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Citations for Promega :
851 - 900 of 1480 citations for Human PLA2G4F shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The HaloTag-SmBiT fragment was PCR amplified from the commercially available plasmid (Promega, Cat no: N202A) and also subcloned into pcDNA3.1 ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were co-transfected with the BeGC1 plasmid and the pGloSensor-42F cGMP vector (Promega) using Lipofectamine 2000 (Thermo Fischer Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... The linearized plasmids were used as templates for in vitro transcription with T3 RNA polymerase (Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was combined at a 3:1 ratio of FuGENE® 6 Transfection Reagent (Promega) to DNA and incubated 20 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... BT-549 and HCC38 cells were transfected with both plasmids simultaneously (2 μg) using FuGene (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... plasmids at the designated concentrations were added to OptiMEM media with FuGENE HD Transfection Reagent (Promega) at a 3:1 FuGENE:DNA ratio ...
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bioRxiv - Cancer Biology 2022Quote: ... and NUMB1-4 (from V.O.R.) expression plasmids was performed using FuGENE HD transfection reagent (Promega, E2311) according to the manufacturer’s protocol and selected with Geneticin (Gibco ...
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bioRxiv - Plant Biology 2019Quote: ... we used a control plasmid provided in the TnT® Quick Coupled Transcription/Translation System (Promega). The plasmid was linearized using the restriction enzyme SacI and purified using the DNA Clean and Concentrator Kit (Zymo Research) ...
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bioRxiv - Immunology 2020Quote: ... All luciferase constructs were co-transfected with pRL-TK Renilla luciferase plasmid (Promega, Madison, WI, USA) to normalize transfection efficiency variation between samples ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 ng of donor and gRNA plasmids in 1:1 ratio was used using ViaFect (Promega) transfection reagent as per manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... The pTM plasmids were transfected into H7-T7-IZ cells using ViaFect™ Transfection Reagent (Promega) following the manufacturer’s recommendations.
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bioRxiv - Molecular Biology 2020Quote: Aag2 and U4.4 cells were transfected with the appropriate plasmids using Fugene HD Transfection Reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plasmids encoding the cAMP sensor (pGloSensor-22F) and the following luciferase reporters were purchased from Promega: CRE-luc2 ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA of GRXS17 (At4g04950) were inserted into the pGEM-T Easy plasmid (Promega, Madison, WI, USA). Different point mutations of cysteines to serines in GRXS17 were generated using QuikChange II Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Immunology 2021Quote: ... cells were transfected with the plasmid of the required sensor using FuGene HD transfection reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... They were transfected with multiple plasmids at an amount of 50 ng each using FugeneHD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The 1 μg linearized plasmid was serving as a template for in vitro transcription (SP6, Promega), and the products was then transfected into cells with Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... pMAD plasmid was also digested with MluI and NcoI and dephosphorylated by addition of TSAP (Promega, streamlined restriction digestion protocol ...
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bioRxiv - Immunology 2021Quote: ... HEK 293T cells were transfected with the pseudovirus encoding plasmids using FuGENE 6 Transfection Reagent (Promega). The virus culture supernatant was harvested at 48h and 72h post transfection and stored at -80°C until use ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids for preparing DNA templates for in vitro transcription were assembled using the pGEM-11Zf(+) (Promega) backbone ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid miniprep DNA isolation was performed using Wizard Plus SV Minipreps DNA Purification System (Promega, USA). Plasmid maxiprep DNA isolation was performed using NucleoBond Extra Maxi Plus EF Kit (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2021Quote: ... These recombinant plasmids were subsequently used to generate digoxigenin-labelled riboprobes using the Riboprobe System (Promega) with SP6 or T3 RNA polymerases and digoxigenin-labelled Uracil triphosphate (Roche ...
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bioRxiv - Microbiology 2020Quote: The plasmids were transfected into the 293T cells using Fugene HD transfection reagent (Promega, Madison, WI). The culture media containing VLP were harvested 3 days post transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor cells were transiently transfected with the plasmid using FuGENE HD transfection reagent (#E2311, Promega, USA) according to manufacturer’s protocol and GFP+ cells were sorted ...
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bioRxiv - Microbiology 2022Quote: ... Positive cells were multiplied and their plasmid extracted using the Wizard Minipreps DNA purification system (Promega). Plasmids were electroporated into WT B ...
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bioRxiv - Neuroscience 2023Quote: ... The linearized plasmids were used as templates for in vitro transcription with T3 RNA polymerase (Promega) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2022Quote: ... Plasmids were purified from these cultures using a Wizard Plus SV Miniprep DNA Purification kit (Promega). All inserted sequences were confirmed by Sanger sequencing using M13 forward and reverse primers (M13F and M13R ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cells were transfected with 1 or 1.5 μg plasmid DNA using FuGene HD (Promega, #E2311) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... pHT315-GFP was then extracted and purified using either the Pureyield plasmid miniprep kit (Promega #A1223) or the QIAGEN® Plasmid Mini Kit (QIAGEN) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5μg of plasmid was transfected into 6-well dishes using Fugene HD (Promega, Madison, WI, USA) with 3:1 transfection reagent to DNA for 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified BAC and plasmid DNA were transfected into HEK293 cells with FuGene HD transfection reagent (Promega) in a 1:3 DNA:FuGene HD ratio ...
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bioRxiv - Biophysics 2023Quote: We stably transfected U2OS cells with this plasmid using Fugene 6 following the manufacturer’s instruction (Promega). α-Amanitin (SIGMA #A2263 ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μg of each purified plasmid was used as input in a 50 μl TnT (Promega) reaction incubated at 25 °C for 2 h ...
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bioRxiv - Physiology 2023Quote: ... cells were transfected with the indicated plasmids using FuGENE® HD Transfection Reagent (#E2311, Promega Corporation) at a ratio of 1:5 (plasmid ...
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bioRxiv - Neuroscience 2023Quote: ... mouseStoml3-GFP10::mCHERRY and mouseElkin1-GFP11::eBP2 plasmids using Fugene HD and following manufacturer’s instructions (Promega). 7 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were prepared by mini or maxi preps using commercially available kits (Qiagen and Promega, respectively) following manufacturers’ instructions ...
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bioRxiv - Immunology 2023Quote: HTR cells were transfected with the indicated plasmids using TransFast transfection reagent (Promega,Madison, WI, USA) according to the manufacturer’s protocol as previously described [9] ...
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bioRxiv - Immunology 2023Quote: ... 1 ng pEF1α-Renilla and 130 ng plasmid of interest and 0.66 µl FuGENE HD (Promega) diluted to 10 µl total volume with OptiMEM ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells were transfected using 2 µg of plasmid DNA and 6 µL of FuGENE (Promega) for gene editing or using a NEPA21 electroporation system (Nepa Gene ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transiently transfected with 1.5 μg of plasmid using FuGENE 6 transfection reagent (#E2692, Promega), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The linearized plasmid was then purified using the Wizard Plus SV Miniprep DNA Purification System (Promega), and saRNA was transcribed in vitro using HiScribe T7 High Yield RNA Synthesis Kit (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02 µg of plasmid and 2 µg of carrier DNA were combined with FuGENE HD (Promega) at a 3:1 ratio and added per well of a 6-well plate seeded with 70% confluent HEK293 cells ...
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bioRxiv - Biophysics 2023Quote: ... cells were transiently transfected using 1g of plasmid and 3ul of FuGENE HD transfection reagent (Promega) based on manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... Other plasmids were obtained from commercial vendors or from the Addgene repository: pRL-CMV-Renilla (Promega), pRS0045 (Addgene #131124) ...
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bioRxiv - Microbiology 2023Quote: ... the pcDNA3.1+ expression plasmids for spike proteins were co-transfected using Fugene (Promega, Madison, WI, USA) into HEK293T/17 cells ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2023Quote: Genomic fragment containing the murine Rorc 3’UTR was cloned into psiCHECK2 luciferase reporter plasmid (Promega). This construct was also used to generate the let-7 ʻseed’ deletion mutant derivative using the QuikChange Multi Site Mutagenesis Kit (catalog 200514-5 ...
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bioRxiv - Cancer Biology 2020Quote: ... T47D cells and MCF7 cells were transfected with empty or human LIP-containing pcDNA3.1 via Fugene HD (Promega) using the manufactures protocol ...
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bioRxiv - Cancer Biology 2021Quote: The upstream 376 bp region of the human LGALS1 transcriptional start site was cloned into the pGL4.23 (Promega) vector to generate the LGALS1 luciferase reporter gene (LGALS1 pGL4.23 ...
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bioRxiv - Cancer Biology 2022Quote: ... and MV4-11 (DSMZ Cat# ACC-102, RRID:CVCL_0064) human AML cell lines were identified by PCR-single-locus-technology (Promega, PowerPlex21 PCR Kit ...
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bioRxiv - Biophysics 2019Quote: The cell-viability of human neuroblastoma (SH-SY5Y) cells was measured using MTT cell proliferation assay (Promega, G4000). SH-SY5Y cells were plated in a 96-well plate followed by differentiation in Neurobasal-A ...