Labshake search
Citations for Promega :
901 - 950 of 5334 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2019Quote: ... Luminescence was measured for 1 sec every 1 min at room temperature using GloMax-Multi+Detection System (Promega). ROS production was expressed in relative luminescence units (RLU).
-
bioRxiv - Microbiology 2020Quote: ... and 24 hours after media changing the infection was quantified by luciferase detection with BrightGlo luciferase assay (Promega) and read in a Victor3 plate reader (Perkin Elmer ...
-
bioRxiv - Cell Biology 2021Quote: ... and the amount of the HiBiT protein was measured using the Nano Glo HiBiT Lytic Detection System (Promega). As a quantification standard for HiBiT proteins ...
-
bioRxiv - Neuroscience 2022Quote: ... samples were prepared in a 96-well plate for luminescence measurement using the GloMax-Multi Detection System (Promega). For data analysis ...
-
bioRxiv - Synthetic Biology 2020Quote: Expression from stably-integrated HiBit:GUS:YFP transgenes was quantified using the Nano-Glo® HiBiT Extracellular Detection System (Promega). 10 mg leaf tissue was homogenised in 50 μL passive lysis buffer (Promega ...
-
bioRxiv - Microbiology 2023Quote: ... The size of any positive IS2404 detection was assessed against 2 µL of 100 bp DNA Ladder (Promega) and run at 50 V for 1.5 hrs before being visualised with an EZ Gel Documentation System (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of ADP Glo reagent then kinase detection reagent as specified by the manufacturer (Promega). Luminescence was measured in a 384-well plate using a TECAN Infinite M1000 microplate reader (Mannedorf ...
-
bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesized using Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and universal Random hexamers (Promega). qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed on 1 μg of RNA with random hexamer primers using MMLV-RT enzyme (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... Reverse transcription (RT) of different HCV genomic regions was performed using avian myeloblastosis virus (AMV) reverse transcriptase (Promega), and subsequent PCR amplification was carried out using AccuScript (Agilent Technologies) ...
-
bioRxiv - Plant Biology 2021Quote: ... One microgram of total RNA was reverse transcribed using an oligo(dT)15 and the MMLV-RT (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 µg of total RNA to cDNA using Oligo(dT)15 primers (Promega) ...
-
bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
-
bioRxiv - Physiology 2021Quote: ... The resultant cDNA was assessed by RT-qPCR using SYBR® Green Supermix 2x qPCR master mix (Promega) in a ABI PRISM 7900HT Real-Time PCR system (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:50 ...
-
bioRxiv - Microbiology 2022Quote: ... The reverse transcription (RT) mix contained 200 units of recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Promega), 20 units of RNAsin (Promega) ...
-
bioRxiv - Molecular Biology 2023Quote: A total of 10 µg RNA was taken from each sample for reverse transcription (RT) using GoScript (Promega) reverse transcriptase (5 µg for RT ...
-
bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was analyzed by RT-qPCR using SYBR green fluorescent dye 2x qPCR master mix (Promega) in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and the housekeeper gene Hprt (ID: Mm03024075_m1) using GoTaq Probe qPCR and RT-qPCR System (Promega, Madison, WI) and Quantstudio 7 flex RT-PCR system (ThermoFisher Scientific) ...
-
bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis and qPCR were performed using the GoTaq 2-step RT-qPCR system (Promega, Madison, MA, USA) with an ABI Prism 7900HT Sequence Detection System (Applied Biosystems ...
-
Identification of echinacoside as a tobramycin potentiator against Pseudomonas aeruginosa aggregatesbioRxiv - Microbiology 2024Quote: ... Such cDNA samples were applied as templates for RT-qPCR using 2× GoTaq® qPCR Master Mix (Promega). The reactions were performed on the CFX96TM system (BIO-RAD ...
-
bioRxiv - Physiology 2024Quote: ... The resulting cDNA was analyzed by RT-qPCR using SYBR green fluorescent dye 2x qPCR master mix (Promega) in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were tested at each time point using CellTiter Glo (Promega, Cat #G7570), which measures ATP production ...
-
bioRxiv - Cell Biology 2019Quote: Doubling times were measured using the CellTiter-Glo Luminescent Cell Viability Assay (Promega) using manufacturer recommendation ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cell viability at each time point was assayed with Cell Titer glo (Promega) according to the manufacturer’s protocol.
-
bioRxiv - Plant Biology 2020Quote: ... The positive PCR products were cleaned using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI) and submitted for Sanger sequencing at Roy J ...
-
bioRxiv - Developmental Biology 2021Quote: ... The resulting cDNA was subjected to PCR or qPCR analysis using Phire Green Hot Start II PCR Master Mix (Promega) or GoTaq qPCR Master Mix (Promega) ...
-
bioRxiv - Neuroscience 2020Quote: ... The PCR products were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Wisconsin, USA, A9281) and annealed (denaturation at 95°C for 5 min and then slow cooling of the samples at the rate of -2°C/sec from 95°C to 85°C and then - 0.1°C/sec from 85°C to 25°C for annealing of heteroduplexes ...
-
bioRxiv - Biochemistry 2021Quote: ... Both PCR products were gel-purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Mannheim, Germany) and assembled via isothermal in vitro ligation [37] ...
-
bioRxiv - Microbiology 2019Quote: ... Products of PCR reactions were purified using the Wizard® SV Gel and PCR Clean-Up system (Promega, Madison, USA). Sequencing reactions were performed using the Big-DyeTM Terminator Version 3.1 Ready Reaction Cycle Sequencing Kit (Applied BioSystems ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR product was amplified with Phusion HotStart II DNA polymerase and the PCR fragment was ligated with T4 ligase (Promega) and transformed into Novablue competent cells ...
-
bioRxiv - Microbiology 2021Quote: ... PCR amplicons were gel purified using the Wizard® SV Gel and PCR Clean-Up System (Promega Cat. No. A9281). Next ...
-
bioRxiv - Genomics 2019Quote: ... PCR products run on 1% agarose gel and purified using Wizard® SV Gel and PCR Clean-Up System (Promega). Purified PCR products were sequenced and analyzed with the Sequence Scanner Software 2 Version 2 (Applied Biosystems).
-
bioRxiv - Evolutionary Biology 2021Quote: ... PCR product was purified with the Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA), quantified ...
-
bioRxiv - Cell Biology 2021Quote: ... DNA fragments containing the T7 promoter sequence were further amplified by PCR and purified using a Wizard SV Gel and PCR clean-up system (#A9281, Promega) to prepare templates for in vitro transcription ...
-
bioRxiv - Plant Biology 2022Quote: ... Quantitative reverse transcription PCR (qRT-PCR) was performed according to the method of Ehira and Miyazaki43 using Go Taq qPCR Master Mix (Promega). Primers used for PCR and qRT-PCR are listed in Supplementary Table 5.
-
bioRxiv - Synthetic Biology 2023Quote: ... while the purification of the PCR fragments was performed using the Wizard SV Gel and PCR Clean-Up System (Promega).
-
bioRxiv - Plant Biology 2023Quote: ... PCR reactions were conducted in 10 μL volume comprising 5 μL Promega 2x PCR Master Mix (Promega, Madison, Wisconsin, USA), 10 pmol of forward and reverse primers ...
-
bioRxiv - Immunology 2021Quote: ... 400 µL PCR products were purified by Promega Wizard Gel/PCR purification columns ...
-
bioRxiv - Developmental Biology 2020Quote: ... The PCR product was gel extracted (Promega, A9281). Wildtype embryos were injected with a mix containing two sgRNAs (56.25 ng/μl each) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 μl 10 mM PCR dNTP mix (Promega) and 0.5 μl RNase free water ...
-
bioRxiv - Zoology 2021Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 μl MgCl ...
-
bioRxiv - Cell Biology 2021Quote: ... a PCR was done using Taq polymerase (Promega) and visualize on agarose gel to assess xbp1 ...
-
bioRxiv - Neuroscience 2022Quote: ... PCR was performed using Go Green Taq (Promega) according to the vendor protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... PCR products were ligated to pGEM-T (Promega) or pBluescript SKII (− ...
-
Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... Colony PCR was performed using GoTaq polymerase (Promega). The ΔhilE hilD::mCherry was constructed by incorporating hilD::mCherry construct into the ΔhilE strain.
-
bioRxiv - Biophysics 2022Quote: ... the two purified flanking PCR fragments (Promega, USA) were assembled with BamHI and HindIII-digested PK18 (Gmr ...
-
bioRxiv - Microbiology 2022Quote: ... Diagnostic PCR was performed using GoTaq polymerase (Promega) or OneTaq DNA polymerase.
-
bioRxiv - Cancer Biology 2022Quote: ... PCR was performed in GoTaq Master Mix (Promega) according to standard procedures ...