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Citations for Promega :
51 - 86 of 86 citations for 18 0 PE square since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... One μg of RNA was reverse-transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega). Quantitative real-time PCR (qPCR ...
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bioRxiv - Microbiology 2021Quote: IL-18 truncations were generated through in vitro transcription/translation using pET-pro-IL-18 as a template in 10 µl reaction volumes following the manufacturer’s recommendations (TNT Coupled Reticulocyte Lysate; Promega) as previously [40] ...
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bioRxiv - Microbiology 2022Quote: ... Luminescence readings were taken at 0 and 72 h post-invasion using the Renilla-Glo® Luciferase Assay System (Promega cat. E2750) and the GloMax® Discover Multimode Microplate Reader (Promega) ...
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bioRxiv - Biochemistry 2022Quote: HeLa and HEK293T cells were seeded on 18 mm glass coverslips at a density of 3×105 cells/mL and were transfected with FuGENE 6 (Promega) transfection reagent 24 h later ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed using 2μg RNA of each sample and oligo (dT)18 primers by M-MLV reverse transcriptase (Promega, 28025013). Three independent biological samples were collected and analyzed ...
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bioRxiv - Plant Biology 2021Quote: ... One microgram of RNA was reverse transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega, https://worldwide.promega.com/). Quantitative real time PCR (qPCR ...
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bioRxiv - Biochemistry 2022Quote: ... Infected cells were harvested at 18 hpi and luciferase activity was measured using a luminometer-Glomax 20/20 (Promega, USA).
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bioRxiv - Microbiology 2022Quote: ... Infected cells were incubated for approximately 18-20 hours and luciferase was measured using the Promega BrightGlo luciferase kit following manufacturers’ instructions (Promega). Entry signal was normalized to the average signal for the “no spike” control ...
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bioRxiv - Microbiology 2021Quote: ... The virus-serum mix was subsequently used to infect A549-hACE2-TMPRSS2 cells7 for 18 hours at 37 °C before adding ONE-Glo reagent (Promega) for measurement of the luciferase signal by relative luminescence units (RLUs) ...
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bioRxiv - Microbiology 2021Quote: ... The virus-serum mix was subsequently used to infect A549-hACE2-TMPRSS2 cells [41] for 18 hours at 37°C before adding ONE-Glo reagent (Promega) for measurement of the luciferase signal by relative luminescence units (RLUs) ...
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bioRxiv - Systems Biology 2021Quote: ... In 18 hours cells were stimulated for 8 hours with a corresponding inducer and harvested in the passive lysis buffer (Promega). Luminescence of Firefly and Renilla luciferases was measured using dual-luciferase-reporter assay (Promega ...
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bioRxiv - Immunology 2021Quote: ... luminescence was measured at 18 hours post-treatment with untreated samples included as a control (GloMax Discovery, Promega, Madison, WI). Data was normalized to the untreated control and presented as the average (N=3 ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by a DNase I treatment and a subsequent reverse transcription with oligo d[T]18 using the GoScript RT kit (Promega). One-step RT-qPCR was performed using the SYBR FAST Mix optimized for LightCycler 480 (KAPA ...
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bioRxiv - Plant Biology 2022Quote: ... Protein precipitates were resolubilized in 5% trifluoroethanol of aqueous 100 mM TEAB and digested overnight (18 hours) with a Trypsin+LysC mixture (Promega) at a protein to protease ratio of 25:1 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a total of 18 RNA extractions (3 morphs × 3 tissues × 2 biological replicates) were performed using the SV Total RNA Isolation System (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Luciferase activity was determined 18-24 hr post-transfection from cell lysate using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturers protocols ...
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bioRxiv - Immunology 2023Quote: ... pI.18-3xFlag-myRIG-I or pI.18-3xFlag-roRIG-I (25 ng/well each) together with transfection control pLR-SV40-Renilla (Promega) (50 ng/well ...
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bioRxiv - Cancer Biology 2023Quote: ... The system was incubated for 16-18 hours followed by addition (equivolumetric ratio) of Bio-Glo Luciferase Assay Reagent (Promega) at RT ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Protein precipitates were resolubilized in 5% trifluoroethanol of aqueous 100 mM TEAB and digested for 18 h with Trypsin+LysC (Promega) at a ratio of 25:1 protein:protease ...
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bioRxiv - Plant Biology 2024Quote: ... Transfected cells were cultured for 16-18 h followed by analysis of relative luciferase (LUC) activity using the Dual-Luciferase Reporter Assay System (Promega), which detects the activities of firefly LUC and the internal control Renilla reniformis (REN) ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was performed on 1 μg total RNA using an oligo (dT)18 primer and MMLV reverse transcriptase (Promega). For qPCR ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Immunology 2021Quote: ... The virus-serum mix was subsequently used to infect A549-hACE2-TMPRSS2 cells for 18 hr at 37 Celsius before adding ONE-Glo reagent (Promega E6120) for measurement of luciferase signal (relative luminescence unit ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA (1 μg) was reverse-transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega, Fitchburg, WI, USA).
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bioRxiv - Plant Biology 2022Quote: ... Total RNA (1 μg) was reverse-transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega, Fitchburg, WI, USA).
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bioRxiv - Microbiology 2022Quote: ... Transfection was achieved by adding 6 μg of plasmid DNA in 18 microliters of FuGENE® 6 Transfection Reagent (Promega # E2691); the obtained mixture was incubated in plain media for 20 minutes and then added to the pre-seeded culture dishes ...
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bioRxiv - Microbiology 2024Quote: ... copy of the 16S rRNA gene of Bacillus paralicheniformis and a copy of the 18S rRNA gene of Aspergillus fumigatus were separately cloned using the pGEM®-T Easy Vector System (Promega). Positive clones were selected and subsequently grown overnight ...
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bioRxiv - Evolutionary Biology 2024Quote: ... fruticulosa plants hydroponically cultivated under 0 mM or 150 mM NaCl was extracted using the Maxwell plant RNA kit (Promega Corporation, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were incubated with chemical treatments for 18 hours at 37°C prior to rinsing with PBS and lysing with 1x cell lysis buffer (Promega, Madison, WI). Luciferase activity was measured using a Berthold Centro XS3 LB 960 microplate luminometer with automatic injection of Luciferase Assay Reagent (Promega) ...
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bioRxiv - Genomics 2024Quote: ... Both obtained telomeric DNA and 18S ribosomal DNA (rDNA) were then purified by Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) and quantified by Qubit 3.0 fluorometer.
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bioRxiv - Cell Biology 2020Quote: ... GFP-MICAL1 and GFP-MICAL2 stable cells were transfected with pE/L-LifeAct-iRFP670 using FUGENE (Promega) and infected with vaccinia virus as previously described (35) ...
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bioRxiv - Cancer Biology 2024Quote: ... Each qPCR reaction contained 2 µL cDNA sample (12.5 ng) and 18 µL mastermix with 1X GoTaq® qPCR Master Mix (Promega, Charbonnières les Bains, France) and 0.5 µM primer ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was measured by MTS/PES One Solution Cell Titer Assay following manufacturer’s protocol (Promega Corp, Madison, WI).
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bioRxiv - Neuroscience 2024Quote: ... and Gsx2-PE near the Gsx2 gene were created by PCR and subsequently cloned into the pGL4.23 firefly luciferase vector (Promega) upstream (U ...
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bioRxiv - Cell Biology 2021Quote: Transient transfection of A36-p14 hybrid constructs and the pE/L-LifeAct-iRFP670 expression in Vaccinia-infected cells was done using FUGENE (Promega) as described by the manufacturer ...