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Citations for Promega :
751 - 800 of 4085 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Generation of infectious lentiviral particles and subsequent cell transduction was performed as previously described.26 FuGENE 6 (Promega) was used to transfect select plasmids ...
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bioRxiv - Microbiology 2021Quote: ... 293T cells were co-transfected with 1.5 mg of spike expression plasmids in pCDNA3 and 0.5 mg pmCherry-N1 using Fugene 6 and following the manufacturer’s instructions (Promega). Vero cells were treated with CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate ...
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bioRxiv - Cell Biology 2021Quote: ... zyxin(-/-) MEFs were transfected with 5 μg total DNA using the Fugene® 6 transfection reagent (E2691, Promega) and plated on glass coverslips.
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bioRxiv - Molecular Biology 2021Quote: ... 32μg SIV4+ (49)and with or without 1μg of pcDNA3.1 Vpx expression plasmid using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were co-transfected with 0.5 μg of spike expression plasmids in pCDNA3 using Fugene 6 and following the manufacturer’s instructions (Promega). Cell-cell fusion was measured using an Incucyte and determined as the proportion of green area to total phase area ...
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bioRxiv - Cell Biology 2020Quote: ... and the solution was added to OMEM (75 μL) containing 6 μL Fugene HD reagent (Promega, Madison, WI). The mixture was incubated at room temperature for 10 minutes and gently added to the growth medium on the cultured cells ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated for 24 h and viability was measured following a 6 h incubation with CellTiterBlue (Promega). Viability was quantified by fluorescent measurement (Ex ...
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bioRxiv - Cancer Biology 2022Quote: ... 1mg of the plasmid was transfected to 293T cells (ATCC #CRL-11268) using FuGENE 6 Transfection Reagent (Promega) to produce the retroviruses ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with 8 µg of pcDNA3.1-GFP-mNLS-K145Q using Fugene 6 Transfection reagent (Promega, E2692) per manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: Lentiviral particles were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... DF1 cells were transfected with the different RCAS retroviral plasmids using FuGENE 6 Transfection reagent (Promega, Cat. E2691), accordingly to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with plasmid DNA by the calcium phosphate or lipofection method using FuGene 6 (Promega, WI) (Yoshida et al. ...
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bioRxiv - Neuroscience 2023Quote: HEK293T cells were transfected with either Tau-encoding or control constructs using Fugene 6 (Promega, cat. no. E2691) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was assessed 6 days post-treatment using a Cell-TiterGlo 3D Cell Viability assay (Promega, G9683), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with LDLR-GFP (WT, C75W, and C75W/C34W) for 48 h with Fugene 6 (Promega). Cells were starved in 5% LPDS media (Millipore Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... precipitated proteins were resuspended in 0.1% RapiGest with 6 μL of sequencing-grade trypsin (Promega, 0.5 μg/μL) added to each sample and digested overnight at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... methylase was amplified using M.SssI_HindIII forward and reverse primers (Sup Table 6) cloned into pGEMT-Easy (Promega, USA) and sequenced for verification ...
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bioRxiv - Genetics 2024Quote: ... Effects on endogenous FAS exon 6 inclusion were determined by PCR using GoTaq flexi DNA polymerase (M7806, Promega) and the following primers:
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bioRxiv - Molecular Biology 2024Quote: Cells were seeded in 12-well plates at a density of 200,000 per well and transfected using 1.5 µl/well FuGENE 6 (Promega) with 5 ng of CMV-Renilla plasmid (pTG888) ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µl RNasin Plus (Promega, catalog no. N2615), 0.8 µl 100 mM ATP (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... using 2 µg of sequencing grade trypsin (Promega) per fraction 55 ...
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bioRxiv - Biochemistry 2022Quote: ... either 2 µg pGL4.47[luc2P/SIE/Hygro] (Promega) or pGL4.52[luc2P/STAT5RE/Hygro] (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... 2) pRL Renilla luciferase control reporter (Promega, E2241) and 3 ...
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bioRxiv - Cancer Biology 2021Quote: ... pGL4.10 (Luc.2; E665A) was purchased from Promega. The pGL4.10-ITGB1 reporter and a control Renilla luciferase vector were co-transfected into breast cancer cell lines using Lipofectamine 3000 transfection kit (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... plus 2 uL of random primers (Promega, C1181). This was then incubated at 70 °C for 10 mins ...
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bioRxiv - Biochemistry 2022Quote: ... 2 U/μl of RNasin Ribonuclease Inhibitor (Promega)) for 20 min on ice ...
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bioRxiv - Microbiology 2022Quote: ... 2 µM dNTPs and MMLV reverse transcriptase (Promega). Primer extension reactions to analyze initiation from the +1 and +3 sites of the le promoter were subjected to electrophoresis in 6% polyacrylamide gels containing 7 M urea in Tris-borate-EDTA buffer ...
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bioRxiv - Biochemistry 2023Quote: Cell viability assays using CellTiter-Glo 2 (Promega) were performed according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... we used CellTiter Glo 2 assay systems (Promega). Approximately 2.0 × 103 cells/well were seeded in a 96-well plate and incubated in RPMI1640 culture medium over 4 days at 37 °C and 5% CO2 ...
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bioRxiv - Systems Biology 2023Quote: ... 2 µl of 5x MTase-Glo Reagent (Promega) was added to wells ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 2 µL of sequencing grade modified trypsin (Promega) at a concentration of 0.1 µg/μL and 23 µL of 50mM ammonium bicarbonate/0.01% ProteaseMax were added ...
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bioRxiv - Microbiology 2023Quote: ... 2 µL of 100 bp DNA Ladder (Promega) was added to confirm amplicons size and run at 100 V for 45 mins ...
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bioRxiv - Molecular Biology 2023Quote: ... and treated with 2 μg trypsin (Promega, v511c), shaking overnight at 700 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... using the CellTiter-Blue assay (G8081/2, Promega) following the protocol of the manufacturer using an EnVision multimode plate reader (PerkinElmer) ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µL ROX (CRX reference dye, Promega, C5411) were added to 1 mL SybrGreen as a passive reference dye that allows fluorescent normalization for qPCR data ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µL ROX (CRX reference dye, Promega, C5411) were added to 1 mL SybrGreen as a passive reference dye that allows fluorescent normalization for qPCR data ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μg/ml of herring sperm DNA (Promega) in dilution solution ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µL ROX (CRX reference dye, Promega, C5411) were added to 1 mL SybrGreen as a passive reference dye that allows fluorescent normalization for qPCR data ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µg of sequencing grade modified trypsin (Promega) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... the dual luciferase reporter psiCHECK-2 vector (Promega) was used ...
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bioRxiv - Biochemistry 2024Quote: ... and 2 EDTA-free Protease inhibitor tablet (PROMEGA)) via probe sonication (10s ON/ 10s OFF at 60% amplitude ...
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bioRxiv - Biochemistry 2020Quote: ... Digestion solutions were brought to a final concentration of 2 M urea and 2 mM CaCl2 and a second digestion was performed overnight at 37 °C using trypsin (Promega, v5280) at 1:100 w/w ...
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bioRxiv - Cell Biology 2021Quote: ... Protein material was reduced with tris(2-carboxyethyl) phosphine (TCEP; 10 mM final) and digested overnight with 2 μg sequence-grade modified trypsin Gold (Promega, V5280) in 50 mM ammonium bicarbonate (NH4HCO3 ...
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bioRxiv - Microbiology 2021Quote: ... rSARS-CoV-2/Δ7a-Nluc or rSARS-CoV-2/Nluc-2A infected cells was quantified using Nano-Glo® Luciferase Assay System (Promega) following the manufacturers’ specification.
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bioRxiv - Immunology 2022Quote: Replication competent HIV were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 3 ug of pCAG-NLS-Bxb1 was diluted in 250 uL of OptiMEM and 6 uL of Fugene (Promega). On day 2 ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for an additional 6 hours at 37°C before 75μl of Bio-Glo Luciferase Assay Reagent (Promega) was added to each well ...
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bioRxiv - Molecular Biology 2021Quote: ... All the clones were transfected into the HEK-293T cells at 90% confluency using FuGene 6 as transfection reagent according to the manufacturer’s instructions (Promega). dsiRNAs targeting Rpp30 (10 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... Fresh bacmid DNA was transfected into Sf9 cells at 0.5×106 cells/mL in 6-well cell culture plates using FuGene HD (Promega) according to the manufacturer’s protocol (final concentration 10 μg/mL) ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells were transfected with WT or mutant C10 constructs using the FuGENE 6 transfection reagent (Promega, cat# PRE2691). The cells were incubated at 37 C at least overnight before downstream analysis.