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Citations for Promega :
851 - 900 of 4417 citations for Allopregnanolone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... alkylated with 5 mM iodoacetamide for 30 min in the dark and digested overnight with trypsin (Promega; 1 μg for cell-surface-enrichment samples ...
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bioRxiv - Microbiology 2022Quote: ... the modified region was amplified using primer SAM_Seq_Gen-23_FW (5’-GAT TTG AGG ACG ACT GGA CTG C) and SAM_Seq_Gen1116_RV (5’-GTC AAC TGA ACA ACC CCA AGG T) together with GoTaq polymerase (Promega), followed by cloning into pGEM T-easy vector system (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... and after 5 h the luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The embryos were then incubated in a 1:5,000 dilution of TMR HaloTag Ligand (5 mM; Promega) in Danieau’s medium ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by the addition of 5.5μL of a mix containing 5% SDS and 0.25M EDTA and deproteinized with proteinase K (Promega) for 1hour at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... membranes were incubated in a 0.165 mg/ml BCIP (5-bromo-4-chloro-3-indolyl phosphate, Promega)/ 0.33 mg/ml NBT (p-nitroblue tetrazolium chloride ...
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bioRxiv - Molecular Biology 2022Quote: ... the HIV 5’ LTR from the pNL4-3 isolate (Genbank AF324493) was cloned into pGL3-Basic (Promega) via Gibson assembly (NEB 2X HiFi ...
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bioRxiv - Molecular Biology 2019Quote: ... Instrument quality control was monitored using the Promega 6×5 LC-MS/MS Peptide Reference Mix (Promega) before and after each MS experiment run ...
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bioRxiv - Genetics 2020Quote: ... fragments of varying sizes (Supplemental Table 5) were cloned into the promoter-less pGL3-basic vector (Promega) using NheI and NcoI sites ...
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bioRxiv - Biochemistry 2019Quote: ... followed by 5-fold dilution with Milli-Q water and digested with 100 μg of trypsin (Promega) overnight at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL (20x working stock of NanoBRET Tracer K5 [20 μM] in Tracer Dilution Buffer (Promega N291B)) was added to each well with the exception of the “no tracer” control wells ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of RNA were depleted from contaminating DNA by treatment with RQ1 RNase-free DNase (Promega) and were reverse-transcribed with Superscript IV Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Following 5 days of drug incubation cell viability was assayed using 30μl of CellTiter-Glo® (Promega). Screens were performed in technical triplicate.
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bioRxiv - Plant Biology 2022Quote: ... or with the same buffer supplemented with 5 U of calf intestine alkaline phosphatase (CIAP) from Promega. Samples were incubated at 30°C for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... the modified region was amplified using primer SAM_Seq_Gen-23_FW (5’-GAT TTG AGG ACG ACT GGA CTG C) and SAM_Seq_Gen1116_RV (5’-GTC AAC TGA ACA ACC CCA AGG T) together with GoTaq polymerase (Promega), followed by cloning into pGEM T-easy vector system (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were digested via the addition of 5 μL of sequencing grade trypsin (0.1 mg/mL) (Promega) for 3 h at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: A mixture of 10 ng of biotinylated BSA digest and 5 µg of K562 cell digest (Promega) was added to streptavidin beads (10 µL of suspension used ...
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bioRxiv - Physiology 2023Quote: ... Each 10 µl PCR reaction consisted of 5 µl of GoTaq® qPCR Master Mix (Promega Corporation), 0.5 µl of 10 µM forward primer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.5 µg (HEK293A, HEK293T, HEK293AΔARRB1/B2) or 2 µg (HEK293AΔGs, HEKS293AΔGi) Glo-22F cAMP plasmid DNA (Promega) and 2 µg MOCK DNA (HEK293A ...
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bioRxiv - Developmental Biology 2023Quote: ... 16/32-cell embryos were co-injected with 5 pg of pRL-TK:renilla luciferase plasmid (Promega E2241) + 80 pg of the pGL4.23 luc2/miniP HumanPDX1 enhancer:firefly luciferase plasmid (wt or 6RFX motif mutant ...
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bioRxiv - Cancer Biology 2023Quote: ... day 5 and day 7 for each condition using the CellTiter-Glo (CTG) luminescence-based assay (Promega). Diluted CTG reagent (100 uL 1:4 CTG reagent to PBS ...
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bioRxiv - Molecular Biology 2023Quote: The reaction mixture (10 μl) was prepared with 5 μl of rabbit reticulocyte lysate nuclease-treated (Promega), 2 μl of H2O ...
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bioRxiv - Genetics 2024Quote: ... then a 5 min final extension (72 °C)—was carried out with GoTaq Green Master Mix (Promega). Deletion loci from mutant flies were amplified using the genotyping primers above with four GoTaq Green PCR reactions per line ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng of LHB promoter-containing reporter vector were transfected with 5 ng of pRLRenilla (#E2231, Promega), with or without 50 ng of pCMV6-NR2F2-WT or p.Arg246His and/or 5 ng of pCMX-NR5A1 ...
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA was extracted using a DNA extraction kit (Wizard Genomic DNA Purification Kit; Promega Corp, Madison, WI). Genomic DNA was extracted from 1-week old bacterial sub cultures by collecting a loop of bacteria with a sterile pipette tip and inoculating a 0.2 ml PCR tube containing 15 μl of Molecular Grade Water and ran in a thermal cycler for 15 minutes at 95 °C.
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bioRxiv - Plant Biology 2023Quote: ... multiflorum samples using a commercial DNA extraction kit (Wizard HMW DNA Extraction Kit, Promega Corporation, Madison, WI, USA).
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bioRxiv - Cancer Biology 2021Quote: ... were seeded in 96-wells white plate and caspase-1 activities were assessed using Caspase-Glo 1 Inflammasome Assay (Promega #G9951) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Cell growth and viability were measured on the 96 well white bottom plate as an end point measurement for each time point and/or treatment using the Cell-titer GLO assay (Promega, # G7570) based on manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed with PBS and lysed directly on the plate on ice using the Cell Culture Lysis Reagent from the Luciferase Assay System (Promega#E1500). Lysates were transferred to microcentrifuge tubes ...
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bioRxiv - Neuroscience 2021Quote: ... neonatal and adult astrocytes plated on 96-well plates (50,000 cells per well) was measured with the Glucose Uptake-Glo™ Assay (Promega, J1341) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was determined at 72 h post-seeding in 96-well round-bottom ULA cluster plates using CellTiter-Glo® (Promega). Western blot analysis was performed on adherent cell lysates at 72 h post-seeding to confirm DUSP4 knockdown.
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bioRxiv - Molecular Biology 2019Quote: ... 20×103 cells were seeded in a 24-well plate and co-transfected using PEI with pGL3-Chaserr-reporter vector (100 ng) and psiCHECK-1 (100 ng, Promega, C8011). Firefly and Renilla expression were measured using Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Immunology 2021Quote: ... The indicated compounds were introduced analytically using a CyBio pintool and the plates left to incubate at 37 °C prior to the addition of the CellTiter-Glo reagent (Promega, G7573) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: 50 μl supernatant were collected as designed time point into a 96-well plate for lactate dehydrogenase (LDH) release assay as manufacture’s protocol (Promega, cat# G1780). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Crystal violet was the solubilized with a 75% ethanol solution and the absorbance was measured at a λ600nm with a plate reader (Promega GloMax Explorer, Promega, France).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were assayed in white 96 well plates in triplicate by the addition of 50 μl Bright-Glo™ Firefly luciferase assay system (Promega) or freshly prepared 40 ng/ml Coelenterazine in Renilla-Glo buffer (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with the same volume of Nano-Glo® Luciferase reagent (Promega). For the A-MLV titers ...
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bioRxiv - Biochemistry 2021Quote: ... WT or ADAR1-KO HEK293T cells in 12-well plates were transfected with 400 ng ml-1 of firefly luciferase under control of the IFN-β promoter (pIFN-Luc, Promega), 40 ng ml-1 of Renilla luciferase under a constitutive promoter (pRL-TK ...
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bioRxiv - Bioengineering 2021Quote: 100 μL of media was removed from each well on the plate and replaced with an equal amount of CellTiter-Glo reagent (Promega, G7572). Assayed plates were shaken at 500 RPM for 20 minutes and allowed to rest for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Media was refreshed on day 6 and plates collected on day 12 with cell viability quantified by CellTiter-Glo 3D (Promega #G9681). Dose response assay analysis was performed as described above.
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bioRxiv - Genomics 2022Quote: ... F-luciferase and R-luciferase expression was assayed in a TECAN Spark plate reader using the Dual-Glo® Luciferase Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: BD BioCoat Poly-D-Lysine 96-well plates with black sides and clear bottoms were used to run the ApoTox-Glo™ Triplex Assay (Promega). Cells were trypsinized and removed from their culture flasks and diluted to 24,000 cells/ml and 100µl of each sample was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... 48 h later assays were then lysed and read on a FLUOstar Omega plate reader (BMF Labtech) using the Steady-Glo® luciferase assay system (Promega)
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bioRxiv - Molecular Biology 2021Quote: ... and cells with approximately 70-90% confluency in each plate were transfected with required plasmid(s) for each assay using Fugene 6 transfection reagent (Promega, USA) or 1 mg/ml PEI (polyethyleneimine).
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bioRxiv - Microbiology 2020Quote: Plasmids encoding each vector were transfected into HEK293 cells seeded in 10 cm plates using Fugene 6™ (Promega, Madison, WI) or polyethyleneimine ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of each supernatant were transferred into new 96 well plates and equal volume of BacTiter-Glo™ Reagent (Promega) was added per well ...
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bioRxiv - Neuroscience 2019Quote: ... 100 units/mL penicliin and 100 ug/mL streptomycin in 96 well plates (Falcon 353072) and transfected with individual K2Ps using Fugene HD (Promega, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with the same volume of Nano-Glo® Luciferase reagent (Promega). For the VSV-G titers ...
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bioRxiv - Molecular Biology 2021Quote: Cells were plated on a white 96-well plate (Grenier, 655083) and incubated with RealTime-Glo MT Cell Viability Assay reagent (Promega, G9712). The luminescence was detected by GloMax Navigator.
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bioRxiv - Microbiology 2021Quote: ... 30 μl of cell lysis was transferred to a white 96-well assay plate and firefly and renilla activities were measured using the Dual-Glo® Luciferase Assay System (Promega), reading luminescence on a GloMax ®-Multi Detection System (Promega) ...