Labshake search
Citations for Promega :
751 - 800 of 1637 citations for Tetratricopeptide repeat protein 14 TTC14 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... Protein digestion was accomplished by overnight incubation at 37 °C with trypsin (V5111, Promega) at a 1:20 enzyme to protein mass ratio ...
-
bioRxiv - Biophysics 2022Quote: ... The protein was digested at 37 °C overnight by adding sequencing-grade trypsin (Promega) at a 24:1 substrate-to-enzyme ratio ...
-
bioRxiv - Biochemistry 2022Quote: ... ⑤Resuspended the denatured proteins with 25 mmol/L NH4HCO3 and digested with trypsin (Promega, Fitchburg ...
-
bioRxiv - Plant Biology 2022Quote: ... PsiYAB11 protein was expressed using the TNT SP6 Coupled Wheat Germ Extract System (Promega). The protein-bound beads were incubated with 50 ng of adaptor-ligated gDNA fragments on a rotator for 1 h at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: In-gel protein digestion of every spot was performed using sequencing-grade trypsin (Promega) as described elsewhere (26) ...
-
bioRxiv - Neuroscience 2023Quote: ... proteins were digested overnight at 37°C using MS-grade trypsin (Promega, Cat# V5280). The next morning ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein enzymatic cleavage was carried out with trypsin (1:20, w/w; Promega, V5280) at 37 °C for 16 h ...
-
bioRxiv - Molecular Biology 2022Quote: Fifty μl protein expression reaction using TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600) containing 1000 ng pIX-Halo-bZIP plasmids was incubated for 3 h at 30°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... proteins were precipitated with acetone and trypsin-digested using sequencing grade-modified trypsin (Promega). The resulting peptide mixture was purified by STAGE-TIP method using a C18 resin disk (3M Empore ...
-
bioRxiv - Plant Biology 2022Quote: ... Proteins eluted at pH 9.0 were further treated with 5 U of CIAP (Promega) and the buffer provided by the manufacturer (a negative control was prepared without phosphatase) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The proteins were digested with sequencing-grade modified trypsin (Promega Corporation, Madison, WI, USA). The peptides were analyzed using a nano LC–MS system (UltiMateTM 3000 RSLCnano and Orbitrap ExplorisTM 240 ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were digested for 18 h with trypsin/Lys-C Mix (Promega Corporation, USA) and 10 μg of each sample was used for proteome analysis ...
-
bioRxiv - Molecular Biology 2022Quote: ... proteins were digested with chymotrypsin (5 ng/μL) at 25ºC overnight (Trypsin gold, Promega). Digestion was stopped by addition of 5% formic acid and peptides extracted twice with 70% acetonitrile and 5% formic acid (10 min sonication) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and the proteins were digested with 1/100 (w/w) trypsin (Promega, Cat # V5111) overnight at 37°C ...
-
bioRxiv - Microbiology 2024Quote: ... Prior to tryptic digestion of the protein overnight using mass-spec grade Trypsin (Promega), protein was reduced and alkylated using dithiothreitol and iodoacetamide ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins were digested using filter-aided sample preparation with sequence-grade trypsin (Promega, # V5111) at 37°C overnight ...
-
bioRxiv - Immunology 2024Quote: ... with a 1:50 ratio of purified protein to Endoglycosidase H (Endo H) (Promega). After treatment ...
-
bioRxiv - Bioengineering 2024Quote: ... The reactions were performed using the S30 T7 High-Yield Protein Expression System (Promega). 3 μl of peptide solution were added to reaction mixtures to get a final concentration respectively of 32 μg/ml or 512 μg/ml in a final volume of 15 μL in nuclease-free PCR tubes ...
-
bioRxiv - Molecular Biology 2024Quote: ... 50 ng/μl of mRNA and 6 ng/μl QuantiLum Recombinant Luciferase protein (Promega) were coinjected ...
-
bioRxiv - Biochemistry 2024Quote: ... the blotted proteins were developed using Nano-Glo® In-Gel Detection System (Promega) and scanned with a ChemiDoc MP Imaging System (Bio-Rad).
-
bioRxiv - Biochemistry 2024Quote: ... The HaloTag-JF503-SWSAP1 was quantified by using recombinant HALO-GFP protein standard (Promega) pre-incubated with JF503 at a 1:1 molar ratio and titrated into single molecule buffer (20 mM Tris pH7.5 ...
-
bioRxiv - Systems Biology 2023Quote: ... dark) and digestion using 0.015 µg Trypsin for 1.5 µg protein (Trypsin Gold, Promega, Fitchburg ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were digested by trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega; protein:enzyme (w/w) ratio – 100:1 ...
-
bioRxiv - Cell Biology 2023Quote: ... The proteins were then digested into peptides with sequencing grade modified trypsin (Promega V5113) at 37 °C overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
-
bioRxiv - Biochemistry 2023Quote: Eluted proteins were digested with Lys-C (FUJIFILM Wako) and trypsin (Promega, Madison, WI) according to the PTS protocol21 ...
-
bioRxiv - Biophysics 2023Quote: ... the desired volume of protein solution was incubated with Factor Xa (Promega, Madison, WI). All experiments were carried out in a tris buffer (150 mM NaCl ...
-
bioRxiv - Cell Biology 2023Quote: ... and proteins were further digested by the addition of 2 μg trypsin (Promega #V5113) for 4 hours at room temperature on a shaker ...
-
bioRxiv - Physiology 2024Quote: ... Chemiluminescent detection of in vitro translated protein was performed following the manufacturer’s protocol (Promega).
-
Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested overnight at 37 °C with LysC (Wako Chemicals) and trypsin (Promega) in a 1/100 enzyme-to-substrate ratio ...
-
bioRxiv - Microbiology 2024Quote: The CHIKV ORF encoding for viral structural proteins was cloned into pCI-Neo (Promega) to obtain pCI-neo-CHIKV ...
-
bioRxiv - Biochemistry 2024Quote: ... proteins were digested in-gel using trypsin (500 ng/band, Gold, Promega, Ref V5280). Digest products were dehydrated in a vacuum centrifuge and reduced to 3 μL ...
-
bioRxiv - Molecular Biology 2024Quote: ... 3 volumes of TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) was incubated with 2 volumes of 100 ng/μL purified plasmid at 25°C for 2 hours and used immediately.
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were then digested overnight at 37°C with 3 μg Trypsin/LysC (Promega). The digest was then acidified with formic acid and desalted (C18 HyperSep columns) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 50 µg of total protein were incubated with 1 µg of porcine trypsin (Promega) in presence of 0.5 % SLS at 30 °C overnight ...
-
bioRxiv - Biochemistry 2024Quote: ... Endogenous protein levels were quantified using the Nano-Glo HiBiT Lytic Detection System (Promega) as described in manufacturer’s manual ...
-
bioRxiv - Biochemistry 2024Quote: Limited proteolysis experiments for WT FL-HsFN3K protein were done using either Thermolysin (Promega) or Chymotrypsin (Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... and protein enzymatic cleavage was carried out with trypsin (Promega; 1:20, w/w) at 37 °C for 16 h as previously described (Shevchenko et al ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The proteins were then digested in situ with sequencing-grade trypsin (Promega, Madison, WI), following the method described by Pérez et al.106 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). Membranes were developed and visualized using the Pierce™ ECL immunoblotting substrate (ThermoFisher Scientific).
-
bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
-
bioRxiv - Microbiology 2024Quote: ... Correct protein size of 41.4 kDa for recEmTDH and 40.3 kDa for recMmTDH was confirmed by western blot using a mouse monoclonal anti-His tag antibody and an anti-mouse IgG (h+l) ap conjugate as secondary antibody (Promega, Dübendorf, Switzerland). Finally ...
-
bioRxiv - Developmental Biology 2022Quote: ... Anti–β-galactosidase monoclonal mouse antibody (Promega, 1:500) was co-incubated with anti-mCherry polyclonal rabbit antibody (Bio Vision ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody (Promega) (1:10,000 ...
-
bioRxiv - Cell Biology 2020Quote: ... Rabbit anti-HaloTag antibody (G9218) was purchased from Promega, and Donkey anti-rabbit IgG (minimal cross reaction to Bovine ...
-
bioRxiv - Plant Biology 2021Quote: ... Anti-rabbit IgG horseradish peroxidase-linked antibody (Promega, USA) was used as the secondary antibody at 1:10,000–20,000 dilution ...
-
bioRxiv - Neuroscience 2021Quote: ... The immunoblots were probed with appropriate secondary antibodies (Promega) and visualised by chemiluminescence (Supersignal detection kit ...
-
Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... Secondary antibody (donkey α-goat HRP, Promega, 1:10,000). For His-tagged proteins ...
-
bioRxiv - Immunology 2021Quote: ... incubated with anti-IgY secondary antibody (Promega Madison, USA) for an hour ...
-
bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies (Anti-Rabbit/Mouse IgG HRP Conjugate, Promega) were used at 1:5000 in 5% Blotting-Grade Blocker (BioRad).