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Citations for Promega :
51 - 100 of 149 citations for Neuromedin S Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: The fused peptide–mRNA was subsequently reverse transcribed using MMLV RT Rnase H-(Promega) for 1 h at 42 °C ...
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bioRxiv - Microbiology 2020Quote: ... Luciferase intensity was measured with a GloMax luminometer (10-s signal integration; Promega BioSystems).
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bioRxiv - Biophysics 2019Quote: ... Generation of tryptic peptides for mass spectrometry was performed with the aid of ProteaseMax (Promega) detergent ...
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bioRxiv - Biochemistry 2021Quote: ... chymotrypsin and peptide-N-glycosidase F (PNGase F) were obtained from Promega (Madison, WI, USA). Centrifugal filters (0.5 mL ...
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bioRxiv - Biochemistry 2023Quote: ... The methyltransferase reaction product S-adenosylhomocysteine was quantified using MTase-Glo kit (Promega, Wisconsin, USA) following the manufacturer’s protocol (43) ...
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bioRxiv - Cell Biology 2023Quote: ... and applied to S-Traps (micro; Protifi) for tryptic digestion (sequencing grade; Promega, Madison, WI) in 50 mM TEAB ...
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bioRxiv - Bioengineering 2024Quote: ... The Glucose-Glo and Lactate-Glo kits (Cat #’s J6021 and J5021, Promega, Madison, WI) were used to measure the apparent glucose and lactate concentrations in media samples according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was probed with α-Halotag mouse monoclonal antibody (Promega, diluted 1:1000 in Blocking Buffer), washed 3 times in TBS-Tween ...
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bioRxiv - Molecular Biology 2020Quote: ... then probed with goat α-mouse HRP-conjugated secondary antibody (Promega, diluted 1:10000 in Blocking Buffer) and finally washed 3 times in TBS-Tween ...
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bioRxiv - Biochemistry 2022Quote: ... the primary antibody was diluted in blocking buffer as follows: Anti-HaloTag (1:1000; Promega Corporation; G9281); anti-PHC1 antibody (1:500 ...
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bioRxiv - Biochemistry 2022Quote: ... and then peptides were collected after enzymatic digestion using trypsin (Trypsin Gold, Promega, Fitchburg, WI, USA); the peptides were desalted by Oasis HLB cartridges (Waters ...
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bioRxiv - Immunology 2022Quote: ... Proteins were digested into peptides with 12μL of 1:10 Trypsin Gold (Promega, Madison, Wisconsin, V528A) in 50mM TEAB per sample ...
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bioRxiv - Biophysics 2023Quote: ... the proteins were digested into tryptic peptides by incubation with 1□µg sequencing grade trypsin (Promega) overnight at 37□°C ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Sigma-Aldrich)–coated dishes or glass coverslips with 30 ng/ml of 2.5 S mouse NGF (G5141, Promega) in the Neurobasal medium containing B27 supplement (17504044 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... S-Traps were loaded with 125 μL trypsin solution (50 ng/μL in 50mM TEAB; Promega) and incubated at 47°C for 2 hours ...
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bioRxiv - Microbiology 2021Quote: Peptides from cell-surface/biofilm-ECM-associated proteins were released via a Trypsin/Lys-C mix (Promega) at a final concentration of 50 ng µL-1 ...
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bioRxiv - Molecular Biology 2019Quote: ... Instrument quality control was monitored using the Promega 6×5 LC-MS/MS Peptide Reference Mix (Promega) before and after each MS experiment run ...
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bioRxiv - Neuroscience 2023Quote: A pET25 vector encoding the cell penetrating peptide SynB1 underwent restriction digest using BamHI (Promega, catalog #R6021) for 1 hour at 37 °C and SfiI (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... At 1 × s after injection of 20 µl of the substrate solution (Renilla Luciferase Assay System, Promega), relative light units (RLUs ...
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bioRxiv - Cell Biology 2020Quote: KNL-2-MBP fragments were [35]S labeled using TnT® Quick Coupled Transcription/Translation System (Promega). 10 μl of the in vitro translation lysate was incubated for 1hr at 4°C with 50 μg CENP-AHCP-3(1-109)-MBP-His (in 20 mM Tris [pH7.5] ...
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bioRxiv - Immunology 2023Quote: ... VP1-3 proteins were then bound to the S-Trap column and digested with trypsin (Promega Corporation) for 2 hrs at 47°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The Nluc and BRET signals were measured (integration time: 1 s) after auto-injecting either Fz (Promega) or FFz (Promega ...
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bioRxiv - Immunology 2024Quote: ... FC tags on the S and RBD proteins were removed through treatment with Factor Xa Proteinase (Promega).
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bioRxiv - Genetics 2024Quote: ... and incubated with primary antibodies in a blocking buffer (1ξ PBS, 0.5% BSA) and then with alkaline phosphatase-conjugated secondary antibody (Promega). The color reaction was developed with NBT/BCIP solution (Nacalai Tesque).
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bioRxiv - Cancer Biology 2019Quote: ... ATP solution (5 μL of a 100 μM solution containing 0.1 μg 4:1 glycine:tyrosine peptide substrate (Promega)) was added and incubated at 37 °C for 20 min before the addition of ADP-Glo reagent (5μL ...
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bioRxiv - Biochemistry 2021Quote: ... Aliquots of reagent were used for sample labeling of a serial dilution of K562 peptide digest standard (Promega) using all heavy N labeled channels that day following manufacturer instructions ...
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bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
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bioRxiv - Biochemistry 2022Quote: ... Rat Gβ1 with an N-terminal MHHHHHHSSGLVPRGSHMASHHHHHHHHHH-tag (His16) was fused with the SmBiT subunit (peptide 86, Promega)20 via a 15 amino acid (GSSGGGGSGGGGSSG ...
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bioRxiv - Plant Biology 2023Quote: ... Total proteome peptide pools were generated by sample digestion overnight with 1:100 sequencing grade trypsin (V5113; Promega). Generated peptide pools were quantified by Nanodrop ...
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bioRxiv - Cell Biology 2023Quote: ... Peptide digestion was performed by adding 1µg of trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega Corporation, WI, USA) and incubating each sample overnight at 30°C ...
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bioRxiv - Cell Biology 2022Quote: ... The beads were collected by centrifugation at 8000 × g for 30 s or using a magnet separator (Promega), and washed three times with lysis buffer ...
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bioRxiv - Immunology 2022Quote: The ADCC activity of anti-SARS-CoV2 S IgG antibodies was determined using the ADCC Reporter Bioassay (Promega) as previously described (Dufloo et al. ...
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bioRxiv - Microbiology 2020Quote: ... Donor cells were co-transfected with 1.5 μg pCAGGS-S and 0,5 μg pmCherry-N1 using 6 μl of Fugene 6 following the manufacturer’s instructions (Promega). Acceptor cells were treated with CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... purified protein samples were prepared by S-alkylation with iodoacetamide and digestion with either with LysC/GluC (Promega) [RBD] or ...
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bioRxiv - Cell Biology 2022Quote: ... For phospho-peptide samples the volume was increased to 2,000 μl (7-fold dilution) and 30 μg Trypsin Gold (Promega) was added to each sample ...
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bioRxiv - Neuroscience 2022Quote: ... The mixture was then incubated with 1 μg of AKT substrate II peptide (CKRPRAASFAE) as the Pak4 substrate (Promega) or 1 μg of GST-Raf13 as the Pak1 substrate with a total volume of 5 μL in a 384-well plate (PerkinElmer ...
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bioRxiv - Immunology 2019Quote: ... then washed three times in lysis buffer and two times in ammonium biocarbonate (50mM) before peptide digestion (V5280, Promega). Peptides were loaded onto a 3-cm self-packed C18 capillary pre-column (Reprosil 5 μM ...
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bioRxiv - Cancer Biology 2021Quote: ... EV protein samples were alkylated the next day with 50 mM iodoacetamide for 2 hours at ambient temperature in the dark and then digested into peptides with 1.2 µg proteomics-grade trypsin/LysC (Promega) according to the SP3 protocol described by Hughes et al (2019) ...
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bioRxiv - Physiology 2022Quote: ... The proteins were cleaved into tryptic peptides by addition of 25 μl of a 0.2 μg/μl trypsin (Promega) in 50 mM ammonium bicarbonate and incubation (18 h ...
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bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
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bioRxiv - Molecular Biology 2023Quote: ... Quality control of the instrument setup was monitored using the Promega 6x5 LC-MS/MS Peptide Reference Mix (Promega) and analyzed with the PReMiS software (version 1.0.5.1 ...
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bioRxiv - Bioengineering 2023Quote: ... The reaction was initiated by the addition of 1-250 nM acylated peptide (Vivitide) and a 1:10,000 dilution of LgBiT subunit of the NanoBiT luciferase (Promega) and read using an Envision plate reader (XCite 2105 ...
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bioRxiv - Biochemistry 2023Quote: ... Rat Gβ1 was fused with a His-tag at the N terminus and with a SmBiT subunit (peptide 86, Promega)34 after a 15-amino acid linker at its C terminus ...
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bioRxiv - Biochemistry 2023Quote: Serine/threonine phosphatase activity assay of PP2A was performed using the artificial p-peptides RRA(pT)VA substrate (Promega) in lysis buffer containing 1 mM MnCl2 at 70 °C 26 ...
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bioRxiv - Systems Biology 2021Quote: ... alkylated in the dark with iodoacetamide and applied to S-Traps (micro; Protifi) for tryptic digestion (sequencing grade; Promega) in 50 mM TEAB ...
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bioRxiv - Cancer Biology 2020Quote: ... alkylated in the dark with iodoacetamide and applied to S-Traps (mini; Protifi) for tryptic digestion (sequencing grade; Promega) in 50 mM TEAB ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) (HyClone) with 10% fetal bovine serum FBS (HyClone) and 1% penicillin-streptomycin (P.S.) (Promega) at 37 °C in a 5% CO2 atmosphere.
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bioRxiv - Microbiology 2022Quote: ... either with SARS-CoV-2 S and Jun or with ACE2 and Fos plus the pRL Renilla Luciferase (Promega) to normalize the signal ...
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bioRxiv - Microbiology 2023Quote: ... Sequences of all plasmids were verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems, Cat# 063001) with a Spectrum Compact CE System (Promega). The psPAX2-IN/HiBiT plasmid was a kind gift from Dr ...
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bioRxiv - Biochemistry 2023Quote: ... Enzymatic digestion and detergent removal were performed using the S-Trap device and Trypsin Gold (Promega, Madison, Wisconsin, USA) following the manufactures recommended protocol ...