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Citations for Promega :
51 - 100 of 120 citations for FMOC L MEORN MTR OH since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The urea concentration was then diluted with 17 μl of 50 mM HEPES pH 8.5 and after adding 1 μl of 0.2 mg/ml trypsin (Promega) in 0.2 mM HCl the digestion continued overnight at 37°C with interval mixing at 2000 RPM for 30 s every 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with PBST and then anti-mouse IgG (H&L) alkaline phosphatase-conjugated secondary antibody (Promega) was incubated with the membrane at a 1:5,000 dilution for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: 80,000 HEK293T cells were plated in 500μl HEK Media and 24 hrs later 500ng total plasmid DNA was transfected with 1.5μl FuGene HD (Promega) as follows ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were diluted with 17 µL of 50 mM HEPES (pH 8.5) and digested with 1 µL of 0.2 mg/mL trypsin (Promega) in 0.2 mM HCl at 37°C with interval mixing at 2000 RPM for 30 sec every 15 min.
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bioRxiv - Biophysics 2022Quote: ... The poly-L-lysine coated side of the cover slip was exposed to 5 mg/ml streptavidin (Promega) in this space for 15 min ...
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bioRxiv - Microbiology 2022Quote: ... mutations were generated in a fragment of the L ORF subcloned into a pGEM T easy vector (Promega). The mutated ORF fragment was sequenced and then substituted in the L ORF of pTM1 ...
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bioRxiv - Cancer Biology 2022Quote: ... 20,000 to 50,000 cells were lysed in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 [Illumina], 10.25 μl nuclease-free water, and 0.25 μl 1% digitonin [Promega]) for 30 min at 37 °C ...
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bioRxiv - Plant Biology 2020Quote: ... 10 mg/L BSA and 1 mM PMSF) and assayed using the Dual-Luciferase® Reporter Assay System (Promega). Experiments were normalized to negative control set at 1.
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bioRxiv - Pathology 2021Quote: ... and 20 µg/mL L-012) and recorded by a Promega GloMax Navigator microplate luminometer (Promega Biotech, Beijing, China). GraphPad Prism software was used to analyze data.
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bioRxiv - Molecular Biology 2022Quote: ... and purified on a 10g/L agarose gel using the Wizard SV Gel and PCR Clean-Up system (Promega). All mixtures were made in 1x NEBuffer 3.1 on ice with final concentrations of 80nM Cas9 ...
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bioRxiv - Plant Biology 2022Quote: ... Luciferase activity of cell extracts was measured with Sirius L Tube Luminometer (Berthold) and the luciferase assay system (Promega).
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bioRxiv - Genomics 2021Quote: The supernatant was magnetically removed and the beads were resuspended in 20 µl of L3 DNA linker ligation mixture (8 µl water, 5 µl 4X ligation buffer, 1 µl RNA ligase [New England Biolabs], 0.5 µl RNasin [N2615, Promega GmbH] ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500 µl nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then diluted with 120 μL of PBS before 1.2 μL of ProteaseMax surfactant (0.1 mg/mL in 100 mM ammonium bicarbonate, Promega, V2071) and sequencing grade trypsin (10 μL ...
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bioRxiv - Cell Biology 2022Quote: ... were seeded onto 6-well plates coated with 0.1 mg ml−1 poly-L-lysine and transfected using FuGENE HD transfection reagent (Promega) with a mixture of third-generation lentiviral backbone vector pJHUMCS that encodes BDNF-pHluorin ...
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bioRxiv - Neuroscience 2023Quote: ... Then the beads were incubated with 80 μL of 2 M urea in 50 mM Tris-HCL containing 1 mM DTT and 0.4 μg trypsin (Promega) at 25 °C for 1 hour while shaking at 1,000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... 20 μL of MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) inner salt (Promega) was added to each well and the plate was further incubated for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... were supplemented with 1 µL of 1M ammonium bicarbonate (AmBic) and digested with 2 µL of 0.05 µg/µL sequencing grade trypsin (Promega) in 100 mM AmBic ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and further incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μL of lysate was diluted with 39 μL PBS before being mixed with 40 μL Dual-Glo FLuc substrate (Promega). After 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µl of the reaction was treated with 1 µl of RNase mixture (10% RNase A, Sigma + 20% RNase One, Promega), incubated at 37°C for 10 min and then mixed with 6 µl of 2 × SDS-PAGE buffer ...
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bioRxiv - Immunology 2022Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalised to a mock-treated control to generate a fold induction ...
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bioRxiv - Biochemistry 2020Quote: ... Gel pieces were cooled on ice and rehydrated with 100 μ L of 10 ng/μL Trypsin Gold (Promega, UK) for 5 min ...
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bioRxiv - Cell Biology 2021Quote: Transient transfection of A36-p14 hybrid constructs and the pE/L-LifeAct-iRFP670 expression in Vaccinia-infected cells was done using FUGENE (Promega) as described by the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and were then incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 10 μL of lysate was diluted with 30 μL PBS before being mixed with 40 μL Dual-Glo FLuc substrate (Promega). After 10 min ...
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bioRxiv - Microbiology 2020Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalized to a mock-treated control to generate a fold induction.
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bioRxiv - Molecular Biology 2021Quote: ... RNA pellets were resuspended in 8µl of DEPC water and incubated with µl (1U DNAse) and 1 µl of 10X DNAse buffer (Ref. M6101, Promega) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: The medium in 96-well plate was replaced at the indicated time points with 100 μl of PBS supplemented with 0.2 g/l EDTA and 50 μM FITC-VAD-fmk (Promega, USA) or 50 μM 1,1’,3,3,3’,3’-hexamethylindodicarbo-cyanine iodide (DiIC1(5) ...
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bioRxiv - Genomics 2021Quote: ... Membranes were washed three times in TBS-T before incubating with secondary antibodies (HRP-anti Mouse-IgG (H+L) (Promega)) for 45 min in TBS-T and 2% milk ...
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bioRxiv - Cell Biology 2020Quote: ... HRP Conjugate (W4011, 1:5000), and anti-Mouse IgG (H+L), HRP Conjugate (W4021, 1:5000) were purchased from Promega.
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bioRxiv - Microbiology 2021Quote: ... 40 µL of medium from selected wells was replaced with Nano-Glo HiBiT extracellular buffer with LgBiT and furimazine substrate (Promega). Readings were taken after a 30 min room temperature incubation as described above.
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to a concentration of urea of 2 mol L-1 and digested overnight with the addition of 2 µl sequencing-grade trypsin (Promega). After digestion ...
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bioRxiv - Immunology 2020Quote: ... Total DNA from blood and urine (around 50 μL) was extracted using a Maxwell 16 automat with the Maxwell blood DNA and cell LEV DNA purification kits (Promega), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots of culture medium (300 μl) were taken at indicated timepoints and mixed with 100 μL of 4X RealTime-Glo extracellular ATP assay reagent (Promega) reconstituted in RPMI 1640/B27(-Ins ...
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bioRxiv - Immunology 2024Quote: ... Lactate in flow-through medium was quantified using the L-lactate assay kit (Biomedical Research Service & Clinical Application, #A-108) and GloMax microplate reader (Promega) as suggested by the manufacturer ...
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bioRxiv - Bioengineering 2022Quote: ... 10 μL of the medium at several time points was collected for gLuc expression assay using Renilla Luciferase Assay System (Promega) and a luminometer ...
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bioRxiv - Developmental Biology 2024Quote: ... Total peptide concentration was adjusted to 0.15 µg/µL according to the absorbance at 280 nm against a Mass Spec-Compatible Human Protein Extract/Digest calibration curve (Promega), using the Thermo Nanodrop 1000 ND-1000 spectrophotometer (Thermo Fisher).
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bioRxiv - Molecular Biology 2023Quote: ... The final 50 µL translation reaction included the 5 µL RNA mixture described above and components from the Rabbit Reticulocyte Lysate system (Promega): 35 µL of rabbit reticulocyte lysate ...
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bioRxiv - Neuroscience 2023Quote: ... 1µl lysate was diluted with 39 µl PBS before being mixed with 40 µl of Dual-Glo FLuc substrate (Promega). After 10 min incubation ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were transfected the following day with 1,000 ng of either the control plasmid or the BFP-miR-L plasmid using Fugene HD (Promega #E2311) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Necrosis was quantified by measuring release of lactate dehydrogenase in 30 µl samples of the medium and apoptosis by determining cell-associated Caspase3/7 activity using the respective kits from Promega Corp. ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μl of the lysates were used for the quantification of Firefly and Renilla Luciferase activity using a Luminometer (GloMax, Promega).
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bioRxiv - Biochemistry 2022Quote: ... 20 μl of the lysates were used for the quantification of Firefly and Renilla Luciferase activity using a Luminometer (GloMax, Promega). The ratio between Renilla and Firefly is calculated for each experimental condition.
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed twice with 1X TBS-TT (10X TBS-TT is 10X TBS buffer with 5 mL/L Tween 20 and 5 mL/L Triton X) and once with 1X TBS before incubation with the secondary antibody (IgG anti-rabbit HRP conjugate, Promega) 1:1,000 diluted in blocking solution for 1 hr ...
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bioRxiv - Microbiology 2023Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Fold inductions were calculated by normalising to a mock-treated control.
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bioRxiv - Microbiology 2024Quote: ... and the coding region of INKV L amplified by PCR and purified using the Wizard SV Gel and PCR Clean-up System (Promega). The INKV L fragment and the pINKV-L_linker backbone were digested with Aat II and Bsr GI (New England Biolabs) ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μL of the reaction product was combined with 30 μL of a 1:50 dilution of Nano-Glo substrate (Promega) into a 364-well microplate ...
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bioRxiv - Biochemistry 2020Quote: ... Cellular debris was then pelleted by centrifugation and either 5 μl or 10 μl of the clarified supernatant used in dual luciferase assays following the manufacturer’s prescribed protocol (Promega, cat # E1910). Data are shown as the ratio of RLUC to FLUC activity averaged over six experiments.
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bioRxiv - Biochemistry 2021Quote: ... HCT116 cells were plated on poly-L-lysine coated cover slips and transfected with ER calcium sensor ER-GCaMP6-150 using FuGENE HD (Promega Corp.). Experiments were carried out 1-2 days after transfection ...