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Citations for Promega :
801 - 850 of 1564 citations for Recombinant Human CD244 Protein Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... a 3kb region of the promoter containing HIC1 binding sites was amplified by PCR from human genomic DNA and cloned into the pGl4.26-luc vector (Promega); primers are listed in supplementary Table S1 ...
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bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
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bioRxiv - Molecular Biology 2024Quote: ... and the equivalent region in the human (−207/+5) were cloned into pGL3-basic Luciferase reporter-vector (E1751, Promega) using the forward primer containing XhoI restriction site ...
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bioRxiv - Microbiology 2023Quote: ... and Sp-EVs towards human endothelial cells was accessed by the CellTiter-Blue® (CTB) Cell Viability Assay (Promega), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... After 30 minutes of incubation at 37°C in 5% CO2 we added 50,000 ADCC-RL cells (Jurkat cell line expressing luciferase gene under the control of the NFAT response element and stably expressing human FcγRIIIa V158; Promega) in 50 µl RPMI 1640 medium with 6% low-IgG serum (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... TTBK2 KO cell lines were seeded on glass coverslips (Matrigel-coated for hPSCs) and the next day transfected with 0.5μg TTBK1-HaloTag® human ORF in pFN21A (FHC12512, Promega) or pglap1-TTBK2 (“GFP-TTBK2” ...
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bioRxiv - Cancer Biology 2023Quote: IDH1 and NNT promoter sequences were amplified from genomic DNA of human primary melanocytes by PCR and cloned into pGL4.12 (Promega). Mutagenesis of E-boxes was performed using a QuikChange Site-Directed Mutagenesis Kit (Stratagene) ...
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bioRxiv - Neuroscience 2023Quote: ... We amplified the regions from C57Bl/6 tail snip DNA or from human male genomic DNA (Promega catalog # G1471) using FastPhusion 2x Master Mix (Thermo Fisher catalog # F548L) ...
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bioRxiv - Genomics 2023Quote: ... Reference allele enhancer sequences for hZRS and hs737 were obtained via PCR cloning from human genomic DNA (Promega, G304A). Primers used for each enhancer sequence are outlined in Table S2 ...
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bioRxiv - Cell Biology 2024Quote: ... they were transfected with either control plasmid or Flag-GSK-3α WT human plasmid using Fugene 6 (Promega, #E2693) at a 3:1 ratio (DNA:Fugene 6) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
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bioRxiv - Cell Biology 2020Quote: ... Radiolabelled protein was translated from mRNA using the rabbit reticulocyte lysate system (Promega) and 35S-labelled methionine ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was digested for overnight with 5 ng/µL trypsin (Promega Corporation) in 50 mM ammonium bicarbonate at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... The construct expresses rat GR fused with HaloTag protein (Promega, Madison, WI, USA) in the C-terminal domain under the CMVd1 promoter ...
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bioRxiv - Developmental Biology 2020Quote: Proteins were digested on-bead with 0.5 ug Sequencing Grade Modified Trypsin (Promega) overnight at 37°C with agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were digested overnight with sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA).
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bioRxiv - Neuroscience 2021Quote: ... The proteins were digested with 2.5 μg of sequencing grade modified trypsin (Promega). The resulting peptides purified with C18 MicroSpin columns (The Nest Group ...
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bioRxiv - Cell Biology 2019Quote: ... the bead-bound protein was incubated with either 6.7 µM HaloTag-AlexaFluor660 (Promega) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were digested with 2 μg of trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... proteins from HL-1 cells were extracted with Passive Lysis Buffer (Promega France). Quantification of bioluminescence was performed with a luminometer (Centro LB960 ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were digested by adding sequencing grade trypsin (1:20 substrate:enzyme; Promega #5111) and incubating at 37°C for 16 h ...
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bioRxiv - Cell Biology 2020Quote: ... before analysis of luciferase protein levels using Dual-Glo luciferase assay system (Promega) and an EnSpire multimode plate reader (Perkin-Elmer).
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bioRxiv - Systems Biology 2020Quote: The total resultant protein content was digested with trypsin (Trypsin Gold, Promega, USA). The enzyme was added at the ratio of 1:40 (w/w ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were directly digested on the beads by trypsin (Promega, Madison, WI, USA) overnight at 37°C in a 25-mM NH4HCO3 buffer (0.2 µg trypsin in 20 µL) ...
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S-nitrosoglutathione reductase deficiency causes aberrant placental S-nitrosylation and preeclampsiabioRxiv - Physiology 2021Quote: ... 500 μg was digested overnight using 0.02 μg trypsin/μg of protein (Promega). iodoTMT6 labeled samples were combined according to the label’s isotope ...
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bioRxiv - Plant Biology 2021Quote: ... Protein production was confirmed by western blot using an anti-HaloTag antibody (Promega). HaloTag-ligand conjugated magnetic beads (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... One hundred μg of protein from each sample were digested with trypsin (Promega) at 37°C for 24 hours with an enzyme to protein ratio 1:100 (w/w) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested with 0.4 µg of sequencing grade modified trypsin (Promega, #V5111) in 40 µl Hepes/NaOH ...
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bioRxiv - Plant Biology 2021Quote: ... The fusion proteins were purified using the MagneGST™ Pull-Down System (Promega). The subsequent EMSAs were performed using a LightShiftTM Chemiluminescent EMSA Kit (Thermo Fisher ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were then digested to peptides with sequencing grade modified trypsin (Promega V5113), at 37 °C overnight.
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bioRxiv - Microbiology 2019Quote: ... a mixture of the NanoLuc LgBiT protein and luciferase substrate in buffer (Promega) for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were digested by 2 µg sequencing grade trypsin (Promega, Madison, WI) at 37°C with shaking overnight ...
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bioRxiv - Systems Biology 2022Quote: ... Protein enzymatic cleavage was carried out with trypsin (Promega; 1:20, w/w) at 37 °C for 16 h ...
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bioRxiv - Neuroscience 2022Quote: ... proteins were digested overnight at 37°C by the addition of trypsin (Promega) at a 1:100 enzyme:substrate ratio ...
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bioRxiv - Immunology 2022Quote: ... and IgG captured and eluted from Magne Protein A beads (Promega, Madison, WI) as previously described (35 ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested with 0.7 μg Trypsin/Lys-C Mix (MS grade, Promega) in 50 mM ammonium bicarbonate overnight at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... ATP (0.2 mM) and/or protein kinase A (0.1 mg/mL; PKA, Promega) were then added to hydrogel droplets for 12 hours at 25 °C ...
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bioRxiv - Immunology 2021Quote: ... destained and protein digested overnight at 37°C with trypsin (Promega, Madison, Wisconsin). The digested peptide samples were desalted and concentrated using C18 zip tips (Eppendorf ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were finally digested overnight with 200 ng of sequencing grade trypsin (Promega). Samples were analyzed with nanoLC-MS/MS on either a TripleTOF 5600 mass spectrometer (SCIEX ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were digested at room temperature with 0.5 μg Lys-C (Promega, # VA1170) for 4 h ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were in gel digested with trypsin (Trypsin Gold, mass spectrometry grade, Promega) overnight at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were extracted from cultured cells in passive lysis buffer (Promega, cat # E1941) containing protease inhibitors (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins from HL-1 cells were extracted with Passive Lysis Buffer (Promega France). Bioluminescence was quantified with a luminometer (Centro LB960 ...
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bioRxiv - Biophysics 2019Quote: ... Eight-repeats of protein L were inserted into a modified pFN18a vector (Promega), which introduces a HaloTag at the N-terminus and a SpyTag at the C-terminus ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein enzymatic cleavage was carried out with trypsin (Promega; 1:50, w/w) at 37 °C for 16 h ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were subjected to proteolytic digestion overnight by adding sequencing grade trypsin (Promega) at an enzyme:substrate ratio of 1:20 ...
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bioRxiv - Genetics 2020Quote: ... Proteins were digested overnight by adding 1 μg of trypsin (Promega, Madison, Wisconsin) in 100 μL ABC at 37 °C overnight ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were digested overnight by adding 1 μg of trypsin (Promega, Madison, Wisconsin) in 100 μL ABC at 37 °C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was performed using Trypsin Gold (MS grade; Promega, cat. no. V5280). Processed peptides were acidified by formic acid (final concentration 5% ...
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bioRxiv - Plant Biology 2020Quote: ... 100µg protein were digested in solution with RapiGestTM with 1 µg Trypsin (Promega) over night ...