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Citations for Promega :
51 - 100 of 225 citations for CdSeTe ZnS Quantum Dots NIR region Water solvent 760nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated and the gene region of interest was amplified using GoTaq DNA polymerase (Promega) (HBB Fw ...
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bioRxiv - Microbiology 2022Quote: The intron 2 region was amplified from genomic DNA with GoTaq G2 Flexi DNA polymerase (Promega, M7801) in a final volume of 20 μl as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... regions of mtDNA were cloned from donor cell lines into p-GEM-T Easy vector (Promega, A1360). Samples were then diluted to specific concentrations and analyzed to evaluate probe specificity.
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bioRxiv - Pathology 2021Quote: ... The cDNA was diluted in nuclease-free water (Promega, USA) and used for real-time PCR with either SARS-CoV-2 or GAPDH specific primers ...
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bioRxiv - Pathology 2022Quote: ... The cDNA was diluted in nuclease-free water (Promega, USA) and used for real-time PCR with either SARS CoV-2 or 18S rRNA specific primers ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 uL of nuclease free water or RNasin (Promega, N2611). qRT-PCR reactions were set up in 10 μL volume containing 4 μL cDNA ...
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bioRxiv - Immunology 2022Quote: ... 22 μl of nuclease-free water) (#20034197, Illumina; #G9441, Promega) for incubattion at 37°C with shaking at 300 rpm for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... A non-template control consisting of nuclease free-water (Promega Corporation ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was diluted 1:1 with nuclease-free water (Promega). Quantitative real-time PCR reactions were carried out using the SYBR Green PCR master mix and ROX Passive Reference Dye (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... consisting of 13.94 µL of RNase free water (Promega, USA), 6.25 µL of master mix (Eurogentec ...
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bioRxiv - Cell Biology 2024Quote: ... 3 µl of nuclease free water (Promega, Cat No. P119E), and 5 µl of cDNA using the following program ...
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Synchronous 3D patterning of diverse CNS progenitors generates motor neurons of broad axial identitybioRxiv - Neuroscience 2024Quote: ... Double elution with 20- 30µl of Nuclease-free Water (Promega) was performed ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription (RT) of different HCV genomic regions was performed using avian myeloblastosis virus (AMV) reverse transcriptase (Promega), and subsequent PCR amplification was carried out using AccuScript (Agilent Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... entire gel lanes were excised into 5 equal regions from top to bottom and digested with trypsin (Promega) as described (30) ...
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bioRxiv - Cancer Biology 2021Quote: The upstream 376 bp region of the human LGALS1 transcriptional start site was cloned into the pGL4.23 (Promega) vector to generate the LGALS1 luciferase reporter gene (LGALS1 pGL4.23 ...
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bioRxiv - Molecular Biology 2022Quote: ... the GST region of psfRps29-GST was replaced with luciferase cDNA (pNL1.1[Nluc]Vector; Promega Madison, WI, USA). Luciferase cDNA was amplified via PCR using primers Nluc_FW2 and Nluc_RV2 ...
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bioRxiv - Developmental Biology 2022Quote: CEBPa promoter (−2kb) or enhancer region (Cooper et al., 2015) was cloned into the pGL3 basic backbone (Promega). The construct containing part of the Id1 promoter region (336 bp ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR products from the unique regions of her9 were cloned into the pGEMT-easy vector (Promega, Madison, WI). Plasmids were linearized using either NcoI and SpeI restriction enzymes (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... pGL3basic-IFNβ-Luc (mIfnb1- FLuc) consists of the 812 bp murine Ifnb1 promoter region cloned into pGL3basic (Promega) upstream of the firefly luciferase gene (99) ...
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bioRxiv - Molecular Biology 2024Quote: ... the genomic region including each target site was amplified by PCR using GoTaq Hot Start polymerase (Promega, # M5001). PCR reactions contained 400 ng of gDNA and 0.75 μM of the corresponding primers in a total volume of 30 μl ...
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bioRxiv - Cell Biology 2020Quote: ... the artificially synthesized PlGF 3’ untranslated regions (UTR) gene fragment was constructed into pMIR-reporter (Promega, Madison, WI, USA). A complementary sequence with mutation of the seed sequence was designed based on the wild type (WT ...
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bioRxiv - Molecular Biology 2021Quote: ... a region of the COX1 gene was amplified by PCR using GoTaq® G2 Green Master Mix (Promega, Australia) and primer set LepF (5’-TTCAACCAATCATAAAGATATTGG-3’ ...
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bioRxiv - Cell Biology 2021Quote: ... fumigatus pyrGAf gene and 870 nucleotides of the myoE 3’-UTR region was cloned in pGEM-T easy (Promega). This plasmid was used as template for site-directed mutagenesis (QuickChange kit ...
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bioRxiv - Plant Biology 2021Quote: ... the full length of the native GmDGAT1A coding region was cloned into a pGEMR-T Easy vector (Promega, USA). Site-directed mutagenesis was performed by inverse PCR using PrimeSTAR GXL polymerase (Takara ...
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bioRxiv - Genomics 2022Quote: Approximately 500-bp regions of DNA containing rs80282103 and rs11154336 were amplified from purified human genomic DNA (Promega, #G1521) by PCR using engineered restriction sites to allow directional cloning into the multiple cloning region of the pGL4.23[luc2/minP] luciferase reporter vector (Promega ...
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bioRxiv - Neuroscience 2021Quote: We constructed luciferase reporter plasmids by cloning an ∼900 bp region containing human 1b30 into the pGL4.24 vector (Promega) upstream of the minP ...
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bioRxiv - Neuroscience 2021Quote: Luciferase reporter constructs were constructed by cloning a candidate enhancer region into the pGL3 promoter vector (Promega; Madison, WI). Each region was inserted using standard restriction enzyme-based cloning techniques ...
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bioRxiv - Cell Biology 2022Quote: ... Regions of interest were amplified in a PCR reaction with GoTaq G2 Flexi DNA polymerase (Promega, Madison, WI, USA), and PCR-amplified samples were run on a 1,2% TBE-agarose gel for size separation ...
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bioRxiv - Neuroscience 2022Quote: Luciferase reporter constructs were generated by cloning a candidate enhancer region into the pGL3 promoter vector (Promega; Madison, WI). Each region was inserted using standard restriction enzyme-based cloning techniques ...
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bioRxiv - Molecular Biology 2024Quote: ... and the equivalent region in the human (−207/+5) were cloned into pGL3-basic Luciferase reporter-vector (E1751, Promega) using the forward primer containing XhoI restriction site ...
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bioRxiv - Genetics 2024Quote: ... Existing plasmids constructs of variant-centered test elements for the five variants of interest (see Dataset S1 for genomic regions) cloned in the pGL4.23 vector (Promega) were used as the initial template in PCR ...
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bioRxiv - Neuroscience 2023Quote: ... A 9.5 kb fragment containing upstream promoter region of mouse Frizzled10 (Fzd10) gene was subcloned into pGEM vector (Promega). CreERT2gene (Feil et al. ...
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bioRxiv - Immunology 2023Quote: Promoter constructs were created by cloning the immediate 4.5-kb region adjacent to the 5’ TSS of SPINK7 into the promoterless Nano-luciferase reporter vector pNL1.1-NL (Promega). The 4.5-kb sequence and subsequent constructs were created by using primers with the restriction enzyme sites KpnI-HF and XhoI ...
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bioRxiv - Immunology 2023Quote: HIC1 binding on RUNX1 promoter was assessed using Dual-Luciferase® Reporter Assay by cloning the ChIP-defined genomic region upstream of a minimal promoter driving a luciferase gene (pGL4.10 [luc2/minP]; Promega). Wild type HIC1 binding motif of the RUNX1 proximal promoter (5′-TGCCCTGG-3′ ...
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bioRxiv - Neuroscience 2023Quote: ... We amplified the regions from C57Bl/6 tail snip DNA or from human male genomic DNA (Promega catalog # G1471) using FastPhusion 2x Master Mix (Thermo Fisher catalog # F548L) ...
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bioRxiv - Plant Biology 2023Quote: ... gRNA-targeted regions were amplified from genomic DNA isolated from the T1 plants with GoTaq Green Master Mix (Promega) using the primers g1-seqF/g1-seqR and g2-seqF/g2-seqR for gRNA1 and gRNA2 ...
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bioRxiv - Developmental Biology 2024Quote: ... containing the promoter region of spia-1 (1.23 kb upstream from the start ATG) and the entire genomic region into the pGEMT vector (Promega). A transcriptional construct (pSO22) ...
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bioRxiv - Physiology 2024Quote: The enhancer region containing the FXR binding element was amplified by PCR and cloned into the pGL4.23 vector (Promega). HepG2 cells were transiently transfected with FXR (100 ng/well ...
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bioRxiv - Physiology 2020Quote: ... and eluted into 20 μl of nuclease- free water (P1195; Promega). All libraries were prepared from 100 to 200 pg of cDNA using the Nextera XT Kit (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... freshly add 3 µL 1:1 water diluted digitonin (Promega G9441)) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... 19.5µl of nuclease-free water) (FC-121-1030, Illumina; G9441, Promega). Transposition reactions were incubated at 37°C for 30 minutes in an Eppendorf ThermoMixer with agitation at 1000 RPM ...
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bioRxiv - Microbiology 2024Quote: ... 4,7 μL of PCR amplification-grade water (Promega Corporation, Wisconsin, USA) and 2 μL of sample DNA (about 20 ng) ...
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bioRxiv - Cell Biology 2024Quote: ... 22 μl of nuclease-free water) (Illumina, #20034197 and Promega, #G9441) at 37°C for 30 minutes with 300 rpm shaking ...
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bioRxiv - Cell Biology 2024Quote: ... 30 µL of CellTiter-Glo (Promega, diluted 1:6 in water), was added to each well and luminescence was measured with an Envision plate reader.
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bioRxiv - Plant Biology 2024Quote: ... DNA was dissolved in milliQ water and treated with RNase (Promega) for 30 minutes at 37 ◦C.
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bioRxiv - Microbiology 2021Quote: Promoter fusions to lacZ were constructed by amplification of promoter regions from genomic DNA with the specified primers (Table S4) and ligated into pGEM-T Easy (Promega) for amplification and collection ...
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bioRxiv - Developmental Biology 2021Quote: ... the proximal promoter region (−299/−1) of MyoG was isolated by PCR and inserted into the pGL3-basic vector (Promega). The DNA fragments of PME1 and PME2 in the MyoG promoter region were also amplified and inserted into the pGL3-γ-actin-TATA vector ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic regions flanking the CRISPR target sites were PCR amplified with designed primers (Supplemental Table 3) using GoTaq DNA polymerase (Promega) and sent for Sanger sequencing to determine the insertion and deletion errors generated by CRISPR-Cas9 system in exon 2 of PARKIN ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.25 µg of DNA was used to amplify specific regions of Trp53 and Nf1 gene using Go-Taq DNA Polymerase (Promega). The primers sequences are summarized in Suppl ...
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bioRxiv - Microbiology 2022Quote: ... Both cassettes were built by first cloning the wild-type locus and 1 kb flanking region in the pGEM-T plasmid (Promega) and subsequently modifying the plasmid to add the HA-tag coding sequence using the Q5 Site-Directed Mutagenesis kit (NEB ...