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Citations for Promega :
751 - 800 of 3805 citations for 6 Bromo 3 4 dihydro 2H 1 benzopyran 3 methanamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: HEK293 cells growing at 70% confluency in a 10 cm dish were transfected with wild-type human NOPR or NOPLight (3 µg DNA) and GloSensor-20F (Promega, 2.5 µg DNA) using 12 µL Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... After five-fold dilution with 50-mM ammonium bicarbonate, the sample was digested using Lys-C (Wako, Osaka, Japan) at 37°C for 3 h following trypsin (Promega, Madison, WI, USA) at 37°C for 16 h ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... membrane integrity (CytoTox-OneTM homogeneous membrane integrity assay kit: lactate dehydrogenase (LDH release) and caspase activity/ apoptosis (Caspase-Glo® 3/7 assay system kit) (all from Promega, WI, USA), as described previously17 (see Supplementary data for detailed description).
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bioRxiv - Evolutionary Biology 2023Quote: ... The amplicons generated from the 5’ and 3’ race PCRs were subjected to purification and subsequent cloning into the pGEM-T easy vector (Promega Corp., WI, USA). The constructed vectors were sequenced (Plasmidsaurus ...
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bioRxiv - Bioengineering 2024Quote: ... 100 uL of the diluted lysate was added to each well along with an equal volume of Caspase Glo 3/7 assay reagent (Promega, Madison, Wisconsin, USA), according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using 5 μl of the eluent as template using the U3 antisense primer 5’ TGTGTGGTAGATCCACATCG-3’ and M-MLV RT RNase [H-] (Promega, WI. Cat. No. MR3681) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then co-transfected with either 0.3 μg/well of Smad4 firefly luciferase reporter plasmid constructs (pLuc366 or pLuc207) or the control pGL3-Basic vector (Promega, San Luis Obispo, USA). The renilla luciferase plasmid was also co-transfected to correct for variations in transfection efficiency (45 ng/well) ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were transfected with 1 μg of DNA (1 μg of an L1-expressing plasmid or 0.5 μg of the L1-expressing plasmid and 0.5 μg of either a pCMV-3Tag-8-Barr control or ISG-expressing plasmid) using 3 µL of FuGENE HD transfection reagent (Promega, Madison, WI, United States) and 100 µL of Opti-MEM (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... the pHAD plasmid was linearized by PmeI enzyme to expose the ends of the 5’ and 3’ inverted terminal repeats (ITRs) and transfected into 116 producer cells (Profection® Mammalian Transfection System, Promega, Madison, WI, USA). Helper virus (HV ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... Media was changed at 3 hpi and luciferase luminescence was measured at 24 hpi using the Nano-Glo® Luciferase Assay (Promega, Madison, USA, #N1130) kit ...
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bioRxiv - Biochemistry 2022Quote: ... were transfected using FuGene 6 (Promega). The supernatant containing V0 virus (virus passage zero (progeny virus) ...
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bioRxiv - Immunology 2022Quote: ... using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... using FuGene 6 transfection reagent (Promega). The medium was changed 18 h after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... and 1.2 µl Fugene 6 (Promega) transfection mix and incubated for 15min at room temperature before adding to HeLa cells plated in one well of a 24 well plate ...
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bioRxiv - Cell Biology 2021Quote: ... FuGENE 6 reagent (Promega, Madison, WI) was used for transient transfection of HeLa cells according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... S7A–C using FuGene 6 (Promega). Two days after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... 8μL of Fugene-6 (Promega, E2691) was mixed with various amounts of plasmid DNA and allowed to incubate at room temperature for 15 minutes before addition to cells ...
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bioRxiv - Cell Biology 2021Quote: ... FuGENE® 6 Transfection Reagent (Promega); Luminata Forte Western HRP substrate (Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... MDCK) or with FuGENE 6 (ProMega) (HeLa) ...
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bioRxiv - Cancer Biology 2021Quote: ... using FuGENE 6 Transfection Reagent (Promega) according to manufacturer protocol ...
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bioRxiv - Genomics 2023Quote: ... and transfected (Fugene 6, Promega # E269A) with pBABE-puro SV40 LT plasmid (Addgene # 13970 ...
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bioRxiv - Immunology 2023Quote: ... using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM CaCl2) and incubated for 4 hours with 1 µg trypsin (Promega) at 37°C and shaking at 1200 rpm ...
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bioRxiv - Neuroscience 2020Quote: ... pCAG-DCC:TDTOMATO and pCAG-DCCkanga:TDTOMATO plasmids (1 µg) were transfected into the plated U251 cells using FuGENE 6 (Promega) in Opti-MEM (Gibco ...
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bioRxiv - Immunology 2020Quote: ... were transfected with 4.5 µg of the pBabe-puro-NCAM140 mApple construct (NCBI reference sequence: NM_000615.6, transcript variant 1) using Fugene 6 Transfection Reagent (Promega). Supernatant containing viral particles was collected and concentrated using PEG-IT (System Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
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bioRxiv - Developmental Biology 2022Quote: ... were transfected into NCCIT cells (ATCC) at ∼70% confluency using a 6:1 ratio of Fugene HD (Promega) for 48 hours in ATCC-formulated RPMI media ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested off the beads by adding 6 μL of 1 μg/μL sequencing grade Trypsin (Promega), diluted in 50 mM acetic acid ...
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bioRxiv - Cell Biology 2021Quote: ... Donor 293T cells were co-transfected with 1.5 µg of plasmids encoding different untagged S mutants and 0.6 µg of pmCherry-N1 using 6 µL of Fugene 6 following the manufacturer’s instructions (Promega). Acceptor Vero cells expressing human ACE246 ...
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bioRxiv - Microbiology 2020Quote: ... Donor cells were co-transfected with 1.5 μg pCAGGS-S and 0,5 μg pmCherry-N1 using 6 μl of Fugene 6 following the manufacturer’s instructions (Promega). Acceptor cells were treated with CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate ...
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bioRxiv - Cell Biology 2020Quote: ... with Fugene 6 transfection reagent (Promega E2691). 10 mM sodium butyrate was then added to the transfected cells 3 hours post transfection for an overnight incubation at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfection was performed with Fugene 6 (Promega). Supernatant was filtered at 0.45 µm before storage at −80°C For stable expression of TRIM21 ...
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bioRxiv - Molecular Biology 2021Quote: ... and pCIG2 UBE3AT485A using Fugene 6 (Promega). 4 hours post transfection cells were treated with chemical libraries ...
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bioRxiv - Cell Biology 2019Quote: ... polyethylenimine “MAX” (polysciences) or Fugene 6 (Promega) were used ...
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bioRxiv - Cancer Biology 2019Quote: ... FuGENE 6 (Promega, E2691, Madison, WI, USA) was used ...
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bioRxiv - Molecular Biology 2022Quote: ... or FuGENE® 6 (Promega Cat# E2961) as transfection reagents ...
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bioRxiv - Synthetic Biology 2021Quote: ... Fugene 6 transfection reagent (Promega, WI, USA) was used for transient transfection according to the manufacturer’s instructions ...
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A rare variant on a common risk haplotype of HFE causes increased risk of hereditary hemochromatosisbioRxiv - Genetics 2019Quote: ... or HEK293 cells using Fugene 6 (Promega) following manufacturer’s instructions and studied 48-72 hours post-transfection.
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bioRxiv - Developmental Biology 2020Quote: ... using FuGene 6 (Promega, catalog no. E2691). 24 hrs post-transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Promega) and 6 µM ATP (UltraPure, Promega) in the kinase reaction buffer (40 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... by transient transfection with FuGENE 6 (Promega). On day 1 ...
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bioRxiv - Biochemistry 2022Quote: ... and transiently transfected with Fugene 6 (Promega), following the manufacturer’s instructions ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... Cells were transfected with Fugene-6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with Fugene-6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... FuGENE 6 transfection reagent (Promega, USA #E2691) diluted in serum-free DMEM was added to the plasmid mixture at 1.5:1 (reagent ...
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bioRxiv - Immunology 2023Quote: ... and lentivectors using Fugene 6 (Promega, E2691). 24 hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: Fugene 6 was obtained from Promega (E2691). Lipofectamine 2000 was obtained from Thermo-Fisher Scientific (11668019) ...
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bioRxiv - Biochemistry 2024Quote: ... Transfections were performed with Fugene 6 (Promega) per manufacturer’s directions for 24-48 hours before selection with 400 μg/mL hygromycin B.
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bioRxiv - Biophysics 2023Quote: ... and FuGENE 6 transfection reagent (Promega, USA), propagated ...