Labshake search
Citations for Promega :
801 - 850 of 2934 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... samples were diluted 1:1 with water and digested for 1.5 hours at 37 °C with 1 µg of LysC and overnight at 37 °C with 1 µg trypsin (Promega). The peptide mixture was acidified with trifluoroacetic acid (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... 58 °C for 45 seconds and 72 °C for 1 minute (32 cycles) and 72 °C for 10 minutes (1 cycle) using GoTaq G2 DNA polymerase (Promega). Additionally ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the coding sequence of HIV-1 Nef (SF2 variant) was amplified by PCR and inserted into the plasmid pNLF-1-C (Promega) for expression of NanoLuc at the Nef C-terminus ...
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bioRxiv - Cancer Biology 2022Quote: ... Urea was added to a final concentration of 1.6 M in 50 mM ammonium bicarbonate and 1 mM calcium chloride prior to digestion with 1 µg sequencing grade porcine trypsin (Promega) for 16 h at 37°C (74) ...
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bioRxiv - Biochemistry 2024Quote: ... The binding of the Nbs to the coated protein was detected via their EPEA-tag using a 1:4000 CaptureSelectTM Biotin anti-C-tag conjugate (Thermo Fischer Scientific) in combination with 1:1000 Streptavidin Alkaline Phosphatase (Promega). Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP ...
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bioRxiv - Biochemistry 2023Quote: ... Zc3h18-3F-mAID mES cells were transfected with pB-MYC-mZC3H18x-BSD vectors along with a piggyBAC transposase expressing vector (pBase) in a 1:1 ratio using Viafect (Promega). Cell pools were selected with BSD for ∼ 7-10 days or until negative control cells no longer survived ...
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bioRxiv - Neuroscience 2024Quote: ... the bead and protein mixtures were washed twice with 80% ethyl alcohol and once with 100% ACN before being resuspended in 95 µL NH4HCO3 prior to overnight on-bead digestion (enzyme–protein ratio of 1:20) at 1 000 rpm at 37 °C using modified porcine trypsin/Lys-C Mix (Mass Spectrometry Grade, Promega). The digestion was stopped using trifluoroacetic acid (TFA ...
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bioRxiv - Immunology 2023Quote: The specific activity of Caspase-1 was assessed in cell-free supernatants using Caspase-Glo® 1 Inflammasome Assay (Promega). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were then incubated with blotting buffer containing 1:200 LgBiT for 1-2 h with rocking at RT (N2410, Promega). Next ...
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bioRxiv - Biochemistry 2023Quote: ... pLH3/DTX3L or pLH3/DTX3LΔN) and accessory plasmids pMD2g and psPAX2 (ratio 2:1:1) using ViaFect transfection reagent (Promega E498A). After cell incubation at 37°C for ∼16 h ...
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bioRxiv - Molecular Biology 2023Quote: ... cellular ATP concentrations were measured by adding 20 μL of 1x CellTiterGlo 2.0 (CTG) solution (1 part PBS, 1 part CellTiterGlo2.0 stock solution; Promega Cat# G9241) using a MultidropTM Combi liquid dispenser and measuring the luminescence on a SpectraMax iD3 (Molecular Devices).
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bioRxiv - Biochemistry 2022Quote: ... The sample was combined with 69 μL of ammonium bicarbonate along with 1 μL of 1% ProteaseMax surfactant (Promega V2072) for a total volume of 100 μL ...
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bioRxiv - Biophysics 2022Quote: ... Then the samples were diluted to 1 M urea with 50 mM (NH4)HCO3 (AB) and trypsinized (1:50 m/m, Promega) at 37 °C (overnight ...
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bioRxiv - Immunology 2022Quote: ... plates were equilibrated at room temperature for at least 30 minutes before 100 µL of 1:1 diluted PBS and CellTiter Glo reagent (Promega) was added to 100 µL of media ...
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bioRxiv - Molecular Biology 2023Quote: Cell pellets were lysed in cell lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% Triton-X with protease inhibitor cocktail (Promega)) and subjected to Western blotting ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl, 5 mM MgCl2, 2 mM DTT, 100 μg ml-1 cycloheximide, and 20 U ml-1 RNase inhibitor [Promega].) Gradients were centrifuged 36,000 rpm for 2.5 h in a SW41 Ti rotor (Beckman Coulter ...
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bioRxiv - Neuroscience 2023Quote: ... urea concentration for each sample was diluted to <1 M using ammonium bicarbonate (ABC) and digested for an additional ∼16 hours using 1:50 (w/w) trypsin (Promega). Following digestion ...
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bioRxiv - Neuroscience 2023Quote: ... UDP (at concentrations ranging from 1 nM to 1 mM) was then applied with a final concentration of 5 μM furimazine (NanoLuc Luciferase Assay, Promega). Luminescence was subsequently measured using a Victor X5 multi-label plate reader (PerkinElmer).
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were visualized on 1% agarose gels (90 V, 30-45 minutes) against a 1 kb DNA ladder (Promega). Sanger sequencing was performed by the sequencing unit at the Max Planck Institute for Evolutionary Biology ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 hr incubation in a 1:10000 dilution of the secondary antibody (anti-mouse IgG HRP conjugate, W402B, Promega) at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 hr incubation in a 1:10000 dilution of the secondary antibody (anti-mouse IgG HRP conjugate, W402B, Promega) at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 50μL of clarified lysates were transferred to a 96-well plate (white, flat-bottom, SPL) and mixed (1:1) with BacTiter-Glo (Promega) reaction buffer followed by incubation at 37°C for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... into 96-well PCR plates with 1 μL of cell lysis solution (1:10 Cell Lysis buffer [Roche], 10 U/μL Rnasin plus Ribonuclease inhibitor [Promega]) in each well ...
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bioRxiv - Biochemistry 2024Quote: ... Purified Haspin (465-498; 250 nM) was incubated for 1 hour at room temperature following addition of 1 mM ATP (Promega) in a reaction volume of 50 µL (in 50 mM Tris-Cl pH 7.6 ...
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bioRxiv - Molecular Biology 2024Quote: ... Wildtype mES cells were transfected with pB[MYC-] or pB[MYC-mTurbo-PHAX] vectors along with a piggyBAC transposase expressing vector (pBase) in a 1:1 ratio using Viafect (Promega). Cell pools were selected for successful integration of pB plasmids with Blastocidin (BSD ...
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bioRxiv - Plant Biology 2024Quote: ... columns were pre-equilibrated with 5 CV of 0.1 M NH4CO3 and then loaded with 1 CV of 0.1 M NH4CO3 containing 1 µg/ml trypsin gold (Promega V5280, Promega, USA). The flow-through containing the digested peptides of S-nitrosated proteins (elution 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-β-galactosidase (Promega, Z378A; 1/100); mouse anti-Myc (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2021Quote: ... β-III TUBULIN (mouse, 1:100 Promega catalogue # G7128), BLBP (rabbit ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 unit of RQ1 RNase-free DNase (Promega) was added to each reaction followed by further incubation for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Either GoTaq 1-Step RT-qPCR System (Promega) was used or reverse transcription for mRNAs was performed using Superscript IV RT (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Rabbit anti-p-JNK (1:50, Promega, V7932), Rat anti-E-cad (1:50 ...
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bioRxiv - Developmental Biology 2021Quote: ... Mouse anti-β-Gal (1:1000, Promega, Z378A). Uptake assay was performed as described previously (Gomez-Lamarca et al ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-β-galactosidase (1:400, Promega Z3781), rabbit anti-Phospho4EBP1 (1:500 ...
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bioRxiv - Biochemistry 2020Quote: ... and digested overnight with 1:50 trypsin (Promega). Peptides were desalted using an MCX protocol (Oasis MCX cartridge 1cc/30 mg LP ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µl RNasin® Ribonuclease Inhibitor (Promega, N2511), 1 µl Maxima H Minus RT (Thermo Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl 10 mM PCR dNTP mix (Promega) and 0.5 μl RNase free water ...
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bioRxiv - Biochemistry 2020Quote: ... 100 U ml−1 RNasin RNase inhibitor (Promega)) ...
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bioRxiv - Biochemistry 2020Quote: ... Sequencing grade modified trypsin (1 μg; Promega; V5113) was added to the sample before incubation overnight at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and Halotag (Promega, Cat#: G9281, 1:200 dilution) antibodies were used in blocking solution ...
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bioRxiv - Cancer Biology 2020Quote: ... and digested with 1 µg trypsin (Promega, V5113) over night at 37°C while shaking ...
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bioRxiv - Cancer Biology 2020Quote: ... and 0.2 mg ml-1 Proteinase K (Promega). Samples were analysed by qPCR with primers specific for the promoters of SLC7A11 and CDKN1A ...
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bioRxiv - Cell Biology 2021Quote: ... 20 U SP6 RNA polymerase (Promega; 1 µl). Following a 1-h incubation of the cocktail at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM PMSF and protease inhibitor mix (Promega) was used to resuspend frozen pellet that was thawed on ice ...
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bioRxiv - Biophysics 2020Quote: ... 1/30 w/w Pro TEV protease (PROMEGA), 1 mM DTT ...
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bioRxiv - Microbiology 2022Quote: ... 0.5-1 unit of RNAse-inhibitor (RNAsin, Promega), and 0.5-1 ul of enzyme mix ...
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bioRxiv - Cell Biology 2022Quote: ... 1:2000 HRP-conjugated anti-mouse IgG (Promega) secondary antibody was applied for two hours at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... lysed in 1× Passive Lysis buffer (#E1910, Promega), and incubated at RT for 15 min on a rotor disk ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL RNasin® Ribonuclease Inhibitor (Promega, N2511), 1-2 µL TGIRT (InGex ...
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bioRxiv - Cell Biology 2022Quote: ... anti-mouse IgG HRP (Promega, WB 1:10000) and anti-rabbit IgG HRP (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL RNasin® Ribonuclease Inhibitor (Promega, N2511), 1 µL TGIRT and nuclease-free water up to 19 µL ...