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Citations for Promega :
851 - 900 of 1541 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and T7 RNA polymerase as well as 5 μL RNasin RNase inhibitor (Promega). Transcription reactions were left overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Each reaction contained 5 μL of 2x SYBR Green mastermix (Promega, Benelux BV), 2.5 μL primer mix (forward and reverse ...
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bioRxiv - Biochemistry 2022Quote: ... and impregnated with 75 µL of 5 ng/µL trypsin (trypsin gold; Promega) solution overnight at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... and 175 μg/ml 5-bromo-4-chloro-3- indolyl-phosphate (BCIP) (Promega). Reactions were stopped as the signal became apparent with three PBS rinses ...
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bioRxiv - Immunology 2024Quote: ... 5 mg purified RIPR protein fragment was incubated with TEV protease (Sigma/Promega) at a 1:10 v/v ratio overnight at 4 °C on a rolling mixer ...
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bioRxiv - Cell Biology 2024Quote: ... RNAs were incubated with [5’-32P] pCp and T4 RNA ligase (Promega, M1051) O/N at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 5 µL per well of 20x-NanoBRET Tracer K10 (Promega) at 5 µM in Tracer Dilution Buffer (Promega N291B ...
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bioRxiv - Cancer Biology 2023Quote: ... Livers were incubated using Luciferase Cell Culture Lysis 5 x Reagent (Promega, E1531) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A total of 5 μL per well of 20x-NanoBRET Tracer K10 (Promega) at 10 μM for CSNK2A1 or 5 μM for CSNK2A2 in Tracer Dilution Buffer (Promega N291B ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were directly spiked with 5 pg of unmethylated lambda phage DNA (Promega) and subjected to bisulfite treatment and library construction using the post-bisulfite adaptor tagging (PBAT ...
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bioRxiv - Microbiology 2024Quote: ... were digested with a modified MS grade trypsin (Promega; 1:5 enzyme/protein) overnight at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... containing 5 μL di GoTaq® Green Master Mix (Promega, Madison, WI, USA), 1 μL of each primer 10 μM and 1 μL of template DNA and using a MyCycler Thermal Cycler (BioRAD ...
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bioRxiv - Biochemistry 2024Quote: ... After incubating cells for 10 min with 5 μM coelenterazine-h substrate (Promega), bioluminescence was measured at 535/30 nm and 475/30 nm using a PHERAstar plate reader (BMG) ...
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bioRxiv - Biochemistry 2020Quote: Cell viability was estimated using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, USA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... The sample solutions were diluted to 1 M urea with 50 mM ammonium hydrogen carbonate and trypsin (Promega) was added at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Molecular Biology 2020Quote: Cell proliferation was assessed using either MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega, data are expressed as means of three repeated measures using at least 5 distinct samples for each condition ...
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bioRxiv - Immunology 2021Quote: ... Then the samples were diluted with 340μL of 100mM ABC and 220μL were added to trypsin solution (Promega) for protein digestion at a trypsin/protein ratio of 1/40 and incubated at 37°C overnight (17h) ...
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bioRxiv - Zoology 2021Quote: ... Sections were digested in a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20mg/ml Proteinase K (Promega) ...
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bioRxiv - Microbiology 2021Quote: Cellular viability was determined using non-radioactive CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) reagent (Promega), according to the manufacturer’s recommendations29 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell titer 96® aqueous one solution cell proliferation (MTS) assay (catalog no. G3580) was purchased from Promega Corporation (Madison ...
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bioRxiv - Microbiology 2020Quote: ... cell viability was monitored using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, WI, USA). VeroE6 cells and Huh7.5 cells were seeded in 96-well flat-bottom plates at 10,000 and 9,000 cells per well ...
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bioRxiv - Microbiology 2021Quote: ... 40 μL of the lysate supernatant were mixed with the same volume of Nano-Glo reaction solution (Promega), incubated for 3 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Cells (100 μL) were then incubated with 4 μM cheopin or Fast Break lysis solution (Promega, Madison, WI) and absorbance at 405 nm was measured for 1h using a BioTek Synergy H1M plate reader set to 28°C.
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bioRxiv - Cell Biology 2020Quote: ... and the solution was added to OMEM (75 μL) containing 6 μL Fugene HD reagent (Promega, Madison, WI). The mixture was incubated at room temperature for 10 minutes and gently added to the growth medium on the cultured cells ...
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bioRxiv - Genomics 2020Quote: ... each of the wells containing 0.5 μl of lysis solution (0.2% Triton X-100 [Roche], 0.8 units of RNasin Plus [Promega], 4 mM dNTPs [Promega] and 1 μM of barcoded oligo-dT primers [E3V6NEXT ...
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bioRxiv - Immunology 2021Quote: Cell viability was determined using CellTiter 96® Aqueous One solution according to the manufacturer’s instructions (Promega, Singapore).
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bioRxiv - Cancer Biology 2020Quote: ... Dry gel pieces were digested with 200 μL of mass spectrometry-grade trypsin solution (4 ng/μL, Promega) in AMBIC and incubated at 37°C for 18 h ...
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bioRxiv - Cancer Biology 2022Quote: ... and 100 μl of 50 mM ammonium bicarbonate buffer as well as 10 μl of protease solution (Promega Trypsin/Lys-C Mix ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide solution was then acidified to 0.1% formic acid before adding 0.2 μg Mass Spec grade ProAlanase protease (Promega) and incubating at 37°C for a further 2 h ...
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bioRxiv - Neuroscience 2022Quote: ... cells were pelleted and resuspended in the Homogenization Solution of Maxwell® RSC simplyRNA Tissue Kit (Promega, AS1340) and stored at -80°C until quantitative PCR experiments.
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bioRxiv - Biophysics 2024Quote: ... Ninety microliters of the oocyte supernatant were added to 40 µl of luciferase-luciferin solution (Promega, Madison, WI) for assaying luciferase activity ...
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bioRxiv - Genomics 2022Quote: ... was resuspended in 1.5 mL salt-tris solution buffer supplemented with 0.2 U/ul RNasin Plus RNase Inhibitor (Promega). The tissue material was spin down at 500 x g for 5 min at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were extracted with 1XPLB solution provided in the Dual-Luciferase® Reporter Assay Kit (Promega CN: E1910) and transferred to 96-well black plate to be treated with LARII and Stop & Glo components according to protocol of the Dual-Luciferase® Reporter Assay Kit (Promega CN ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was then isolated from the isopropanol/chloroform solution using the ReliaPrep RNA Cell Miniprep kit (Promega Z6010). For linear RNA depletion ...
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bioRxiv - Cancer Biology 2023Quote: ... 72-hour viability assays were performed using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, G3580) following manufacturer recommendations and 490nM absorbance measured using a CLARIOstar plate reader (BMG Labtech) ...
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bioRxiv - Cell Biology 2023Quote: ... We then mixed the cell solution with 80 μL of NanoGlo luciferase assay reagent (Promega, Cat. No. N1110), transferred it to a 96-well plate well (PerkinElmer,Cat ...
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bioRxiv - Bioengineering 2024Quote: Cell viability was assessed using the CellTiter 96® AQueous One Solution Cell Proliferation (MTS) Assay (Promega, Switzerland). One day before treatment ...
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bioRxiv - Genetics 2024Quote: ... 2 µl of the extracted genome solution was used as a template for Genome PCR by GoTaq (Promega). The sequences of gw182 gene specific primer set designed around the CRISPR target site were 5’ -GAG TCC AAT TTG AGA AAC GGA GGT CA-3’ and 5’ - CTG ATC GTT TGC GCT TAA CTT CAT TAA TTC T-3’ ...
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bioRxiv - Cell Biology 2024Quote: ... which was followed by the addition of 25 μL of a 5X solution of NanoGlo substrate (Promega, #N2161). The cells were then transferred to a white non-binding surface (NBS ...
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bioRxiv - Plant Biology 2024Quote: ... Luminescence was monitored after adding 50 µL of 2X treatment solution via autoinjector using the GloMax Navigator (Promega). After elicitor-induced luminescence was recorded ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was allowed to cool before the addition of 4µl of 4mg/mL RNase A Solution (Promega) before incubation at 37°C for a further hour ...
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bioRxiv - Cancer Biology 2024Quote: ... mitochondrial respiratory chain activity was measured daily using CellTiter 96 AQueous One Solution Reagent (Promega #G3580, Madison, USA) and a microplate reader (iMark ...
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bioRxiv - Molecular Biology 2020Quote: ... Urea-solubilized pellet was then diluted to 2M urea using NaCl/Tris solution (150mM NaCl, 50mM Tris pH 8.0) and digested overnight at 37°C with 10ug of trypsin GOLD (Promega). The following day ...
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bioRxiv - Cell Biology 2020Quote: ... and immersed in 100 µL of growth medium and 20 µL of CellTiter 96 AQueousOne Solution Reagent (Promega Co.). After another incubation for 3 h at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Molecular Biology 2021Quote: ... Gel pieces were rehydrated in 50 mM ammonium bicarbonate solution containing 12.5 ng/µL modified sequencing-grade trypsin (Promega) at 4°C before incubation overnight at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The gel pieces were then first dehydrated with 100 % acetonitrile and then rehydrated with sequence grade trypsin solution (0.6 µg, Promega) and incubated overnight at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: Gently re-suspend cells in 100 μl of 3% Glyoxal fixation solution with 1:25 RNasin Plus (Promega N261B) and incubate for 15 minutes on ice.
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bioRxiv - Molecular Biology 2021Quote: ... 10 μL of stopped reactions were added to 50 µL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a 96-well white microplate ...
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bioRxiv - Genomics 2021Quote: ... The chromatin was then incubated for 30 min at 37° C in 50 µl DNase I solution (1X DNase I buffer containing 0.2 U/µl DNase I [Cat #M6101, Promega] and 0.25 U/µl Protector RNase Inhibitor) ...
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bioRxiv - Microbiology 2020Quote: ... cell viability was assessed by applying 20 µl of CellTiter 96® AQueous One Solution Reagent (Promega, Madison WI) to each well and incubating for 2 h at 25°C ...