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Citations for Promega :
751 - 800 of 1615 citations for F actin uncapping protein LRRC16A CARMIL1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The protein was digested at 37 °C overnight by adding sequencing-grade trypsin (Promega) at a 24:1 substrate-to-enzyme ratio ...
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bioRxiv - Biochemistry 2022Quote: ... ⑤Resuspended the denatured proteins with 25 mmol/L NH4HCO3 and digested with trypsin (Promega, Fitchburg ...
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bioRxiv - Plant Biology 2022Quote: ... PsiYAB11 protein was expressed using the TNT SP6 Coupled Wheat Germ Extract System (Promega). The protein-bound beads were incubated with 50 ng of adaptor-ligated gDNA fragments on a rotator for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: In-gel protein digestion of every spot was performed using sequencing-grade trypsin (Promega) as described elsewhere (26) ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were digested overnight at 37°C using MS-grade trypsin (Promega, Cat# V5280). The next morning ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
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bioRxiv - Biochemistry 2023Quote: Eluted proteins were digested with Lys-C (FUJIFILM Wako) and trypsin (Promega, Madison, WI) according to the PTS protocol21 ...
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bioRxiv - Biophysics 2023Quote: ... the desired volume of protein solution was incubated with Factor Xa (Promega, Madison, WI). All experiments were carried out in a tris buffer (150 mM NaCl ...
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bioRxiv - Physiology 2024Quote: ... Chemiluminescent detection of in vitro translated protein was performed following the manufacturer’s protocol (Promega).
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested overnight at 37 °C with LysC (Wako Chemicals) and trypsin (Promega) in a 1/100 enzyme-to-substrate ratio ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were digested in-gel using trypsin (500 ng/band, Gold, Promega, Ref V5280). Digest products were dehydrated in a vacuum centrifuge and reduced to 3 μL ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 volumes of TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) was incubated with 2 volumes of 100 ng/μL purified plasmid at 25°C for 2 hours and used immediately.
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bioRxiv - Microbiology 2024Quote: The CHIKV ORF encoding for viral structural proteins was cloned into pCI-Neo (Promega) to obtain pCI-neo-CHIKV ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were then digested overnight at 37°C with 3 μg Trypsin/LysC (Promega). The digest was then acidified with formic acid and desalted (C18 HyperSep columns) ...
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bioRxiv - Biochemistry 2024Quote: ... Endogenous protein levels were quantified using the Nano-Glo HiBiT Lytic Detection System (Promega) as described in manufacturer’s manual ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50 µg of total protein were incubated with 1 µg of porcine trypsin (Promega) in presence of 0.5 % SLS at 30 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: Limited proteolysis experiments for WT FL-HsFN3K protein were done using either Thermolysin (Promega) or Chymotrypsin (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... and protein enzymatic cleavage was carried out with trypsin (Promega; 1:20, w/w) at 37 °C for 16 h as previously described (Shevchenko et al ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The proteins were then digested in situ with sequencing-grade trypsin (Promega, Madison, WI), following the method described by Pérez et al.106 ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). Membranes were developed and visualized using the Pierce™ ECL immunoblotting substrate (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
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bioRxiv - Microbiology 2024Quote: ... Correct protein size of 41.4 kDa for recEmTDH and 40.3 kDa for recMmTDH was confirmed by western blot using a mouse monoclonal anti-His tag antibody and an anti-mouse IgG (h+l) ap conjugate as secondary antibody (Promega, Dübendorf, Switzerland). Finally ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti–β-galactosidase monoclonal mouse antibody (Promega, 1:500) was co-incubated with anti-mCherry polyclonal rabbit antibody (Bio Vision ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody (Promega) (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit anti-HaloTag antibody (G9218) was purchased from Promega, and Donkey anti-rabbit IgG (minimal cross reaction to Bovine ...
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bioRxiv - Plant Biology 2021Quote: ... Anti-rabbit IgG horseradish peroxidase-linked antibody (Promega, USA) was used as the secondary antibody at 1:10,000–20,000 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... The immunoblots were probed with appropriate secondary antibodies (Promega) and visualised by chemiluminescence (Supersignal detection kit ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... Secondary antibody (donkey α-goat HRP, Promega, 1:10,000). For His-tagged proteins ...
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bioRxiv - Immunology 2021Quote: ... incubated with anti-IgY secondary antibody (Promega Madison, USA) for an hour ...
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bioRxiv - Molecular Biology 2023Quote: ... and anti- mouse IgG antibody (1:1,000, #S3721, Promega).
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bioRxiv - Plant Biology 2023Quote: ... antibodies followed by anti-rabbit HRP conjugate (Promega, W4018). Protein loading was visualised by staining membranes with Ponceau S and protein signals were developed using the SuperSignal West Femto chemiluminescence kit (Pierce).
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bioRxiv - Developmental Biology 2024Quote: ... and secondary antibodies (anti-mouse HRP conjugate (Promega, W4028) and anti-rabbit HRP conjugate (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies (Anti-Rabbit/Mouse IgG HRP Conjugate, Promega) were used at 1:5000 in 5% Blotting-Grade Blocker (BioRad).
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bioRxiv - Molecular Biology 2023Quote: ... HRP conjugated secondary antibodies anti-mouse (Promega, PR-W4012) and anti-rabbit (Invitrogen 65-6120) ...
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bioRxiv - Biochemistry 2023Quote: ... and anti-mouse IgG alkaline phosphatase conjugated antibody (Promega). Band intensities were quantified by Image J ...
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bioRxiv - Bioengineering 2023Quote: ... followed by blotting with HRP-conjugated secondary antibodies (Promega). The protein bands were obtained with the ChemiDoc XRS+system (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies: anti-mouse-IgG (Promega, W4028, 1:2500) and anti-rabbit-IgG (Promega ...
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bioRxiv - Microbiology 2024Quote: ... and an anti-rabbit IgG HRP-conjugated antibody (Promega) in the iBlot system (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... Horseradish peroxidase-conjugated secondary antibodies were purchased from Promega and GE Healthcare and used with a 1:10,000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37 °C with Trypsin/Lys-C Mix (Promega, Charbonnières, France) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... proteins on the beads were digested by adding 400 ng trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were cleaved by overnight digestion in porcine trypsin (Sequencing Grade Modified, Promega, Wisconsin). The tryptic peptides were extracted by using 5% acetic acid in 50% acetonitrile and vacuum-dried by speedvac ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was carried out by adding 2 μg of trypsin solution (Promega, Charbonnières, France) to the alkylated proteins ...
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bioRxiv - Immunology 2021Quote: ... Total protein (100 μg) extracted from each sample was digested with Trypsin Gold (Promega, USA) with the ratio of protein:trypsin = 30:1 and incubated at 37 °C for 16 h ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were digested overnight at 37 °C using trypsin digestion (Sequencing Grade Modified Trypsin, Promega) at pH 8.0 using a protein to protease ratio of 100:1 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins from the gel pieces were digested with trypsin HPLC-grade (Promega AG, Dübendorf, Switzerland) overnight and the resulting peptide solutions were desalted on C18-based STAGE tips as previously described [43,44] ...
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bioRxiv - Biochemistry 2020Quote: A total of 100 μg protein was digested with Trypsin Gold (Promega, Madison, WI, USA) with the ratio of protein:trypsin = 30:1 at 37 °C for 16 h ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were subjected to on-filter proteolysis with Lys-C (Wako Chemicals) and trypsin (Promega). Tryptic peptides were eluted by centrifugation and acidified with trifluoroacetic acid prior to liquid chromatography-mass spectrometry (LC-MSMS ...
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bioRxiv - Physiology 2021Quote: ... the eluted proteins were digested with 10 μg/mL modified trypsin (Sequencing grade, Promega, USA) at 37 °C for 16 h ...
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bioRxiv - Plant Biology 2021Quote: ... Protein expression was confirmed by Western blot with Anti-HaloTag monoclonal Ab (1:2000) (Promega). Cy3-labeled and unlabeled dsDNA probes were generated with oligonucleotides listed in Supplemental Table 5 ...