Labshake search
Citations for Promega :
801 - 850 of 2771 citations for DIRAS Family GTP Binding RAS Like 1 DIRAS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with PBST and then anti-mouse IgG (H&L) alkaline phosphatase-conjugated secondary antibody (Promega) was incubated with the membrane at a 1:5,000 dilution for 1 h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by addition of 25 uL/well of Antibody Dependent Cellular Cytotoxicity (ADCC) Bioreporter Cells (Promega cat# G7010). The ADCC Bioreporter cells are engineered Jurkat cells stably expressing FcγRIIIa V158 (high affinity ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were then digested with 1 μg LysC (Wako) overnight at room temperature and then with 1 μg sequencing grade trypsin (Promega) for 6 hours at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... The reaction was diluted 1:4 with 50 mM ammonium bicarbonate and incubated with 1 µg of Trypsin Gold (Promega) at 37°C for more than 18 h before desalting ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-mouse HRP conjugate (W4021, 1:10,000) and Anti-Rabbit HRP Conjugate (W4011, 1:10,000) were purchased from Promega (Madison, WI). Anti-COPI antibody was a gift from Charles Barlowe (Dartmouth Univ ...
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bioRxiv - Cell Biology 2020Quote: ... HRP Conjugate (W4011, 1:5000), and anti-Mouse IgG (H+L), HRP Conjugate (W4021, 1:5000) were purchased from Promega.
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bioRxiv - Cancer Biology 2021Quote: ... luciferase assay reagent was mixed in a 1:1 ratio with cell lysate (Luciferase Assay System kit, Promega, Madison, WI). Luciferase activity was measured with Synergy H4 Hybrid Reader (BioTek ...
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bioRxiv - Cell Biology 2021Quote: ... Trypsin digestion was carried out overnight at 37°C with 1:50-1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The samples were diluted with 225 μL of 57.6 mM NH4HCO3 containing 1 mM TCEP and the mixtures treated with 1 μg of sequencing grade modified trypsin (Promega), incubated at 37 °C overnight and then acidified by addition of formic acid to 0.1 % v/v and stored at −20 °C ...
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bioRxiv - Cancer Biology 2019Quote: The activity of the inhibitors of hPD-1/hPD-L1 immune checkpoint was examined using the hPD-1/hPD-L1 Blockade Bioassay (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... was dispensed into white 96 well luminometer plates and 45 µL of 1 x NanoGlo Lysis Buffer containing 1:1000 NanoGlo substrate (Promega) was injected into wells ...
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bioRxiv - Cell Biology 2019Quote: ... Cells for gene targeting were transfected with 500 ng of donor and gRNA plasmids in ratio 1:1 using ViaFect (Promega) transfection reagent according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2020Quote: ... the cells for the luciferase assay were exposed to 100 μL of a 1:1 dilution of DMEM and luciferase substrate (ONE-Glo Luciferase Assay System, Promega), and luminescence from each well measured in a GloMax-96 Microplate Luminometer (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were seeded on 24 mm glass coverslips and transfected with 2 µg plasmid DNA (1:1.3:3 ratio LAMP1:ER:opto-kinesin) and Fugene6 transfection reagent (Promega 1:3) for 20-30 h prior to imaging ...
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bioRxiv - Cancer Biology 2019Quote: ... and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE) (hPD-1 Effector Cells, hPD-1 ECs, Promega) were cultured in RPMI-1640 medium (Biowest ...
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bioRxiv - Immunology 2020Quote: ... pCAG-Flag or pCMV-HA-N expression vectors using standard molecular cloning methods as described in our previous publications.30–32 The IFN-β luciferase reporter plasmid pGL3-IFN-β-Luc vector was constructed in our previous study.33,34 The IFNλ1 luciferase reporter plasmid pGL3-IFNλ1-Luc was constructed by inserting the 1000-bp promoter region of human IFNλ1 (nucleotides −1000 to +1, with the translation start site set as 1) into pGL3-Basic (Promega, USA) according to methods outlined in previous studies.13,34 The ISG luciferase reporter plasmid pISRE-Luc vector was purchased from Clontech (USA) ...
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bioRxiv - Molecular Biology 2020Quote: ... and proteins were sequentially digested with LysC (1:50, w/w; FUJIFILM Wako) for 3 h at 37 °C and trypsin (50:1, w/w; Promega) overnight at 37 °C ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Trypsin digestion was carried out overnight at 37 °C with 1:50 - 1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was combined with 69 μL of ammonium bicarbonate along with 1 μL of 1% ProteaseMax surfactant (Promega V2072) for a total volume of 100 μL ...
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bioRxiv - Biophysics 2022Quote: ... Then the samples were diluted to 1 M urea with 50 mM (NH4)HCO3 (AB) and trypsinized (1:50 m/m, Promega) at 37 °C (overnight ...
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bioRxiv - Immunology 2022Quote: ... plates were equilibrated at room temperature for at least 30 minutes before 100 µL of 1:1 diluted PBS and CellTiter Glo reagent (Promega) was added to 100 µL of media ...
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bioRxiv - Molecular Biology 2023Quote: Cell pellets were lysed in cell lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% Triton-X with protease inhibitor cocktail (Promega)) and subjected to Western blotting ...
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bioRxiv - Cancer Biology 2022Quote: ... Urea was added to a final concentration of 1.6 M in 50 mM ammonium bicarbonate and 1 mM calcium chloride prior to digestion with 1 µg sequencing grade porcine trypsin (Promega) for 16 h at 37°C (74) ...
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bioRxiv - Biochemistry 2022Quote: ... Purified AaEnc and BSA were individually buffer exchanged into TBS pH 7.5 and mixed in a 40:1:1 molar ratio with commercially purchased trypsin (Promega, USA) and chymotrypsin (Promega ...
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bioRxiv - Immunology 2023Quote: ... samples were diluted 1:1 with water and digested for 1.5 hours at 37 °C with 1 µg of LysC and overnight at 37 °C with 1 µg trypsin (Promega). The peptide mixture was acidified with trifluoroacetic acid (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... 58 °C for 45 seconds and 72 °C for 1 minute (32 cycles) and 72 °C for 10 minutes (1 cycle) using GoTaq G2 DNA polymerase (Promega). Additionally ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the coding sequence of HIV-1 Nef (SF2 variant) was amplified by PCR and inserted into the plasmid pNLF-1-C (Promega) for expression of NanoLuc at the Nef C-terminus ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl, 5 mM MgCl2, 2 mM DTT, 100 μg ml-1 cycloheximide, and 20 U ml-1 RNase inhibitor [Promega].) Gradients were centrifuged 36,000 rpm for 2.5 h in a SW41 Ti rotor (Beckman Coulter ...
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bioRxiv - Neuroscience 2023Quote: ... urea concentration for each sample was diluted to <1 M using ammonium bicarbonate (ABC) and digested for an additional ∼16 hours using 1:50 (w/w) trypsin (Promega). Following digestion ...
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bioRxiv - Neuroscience 2023Quote: ... UDP (at concentrations ranging from 1 nM to 1 mM) was then applied with a final concentration of 5 μM furimazine (NanoLuc Luciferase Assay, Promega). Luminescence was subsequently measured using a Victor X5 multi-label plate reader (PerkinElmer).
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bioRxiv - Neuroscience 2024Quote: ... the bead and protein mixtures were washed twice with 80% ethyl alcohol and once with 100% ACN before being resuspended in 95 µL NH4HCO3 prior to overnight on-bead digestion (enzyme–protein ratio of 1:20) at 1 000 rpm at 37 °C using modified porcine trypsin/Lys-C Mix (Mass Spectrometry Grade, Promega). The digestion was stopped using trifluoroacetic acid (TFA ...
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bioRxiv - Immunology 2023Quote: The specific activity of Caspase-1 was assessed in cell-free supernatants using Caspase-Glo® 1 Inflammasome Assay (Promega). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were then incubated with blotting buffer containing 1:200 LgBiT for 1-2 h with rocking at RT (N2410, Promega). Next ...
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bioRxiv - Biochemistry 2023Quote: ... pLH3/DTX3L or pLH3/DTX3LΔN) and accessory plasmids pMD2g and psPAX2 (ratio 2:1:1) using ViaFect transfection reagent (Promega E498A). After cell incubation at 37°C for ∼16 h ...
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bioRxiv - Molecular Biology 2023Quote: ... cellular ATP concentrations were measured by adding 20 μL of 1x CellTiterGlo 2.0 (CTG) solution (1 part PBS, 1 part CellTiterGlo2.0 stock solution; Promega Cat# G9241) using a MultidropTM Combi liquid dispenser and measuring the luminescence on a SpectraMax iD3 (Molecular Devices).
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bioRxiv - Biochemistry 2024Quote: ... The binding of the Nbs to the coated protein was detected via their EPEA-tag using a 1:4000 CaptureSelectTM Biotin anti-C-tag conjugate (Thermo Fischer Scientific) in combination with 1:1000 Streptavidin Alkaline Phosphatase (Promega). Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP ...
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bioRxiv - Biochemistry 2023Quote: ... Zc3h18-3F-mAID mES cells were transfected with pB-MYC-mZC3H18x-BSD vectors along with a piggyBAC transposase expressing vector (pBase) in a 1:1 ratio using Viafect (Promega). Cell pools were selected with BSD for ∼ 7-10 days or until negative control cells no longer survived ...
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bioRxiv - Biophysics 2019Quote: ... 1/30 w/w Pro TEV protease (PROMEGA), 1 mM DTT ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-β-galactosidase (Promega, Z378A; 1/100); mouse anti-Myc (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2021Quote: ... β-III TUBULIN (mouse, 1:100 Promega catalogue # G7128), BLBP (rabbit ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 unit of RQ1 RNase-free DNase (Promega) was added to each reaction followed by further incubation for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Either GoTaq 1-Step RT-qPCR System (Promega) was used or reverse transcription for mRNAs was performed using Superscript IV RT (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Rabbit anti-p-JNK (1:50, Promega, V7932), Rat anti-E-cad (1:50 ...
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bioRxiv - Developmental Biology 2021Quote: ... Mouse anti-β-Gal (1:1000, Promega, Z378A). Uptake assay was performed as described previously (Gomez-Lamarca et al ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-β-galactosidase (1:400, Promega Z3781), rabbit anti-Phospho4EBP1 (1:500 ...
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bioRxiv - Biochemistry 2020Quote: ... and digested overnight with 1:50 trypsin (Promega). Peptides were desalted using an MCX protocol (Oasis MCX cartridge 1cc/30 mg LP ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µl RNasin® Ribonuclease Inhibitor (Promega, N2511), 1 µl Maxima H Minus RT (Thermo Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-β-III-tubulin 1:100 (Promega), rabbit anti-neurofilament-L 1:50 (Cell-Signaling Technology) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl 10 mM PCR dNTP mix (Promega) and 0.5 μl RNase free water ...
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bioRxiv - Developmental Biology 2019Quote: ... and 10 U μl–1 RNASin plus (Promega). Transcriptome amplification was performed using 1X KAPA HiFi HS MM and 0.1μM ISPCR primer ...