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Citations for Promega :
701 - 750 of 2848 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... according to the instructions with the following modifications: stock oligo(dT)15 primers (C1101, Promega) concentration was 50 ng/μl and 0.5 μl Superscript II enzyme was used per reaction ...
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bioRxiv - Genomics 2024Quote: ... and lysed in 65 μL 1X passive lysis buffer (in PBS) for 15 min (Promega). 20 μL lysate was then transferred to an opaque flat-bottomed plate for reading with a luminometer (Veritas) ...
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bioRxiv - Cell Biology 2024Quote: ... and samples were digested with 1:15 mass ratio of Sequencing Grade Modified Trypsin (Promega) using an S-trap mini device (Protifi ...
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bioRxiv - Biochemistry 2024Quote: ... Next, luciferase substrate (Furimazine, Nano-Glo® substrate (Promega, #N1110, final concentration of 15 μM)) was added and luminescence was measured using a PheraSTAR plate reader (BMG ...
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bioRxiv - Cell Biology 2024Quote: ... using 15 µL of 7 ng/µL of modified porcine trypsin (Promega, Madison, WI, USA) in 25 mM NH4HCO3 ...
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bioRxiv - Genetics 2024Quote: ... according to manufacturer’s protocol and subjected to two 15-minute rounds of DNase I (Promega) treatment ...
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bioRxiv - Developmental Biology 2022Quote: ... Dual-luciferase assays were performed using the Promega Dual-Luciferase Reporter (DLR™) Assay Systems with the Promega GloMax 20/20 Luminometry System (Promega, Madision, WI) (Stepicheva et al. ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mM iodoacetamide (IAA) and proteins were eluted by digestion with 5 µg/ml trypsin (Promega). Eluted proteins were fully digested overnight ...
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bioRxiv - Plant Biology 2021Quote: ... 5 units GoTaq DNA polymerase (Promega) and water to 50μl ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL ADP-Glo reagent (Promega) was added to each reaction mixture and incubated for 40 min at 25°C ...
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bioRxiv - Biochemistry 2020Quote: ... and 5 µL RNasin Plus (Promega) per sample to 800 µL lysate and 1.5-2 hours of rotation at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μM HaloTag-TMR ligand (Promega), Ready-Lyse Lysozyme (47 U/μl ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of CTG reagent (Promega G7571 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μl of CellTiter-Blue (Promega) was added and cells were incubated for 90 minutes at 37°C before measuring fluorescence using an EnVision plate reader (PerkinElmer) ...
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bioRxiv - Neuroscience 2023Quote: ... 5 mg/mL proteinase K (Promega)) and incubated overnight at 50°C ...
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bioRxiv - Immunology 2023Quote: ... 5 ng of pRL-TK (Promega), and 5 ng of pEF-Slc46 expression plasmid using GeneJuice (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... The phRL-TK (5 ng) (Promega), expressing the Renilla luciferase gene under the control of the HSV-TK promoter ...
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bioRxiv - Immunology 2024Quote: ... 0.25 µL of 5% digitonin (Promega); 8.75 µL of nuclease-free water ...
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bioRxiv - Cell Biology 2024Quote: ... 5 ng/ul Trypsin Gold (Promega). Incubation lasted 30 minutes at 30 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we dissolved 10 μg of protein in 10 μl 10 M urea containing 0.1% ProteasMax (Promega. Madison, WI, USA). Cysteine residues were reduced with 5 mM DTT (30 min at 50 °C ...
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bioRxiv - Molecular Biology 2021Quote: After 24 hours a luciferase assay was performed according to the instructions of the Dual-Luciferase Reporter Assay System kit (Promega) or the cells were resuspended in TRI Reagent (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed 24 h after transfection and luciferase activities were measured with the Dual-Luciferase Reporter Assay System (Promega) in a GloMax 20/20 luminometer (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were lysed 24 h after chemical exposure to measure luciferase activity (Firefly luciferase assay system; Promega Madison WI, USA) and β-galactosidase activity ...
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bioRxiv - Cell Biology 2021Quote: ... Luciferase activity was tested 24 hours after plasmid transfections using the Dual-Glo Luciferase Assay System kit (Cat# E2920, Promega) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Cell death was measured 24 hours post seeding via luminescence using the Caspase Glo assay (Promega, Madison, WI, USA: #G8090). Annexin-V /7AAD staining (Biolegend ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were harvested 24 hours post-transfection and RLuc and FLuc activities were measured by Dual Glo luciferase assay (Promega), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: Epithelial cytotoxicity was determined by quantification of lactate dehydrogenase activity in A549 culture supernatants at 24 h post fungal challenge using the Cytox 96 Non-Radioactive Cytotoxicity Assay kit (Promega) according to the manufacturer’s protocol and using recombinant porcine LDH enzyme (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... T cells was detected by co-culturing with MDA-MB-231 GFP-Luc and Hep3B GFP-Luc at various E/T ratios for 24 hours or 96 hours followed by measurement of the luciferase activity using the luciferase assay system (Promega) on Victor (PerkinElmer) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were treated with the indicated concentration of CHIR99021 24 hours after transfection and after another 24 hours medium was removed and the cells were harvested with 50 µl Passive Lysis Buffer (E1941, Promega). Luciferase activity was measured on a GloMax Navigator (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... RPE1 wild type cells were transfected with PX459 plasmid containing the corresponding guide sequence for 24 hours using FuGENE 6 (Promega). Following 72 hours of 4 μM Puromycin selection ...
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bioRxiv - Immunology 2022Quote: ... were transfected in keratinocytes (24 wells, cultured with 400 µl KGM2) using the transfection reagent Fugene HD (Promega, Madison, WI). 24 h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... Media was changed 24 hours post infection and luciferase levels were measured 48 hours post infection using Steady-Glo Luciferase Assay system (Promega) per the manufacturer’s recommendation and an automated plate reader ...
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bioRxiv - Microbiology 2020Quote: ... Cells were harvested after another 24 hours and firefly luciferase and Renilla luciferase expression were determined using the Dual-Luciferase Assay Kit (Promega).
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bioRxiv - Microbiology 2022Quote: 293T cells were co-transfected with pEGFP-N1 and plasmids expressing SARS-CoV-2 Spike (FL) or SARS-CoV-2 Spike-Δ19 for 24 h using FugeneHD (Promega). The cells were treated with DMSO or 10 µM 6-TG at 4 h post- transfection ...
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bioRxiv - Immunology 2022Quote: A total of 200,000 RAW cells were seeded on a 24-well plate and were co-transfected with NFATc1(pGL3-NFAT) and Renilla luciferase plasmid (pRL-TK, Promega) in a ratio of (2:1) ...
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bioRxiv - Biochemistry 2020Quote: ... PCR products were resolved on a 24% TBE gel and visualized using ethidium bromide or cloned into pGEM-T (Promega) and Sanger sequenced ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed 24 hours post-transfection with 1x Passive Lysis and reporter activities measured using a dual luciferase kit (Promega).
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bioRxiv - Microbiology 2021Quote: ... and 24 hours later cells were lysed with Passive Lysis 1x and reporter activities measured with Dual Luciferase Reporter Kit (Promega). At the same time supernatants from p1 cells were cleared by centrifugation and passaged onto fresh HEK293T-TIM1 p2 target cells ...
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bioRxiv - Biophysics 2021Quote: ... were plated into six-well tissue culture plates containing collagen-coated glass cover slips 12 to 24 h before being transfected with 1 μg of the indicated plasmid DNA using FuGENE 6 transfection reagent (Promega) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Cells were immediately stimulated with cytokine for 24 hr and nanoluciferase activity was measured relative to control activity using the NanoGlo Dual Luciferase kit (Promega). The primers for cloning enhancer fragments into pNL3.1 are in Table S3.
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bioRxiv - Biochemistry 2022Quote: ... Luciferase activity was determined 18-24 hr post-transfection from cell lysate using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturers protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis was measured by detecting Caspase 3/7 activity after 24 hours of treatment using the Caspase-Glo 3/7 Assay System (Promega) on a BMG CLARIOstar plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed after exactly 24 hours of stimulation in 1X Passive Lysis Buffer (100 μl per well (Promega, #E1941)) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated on three 10 cm tissue culture dishes per condition and transfected 24 h before lysis using FuGENE 6 (6-12 μg total plasmid DNA; Promega). Published protocol can be found on Protocols.io (dx.doi.org/10.17504/protocols.io.kxygx3zeog8j/v1).
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bioRxiv - Microbiology 2023Quote: ... Cell proliferation was assessed every 24 h by the MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation, Promega). DMSO-treated cells were used as negative controls and the effects on cell proliferation were expressed as the percentage inhibition compared to the cells of the control group ...
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bioRxiv - Biochemistry 2023Quote: ... 6 or 24 hours as indicated in the respective figure legends before lysis using the HiBiT lytic assay buffer (Promega) per manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were harvested 24 h of post-transfection and RLuc and FLuc activities were measured by Dual Glo luciferase assay (Promega), according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... The cell lines were authenticated at source and tested randomly at regular intervals for tissue specimen provenance and cell lineage at the AU Center for Pharmacogenomics and Single-Cell Omics (AUPharmGx) using GenePrint 24 System (Promega). All cell lines are mycoplasma negative ...
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bioRxiv - Microbiology 2024Quote: ... Lactic acid production was assessed for each representative strain after 24 hours incubation in the same conditions as for the growth assessment using the Lactate-Glo Assay (Promega) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2024Quote: ... Cell viability was measured at 24 h post-irradiation using CellTiter-Glo according to the manufacturer’s instructions (Promega, Cat.# G7571). Results were expressed as relative cell viability (% ...