Labshake search
Citations for Promega :
751 - 800 of 1305 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... proteins were generated with pCS2-N-terminal 5×MYC vectors and pCS2-N-terminal 3×HA vectors described above using TnT® Coupled Reticulocyte Lysate System under the SP6 promoter (L4600, Promega) and by following manufacturer’s recommendations with few modifications ...
-
bioRxiv - Molecular Biology 2023Quote: 10 μL in vitro translation reactions were performed in the linear range using 3 nM mRNA in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 30% RRL ...
-
bioRxiv - Genomics 2023Quote: ... The lysates were generated by adding 100 µL 1X Passive Lysis Buffer included in the Dual-luciferases assay kit (Promega E1960) to the protoplasts and vigorously shaking at room temperature for 15 minutes ...
-
bioRxiv - Neuroscience 2024Quote: ... One µL of lysate was then added to make 20µL PCR reactions with GoTaq G2 Green Master Mix (Promega, Cat# M7823) and 1µM forward and reverse primers (Table 1) ...
-
bioRxiv - Cell Biology 2020Quote: ... SCC1-Halo was purified and fluorescently labeled with Janelia Fluor 549 HaloTag (Promega) as previously described (5).
-
bioRxiv - Genetics 2021Quote: ... purified using the Wizard® SV Gel and PCR Clean-Up System (Promega), cloned into the pGEM-T Easy vector (Promega) ...
-
Relationship between True Digestibility of dietary Phosphorus and Gastrointestinal Bacteria of GoatsbioRxiv - Microbiology 2019Quote: ... The amplicons were purified with a PCR Clean-Up system (Promega, Madison, USA) by a purification kit (QIAGEN ...
-
bioRxiv - Molecular Biology 2021Quote: ... and purified twice with a miniprep kit (PureYield™ Plasmid Miniprep System, Promega). Plasmids were eluted with RNase free water and used as templates for in vitro gene expression (E ...
-
bioRxiv - Molecular Biology 2020Quote: ... purified using the Wizard® SV Gel and PCR Clean-Up System (Promega) or MinElute® PCR Purification Kit (Qiagen ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... approximately 5.2 µg of purified gDNA (spiked with 1% unmethylated lambda DNA, Promega) was sheared into fragment size of 200-300 bp using Covaris S220 ...
-
bioRxiv - Molecular Biology 2019Quote: ... DNA samples were then purified using the Wizard SV PCR purification kit (Promega) according to the manufacturer’s protocol.
-
bioRxiv - Molecular Biology 2021Quote: ... The fragments excised from the gel purified by the DNA Purification kit (Promega) according to the maker instructions.
-
bioRxiv - Plant Biology 2020Quote: ... Purified products were cloned into the pGEM®-T Easy Vector Systems (Promega) for Sanger sequencing.
-
bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Wizard PCR Preps DNA Purification System (Promega) and DNA sequencing was performed using primer 2569 ...
-
bioRxiv - Plant Biology 2021Quote: ... The fusion proteins were purified using the MagneGST™ Pull-Down System (Promega). The subsequent EMSAs were performed using a LightShiftTM Chemiluminescent EMSA Kit (Thermo Fisher ...
-
bioRxiv - Neuroscience 2022Quote: ... Purified and digested DNA fragment was cloned into a pGL3-Enhancer vector (Promega) and the correct sequence was checked by Sanger sequencing ...
-
bioRxiv - Developmental Biology 2022Quote: ... and purified using a Wizard SV Gel and PCR Clean-up System (Promega). mRNA was generated from linearized plasmid using an SP6 mMessage mMachine Transcription Kit (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: Plasmid DNA was isolated and purified using a PureYield Plasmid Miniprep System (Promega) and gel electrophoresis were carried out using standard techniques (Sambrook and Russell ...
-
bioRxiv - Genomics 2019Quote: ... The DNA was then purified and ligated with a T4 DNA ligase (Promega). Next ...
-
bioRxiv - Cell Biology 2019Quote: ... The PCR products were purified using the Wizard DNA Clean-up System (Promega), and then cloned into the pGEM-T Easy Vector I (Promega) ...
-
bioRxiv - Cancer Biology 2019Quote: ... cDNA was yielded from purified mRNA using ImProm II Reverse Transcription kit (Promega). Quantitative-RT PCR was then performed using the SensiFAST SYBR Green Hi-ROX master mix (Bioline) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Both PCR products were purified using the Wizard PCR cleanup kit (Promega, USA).
-
bioRxiv - Microbiology 2020Quote: ... pKAS32 was digested with XbaI and SacI and purified by gel extraction (Promega). Primers (Integrated DNA Technologies ...
-
bioRxiv - Zoology 2021Quote: ... PCR products were purified with Wizard SV Gel and PCR Cleanup kit (Promega). Purified products were sequenced at Sequetech DNA Sequence Service (Mountain View ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... The purified PCR products were ligated with the pGEM®-T vector (Promega) as recommended by the manufacturer and subsequently sequenced (Macrogen) ...
-
bioRxiv - Genomics 2021Quote: ... The PCR products were purified via gel excision and column purification (Promega, A9282), and then inserted into the BsmBI-digested lentiCRISPR-v2-Blast-Puro vector by Gibson assembly (New England Biolabs ...
-
bioRxiv - Biophysics 2020Quote: eGFP was purified and labeled with HaloTag Succinimidyl Ester (O4) Ligand (Promega, P6751) as previously described (Huang ...
-
bioRxiv - Cell Biology 2020Quote: ... the plasmids with subcloned genes were purified with PureYield Plasmid Miniprep System (Promega) and transfected into adherent cell lines using jetPRIME transfection reagent (Polyplus Transfection).
-
bioRxiv - Microbiology 2020Quote: ... The PCR product was purified by gel extraction and digested with DpnI (Promega) for 1 h to remove residual template DNA before a second round of purification ...
-
bioRxiv - Immunology 2020Quote: ... the water phase was further purified using the ReliaPrepTM RNA Miniprep system (Promega) per manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... DNA was purified using the Pure Yield Plasmid Midi Prep System (Promega, A2495).
-
bioRxiv - Bioengineering 2022Quote: ... PCR product was purified and ligated to the pGEM®-T vector (Promega). The construction was transformed into NEB® Turbo competent E ...
-
bioRxiv - Molecular Biology 2022Quote: ... The DNA was then purified and ligated with a T4 DNA ligase (Promega). Next ...
-
bioRxiv - Genomics 2022Quote: ... Purified DNA was prepared for quantification using the Qubit HS-dsDNA System (Promega) quantified using a Qubit Fluorometer (Thermo Fisher ...
-
bioRxiv - Immunology 2022Quote: ... A-tailing of purified PCR products was performed using GoTaq polymerase (Promega, UK) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2022Quote: ... The resultant linear transgene was purified (Wizard® Genomic DNA purification kit, Promega) and confirmed by sequencing (oligos p001-p006 ...
-
bioRxiv - Genomics 2022Quote: ... diluted to 1% in purified human genomic DNA (Promega, female, catalog No. G1521). Briefly ...
-
bioRxiv - Molecular Biology 2022Quote: Purified EXT1-EXT2 was digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described67 ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA was then purified using a Promega PCR Clean-Up Kit (Promega #A9282), quantified (Synergy HTX ...
-
bioRxiv - Molecular Biology 2022Quote: ... purified using the PureYield Plasmid Miniprep or Midiprep Systems (Promega # A1222 and A2495), and validated by Sanger sequencing at The Ohio State University Comprehensive Cancer Center Genomics Shared Resource (OSUCCC GSR) ...
-
bioRxiv - Immunology 2022Quote: ... and purified with the Wizard SV Gel and PCR Clean-Up System (Promega) after DNA electrophoresis ...
-
bioRxiv - Immunology 2023Quote: ... Total RNA was purified using a ReliaPrep RNA Tissue Miniprep kit (Promega, z6112). The RNA was sent to Kazusa Genome Technologies to perform the library preparation and sequencing ...
-
bioRxiv - Microbiology 2023Quote: ... RNA was further purified and DNA digested on a Maxwell RSC instrument (Promega) with the Maxwell RSC simplyRNA tissue kit ...
-
bioRxiv - Bioengineering 2023Quote: ... The amplicon was purified Wizard SV Gel and PCR Clean-up System (Promega) and was used to produce preselected libraries ...
-
bioRxiv - Biochemistry 2023Quote: ... purified using the PureYield Plasmid Miniprep or Midiprep Systems (Promega # A1222 and A2495), and validated by Sanger sequencing at The Ohio State University Comprehensive Cancer Center Genomics Shared Resource (OSUCCC GSR) ...
-
bioRxiv - Systems Biology 2023Quote: ... Halo-fusion proteins were purified with Magne® HaloTag® Beads (Promega, USA) and then incubated with 50 ng ampDAP library ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Purified RNA was reverse-transcribed into cDNAs with GoScript Reverse Transcription System (Promega) according to the manufacturer’s instruction ...
-
bioRxiv - Biochemistry 2023Quote: ... DNA used for transfection was purified using the PureYield Plasmid Midiprep System (Promega). After incubation for 30 min ...
-
bioRxiv - Biochemistry 2023Quote: ... and digestion of purified crosslinked protein with sequencing grade modified porcine trypsin (Promega), tryptic peptides were separated by reverse phase XSelect CSH C18 2.5 um resin (Waters ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA was purified using the Maxwell RSC simply RNA tissue extraction kit (Promega) with on-bead DNase I digestion ...