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Citations for Promega :
751 - 800 of 4404 citations for 6 Hydroxy 2 Oxo 1 2 3 4 Tetrahydroquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Promega) and 6 µM ATP (UltraPure, Promega) in the kinase reaction buffer (40 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... by transient transfection with FuGENE 6 (Promega). On day 1 ...
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bioRxiv - Biochemistry 2022Quote: ... and transiently transfected with Fugene 6 (Promega), following the manufacturer’s instructions ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... Cells were transfected with Fugene-6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with Fugene-6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Fugene 6 was obtained from Promega (E2691). Lipofectamine 2000 was obtained from Thermo-Fisher Scientific (11668019) ...
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bioRxiv - Immunology 2023Quote: ... and lentivectors using Fugene 6 (Promega, E2691). 24 hours after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... FuGENE 6 transfection reagent (Promega, USA #E2691) diluted in serum-free DMEM was added to the plasmid mixture at 1.5:1 (reagent ...
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bioRxiv - Biochemistry 2024Quote: ... Transfections were performed with Fugene 6 (Promega) per manufacturer’s directions for 24-48 hours before selection with 400 μg/mL hygromycin B.
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bioRxiv - Biophysics 2023Quote: ... and FuGENE 6 transfection reagent (Promega, USA), propagated ...
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bioRxiv - Neuroscience 2023Quote: ... using Fugene 6 Transfection Reagent kit (Promega) according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... the two blots were hybridized O/N at 65 °C to each their radioactively labeled 3’ CF probe (AGO1 3’ CF and CSD2 3’ CF probes labelled with Prime-a-gene labeling kit from Promega). The membranes were washed 3 times in 2xSSC (0.3 M NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... overnight at 4°C and HRP-conjugated anti-rabbit antibody (1:2,000, W4011, Promega) for 1 hr at room temperature followed by ECL signal amplification and chemiluminescence detection (SuperSignal West Pico Chemiluminescent Substrate ...
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bioRxiv - Systems Biology 2023Quote: ... for 4 h at 37 °C and then with trypsin (Promega, 1:100, w:w) overnight at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Caspase-Glo 3/7 Reagent (Caspase-Glo® 3/7 Assay, Promega) was added to the wells ...
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bioRxiv - Cancer Biology 2022Quote: ... Caspase 3/7 activity was detected using Caspase-Glo 3/7 (Promega). Annexin V/Propidium Iodide staining was performed using Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... caspase-3/7 levels were examined using Caspase-Glo 3/7 (Promega) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... 0.4 μg envelope vector (pMD2.G) and 1.6 μg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...
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bioRxiv - Cell Biology 2021Quote: ... RPE1 cells were co-transfected with 1 µg KIF1C-mCherry-FRB and 0.5 µg FKBP-GFP-Myc-MAO using Fugene 6 (Promega) in fibronectin-coated glass-bottom dishes and imaged 24 hours later on an Olympus Deltavision microscope (Applied Precision ...
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bioRxiv - Immunology 2019Quote: ... 0.4 µg envelope vector (pMD2.G) and 1.6 µg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...
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bioRxiv - Bioengineering 2023Quote: ... PB-CAG-H2B-mCherry-IPuroR and pCMV-hyPBase were co-transfected into KhES-1 using Fugene 6 (Promega, E269A) transfection reagent following the manufacturer’s instruction ...
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: HEK293 IFITM3−/− cells were plated in a 6-well dish for 24 hours prior to transfection of IFITM3-pcDNA3.1 plasmid DNA using FuGene 6 reagent (Promega) at a ratio of 3:1 ...
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bioRxiv - Microbiology 2021Quote: ... or 3) Pepsin (Promega). 1 ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µL RNasin (Promega) and 100 µL lysis buffer for a total volume of 300 µL for IP by rotation for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... First-strand cDNA was synthesized from 2 µg of total RNA using random hexamers and MMLV reverse transcriptase (Promega). The cDNA was used as a template for quantitative PCR performed using Syber Green reagent (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... and SREBP-2 (NM_004599) expression plasmids were prepared by subcloning PCR products into a mammalian expression plasmid pCI (Promega). Likewise ...
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bioRxiv - Molecular Biology 2021Quote: ... before cells were permeabilized using 0.2% Triton X-100 PBS for 10 min at room temperature, then blocked for 30 min (2% BSA / 0.5% fish skin gelatine / PBST.5, 2U/µL RNAsin Plus (Promega) at room temperature) ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 μl of f/2 and 10X concentrated substrate (10 mM firefly luciferin, 1,000X dilution of NanoLuc Dual-Glo reagent, Promega) were added to the respective wells.
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bioRxiv - Cancer Biology 2019Quote: ... then washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg/μl sequencing grade trypsin (Promega). Tryptic peptides were eluted off ...
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bioRxiv - Biochemistry 2020Quote: ... the samples were brought to room temperature for 5-10 minutes while 2 mL of 30 mM coelenterazine (Promega), or 15 mL of 1-to-50 diluted NanoLuc substrate (Promega) ...
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bioRxiv - Bioengineering 2020Quote: ... the Nbs-virus mixture was removed from Vero E6 cells followed by 500 μL of DMEM with 2% FBS and 0.9% carboxymethyl cellulose (Promega) was overlaid ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was synthesized using 5μg of total RNA at 42 °C for 2 hours using avian myeloblastosis virus (AMV) reverse transcriptase (Promega). A 20 µL reaction containing 10 ng or 10 pg (for 16S rRNA ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was inserted into the multiple cloning site (MCS) of the psicheck-2 vector (supplied by Promega) downstream of the Renilla luciferase gene according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... alkylated using 50 mM iodoacetamide for 2 hrs at ambient temperature and then digested with 1.2 μg proteomics-grade trypsin/LysC (Promega) according to the SP3 protocol (27) ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected into HeLa cells for 24 h (2 μg of cDNA/9.6 cm2 plate) using Fugene (Promega). For gene silencing ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were diluted to a final concentration of 500,000 cells/mL in Expi293 expression medium plus 2% (v/v) Glosensor reagent (Promega) and incubated for 75 minutes at room temperature with gentle rotation ...
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bioRxiv - Microbiology 2022Quote: ... Samples were digested by adding 100 µL trypsin solution (10 mM TEAB, pH 7.5, 2 µg Promega trypsin #V5111) and incubated overnight at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... while the complementary sequence was cloned downstream of the Renilla luciferase reporter cassette in a psiCHECK-2 plasmid (Promega). The same target sequence was also cloned downstream of a TagRFP reporter cassette in an ePB-BSD-TT piggyback vector (see plasmids ...
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bioRxiv - Plant Biology 2021Quote: ... benthamiana leaves were harvested 2-days after infection and then applied to a Dual-Luciferase Reporter Assay System (Promega) to detect the activation of the ACS2 and ACS2mut promoters by NOR ...
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bioRxiv - Neuroscience 2020Quote: ... The first strand cDNA was synthesized from 2 μg RNA with GoScript™ Reverse Transcriptase (Promega, Madison, WI, USA).
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bioRxiv - Cell Biology 2021Quote: ... and a total amount of 2 μg RNA was reverse transcribed with a GO Script Reverse Transcriptase kit (Promega) using random primers ...
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bioRxiv - Cell Biology 2022Quote: ... 2 hours post-transfection media was replaced with fresh complete DMEM containing 646 HALO Dye (20 nM; GA112A; Promega). The next day ...
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bioRxiv - Genomics 2022Quote: ... 2 µl of yeast lysate was used as a template in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Neuroscience 2022Quote: ... or 1.0 μg/side of MEK inhibitor 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]butadiene (U0126; Promega, Madison, WI); 0.5 ...
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bioRxiv - Genomics 2019Quote: ... 2 μL of extracted genomic DNA was added to 25 μL of GoTaq Green Master Mix (Promega, Madison, WI) and 1 μL of 10 μM universal primers 8F (5’- AGAGTTTGATCCTGGCTCAG-3’ ...
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bioRxiv - Biochemistry 2019Quote: ... the urea concentration of each sample was diluted to ∼2 M by adding 100 mM ammonium bicarbonate pH = 8.0 followed by addition of trypsin (Promega) at a final ratio of 1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were trypsinised on the beads in 60 μl of Buffer I (2 M urea, 50 mM Tris-HCl pH 7.5, 5 μg/ml Trypsin [modified sequencing-grade trypsin; Promega]) for 30 min at 37°C in a thermomixer ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcriptase reaction was performed from 2 µg of total RNA by using ImProm-IITM Reverse Transcription System (Promega). Aliquots of cDNA obtained were amplified in PCR and quantitative real-time PCR reactions ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 ml (approximately 2 × 109 cells) were used for gDNA extraction with the Wizard Genomic DNA Purification kit (Promega), which typically yielded 50 μl of 0.5-1.5 μg/μl gDNA.
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bioRxiv - Microbiology 2020Quote: ... The plate was incubated in the dark for 2 mins then read on a Glomax Multi+ Detection System (Promega) as above ...