Labshake search
Citations for Promega :
51 - 100 of 413 citations for NKX2 4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... Proteins were digested into peptides with 12μL of 1:10 Trypsin Gold (Promega, Madison, Wisconsin, V528A) in 50mM TEAB per sample ...
-
bioRxiv - Biophysics 2023Quote: ... the proteins were digested into tryptic peptides by incubation with 1□µg sequencing grade trypsin (Promega) overnight at 37□°C ...
-
bioRxiv - Cell Biology 2019Quote: ... 4 μg (Promega, Cat. # v5113) was added to digest proteins to generate peptides overnight at 37 °C ...
-
bioRxiv - Bioengineering 2020Quote: ... β-mercaptoethanol (3.4 × 10−4%, Promega) and Penicillin (1% ...
-
bioRxiv - Bioengineering 2020Quote: ... β-mercaptoethanol (3.4 × 10−4%, Promega) and Penicillin (1% ...
-
bioRxiv - Bioengineering 2020Quote: ... β-mercaptoethanol (3.4 × 10−4%, Promega) and Penicillin (1% ...
-
bioRxiv - Microbiology 2021Quote: ... 4 μl 5× Buffer (Promega), 2 μl MgCl2 (25mM) ...
-
bioRxiv - Microbiology 2023Quote: ... 4 mM magnesium chloride (Promega), 0.4 mM dNTPs (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: Peptides from cell-surface/biofilm-ECM-associated proteins were released via a Trypsin/Lys-C mix (Promega) at a final concentration of 50 ng µL-1 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Instrument quality control was monitored using the Promega 6×5 LC-MS/MS Peptide Reference Mix (Promega) before and after each MS experiment run ...
-
bioRxiv - Neuroscience 2023Quote: A pET25 vector encoding the cell penetrating peptide SynB1 underwent restriction digest using BamHI (Promega, catalog #R6021) for 1 hour at 37 °C and SfiI (New England Biolabs ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4 μg of RNase A (Promega), and 40 units of RNase ONE (Promega ...
-
bioRxiv - Cell Biology 2023Quote: ... 4 µl RT buffer 5x (Promega), 2.5 μl dNTPs 2.5 mM ...
-
bioRxiv - Genetics 2024Quote: ... and incubated with primary antibodies in a blocking buffer (1ξ PBS, 0.5% BSA) and then with alkaline phosphatase-conjugated secondary antibody (Promega). The color reaction was developed with NBT/BCIP solution (Nacalai Tesque).
-
bioRxiv - Biochemistry 2021Quote: ... Aliquots of reagent were used for sample labeling of a serial dilution of K562 peptide digest standard (Promega) using all heavy N labeled channels that day following manufacturer instructions ...
-
bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
-
bioRxiv - Biochemistry 2022Quote: ... Rat Gβ1 with an N-terminal MHHHHHHSSGLVPRGSHMASHHHHHHHHHH-tag (His16) was fused with the SmBiT subunit (peptide 86, Promega)20 via a 15 amino acid (GSSGGGGSGGGGSSG ...
-
bioRxiv - Plant Biology 2023Quote: ... Total proteome peptide pools were generated by sample digestion overnight with 1:100 sequencing grade trypsin (V5113; Promega). Generated peptide pools were quantified by Nanodrop ...
-
bioRxiv - Cell Biology 2023Quote: ... Peptide digestion was performed by adding 1µg of trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega Corporation, WI, USA) and incubating each sample overnight at 30°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... 4 ng of Renilla (pRL-CMV, Promega), and 0.12 μL of X-tremeGENE HP ...
-
bioRxiv - Genetics 2022Quote: ... 4 units RQ1 RNase-free DNase (Promega), protease and phosphatase inhibitor mix (Halt ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4 U RNaseIn plus Ribonuclease Inhibitor (Promega), 1 mM NaF and 100 µM luciferin (Promega)] to a final volume of 15 µl with water ...
-
bioRxiv - Cell Biology 2019Quote: ... containing 4 μg/ml RNase A (Promega) for 10 min at 37°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... 4 µl of RQ DNAse I (Promega), 21 µl of nuclease-free water and 5 µl of CaCl2 (10mM ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 μL/well of Bright-Glo (Promega) was dispensed ...
-
bioRxiv - Genomics 2020Quote: ... 4 × Protease Inhibitor (Promega, Cat. No. G6521)] ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2 or 4 (Promega Madison, WI, USA) (62) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 4 U/μl reverse transcriptase (Promega). 2 µl of cDNA were used for Real-Time PCR with self-designed primers and SYBR green reaction (Roche) ...
-
bioRxiv - Developmental Biology 2023Quote: ... 4 µl 5x reaction buffer (Promega, M289A), 2.4 µl MgCl2 (Promega ...
-
bioRxiv - Developmental Biology 2023Quote: ... 4 µl 5x reaction buffer (Promega, M289A), 2.4 µl MgCl2 (Promega ...
-
bioRxiv - Molecular Biology 2023Quote: ... 4 ng of Renilla control plasmid (Promega) and 0.62 μL of Lipofectamine 2000 Transfection Reagent (InvitrogenTM) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 4 μl of FuGENE 6 (Promega) in 100 μl of OPTI-DMEM ...
-
bioRxiv - Cell Biology 2022Quote: ... For phospho-peptide samples the volume was increased to 2,000 μl (7-fold dilution) and 30 μg Trypsin Gold (Promega) was added to each sample ...
-
bioRxiv - Neuroscience 2022Quote: ... The mixture was then incubated with 1 μg of AKT substrate II peptide (CKRPRAASFAE) as the Pak4 substrate (Promega) or 1 μg of GST-Raf13 as the Pak1 substrate with a total volume of 5 μL in a 384-well plate (PerkinElmer ...
-
bioRxiv - Immunology 2019Quote: ... then washed three times in lysis buffer and two times in ammonium biocarbonate (50mM) before peptide digestion (V5280, Promega). Peptides were loaded onto a 3-cm self-packed C18 capillary pre-column (Reprosil 5 μM ...
-
bioRxiv - Cancer Biology 2021Quote: ... EV protein samples were alkylated the next day with 50 mM iodoacetamide for 2 hours at ambient temperature in the dark and then digested into peptides with 1.2 µg proteomics-grade trypsin/LysC (Promega) according to the SP3 protocol described by Hughes et al (2019) ...
-
bioRxiv - Physiology 2022Quote: ... The proteins were cleaved into tryptic peptides by addition of 25 μl of a 0.2 μg/μl trypsin (Promega) in 50 mM ammonium bicarbonate and incubation (18 h ...
-
bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
-
bioRxiv - Molecular Biology 2023Quote: ... Quality control of the instrument setup was monitored using the Promega 6x5 LC-MS/MS Peptide Reference Mix (Promega) and analyzed with the PReMiS software (version 1.0.5.1 ...
-
bioRxiv - Bioengineering 2023Quote: ... The reaction was initiated by the addition of 1-250 nM acylated peptide (Vivitide) and a 1:10,000 dilution of LgBiT subunit of the NanoBiT luciferase (Promega) and read using an Envision plate reader (XCite 2105 ...
-
bioRxiv - Biochemistry 2023Quote: ... Rat Gβ1 was fused with a His-tag at the N terminus and with a SmBiT subunit (peptide 86, Promega)34 after a 15-amino acid linker at its C terminus ...
-
bioRxiv - Biochemistry 2023Quote: Serine/threonine phosphatase activity assay of PP2A was performed using the artificial p-peptides RRA(pT)VA substrate (Promega) in lysis buffer containing 1 mM MnCl2 at 70 °C 26 ...
-
bioRxiv - Systems Biology 2021Quote: ... Collisional energy was ramped stepwise as a function of ion mobility.24 Peptide loading was normalized to the total ion chromatograms from 200 ng injections of K562 whole cell lysate standard (Promega).
-
bioRxiv - Biophysics 2020Quote: ... Proteins were digested to peptides by addition of 25 μl of 50 mM ammonium bicarbonate containing 5 μg of trypsin (Promega) and incubating (37°C ...
-
bioRxiv - Immunology 2020Quote: The strategy for genome-engineering to tag HLA-B with the HiBit peptide was developed based on a previous publication from Promega Corporation ...
-
bioRxiv - Biochemistry 2019Quote: A commercial K562 cell digest supplemented with the 6 x 5 LC-MS/MS Peptide Reference Mix (QC4Life Reference Standard, Promega) was used as quality control (QC ...
-
bioRxiv - Bioengineering 2019Quote: ... after removal of its original signal peptide and was further fused to Halo-tag derived from HaloTag Control Vector (Promega) at the C-terminus ...
-
bioRxiv - Synthetic Biology 2019Quote: ... The supernatant was taken to measure the amount of secreted HiBiT peptide using the Nano-Glo HiBiT Extracellular Detection System (Promega).
-
bioRxiv - Synthetic Biology 2019Quote: ... and the supernatant was taken to measure the amount of secreted HiBiT peptide using the Nano-Glo HiBiT Extracellular Detection System (Promega). The resulting luminescence signals were normalized based on the cell viability that was measured using the PrestoBlue Cell Viability Reagent (Thermo).
-
bioRxiv - Cell Biology 2022Quote: Methylation of peptides (PSL, Heidelberg, Germany, Table S5) was performed using the bioluminescence-based MTase-GloTM Assay (Promega, Cat#V7601) according to the manufacturer’s protocol ...