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Citations for Promega :
51 - 100 of 4227 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 20 μL of MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) inner salt (Promega) was added to each well and the plate was further incubated for 1 h at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were added to each well and co-cultured for 16-20 hr before addition of Bright-Glo™ (Promega) lysis buffer and luciferin substrate ...
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bioRxiv - Biochemistry 2021Quote: ... and 20 μL/well of a 1:20 dilution of BrightGlo (Promega,) in assay buffer supplemented with 0.01 % pluronic F68 (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: ... Apoptosis was assessed by incubating the cells with 200nM Staurosporin or medium for 4 h and the caspase 3/7 activity was assayed using the ApoOne Caspase 3/7 Assay (Promega). Senescence associated β-Galactosidase activity was analyzed with the Senescence Associated β-Galactosidase Staining Kit (Cell Signalling Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... These cells were transfected with the pcDNA6.2-GW /EmGFPmiR plasmids containing miR sequences (miR-NRF2 #1, #2, #3, #4 and miR-ctl 79 using the FUGENE HD transfection reagent (Promega #E2311). Seven days later GFP positive cells were isolated by flow cytometry (Biorad S3e cell sorter) ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested from Calu-3 cells and inactivated by addition of lysis buffer (Maxwell 16 viral total nucleic acid purification kit, Promega #AS1150) complemented with proteinase K ...
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bioRxiv - Microbiology 2020Quote: ... Twenty nanograms of DNA were used as a model for 18 cycles of amplification of the V4 region of the 16S rRNA gene, using primers 515F and 806R (Caporaso, et al. 2012: 1621-4) and GoTaq Master Mix (Promega). PCR products were quantified with the Qschd dsDNA HS kit (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligos were then ligated at 4°C overnight to the appropriate digested ACR-16-REM sequence (T4 ligase, Promega, USA) and transformed into DH5α (ThermoScientific ...
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bioRxiv - Systems Biology 2023Quote: ... 4 μL of digestion buffer containing 20 ng/μL sequencing-grade trypsin (Promega), 20 ng/μL sequencing-grade Lys-C (Wako) ...
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bioRxiv - Cell Biology 2022Quote: ... and Maxwell 16 Instrument (Promega) were used to isolate RNA from mouse PCLS according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... using the Maxwell 16 (Promega) utilizing magnetic nickel beads in a semi-automated nickel affinity purification strategy ...
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bioRxiv - Microbiology 2022Quote: ... The samples were incubated at 30 °C and luminescence was quantified after 2 h and 4 h post infection (20 μl sample plus 20 μl substrate, Nano-Glo® Luciferase Assay System, Promega). The sample was carefully removed from the upper part of the tube without disturbing the sedimented erythrocytes in the blood samples.
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bioRxiv - Developmental Biology 2021Quote: ... and 175 μg/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Promega). Reactions were stopped with three PBS rinses ...
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bioRxiv - Developmental Biology 2022Quote: ... and 175 μg/ml 5-bromo-4-chloro-3- indolyl-phosphate (BCIP) (Promega). Reactions were stopped as the signal became apparent with three PBS rinses ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following day cells were trypsinized and seeded in 384-well white plate (20 µl/well) in DMEM F12 (no phenol red, 4% FBS) +/-HaloTag® NanoBRET™ 618 Ligand (1 µl/ml, Promega) and +/-compounds (DMSO concentration in each sample was kept the same) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... CellTiter 96 AQueous One Non-Radiactive Cell Proliferation Assay based on 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) was from Promega (Duebendorf, Switzerland). COmplete™ EDTA-free protease inhibitor cocktail was obtained from Roche Diagnostics (Mannheim ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µL of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) reagent (#G3582; Promega, Madison, WI) was added and incubated at 37°C for 45 mins ...
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bioRxiv - Cell Biology 2022Quote: ... anti-goat alkaline phosphatase-linked antibody and alkaline phosphatase substrate (5-bromo-4-choloro-3-indolyl 1-phosphate and nitroblue tetrazolium) from Promega (Southampton, UK); a hydroxamate-based MMP inhibitor CT-1746 (N1-[2-(S)-(3,3-dimethylbutanamidyl)]-N4-hydroxy-2-(R)-[3-(4-chlorophenyl)-propyl]-succinamide ...
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bioRxiv - Genomics 2020Quote: ... 4 µL RNase-A (4 mg-mL-1, Promega, Madison WI) was incubated at 37 °C for one hour vortexing every 15 minutes ...
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bioRxiv - Biophysics 2019Quote: ... NanoLuc substrates (Promega, after 1:20 dilution) were added at 10 μl/well followed by incubation by 10 μl of 4×compound solutions for 15 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and LgBiT (1:20 dilution; #N2421, Promega) was added 20 min after the last WNT-3A addition and the plate was incubated for 10 min at 37 °C without CO2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and LgBiT (1:20 dilution; #N2421, Promega) were added ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... after which growth media was removed and 20 µl of 4% D-luciferin (Promega) in CO2 independent medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... and 9 μl Fugene HD transfection reagent (Promega) was incubated for 10 minutes at room temperature and transfected into each well ...
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bioRxiv - Cell Biology 2019Quote: ... and 9 µl Fugene HD transfection reagent (Promega) was incubated for 10 minutes at room temperature and transfected into each well ...
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bioRxiv - Molecular Biology 2021Quote: ... 11 μL of substrate mix (10 μL of Promega Nano-Glo® Luciferase Assay Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and 20 µL of 1:1000 (diluted in phenyl red-free DMEM with 4% FBS) HaloTag™ NanoBRET™ 618 Ligand (Promega G9801) was added to each well ...
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bioRxiv - Cell Biology 2021Quote: ... 16 nmol of unlabelled dTTP (Promega) and 30 units of terminal deoxynucleotidyl transferase (Promega ...
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bioRxiv - Plant Biology 2021Quote: ... acaule with a Maxwell 16 Instrument and Maxwell 16 Tissue DNA Purification Kits (Promega, Madison, WI, USA). SMRT sequence libraries were constructed with an SMRTbell Express Template Prep Kit (PacBio ...
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bioRxiv - Biochemistry 2023Quote: ... MTS ([3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (#G3581) was purchased from Promega (Charbonnières-les-Bains, France).
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bioRxiv - Molecular Biology 2020Quote: ... Resuspended grindate was incubated (4°C, 20 min) with 660 U of DNase I (Promega). Insoluble cell debris was pelleted by spinning (16,000 g ...
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bioRxiv - Cell Biology 2021Quote: ... 20 U SP6 RNA polymerase (Promega; 1 µl). Following a 1-h incubation of the cocktail at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... was transfected at a ratio of 9:1 for the candidate enhancer:constitutive promoter driving Renilla luciferase (pGL4.75, Promega). Luciferase activity was determined using the Promega Dual-Luciferase® Reporter Assay System according to manufacturer specifications on a Synergy HI plate reader (Bio-Tek).
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bioRxiv - Developmental Biology 2022Quote: ... freshly add 3 µL 1:1 water diluted digitonin (Promega G9441)) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... and luminescence-based caspase-glo 9 assay system (Promega), respectively ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... consisting of 9 µg/mL of carrier DNA (Promega) and 1 µg/mL of CSNK2A-NLuc fusion DNA in Opti-MEM without serum (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... consisting of 9 µg/mL of carrier DNA (Promega) and 1 µg/mL of CSNK2A2-NLuc fusion DNA in Opti-MEM without serum (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were seeded on 24 mm glass coverslips and transfected with 2 µg plasmid DNA (1:1.3:3 ratio LAMP1:ER:opto-kinesin) and Fugene6 transfection reagent (Promega 1:3) for 20-30 h prior to imaging ...
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bioRxiv - Neuroscience 2023Quote: ... urea concentration for each sample was diluted to <1 M using ammonium bicarbonate (ABC) and digested for an additional ∼16 hours using 1:50 (w/w) trypsin (Promega). Following digestion ...
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bioRxiv - Bioengineering 2023Quote: ... mixed with 0.1 volume of 3M Sodium Acetate (Promega, UK) and 1 volume of isopropanol (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... run on the Maxwell 16 Instrument (Promega) and/or mirVana miRNA Isolation Kit (Invitrogen by Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... on a Maxwell RSC 16 instrument (Promega) after homogenisation with glass beads ...
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bioRxiv - Plant Biology 2019Quote: ... in the Maxwell 16 AS2000 instrument (Promega), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... using a Maxwell 16 MDx machine (Promega). RNA quality and concentration were assayed using a BioTek Epoch Microplate Spectrophotometer ...
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bioRxiv - Molecular Biology 2020Quote: ... and Maxwell 16 Automated Purification System (Promega). The concentration of RNA was measured using QuantiFluor RNA System (Promega ...
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bioRxiv - Microbiology 2023Quote: ... in a Maxwell 16 MDx instrument (Promega). After purification ...
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bioRxiv - Cancer Biology 2024Quote: ... on a Maxwell® 16 MDx (Promega). RNF43 exome sequencing was performed using a RNF43 Custom Panel (Illumina) ...
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bioRxiv - Biochemistry 2023Quote: ... HRP (4 μM) was added followed by 20 μL of Nano-glo luciferase substrate (Promega, N1110) to each well ...
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bioRxiv - Microbiology 2021Quote: ... The 1/10 of the sample was kept for injection and the 9/10 were digested with trypsin (Promega) (12.5 ng/µl ...
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bioRxiv - Biochemistry 2022Quote: ... 1.7 μg of attB-hACE2-mNG2-1-10 plasmid or attB-S-mNG2-11 plasmid were added into 5 μL FuGENE 6 transfection reagent (Promega) and OptiMEM (Gibco ...