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Citations for Promega :
701 - 750 of 2251 citations for Gem Associated Protein 6 GEMIN6 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were cultured in DMEM+10% FBS+Penicillin/Streptomycin and transfected using FuGENE 6 (Promega). Co-recruitment assays were performed 24 hours after transfection ...
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bioRxiv - Immunology 2020Quote: All VLPs were produced by transient transfection of HEK293T cells with Fugene 6 (Promega, ref E2691). HEK293T were seeded in 15-cm dishes to reach 60-70% confluency the next day and VLPs were produced by co-transfecting plasmids encoding Gag-eGFP and the VSV-G envelope (pGag-EGFP and pCMV-VSV-G ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: Three wells of a 6-well plate were lysed were lysed in Reporter lysis buffer (Promega) for 10 min on ice before centrifugation at 17,000 g ...
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bioRxiv - Microbiology 2021Quote: All virus stocks were produced by plasmid transfection of HEK 293T cells with Fugene 6 (Promega). Supernatants were harvested at 48h and 72h ...
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bioRxiv - Cell Biology 2022Quote: ... and pCMV-hyPBase was performed using 500 ng each DNA and 6 μl of FugeneHD (Promega) as per manufacturer’s instructions20,49,50 ...
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bioRxiv - Biochemistry 2022Quote: ... Sf21 insect cells were transfected with PLP/DM20 bacmid using Fugene 6 transfection reagent (Promega Corp.) and baculoviruses were collected and used for preparation of a high-titer virus stock ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were examined for viability every 4 to 6 weeks with the CellTiter-Glo assay (Promega). Cell viability was measured in quadruplicates by seeding the cells (2,000 to 3,000 per well in 96-well plate) ...
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was combined at a 3:1 ratio of FuGENE® 6 Transfection Reagent (Promega) to DNA and incubated 20 min at RT ...
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bioRxiv - Molecular Biology 2022Quote: Lentivirus particles were collected from HEK293T cell supernatant 3 days after co-transfection (FuGENE 6, Promega) of lentiviral plasmid constructs (Supplementary Table 2A ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To transform the bacmid into Sf9 cells the transfection reagent FuGene 6 (Promega Corporation, Madison, USA) was used according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... HEK 293T cells were transfected with the pseudovirus encoding plasmids using FuGENE 6 Transfection Reagent (Promega). The virus culture supernatant was harvested at 48h and 72h post transfection and stored at -80°C until use ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell viability was assessed at day 6 post inhibitor treatment using CellTiter Glo 3D (Promega) according to manufacturer’s instruction.
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bioRxiv - Cell Biology 2022Quote: Lipofectamine 3000 or FuGENE 6 transfection reagents (ThermoFisher Scientific/Invitrogen, cat# L3000008; and Promega, cat# E2691) were used for transient delivery of PRR14 plasmids ...
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bioRxiv - Genetics 2023Quote: ... Transient expression of NSD2 VUS in HeLa cells was performed using FuGENE 6 (Promega, WI, USA). pCMV-3xFLAG-NSD2 and pCMV-3xFLAG-NSD2 c.2714C>T plasmids were constructed by VectorBuilder (IL ...
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bioRxiv - Biophysics 2023Quote: We stably transfected U2OS cells with this plasmid using Fugene 6 following the manufacturer’s instruction (Promega). α-Amanitin (SIGMA #A2263 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5μg of plasmid was transfected into 6-well dishes using Fugene HD (Promega, Madison, WI, USA) with 3:1 transfection reagent to DNA for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells plated for transfection were allowed to reach 70% confluency and transfected with FuGene 6 (Promega) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... then transfected with 4 μg pLZRS-GCaMP6 DNA plus 12 μl FuGENE 6 (Promega Cat. #E2691) in 800 μl of Opti-MEM (Thermo-Fisher Cat ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells were transfected using 2 µg of plasmid DNA and 6 µL of FuGENE (Promega) for gene editing or using a NEPA21 electroporation system (Nepa Gene ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transiently transfected with 1.5 μg of plasmid using FuGENE 6 transfection reagent (#E2692, Promega), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... After washing with TBST and incubation for 1 hour with anti-Rabbit IgG HRP Conjugate (Promega, 1:2,000) or anti-Mouse IgG HRP Conjugate (Promega ...
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bioRxiv - Physiology 2022Quote: ... 1:1000 mouse anti-ATP1A1 followed by 1:2500 goat anti-mouse HRP-conjugated secondary (catalog #: W4021; Promega). p-ATP1A1Y260 and ATP1A1 bands were visualized with SuperSignal Pico utilizing the ChemiDoc.
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bioRxiv - Immunology 2020Quote: ... the membrane was incubated with HRP-conjugated goat anti-rabbit IgG diluted 1:2000 (Promega, Madison, Wisconsin, USA) for 2 h at RT ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... membranes were reblotted to detect β-actin expression using primary (Cell Signal #3700) and secondary-HRP (Promega #W402B) antibodies and developed as described.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with trypsin (Trypsin Gold, Promega) overnight at 37 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... proteins were in-gel digested with trypsin (Promega) and the resulting peptide mixtures were processed on STAGE tips (92 ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL of Protein Precipitation Solution (Promega, Wisconsin) was added ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested overnight with trypsin (Promega) at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... the proteins were digested with trypsin (Promega, UK) at an enzyme-to-substrate ratio of 1:100 ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were digested with sequencing-grade trypsin (Promega) and analyzed by nano LCMS/MS as previously described (Chicher et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko 37 ...
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bioRxiv - Microbiology 2021Quote: ... Digested protein suspensions with 4 μg trypsin (Promega) in 40 μL 25 mM NH4HCO3 buffer at 37 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... with that of the Halo-tag protein (Promega), with the insertion of a 45-base linker (15 amino acids ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko49 ...
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bioRxiv - Microbiology 2020Quote: ... to enable visualization of protein bands (Promega, USA). Each experiment was performed at least three times.
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bioRxiv - Microbiology 2020Quote: Proteins were digested with sequencing-grade trypsin (Promega) and analyzed by nanoLC-MS/MS on a TripleTOF 5600 mass spectrometer (Sciex ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested with trypsin (Promega, Madison, WI) at an enzyme-to-substrate ratio of ~1:50 for 12 hours in a thermomixer ...
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bioRxiv - Microbiology 2020Quote: ... the proteins were proteolytically digested with trypsin (Promega) and purified using OMIX C18 Mini-Bed tips (Agilent Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... total proteins were extracted in lysis buffer (Promega), then Firefly and control Renilla LUC activities were quantified with FLUOstar Omega luminometer (BMG Labtech ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested using trypsin (Trypsin Gold, Promega) in a 1:50 protein to enzyme ratio and incubation for 18h at 37°C on a thermo-shaker at 600 rpm ...
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bioRxiv - Developmental Biology 2021Quote: ... proteins were trypsinized (Sequencing Grade Modified Trypsin, Promega) and digested peptides were collected by centrifugation ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were further digested with trypsin (Promega) in 10 ng/µL (enzyme-to-protein ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were then digested with modified trypsin (Promega) at an enzyme/substrate ratio of 1:50 in 100 mM ammonium bicarbonate ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were then digested with modified trypsin (Promega) at an enzyme/substrate ratio of 1:50 in 100mM ammonium acetate ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were digested with trypsin (Promega, Fitchburg, WI) for 14 hours at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko [46] ...
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bioRxiv - Neuroscience 2020Quote: ... Protein A/G magnetic beads (100 µL) (Promega) were washed with HB prior to addition to the RiboTag-IP fraction and were rotated at 4°C overnight ...
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bioRxiv - Immunology 2020Quote: ... protein samples were treated with PNGase F (Promega) according to manufacturer’s instruction under denaturing conditions ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested with trypsin (Promega, Madison, USA) at an enzyme-to-protein ratio of 1:100 (w/w ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were digested with sequencing-grade trypsin (Promega) at room temperature while shaking overnight ...