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Citations for Promega :
801 - 850 of 3357 citations for Propane 1 2 Diyl Diacetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 2 ml of Sf9 cells in a 6-well dish at a density of 0.5 x 106 cells/ml were transfected with up to 2 µg of fresh bacmid DNA using FuGene HD transfection reagent (Promega) at a ratio of 3:1 (Fugene reagent:DNA ...
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bioRxiv - Neuroscience 2020Quote: ... the culture medium was replaced with a CO2-independent medium containing 10% (vol/vol) FBS and 2% (vol/vol) GloSensor cGMP stock solution (Promega). The cells were then incubated for 2 h at 27°C in the dark ...
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bioRxiv - Microbiology 2020Quote: ... The 50% tissue culture infectious dose (TCID50) of SARS-CoV-2 pseudovirus was determined using the Steady-Glo luciferase assay system (Promega).
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription was performed using 2 μg of total RNA and oligo(dT)20 primers with M-MLV Reverse Transcriptase (Promega). All results shown include data from three independent experiments ...
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bioRxiv - Microbiology 2020Quote: ... In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously26 ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293T cells and C3H10T1/2 cells were transfected in 6-well plates with various constructs of pmirGlo vector (Promega®). Transfection was performed using Turbofect transfection reagent (Thermo Fischer scientific® ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37 °C with 5% CO2 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected in 96-well plates with 98 ng rTetR plasmid and 2 ng pRL Renilla Luciferase control reporter plasmid (Promega) to control for transfection efficiency ...
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bioRxiv - Immunology 2022Quote: ... In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously ...
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bioRxiv - Molecular Biology 2020Quote: ... RL and FL activities were measured on 96-well plates using 2 µl of cell extract containing 8 µl of 1x PLB (Promega) and the dual-luciferase assay kit (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... and protein complexes were directly digested on-beads by the addition of 2 μg of a Trypsin/Lys-C mixture (Promega). Peptides were isolated on C18 resin tips according to the manufacturer’s instructions (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was purified from 2 × 106 HCT116 cells harvested 48 hr following reporter transfection using the SimplyRNA cells kit and Maxwell RSC instrument (Promega) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... CFPS reactions were diluted 104-106 -fold using 1X PLB+PI buffer (generated by mixing 2 mL of 5x Passive Lysis Buffer E1941 (Promega) with 8 mL water plus one cOmplete™ Mini EDTA-free 11836170001 (Roche ...
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bioRxiv - Systems Biology 2021Quote: ... alkylated in the dark with 2 mM iodoacetamide for 45 minutes at room temperature and digested overnight at 37°C with 80 ng trypsin (Promega). Following digestion ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega (plasmid no. CS1603B118). Construction of the GLP-1R-SmBiT plasmid was described previously (55 ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 VLPs were produced by transfecting 2×106 293T-wt cells in T75 flasks with 25 μg total DNA using Fugene6 (Promega). The codon optimised plasmids expressing SARS-CoV-2 M ...
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bioRxiv - Genetics 2020Quote: ... 2 µg NFIA expression construct (NFIA-FLAG) was transfected to each well of cells by FuGENE HD Transfection Reagent (Promega). Nuclear extracts containing the NFIA protein were extracted from 3 wells of transfected cells using nuclear and cytoplasmic extraction kit (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... An MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium]-based viability assay (CellTiter 96 aqueous nonradioactive cell proliferation assay, Promega) was performed as recommended by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... 50 μL of the supernatant was removed from treated wells and the luminescence values were again recorded on a plate reader (Mithras LB 940, Berthold Technologies) in the presence of the nano luciferase substrate furimazine (2 μL/mL, Promega).
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized from 2 μg total RNA using M-MLV reverse transcriptase with oligo-dT primer (Promega). Expression levels of germination-associated genes were measured by qRT-PCR analysis using LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... and 20 mM iodoacetamide respectively and washed again twice with 200 μl of 100 mM aqueous TEAB before incubating with 2 μg of trypsin (proteomics grade, Promega) at 37°C for 4h ...
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bioRxiv - Biochemistry 2021Quote: Approximately 6 × 107 control cells and 2 × 107 sort2 cells were used for genomic DNA extraction by Wizard Genomic DNA Purification Kit (A1125, Promega). Approximately 325 μg of genomic DNA from control cells and 60 μg of genomic DNA from sort2 cells were used for amplification of gRNA ...
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bioRxiv - Biophysics 2020Quote: ... Plasmids were transfected in cells (passage number < 35) seeded in 96-well plates at ~50 % confluence 2-4 days before the experiment with FuGene6 (Promega) or Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... and bound proteins were digested for ∼14 hr at 37°C in 200 μL of trypsin premix solution containing sequence grade trypsin (2 mg, Promega), urea (0.5 M in 200 mM EPPS pH 8.4) ...
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bioRxiv - Molecular Biology 2021Quote: A total of 10 μg of bacmid DNA was transfected into 6 wells of 2×106 adherent Sf9 cells (at 5×105 cells/ml) using the transfection reagent Fugene HD (Promega). 42–72 h post transfection ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37°C with 5% CO2 ...
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bioRxiv - Genomics 2021Quote: ... Tris(2-carboxyethyl)phosphine (TCEP) and BCA protein assay kit were from Pierce and sequence-grade trypsin was from Promega. Rapigest and Sep-Pak C18 columns were from Waters and C18 Zip tips were from Millipore ...
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bioRxiv - Developmental Biology 2021Quote: ... using primers 1 and 2 (Supplementary Table) and used for making a Digoxigenin (DIG)-labeled FISH probe using T3 RNA polymerase (P2083, Promega). After T3 RNA polymerase reaction at 37°C for 4 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was then digested with 2.5 μg Lys-C (Wako, Japan) for 4 h at 37°C and 2 μg trypsin (Promega, V5113) for an additional 4 h at 37°C ...
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bioRxiv - Bioengineering 2022Quote: HEK293T cells with the RAI13103insC mutation were generated by introducing the plasmid expressing Cas9 and sgRNA with single-stranded oligodeoxynucleotides (Supplementary Sequence 2) by FuGENE HD (Promega). The Cas9-expression plasmid with the sgRNA sequence for generating the RAI13103insC mutation and the GFP/puromycin expression plasmid (70 ng of each plasmid ...
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bioRxiv - Immunology 2022Quote: ... were sorted by FACS in 96-well V-bottom plate wells containing 2 μl of lysis buffer (Ultra-pure Water, 10% Triton X-100, RNasinPlus 40U/ul; Promega). After evaporation of the lysis buffer at 95°C for 3 min ...
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bioRxiv - Plant Biology 2023Quote: ... Purified DNA was run on 2% agarose gel and bands corresponding to ∼150 bp were cut and purified with a Gel Purification kit (Promega). Libraries were constructed using the Nugen Ovation Ultralow Library System V2 following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Protein-protein interactions between Mkl1/2 and Foxo1/3/4 or glucocorticoid receptors were also measured using NanoBiT PPI Starter Systems (Promega). 293FT cells were seeded in 96-well white wall microplates and co-transfected with the LgBiT and SmBiT plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were subjected to in gel tryptic digest overnight at 37 °C with approximately 2 µg trypsin per 100 µl gel volume (Trypsin Gold, mass spectrometry grade, Promega). Peptides were eluted twice with 100 mM NH4HCO3 followed by an additional extraction with 50 mM NH4HCO3 in 50% acetonitrile ...
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bioRxiv - Microbiology 2023Quote: ... gondii glyceraldehyde 3-phosphate dehydrogenase 2 (GAPDH2, ML5049/ML5680) or TgAPT1 (ML4097/ML4098) were used for subsequent PCR amplification with GoTaq polymerase (Promega) for twenty-five cycles as follows ...
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bioRxiv - Microbiology 2023Quote: Vero E6 cells were transfected with an GFP expression vector together with either a control expression vector (no spike) or a SARS-CoV-2 spike expression vector using Fugene (Promega). Two hours after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were diluted to a final concentration of 1e6 cells per mL in Expi293 expression medium plus 2% (v/v) Glosensor assay reagent (Promega) and intubated for 75 min at room temperature with gentle rotation ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were harvested 2 days hours following transfection and reporter assays were performed using the Dual-Luciferase Reporter Assay (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... or PCR amplified from the genome (Tom-3’UTR, primers are in Table S1) and cloned into the psiCHECK-2 vector (Promega) linearized with XhoI and NotI restriction enzymes ...
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bioRxiv - Microbiology 2023Quote: Cells infected with a luciferase-encoding virus were lysed 2 days after infection with a Bright-Glo Luciferase Assay System (E2620, Promega) and the luminescent signal was measured using a GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were washed 3 times by resuspending in cold PBS containing 2% fraction V bovine serum albumin and 0.2 U / µl RNase inhibitor (Promega, N2615). For multiome assay ...
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bioRxiv - Immunology 2023Quote: Expression vectors encoding Foxp3 and Ikzf1 and/or Ikzf1 mutants were transfected into HEK293T cells (2 × 105) with FuGENE HD (Promega). 48h after transfection ...
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bioRxiv - Neuroscience 2022Quote: ... various cDNA fragments spanning the coding region and 3′-UTR were amplified by PCR and cloned individually downstream to the firefly luciferase 2 at the XbaI site of the pmirGLO Dual-Luciferase expression vector (Promega). For the luciferase reporter assay ...
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bioRxiv - Microbiology 2022Quote: ... and a spike-expressing plasmid (pCAGGS-SARS-CoV-2-spike) were co-transfected into HEK293T cells using Fugene HD transfection reagentia (Promega) according to the manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a total of 18 RNA extractions (3 morphs × 3 tissues × 2 biological replicates) were performed using the SV Total RNA Isolation System (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... virus inoculum was removed and cells were then transfected with 2 μg of plasmid pm-cherry-A4L (kindly provided by Wen Chang) using FuGeneHD (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... 48 hours later the media was removed and replaced with 25 µL of RMPI-1640 media supplemented with 2% low-IgG FBS (Promega). Antibodies were serially diluted 1:10 in RPMI 1640 media from a starting concentration of 30 µg/mL to a final concentration of 0.014 µg/mL and 25 µL of antibody dilution was added to the plate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were pre-incubated in Hanks’ balanced salt solution with increasing concentrations of compound for 60 minutes before stimulation with 10 nM CXCL12 and addition of 2 µM Renilla Luciferase substrate coelenterazine-h (Promega). After 20 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 4% FBS and 100 µL were seeded per well in 96-well plates at a density of 2×105 cells/mL in the presence or absence of 0.1 mM HaloTag NanoBRET™ ligand (Promega). The following morning ...
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bioRxiv - Cell Biology 2023Quote: ... Intracellular fluorescence was monitored after 2 and 3 h of nanoalgosome incubation by spectrofluorimetric analysis using a microplate reader (Glomax, Promega). As vehicle-control ...