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Citations for Promega :
51 - 100 of 209 citations for LS Tetrasaccharide a LSTa Sialyl lacto N tetraose a >90% NMR Sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: Human PAR2 with N-terminal nano-luciferase (nLuc, Promega) and C-terminal enhanced Yellow Fluorescent Protein (eYFP ...
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bioRxiv - Genomics 2020Quote: ... 0.1% RNAsin plus RNase inhibitor (Promega, Cat. N: N261A) in PBS (wash buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... and Asp-N were purchased from Promega (Madison, WI), and a-lytic protease was obtained from New England BioLabs (Ipswich ...
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bioRxiv - Biochemistry 2024Quote: ... for making N-terminal fusions to HaloTag7 (HT7, Promega). Fusions of the following proteins were expressed in bacteria ...
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bioRxiv - Genomics 2024Quote: ... n=2) using the multiplexed ApoTox-Glo assay (Promega). Using 96-well plates ...
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bioRxiv - Genomics 2020Quote: ... 0.5% Sodium Deoxycholate) supplemented with 1X Protease Inhibitor Cocktail (Promega), 200 U of Murine RNase Inhibitor (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% Sodium Deoxycholate) supplemented with 1X Protease Inhibitor Cocktail (Promega), 200 U of Ribolock (ThermoFisher) ...
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bioRxiv - Bioengineering 2023Quote: ... mixed with 0.1 volume of 3M Sodium Acetate (Promega, UK) and 1 volume of isopropanol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended in Hank’s buffered salt solution (HBSS) containing NanoGlo Live Cell Reagent (1:20; Promega) with furimazine ...
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bioRxiv - Cell Biology 2023Quote: ... A 90 μl volume of each culture and 10 μl of the furimazine (1 in 200 dilution; Promega) were incubated in a Cellstar non-transparent white 96-well microplate for one h and the bioluminescence intensity was monitored with a Tecan Infinite 200 PRO plate reader every 30 s ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from 125 µL of nasal or lung wash using ISOGEN-LS (Nippon gene, Toyko, Japan) and purified using a Maxwell RSC 48 Instrument (Promega, Madison, WI) with a Maxwell RSC miRNA Plasma and Serum Kit (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... 20 µL of the MTS salt containing CellTiter 96 Aqueous One Solution reagent (Promega, Madison, WI) was added and incubated for further 1-2 h at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-βIII tubulin was obtained from Promega (Cat. N° G712A) (Madison ...
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bioRxiv - Biochemistry 2020Quote: ... Asp-N (gold mass spectrometry grade) was purchased from Promega.
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bioRxiv - Biochemistry 2022Quote: ... Chymotrypsin (#V1062) and Asp-N (#V1621) were purchased from Promega. Bryostatin-1 (#2383 ...
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Pushed to the edge: hundreds of myosin 10s pack into filopodia and could cause traffic jams on actinbioRxiv - Cell Biology 2024Quote: ... The construct includes an N-terminal Halotag (Promega; GenBank: JF920304.1), the full-length human Myo10 sequence (nucleotide sequence from GenBank ...
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bioRxiv - Physiology 2024Quote: ... except that the qPCR kit from Promega (catalog N° A6102) was used ...
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bioRxiv - Genomics 2024Quote: ... 1:100 RNAsin plus RNase inhibitor (Promega, Cat. N.: N261A), PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Chymotrypsin (#v1062) and Asp-N (#v1621) were purchased from Promega. GluC (#90054 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% (w/v) sodium dodecyl sulfate) with Protease Inhibitor Cocktail (Promega)77 ...
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bioRxiv - Molecular Biology 2021Quote: ... All the clones were transfected into the HEK-293T cells at 90% confluency using FuGene 6 as transfection reagent according to the manufacturer’s instructions (Promega). dsiRNAs targeting Rpp30 (10 nM ...
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bioRxiv - Biophysics 2022Quote: ... DTT was added to a final concentration of 3 mM and the sample was then diluted with 90 μl of 50 mM Tris before adding 0.1 μg of sequencing-grade trypsin (Promega) and incubating at 37 °C overnight ...
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bioRxiv - Bioengineering 2023Quote: ... Lysates (20 mg per well) were assessed in 96-well plates using 90 μl reconstituted Luciferase Assay Substrate (Promega) according to the manufacturer’s directions ...
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bioRxiv - Biochemistry 2024Quote: ... The translation mixtures were incubated at 30°C for 90 mins followed by the dual-luciferase reporter assay (Promega). The 2μl translation products were added into a white 384-well microplate (PerkinElmer) ...
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bioRxiv - Systems Biology 2024Quote: ... followed by 80μL Hank’s balanced salt solution (HBSS) containing 10mM HEPES and 10μM furimazine/NanoGlo Luciferase Assay Substrate (Promega). BRET measurements were performed at 37 °C using a PHERAstar Microplate Reader (BMG Labtech ...
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bioRxiv - Cancer Biology 2024Quote: ... mice were anesthetized with isoflurane and injected intraperitoneally with D-luciferin potassium salt (Promega, Madison, WI, USA). Ten to fifteen min after injection ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5% Sodium deoxycholate and 0.1% SDS) containing protease inhibitors (Promega, Madison, USA) and centrifuged at 10,000× g (30 min at 4°C) ...
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bioRxiv - Microbiology 2021Quote: N-terminal HaloTag KIF4 expressing plasmid (pFN21ASDB3041) was purchased from Promega. The N-terminal Myc-tagged KIF4 (both wild type and ATPase-null motor inactive mutant ...
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bioRxiv - Biochemistry 2024Quote: ... and 10 units of Peptide N-glycosidase F (PNGase F, Promega) enzymes according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: SETDB1 (3TD, 197-403aa) was cloned into pBiT3.1-N (Promega, #N2361) and pBiT3.1-C (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were produced from pcDNA3-based plasmids at 30 °C for 90 min using the in vitro transcription & translation (IVTT) kit (Promega), following manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: Pooled DNA extractions (minimum 90 adult mosquitoes or 3500 L1 larvae) were performed using the Wizard Genomic DNA Purification kit (Promega). A 349 bp locus containing the predicted on-target cleavage site was amplified with primers containing Illumina Nextera Transposase adapters (underlined) ...
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bioRxiv - Molecular Biology 2020Quote: The non-TOP 5′ UTR sequence of ATP5O (5′-CGGGAGAAG-3′) was identified in published nanoCAGE data 90 and inserted between the T7 promoter and Renilla luciferase in psiCHECK2 (Promega) by Gibson Assembly.
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bioRxiv - Molecular Biology 2021Quote: ... Then the plasmid constructs (150 ng) were transfected into HEK 293T cells at their 90% confluency using Dual-Luciferase Reporter Assay System (Promega). The Luciferase activity was measured 72 h (48h for DsiRNA and 24 h for plasmid constructs ...
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bioRxiv - Cell Biology 2021Quote: ... cells were again washed with DPBS and incubated with 90 μL of a 1/1000 dilution of furimazine stock solution (Promega) in 0.1 % BSA/HBSS ...
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bioRxiv - Microbiology 2023Quote: ... The columns were then washed three times with a wash solution (90% methanol; 100mM (final concentration) TEAB) before digestion with 100ug trypsin (Promega) (1/10 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were visualized on 1% agarose gels (90 V, 30-45 minutes) against a 1 kb DNA ladder (Promega). Sanger sequencing was performed by the sequencing unit at the Max Planck Institute for Evolutionary Biology ...
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bioRxiv - Cell Biology 2024Quote: ... Tris 50mM pH8.0 and samples were heated 5 min at 90°C before digestion in a mix of 500 ng of LysC (Promega) at 37°C for 2h ...
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A resource for extracellular vesicles from activated CD4 T cells that relay pro-inflammatory signalsbioRxiv - Immunology 2024Quote: ... Samples were precipitated using 1:8 vol:vol dilution of each sample in 90% methanol in 100 mM Triethylamonium bicarbonate and digested with trypsin (Promega, #V1115) overnight at 37° C ...
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bioRxiv - Immunology 2024Quote: ... samples were precipitated using 1:8 vol:vol dilution of each sample in 90% methanol in 100 mM Triethylamonium bicarbonate and digested with trypsin (Promega, #V1115) overnight at 37° C ...
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bioRxiv - Bioengineering 2024Quote: ... 1 µg of total RNA was reverse-transcribed at 42 °C for 90 min and 72 °C for 15 min using the moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega). Each cDNA sample was diluted 8-folds and used for qRT-PCR with the specific primer pairs of each gene listed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... inner salt] assay using CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS) kit (Promega, Cat# G3582) at 72 h after BKIDCs were added for all cell lines ...
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bioRxiv - Genomics 2022Quote: ... was resuspended in 1.5 mL salt-tris solution buffer supplemented with 0.2 U/ul RNasin Plus RNase Inhibitor (Promega). The tissue material was spin down at 500 x g for 5 min at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Transcription of tRNApheV and tRNAleuZ was performed with 20 U T7 RNA Polymerase (37 °C for 90 min) using the Riboprobe® in vitro Transcription System (Promega) essentially as described in the instructions manual ...
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bioRxiv - Microbiology 2020Quote: Transfection of BKPyV genomic DNA or plasmids containing the agnoprotein into RPTECs or UTA6 cells was performed at 90-95% confluency using ViaFect™ Transfection Reagent (E4982, Promega) at a reagent ...
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bioRxiv - Molecular Biology 2021Quote: ... and cells with approximately 70-90% confluency in each plate were transfected with required plasmid(s) for each assay using Fugene 6 transfection reagent (Promega, USA) or 1 mg/ml PEI (polyethyleneimine).
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bioRxiv - Microbiology 2023Quote: ABIL emulsion oil was prepared by supplementing mineral oil with 4% ABIL EM 90 (Redox, Australia) and 0.05% Triton X-100 (Promega, United States). 425 μL of ABIL oil was added to the PCR mix ...
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bioRxiv - Microbiology 2022Quote: ... Plates were further incubated for 90 minutes and fluorescence was measured (λem = 590 nm, λex = 520 nm) using a spectrophotometer (Promega Discover).
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bioRxiv - Genetics 2022Quote: ... the collected lysates (5% of which were collected as input control) were incubated with 100 μM TASQs (or 100 μM biotin as control) and 90 μg of MagneSphere® beads (Promega) for 2 h at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... We mixed the solution containing the plasmids and the nicking enzyme was incubated at 37°C for 90 min and immediately purified over the PCR extraction membrane (Wizard® SV, Promega) and specifically skipped the recommended 80°C inactivation step ...