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Citations for Promega :
701 - 750 of 1259 citations for Usutu Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... PsiYAB11 protein was expressed using the TNT SP6 Coupled Wheat Germ Extract System (Promega). The protein-bound beads were incubated with 50 ng of adaptor-ligated gDNA fragments on a rotator for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: In-gel protein digestion of every spot was performed using sequencing-grade trypsin (Promega) as described elsewhere (26) ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were digested overnight at 37°C using MS-grade trypsin (Promega, Cat# V5280). The next morning ...
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bioRxiv - Neuroscience 2022Quote: ... Protein enzymatic cleavage was carried out with trypsin (1:20, w/w; Promega, V5280) at 37 °C for 16 h ...
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bioRxiv - Molecular Biology 2022Quote: Fifty μl protein expression reaction using TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600) containing 1000 ng pIX-Halo-bZIP plasmids was incubated for 3 h at 30°C ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were precipitated with acetone and trypsin-digested using sequencing grade-modified trypsin (Promega). The resulting peptide mixture was purified by STAGE-TIP method using a C18 resin disk (3M Empore ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins eluted at pH 9.0 were further treated with 5 U of CIAP (Promega) and the buffer provided by the manufacturer (a negative control was prepared without phosphatase) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The proteins were digested with sequencing-grade modified trypsin (Promega Corporation, Madison, WI, USA). The peptides were analyzed using a nano LC–MS system (UltiMateTM 3000 RSLCnano and Orbitrap ExplorisTM 240 ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested for 18 h with trypsin/Lys-C Mix (Promega Corporation, USA) and 10 μg of each sample was used for proteome analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were digested with chymotrypsin (5 ng/μL) at 25ºC overnight (Trypsin gold, Promega). Digestion was stopped by addition of 5% formic acid and peptides extracted twice with 70% acetonitrile and 5% formic acid (10 min sonication) ...
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bioRxiv - Molecular Biology 2022Quote: ... and the proteins were digested with 1/100 (w/w) trypsin (Promega, Cat # V5111) overnight at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... dark) and digestion using 0.015 µg Trypsin for 1.5 µg protein (Trypsin Gold, Promega, Fitchburg ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested by trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega; protein:enzyme (w/w) ratio – 100:1 ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were then digested into peptides with sequencing grade modified trypsin (Promega V5113) at 37 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
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bioRxiv - Biochemistry 2023Quote: Eluted proteins were digested with Lys-C (FUJIFILM Wako) and trypsin (Promega, Madison, WI) according to the PTS protocol21 ...
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bioRxiv - Biophysics 2023Quote: ... the desired volume of protein solution was incubated with Factor Xa (Promega, Madison, WI). All experiments were carried out in a tris buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were further digested by the addition of 2 μg trypsin (Promega #V5113) for 4 hours at room temperature on a shaker ...
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bioRxiv - Physiology 2023Quote: ... Chemiluminescent detection of in vitro translated protein was performed following the manufacturer’s protocol (Promega).
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bioRxiv - Biochemistry 2024Quote: ... The HaloTag-JF503-SWSAP1 was quantified by using recombinant HALO-GFP protein standard (Promega) pre-incubated with JF503 at a 1:1 molar ratio and titrated into single molecule buffer (20 mM Tris pH7.5 ...
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bioRxiv - Microbiology 2024Quote: ... Prior to tryptic digestion of the protein overnight using mass-spec grade Trypsin (Promega), protein was reduced and alkylated using dithiothreitol and iodoacetamide ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were digested using filter-aided sample preparation with sequence-grade trypsin (Promega, # V5111) at 37°C overnight ...
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bioRxiv - Immunology 2024Quote: ... with a 1:50 ratio of purified protein to Endoglycosidase H (Endo H) (Promega). After treatment ...
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bioRxiv - Bioengineering 2024Quote: ... The reactions were performed using the S30 T7 High-Yield Protein Expression System (Promega). 3 μl of peptide solution were added to reaction mixtures to get a final concentration respectively of 32 μg/ml or 512 μg/ml in a final volume of 15 μL in nuclease-free PCR tubes ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were resuspended in 100 mM NH4HCO3 and digested with trypsin (Promega, UK) at an enzyme-to-substrate ratio of 1:20 and protease max 0.1% (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... the blotted proteins were developed using Nano-Glo® In-Gel Detection System (Promega) and scanned with a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng/μl of mRNA and 6 ng/μl QuantiLum Recombinant Luciferase protein (Promega) were coinjected ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37 °C with Trypsin/Lys-C Mix (Promega, Charbonnières, France) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... proteins on the beads were digested by adding 400 ng trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were cleaved by overnight digestion in porcine trypsin (Sequencing Grade Modified, Promega, Wisconsin). The tryptic peptides were extracted by using 5% acetic acid in 50% acetonitrile and vacuum-dried by speedvac ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was carried out by adding 2 μg of trypsin solution (Promega, Charbonnières, France) to the alkylated proteins ...
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bioRxiv - Immunology 2021Quote: ... Total protein (100 μg) extracted from each sample was digested with Trypsin Gold (Promega, USA) with the ratio of protein:trypsin = 30:1 and incubated at 37 °C for 16 h ...
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bioRxiv - Cell Biology 2019Quote: ... and RXRA proteins were produced using the TNT® Quick Coupled Transcription/Translation System (Promega). Fluorescent probes were synthesized by Integrated DNA Technologies and corresponded to the LXRE1 sequence with IRDye 700 attached to the 5’ end ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were digested overnight at 37 °C using trypsin digestion (Sequencing Grade Modified Trypsin, Promega) at pH 8.0 using a protein to protease ratio of 100:1 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins from the gel pieces were digested with trypsin HPLC-grade (Promega AG, Dübendorf, Switzerland) overnight and the resulting peptide solutions were desalted on C18-based STAGE tips as previously described [43,44] ...
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bioRxiv - Biochemistry 2020Quote: A total of 100 μg protein was digested with Trypsin Gold (Promega, Madison, WI, USA) with the ratio of protein:trypsin = 30:1 at 37 °C for 16 h ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were subjected to on-filter proteolysis with Lys-C (Wako Chemicals) and trypsin (Promega). Tryptic peptides were eluted by centrifugation and acidified with trifluoroacetic acid prior to liquid chromatography-mass spectrometry (LC-MSMS ...
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bioRxiv - Physiology 2021Quote: ... the eluted proteins were digested with 10 μg/mL modified trypsin (Sequencing grade, Promega, USA) at 37 °C for 16 h ...
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bioRxiv - Plant Biology 2021Quote: ... Protein expression was confirmed by Western blot with Anti-HaloTag monoclonal Ab (1:2000) (Promega). Cy3-labeled and unlabeled dsDNA probes were generated with oligonucleotides listed in Supplemental Table 5 ...
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bioRxiv - Cell Biology 2021Quote: ... The cleared supernatant was added to a column containing HisLink Protein Purification Resin (V8823, Promega) and his-tag fusion proteins were purified according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Proteins in each well were digested with 1 μg of sequencing-grade trypsin (Promega Inc.) in 75 μl of 50 mM ammonium bicarbonate and incubated overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... On-bead protein digestion was performed by adding 1 µg of sequencing grade trypsin (Promega) to the samples ...
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bioRxiv - Plant Biology 2021Quote: ... Protein preparation and luciferase– substrate interaction were conducted with a Luciferase Assay Report Kit (Promega). Luciferase activity was measured by a microplate reader (Thermo Scientific Varioskan Flash) ...
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bioRxiv - Systems Biology 2020Quote: ... Treated proteins were digested overnight at 37 °C using sequencing-grade trypsin (Promega, cat: V511C) at an enzyme-to-protein ratio of 1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins extracted were then processed with a precipitation-based digestion protocol using trypsin (Promega) at a ratio of 1:20 as described previously(Collins et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and proteins were digested on-bead with trypsin (1.5 µg, in provided buffer, Promega Corp.) overnight at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: Halo fusion proteins were visualized in cells by staining with fluorescent Halo ligand (Promega GA1110). Cells were seeded on polyD lysine coated coverslips (neuVitro ...
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bioRxiv - Cell Biology 2022Quote: ... proteins in the plugs where digested overnight with Trypsin (10μg/ml) (Promega, Madison, WI, USA) at 37°C in a 25-mM NH4HCO3 buffer (0.2 µg trypsin in 20 µL) ...
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bioRxiv - Microbiology 2021Quote: ... Protein digestion was performed on beads using Trypsin Gold (MS grade; Promega, cat. no. V5280), as previously described in (Hughes et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were digested overnight at 37 °C using sequencing grade trypsin (100 ng; Promega) in a buffer containing 25 mM 4-ethylmorpholine acetate and 5% MeCN ...