasmid (Promega) and named as Ens-E ...
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bioRxiv - Molecular Biology 2023Quote: ... Sweden) was engineered by inserting the PGK-NLuc-poly(A) cassette from pNL1.1.PGK (Promega) plasmid into virus genome ...
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bioRxiv - Microbiology 2021Quote: ... Proteins separated on the second gel were transferred to a PVDF membrane and DivJ levels were measured using Western blots by probing the protein with anti-HaloTag antibody (Promega). Due to the requirement of growing cells in low phosphate media ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were transferred by semi-dry Western Blot onto PVDF membranes visualised with polyclonal antibody and BCIP/NBT (Promega, S3771).
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bioRxiv - Molecular Biology 2022Quote: ... Expression of Halo-bZIP and SBP-bZIP proteins were confirmed by western blotting using anti-HaloTag monoclonal antibody (Promega, G9211) and anti-SBPTag mouse anti-human antibody (MilliporeSigma ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of FgSte3-NLuc donor protein produced was also validated performing immunoblotting using anti-Nluc antibody (Promega cat#7000), see details below.
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bioRxiv - Cell Biology 2021Quote: ... The samples were diluted with 20 mM HEPES pH 8.0 to a urea concentration of 2 M and the proteins were digested with 4 µl Trypsin/LysC (Promega V5073: 20ug + 80uL 50mM acetic acid) for 4 hours at 37°C and boosted with an extra 2µl Trypsin/LysC (Promega V5073 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 4% PEG-8000 (Promega), 2% FBS (Biosera) ...
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bioRxiv - Genomics 2023Quote: ... 4 mM DTT (Promega), and 1x CutSmart buffer (NEB ...
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bioRxiv - Microbiology 2020Quote: ... After washing with 150 μL of binding buffer four times the samples were subjected to proteolytic digestion using 1.2 μg trypsin (sequencing grade, Promega) for 2h at 47°C ...
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bioRxiv - Cancer Biology 2022Quote: ... a 3kb region of the promoter containing HIC1 binding sites was amplified by PCR from human genomic DNA and cloned into the pGl4.26-luc vector (Promega); primers are listed in supplementary Table S1 ...
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bioRxiv - Physiology 2024Quote: The enhancer region containing the FXR binding element was amplified by PCR and cloned into the pGL4.23 vector (Promega). HepG2 cells were transiently transfected with FXR (100 ng/well ...
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bioRxiv - Microbiology 2019Quote: ... Cells were lysed 6 hours after poly I:C transfection using 100μL 1X passive lysis buffer (Promega). Plates were frozen for at least 30 minutes at −80°C before thawing and reading on a GloMax Multi plate reader (Promega) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were seeded in poly-D-lysine coated plates and transfected with FuGENE® HD (Promega) the following day according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... anti-goat alkaline phosphatase-linked antibody and alkaline phosphatase substrate (5-bromo-4-choloro-3-indolyl 1-phosphate and nitroblue tetrazolium) from Promega (Southampton, UK); a hydroxamate-based MMP inhibitor CT-1746 (N1-[2-(S)-(3,3-dimethylbutanamidyl)]-N4-hydroxy-2-(R)-[3-(4-chlorophenyl)-propyl]-succinamide ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 24-48hrs in the blocking solution containing chicken anti-human NT-4/5 antibody (10 mg/ ml, Promega, Madison, WI) or rabbit anti-human NT-4/5 (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079) to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Molecular Biology 2023Quote: Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Molecular Biology 2021Quote: The pGL-AP-1 plasmid containing 6 consensus AP-1 binding sites was co-transfected with the pRL-CMV plasmid (Promega) into cells using TransIT 2020 transfection reagent (Mirus Bio ...
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bioRxiv - Molecular Biology 2021Quote: The minimal CYC1 promoter with seven tetracycline binding sites was amplified from the yeast Tet-Promoters collection35 with YG4866/YG4867 and cloned into pGEM-Teasy (Promega) to create pCB4695 ...
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bioRxiv - Microbiology 2020Quote: ... containing the specific primers-probe binding sites were synthesized for NoV GII and cloned into the pGEM-T Vector (Promega) resulting in the NoV-GII plasmids ...
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bioRxiv - Molecular Biology 2019Quote: Validation of hsa-miR-548ba and hsa-miR-7973 direct binding to target gene 3’UTR was performed by using Dual-Glo Luciferase assay system (Promega) according to the user manual ...
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bioRxiv - Genomics 2020Quote: ... Viral RNA was extracted using the silica guanidinium isothiocyanate binding method (12) adapted for the ThermoFisher Kingfisher using paramagnetic silica particles (Magnesil, Promega).
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bioRxiv - Developmental Biology 2019Quote: ... together with a TOPFLASH luciferase reporter construct containing synthetic Tcf-binding sites (Korinek et al., 1998) and a Renilla-luciferase reporter (Promega) for normalization ...
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bioRxiv - Evolutionary Biology 2020Quote: To generate the pGl3-STK38-P luciferase reporter,1.2 kb of DNA upstream the STK38 TTS promoter region with ZNF611 binding motif was amplified by polymerase chain reaction (PCR) and cloned into the Firefly luciferase reporter plasmid pGL3-Basic (Promega) using KPN1 and XHO1 restriction sites.0.7 kb of DNA upstream the STK38 TTS promoter region without ZNF611 binding motif was cloned into pGL3-Basic using same restriction sites to generate the pGl3-STK38-ΔP luciferase reporter ...
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bioRxiv - Microbiology 2022Quote: ... containing the specific primers-probe binding sites were synthesized for NoV GII and cloned into the pGEM-T Vector (Promega), resulting in the NoV-GII plasmids ...
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bioRxiv - Developmental Biology 2024Quote: ... a minimal promoter (5′-AGACACTAGAGGGTATATAATGGAAGCTCGACTTCCAG-3′) and 8x Gli1-binding sites (110) were cloned into the pGL3-Basic vector (Promega), in which Firefly luciferase was replaced with NanoLuc luciferase amplified from the pNLF-N [CMV/Hygro] vector (Promega ...
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bioRxiv - Biochemistry 2024Quote: The 3′UTR fragment of the human PHF8 gene having native or mutated miR-22-3p or miR-1229-3p binding sites (Supplementary Figure S1) was cloned into the pmirGLO vector (Promega) cut with XbaI and DraI restriction enzymes (New England BioLabs) ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then digested with LysC at 1ug/100ug protein (Promega) for 3 h at room temperature (RT ...
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bioRxiv - Plant Biology 2020Quote: ... Fusion proteins were purified using MagnetHis™ protein purification system (Promega) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... Proteins were precipitated through the addition of Protein Precipitation Solution (Promega) followed by vigorous vortexing ...
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bioRxiv - Plant Biology 2024Quote: ... plasmids containing the corresponding open reading frames in a modified pSP64-poly(A) vector (Promega, Madison, WI) with the respective additional restriction site downstream of the poly(A ...
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bioRxiv - Cell Biology 2019Quote: ... 4 μg (Promega, Cat. # v5113) was added to digest proteins to generate peptides overnight at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: ... β-mercaptoethanol (3.4 × 10−4%, Promega) and Penicillin (1% ...
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bioRxiv - Bioengineering 2020Quote: ... β-mercaptoethanol (3.4 × 10−4%, Promega) and Penicillin (1% ...
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bioRxiv - Bioengineering 2020Quote: ... β-mercaptoethanol (3.4 × 10−4%, Promega) and Penicillin (1% ...
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bioRxiv - Microbiology 2021Quote: ... 4 μl 5× Buffer (Promega), 2 μl MgCl2 (25mM) ...
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bioRxiv - Microbiology 2023Quote: ... 4 mM magnesium chloride (Promega), 0.4 mM dNTPs (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... containing 4% D-luciferin (Promega). The luciferase substrate permeated the cells for 120 min at 37 ℃ ...
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bioRxiv - Cell Biology 2024Quote: ... 4% PEG 8000 (Promega, V3011), 5 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... Mass Spec-Compatible Human Protein Extracts, Cat# V6951) and digested yeast protein extract (Mass Spec-Compatible Yeast Protein Extracts, Cat# V7461) were from Promega, commercial human plasma samples (Human Source Plasma ...