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Citations for Promega :
701 - 750 of 790 citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: The potential for M2e-specific antibodies to induce ADCC was evaluated using a mouse FcγRIV ADCC Reporter kit (Promega). In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was a horseradish peroxidase polyclonal goat anti-mouse (from 1:5,000 to 1:10,000, depending on the primary antibody; Promega, W4021). Proteins were detected with the ECL chemiluminescence reagent (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell numbers were quantified using a colorimetric 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS)-based CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The kinase activity assay was performed with 4 µl beads in a 384-well plate using the ADP-Glo™ Kinase Assay (Promega, Madison, WI). The luminescence of each well was read using a Synergy™ H1 Hybrid Multi-Mode Microplate Reader (BioTek Instruments ...
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bioRxiv - Microbiology 2020Quote: ... collected by centrifugation (17,000 g for 10 min at 4°C) and solubilized in 20 μl 50 mM TEAB containing 0.2 % ProteaseMAXTM Surfactant (Promega UK Ltd, Cat. # V2071) for 1-2 h with vortex and occasional sonication ...
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bioRxiv - Cell Biology 2020Quote: ... washed again in DPBS and stained with 1mg/ml X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside, Promega, Madison, WI, US) in DMF (Dimetil formamide ...
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bioRxiv - Immunology 2020Quote: Activity of the inflammatory caspases 1/4/5 was measured using a commercially available Caspase-Glo® 1 Inflammasome Assay (Promega, WI, USA) from HFFs seeded in 96-well plates (2×104 cells per well) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... PHH were infected with HepAD38-derived GTD virus at 500 viral genome equivalents per cell in maintenance medium supplemented with 4 % PEG 8000 (Promega, Madison, WI; V3011). For patient sera infections ...
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bioRxiv - Microbiology 2019Quote: ... Supplementary Table 4) and DNA sequencing of the products following clean-up (Wizard SV Gel and PCR Clean-Up System, Promega, Southampton, Hampshire, UK) using dye terminator chemistry (Eurofins ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay (Celltiter 96 Aqueous One-solution Cell Proliferation Assay, Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... then dipped in a well of a 96-well PCR plate filled with 20 µl of a gyrB PCR mix (4 µl of 5x GoTaq buffer (Promega, Madison, WI, USA), 0.4 µl of 10mM dNTP ...
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bioRxiv - Biochemistry 2023Quote: ... MTS ([3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (#G3581) was purchased from Promega (Charbonnières-les-Bains, France).
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bioRxiv - Microbiology 2023Quote: ... and the reaction was revealed with a solution of NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate) (Promega, Madison, WI, USA).
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bioRxiv - Microbiology 2023Quote: ... were not screened for Wolbachia.All PCRs were performed in a final reaction volume of 20 μl including 4 μl of 5X reaction buffer (Promega, Madison, WI, USA), 1.5 μl of dNTPs (2.5 mM) ...
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bioRxiv - Biochemistry 2023Quote: ... and 20 µL of 1:1000 (diluted in phenyl red-free DMEM with 4% FBS) HaloTag™ NanoBRET™ 618 Ligand (Promega G9801) was added to each well ...
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bioRxiv - Biochemistry 2024Quote: ... Kc167 cells were seeded in 6-well plates and transfected (3 µg DNA per well) with the JHRE reporter or control (EGFP-pIEx-4) plasmids using the standard FuGENE-HD transfection protocol (Promega, Madison, WI, USA). About 48 h post-transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and further incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... washed three times (10 minutes each) with PBST before incubation for 1 h with α-Mouse IgG HRP Conjugate (Promega) secondary antibody (1:10 000 in PBST) ...
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bioRxiv - Neuroscience 2020Quote: 1μg of total RNA from the human and mouse brain specimen was reverse-transcribed using the M-MLV reverse transcriptase (Promega) for efficient synthesis of the first-strand cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-mouse HRP conjugate (W4021, 1:10,000) and Anti-Rabbit HRP Conjugate (W4011, 1:10,000) were purchased from Promega (Madison, WI). Anti-COPI antibody was a gift from Charles Barlowe (Dartmouth Univ ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and were then incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: A DNA fragment bracketing the proximal promoter and transcription start site of the Iqsec3 gene (–280 to +36) was PCR-amplified from mouse genomic DNA and subcloned into the pGL3-Basic vector (Promega). Full-length mouse Npas4 cDNA was subcloned in pcDNA3 (Gibco-Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... antibodies before probing with horseradish peroxidase (HRP)-conjugated anti-rabbit (for H3) or anti-mouse (for anti-GFP and anti-TUB) as the secondary antibody (1:5000; Promega, Madison ...
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bioRxiv - Molecular Biology 2021Quote: ... Total genomic DNA extracted from liver mouse and HEK293 cells were used as templates for PCR-cloning of the mouse/human promoter/intron fragments into the KpnI/MluI sites of pGL3 basic luciferase reporter vector (Promega). The glutamic acid (E ...
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA from Proteinase K-digested mouse toes was extracted using the Wizard SV genomic DNA extraction kit (Promega, A2360). PCR reactions to amplify a fragment containing the target sites were carried out using the forward and reverse primers (Forward primer ...
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bioRxiv - Genomics 2021Quote: ... Membranes were washed three times in TBS-T before incubating with secondary antibodies (HRP-anti Mouse-IgG (H+L) (Promega)) for 45 min in TBS-T and 2% milk ...
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bioRxiv - Cell Biology 2020Quote: ... HRP Conjugate (W4011, 1:5000), and anti-Mouse IgG (H+L), HRP Conjugate (W4021, 1:5000) were purchased from Promega.
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bioRxiv - Neuroscience 2022Quote: DNA constructs with different WT and mutated 5’UTRs of mouse Sox2 mRNA were assembled in the psiCheck-2 vector (Promega) including synthetic Renilla luciferase gene (hRluc ...
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bioRxiv - Cancer Biology 2022Quote: ... The blots were incubated with secondary antibody (horseradish peroxidase-conjugated goat anti-mouse (Cat# W402B) or goat anti-rabbit IgG (W401B) from Promega, Madison ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse DNA was extracted from the ear using the Wizard® Genomic DNA Purification Kit from Promega (Madison, WI, USA), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega, #W4021) used at a 1:10,000 dilution.
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bioRxiv - Microbiology 2020Quote: ... the plates were washed with PBS-T five times and added with a 1:5000 dilution of HRP-conjugated goat anti-mouse IgG antibody (Promega) in blocking buffer at 37 °C for 45 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Reporter plasmids were constructed by inserting the mouse Olig2 gene mR2 and mR6 regions identified by the ChIP assays in situ into the pGL3-Basic vector (Promega), respectively ...
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bioRxiv - Cell Biology 2021Quote: The luciferase reporter plasmid was generated by cloning the mouse HDAC7 promoter into the NheI site of pGL4 Luciferase reporter plasmid (Promega). The mouse HDAC7 promoter was amplified using Platinum SuperFi II DNA Polymerase (12361010 ...
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bioRxiv - Immunology 2020Quote: ... were used as primary antibodies and horseradish peroxidase (HRP)-conjugated secondary antibodies were from Promega (Rabbit W401B and Mouse W402B), and the substrate ECL was detected by Pierce ECL2 solution (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: OCA2 enhancer region was cloned from mouse genomic DNA into KpnI and XhoI sites of the pGL3 promoter vector (E1751, Promega). MITF proximal promoter was cloned into SacI and HindIII sites of the PGL4 promoter vector (E6651 ...
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bioRxiv - Molecular Biology 2020Quote: ... we isolated the sequence-paired sites from the native mouse and human Hes1 and Hes5 genes and cloned these fragments into the promoterless pGL3-Basic vector (Promega). Our synthetic promoters ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were then incubated in species specific secondary HRP-conjugated antibody (anti-rabbit, Promega, W4011 and anti-mouse, Promega, W4021) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were then incubated in species specific secondary HRP-conjugated antibody (anti-rabbit, Promega, W4011 and anti-mouse, Promega, W4021) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the cDNA fragments corresponding to the coding region or 3′-UTR of mouse Inka2 cDNA were subcloned into a pGEM-3Z plasmid vector (Promega) for in vitro transcription ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed in 1×PBST and then incubated with the secondary antibody (anti-rabbit or anti-mouse IgG conjugated with horse radish peroxidase at 1:10 000, Promega). The membranes were then revealed using an ECL kit (GE Healthcare ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies were revealed using species-appropriate HRP-conjugated secondary antibodies (anti-rabbit or anti-mouse IgG (1:5000, Promega), or anti-guinea pig IgG (1:5000 ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from NMRI mouse cortical neuron cultures at DIV1 and DIV20 using the Reliaprep RNA Miniprep system (Promega) followed by deoxyribonuclease I (NEB ...
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bioRxiv - Genomics 2023Quote: Promoters were amplified from mouse genomic DNA using primers listed in table S1 and cloned into pGL3-Basic (E1751, Promega) using SacI and BglII.
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively.
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bioRxiv - Cell Biology 2024Quote: ... the Deaf1 promoter region was first PCR-amplified from genomic DNAs of C2C12 mouse myoblasts and cloned into the pGL4.20 vector (Promega, E675A). ...
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bioRxiv - Cell Biology 2019Quote: ... then alkylation solution was removed and gel pieces were hydrated for 45 min at 4 °C in digestion solution (5 ng/μl trypsin, sequencing grade, Promega, in 25 mM AB). The trypsin digestion proceeded for 2 hours at 37 °C on Thermomixer (750 rpm ...
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bioRxiv - Cell Biology 2019Quote: ... then alkylation solution was removed and gel pieces were hydrated for 45 min at 4°C in digestion solution (5 ng/μl trypsin, sequencing grade, Promega, in 25 mM AB). The trypsin digestion proceeded for 2 hours at 37°C on Thermomixer (750 rpm ...
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bioRxiv - Immunology 2020Quote: ... After a migration time of 4 h at 37°C (5 % CO2) lower compartment were analyzed for cell content by Cell-Titer-Glo® assay (Promega, Madison, WI, USA). Percentages of migrated cells were calculated as described before45 and normalized to the CXCL12-only control.
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bioRxiv - Plant Biology 2023Quote: ... The PHN-SpCas9-4×BsaI was then digested with BsaI and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). The annealed oligos FaPDSF1/R1 and FaPDSF2/R2 were mixed and ligated to the purified pHN-SpCas9-4×BsaI to form the final pHN-SpCAS9-FaPDSsgRNAs construct.