Labshake search
Citations for Promega :
51 - 100 of 2671 citations for L Isoleucine N Fmoc 1 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... Chymotrypsin (#V1062) and Asp-N (#V1621) were purchased from Promega. Bryostatin-1 (#2383 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The urea concentration was then diluted with 17 μl of 50 mM HEPES pH 8.5 and after adding 1 μl of 0.2 mg/ml trypsin (Promega) in 0.2 mM HCl the digestion continued overnight at 37°C with interval mixing at 2000 RPM for 30 s every 15 minutes ...
-
bioRxiv - Cancer Biology 2022Quote: ... 20,000 to 50,000 cells were lysed in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 [Illumina], 10.25 μl nuclease-free water, and 0.25 μl 1% digitonin [Promega]) for 30 min at 37 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... The samples were diluted with 17 µL of 50 mM HEPES (pH 8.5) and digested with 1 µL of 0.2 mg/mL trypsin (Promega) in 0.2 mM HCl at 37°C with interval mixing at 2000 RPM for 30 sec every 15 min.
-
bioRxiv - Biochemistry 2021Quote: ... which contains a pRSET backbone with an N-terminal 6xHis tag and the insertion of a biotin tag from the PinPoint™ Xa-1 Vector (Promega, USA) in between the His tag and the first fluorescent protein ...
-
bioRxiv - Neuroscience 2022Quote: ... 10µl RNasin RNase-inhibitor (40U/l, Promega) were added ...
-
bioRxiv - Immunology 2023Quote: ... HRP-anti-Mouse IgG (H+L) (Promega).
-
bioRxiv - Microbiology 2019Quote: ... Proteins were then digested using modified trypsin (Promega, P/N V5113) at a 1:20 (w/w ...
-
bioRxiv - Microbiology 2021Quote: N-terminal HaloTag KIF4 expressing plasmid (pFN21ASDB3041) was purchased from Promega. The N-terminal Myc-tagged KIF4 (both wild type and ATPase-null motor inactive mutant ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... CMV-LgBit was made by amplifying LgBit from pBiT1.1-N (Promega) and ligating into pcDNA3.1 (+ ...
-
bioRxiv - Biochemistry 2024Quote: ... and 10 units of Peptide N-glycosidase F (PNGase F, Promega) enzymes according to manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2020Quote: ... 10 mg/L BSA and 1 mM PMSF) and assayed using the Dual-Luciferase® Reporter Assay System (Promega). Experiments were normalized to negative control set at 1.
-
bioRxiv - Genomics 2021Quote: The supernatant was magnetically removed and the beads were resuspended in 20 µl of L3 DNA linker ligation mixture (8 µl water, 5 µl 4X ligation buffer, 1 µl RNA ligase [New England Biolabs], 0.5 µl RNasin [N2615, Promega GmbH] ...
-
bioRxiv - Cell Biology 2021Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500 µl nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
-
bioRxiv - Cell Biology 2022Quote: ... were seeded onto 6-well plates coated with 0.1 mg ml−1 poly-L-lysine and transfected using FuGENE HD transfection reagent (Promega) with a mixture of third-generation lentiviral backbone vector pJHUMCS that encodes BDNF-pHluorin ...
-
bioRxiv - Neuroscience 2023Quote: ... Then the beads were incubated with 80 μL of 2 M urea in 50 mM Tris-HCL containing 1 mM DTT and 0.4 μg trypsin (Promega) at 25 °C for 1 hour while shaking at 1,000 rpm ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5 µl of the reaction was treated with 1 µl of RNase mixture (10% RNase A, Sigma + 20% RNase One, Promega), incubated at 37°C for 10 min and then mixed with 6 µl of 2 × SDS-PAGE buffer ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1 μl of 10 mM ddTTP for the sequencing reaction and 1 μl of MMLV Reverse Transcriptase RNAse H minus (Promega) were finally added and the reverse transcription was performed at 35°C for 2 minutes ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNA pellets were resuspended in 8µl of DEPC water and incubated with µl (1U DNAse) and 1 µl of 10X DNAse buffer (Ref. M6101, Promega) for 30 min at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to a concentration of urea of 2 mol L-1 and digested overnight with the addition of 2 µl sequencing-grade trypsin (Promega). After digestion ...
-
bioRxiv - Microbiology 2019Quote: ... containing 500 μl of nuclease free water (Cat. N°. P119C/Promega/U.S.A). The upper part of the swabs was cut using sterile scissors to allow closing of the tube ...
-
bioRxiv - Cancer Biology 2022Quote: ... and N-glycans were released following the addition of PNGase F (Promega) in PBS ...
-
bioRxiv - Immunology 2021Quote: ... 3) goat anti-human H+L (Promega, W403B) at 1:3,000 dilution ...
-
bioRxiv - Microbiology 2021Quote: ... Anti-rabbit IgG (H+L) HRP Conjugate (Promega) was used as the secondary antibody ...
-
bioRxiv - Cell Biology 2021Quote: ... and Anti-Rabbit IgG (H+L) Conjugate (Promega).
-
bioRxiv - Cell Biology 2021Quote: ... 1.25 ng/L porcine trypsin (Promega, Cat No:V5280) in 25 mM NH4HCO3 was added to the gel pieces ...
-
bioRxiv - Cell Biology 2020Quote: ... 10 mmol/L EDTA (Cat. No. V4231 Promega), and 100 mmol/L NaCl (Cat ...
-
bioRxiv - Cell Biology 2021Quote: ... 2 μl) were mixed with the master mixes containing 1 μl primers (10 μM each) and reagents of GoTaq G2 (Promega #M7845) and the final volume was 25 μl ...
-
bioRxiv - Biochemistry 2024Quote: ... when the temperature was dropped to 22°C and isopropyl β-d-1-thiogalactopyranoside (IPTG) and L-rhamnose (Promega, Madison, WI) was added at 0.2 mM and 0.1% (w/v ...
-
bioRxiv - Synthetic Biology 2021Quote: ... We also included N-terminal and C-terminal HiBiT tags (Promega, Madison, WI) for quantification using luminescence ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with NOCT N-terminal mutant constructs using Fugene HD (Promega) in a ratio of 3 µL transfection reagent per 1 µg DNA ...
-
bioRxiv - Molecular Biology 2023Quote: ... HiBiT fusion vectors were constructed using the parental vector pBiT3.1-N (N2361, Promega). To generate the HiBiT-VHL vector ...
-
bioRxiv - Molecular Biology 2023Quote: ... NanoLuc fusion vectors were constructed using the parental vectors pNLF1-N (N1351, Promega) and pNLF1-C (N1361 ...
-
bioRxiv - Microbiology 2020Quote: ... 50 µ L of Kinase-Glo® reagent (Promega) was then added and incubated at room temperature for 10 minutes ...
-
bioRxiv - Molecular Biology 2021Quote: ... or goat anti-Human IgG (H+L) (Promega, W4031) antibodies were added for 30 min at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... digested using 20 ng/ l sequencing grade trypsin (Promega) for 1 hr at 47C ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: N-terminal NanoLuc/Kinase fusions were encoded in pFN31K or pFC32K expression vectors (Promega), including flexible Gly-Ser-Ser-Gly linkers between Nluc and each full-length kinase ...
-
bioRxiv - Biochemistry 2022Quote: ... N-or C-terminal NanoLuc/Kinase fusions were encoded in pFN31K expression vectors (Promega), including flexible Gly-Ser-Ser-Gly linkers between Nluc and each full-length kinase ...
-
bioRxiv - Biophysics 2021Quote: ... The primer sequence of SmBiT was designed from pBiT2.1-N [TK/SmBiT] Vector (Promega).
-
bioRxiv - Biochemistry 2023Quote: N-terminal large (LgBit) and C-terminal small (SmBit) fragments derived from NanoLuc (Promega) were fused to the C-terminus of B56δ and inserted downstream of its N-arm at residue 103 ...
-
bioRxiv - Cell Biology 2023Quote: ... and GST-N-77 WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega). Fragment purification levels were assessed by SDS-PAGE followed by Coomassie staining.
-
bioRxiv - Neuroscience 2023Quote: ... eluted in-solution samples were alkylated with N-Ethylmaleimide and digested with trypsin (Promega) using micro S-trap column (ProtiFi) ...
-
bioRxiv - Cell Biology 2024Quote: ... biological replicates (n=7) were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described (Hubner et al. ...
-
bioRxiv - Cancer Biology 2024Quote: ... were made using the NanoBRET™ PPI MCS Starter System (Promega, cat n° N1811). The RRM2 fragments were amplified by PCR using Phusion polymerase (NEB ...
-
bioRxiv - Biochemistry 2020Quote: hnRNP L and SETD2 human ORF were procured from Promega. Deletion mutants of hnRNP L and SETD2 were constructed by PCR (Phusion polymerase ...
-
bioRxiv - Immunology 2023Quote: ... 50 ☐l of Bright-Glo Luciferase reagent (Promega E2620) was added to each well ...
-
bioRxiv - Biochemistry 2021Quote: ... chymotrypsin and peptide-N-glycosidase F (PNGase F) were obtained from Promega (Madison, WI, USA). Centrifugal filters (0.5 mL ...
-
bioRxiv - Cell Biology 2019Quote: ... RT) and sequentially digested with Lys-C (Wako) (o/n at RT) and trypsin (Promega) (6 h at 37 °C) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Kanamycin-resistant colonies were selected and cultured o/n purified with Plasmid Miniprep system (Promega) and verified by sequencing (Microsynth ...
-
bioRxiv - Biochemistry 2020Quote: ... CaCl2·2H20 (0.99 g/L)) supplemented with 0.05% Tween 20 (Promega), 2.5 mM DTT ...