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Citations for Promega :
701 - 750 of 3386 citations for Alpha 1 6 Fucosyltransferase FUT8 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: XAB2-GFP and RNAP2-GFP stably expressing cell lines were produced by transfecting XAB2-GFP or RNAP2-GFP using FuGENE® 6 Transfection Reagent (Promega) according to the manufacturer’ protocol ...
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bioRxiv - Immunology 2021Quote: Pseudoviruses expressing complete SARS-CoV2 spike genes were prepared by transient transfection of HEK293T cells with three plasmids: SARS-CoV2 MLV-gag/pol and MLV-CMV-luciferase plasmids using Fugene 6 (Promega Inc.) as described earlier (Rogers et al. ...
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bioRxiv - Microbiology 2022Quote: ... The effector cells and serum were incubated with the cells for 6 hours after which 75ul Bio-Glo reagent (Promega; G7940) was added to each well ...
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bioRxiv - Physiology 2024Quote: ... a targeting vector was constructed by subcloning a 9.1 kb region from an Akr1b7 positively identified C57BL/6 fosmid clone (WI1-1577M19) into the 2.45 kb-iTL cloning vector derived from the pSP72 vector (Promega; Madison, WI). The region was designed so the long homology arm (LA ...
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bioRxiv - Microbiology 2024Quote: ... and simultaneously transfected with 0.4 μg of respective EGFP-Rab plasmids using FuGENE 6 transfection reagent (Cat. E269A, Promega, Madison, WI) per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and gene expression was determined by quantitative PCR (QuantStudio 6 Pro Real-Time PCR System) with GoTag qPCR System (Promega, A6002). Actb and Rplp0 were used as housekeeping genes ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with 12 µg of plasmid expressing the spike from various variants using Fugene-6 (Promega; catalog number: E2691) according to the manufacturer’s instructions and incubated at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... transfection was carried out by adding 2 μg of freshly prepared bacmid DNA and 6 μL of FuGene HD transfection reagent (Promega, E2311), following the instructions provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... or pCEP4-OCT4-T234A-S235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: ... and transfected with 1 μg of “driver” plasmid (pTMO2F3, p5UThORF2 or other “driver”) + 3 μg of “reporter” plasmid (pAluneoTet, or other “reporters”) using 12 μL FuGENE® 6 (Promega); the following day ...
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bioRxiv - Molecular Biology 2024Quote: ... approximately 1×106 cells were plated and transfected with 1 μg of “driver” plasmid (pTMO2F3, p5UThORF2 or other) + 3 μg of “reporter” plasmid (pJBM560) using 12 μL FuGENE® 6 (Promega). Transfection efficiency determination ...
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bioRxiv - Microbiology 2024Quote: ... 4 μg of each of the 3 HIV-envelope expression plasmids were co-transfected with 8 μg of backbone plasmid pSG3ΔEnv in HEK 293T cells using FuGene 6 transfection reagent (Promega, catalog # E5911). Virus was harvested 48 h post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Apoptosis was determined as Caspase-3/7 activity immediately after 6-day culture using Caspase-Glo 3/7 Assay (Promega, USA). Luminescence was recorded with the Infinite M200 PRO microplate reader (Tecan ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from 10 whole fly bodies collected on dry ice between ZT 6 and 8 using SV RNA Total Isolation kit (Promega #Z3101) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 6 or 20 hours and luminescence was measured following addition of luciferase substrate using luminometer (GloMax Navigator System, Promega, Madison WI).
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bioRxiv - Immunology 2024Quote: SARS-CoV-2 variant spike pseudotyped lentiviral particles were produced in 293T cells (ATCC) using Fugene transfection reagent 6 (Promega; E2691). Two million cells were seeded in D10 (DMEM(Life Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were collected 4- and 6-hours post-transfection (hpt) for Luciferase measurement using Dual-Luciferase® Reporter Assay System (Promega) and RNA extraction for RT-qPCR assay using Luna® Universal One-Step RT-qPCR (New England Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: ... and Lumit anti-rabbit antibody-SmBiT (Promega). Final concentration of each antibody is 0.15 μg ml−1 ...
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bioRxiv - Microbiology 2020Quote: ... anti-mouse IgG HRP conjugate antibody (Promega), were both used at a 1:10,000 dilutions ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by peroxidase-conjugated secondary antibodies (Promega). Signal was developed using ECL substrate (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2022Quote: ... and secondary antibodies (W401B and W402B, Promega). ChIP was performed with anti-TAP and protein A Dynabeads (CAB1001 and 10001D ...
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bioRxiv - Cell Biology 2022Quote: ... anti-Halo (ProMega; #G9211; monoclonal mouse antibody) anti-SNAP (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... and developed using AP-coupled antibodies (Promega) and NBT/BCIP as substrates (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... the G9281 rabbit anti-HaloTag antibody (Promega) was used at a dilution in the range of 1:30 to 1:500 (1:500 is the working dilution for immunofluorescence).
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bioRxiv - Molecular Biology 2024Quote: Antibodies used were: anti-LgBiT (Promega #N7100), anti-GFP (for EGFP/mTurq2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Horseradish peroxidase-coupled antibodies were from Promega (anti-mouse and anti-rabbit IgG HRP conjugate ...
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bioRxiv - Cancer Biology 2024Quote: ... HiBiT antibody (N7200) was obtained from Promega and KRAS antibody (LS-C175665 ...
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bioRxiv - Cell Biology 2020Quote: ... The AIO plasmid and the template plasmid were transfected into HAP1 STG-p62 (for mScarlet-AID-NBR1) or HAP1 WT (for GFP-AID-NBR1) cells using FuGene 6 transfection reagent (Promega, cat# e2691). 48h later the cells were sorted for mScarlet fluorescence in bulk ...
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bioRxiv - Microbiology 2020Quote: ... The env gene clones together with the env-deficient HIV-1 plasmid DNA (SG3Δenv) were contransfected into HEK293T cells (ATCC, Manassas, VA, USA) using the FuGene 6 transfection reagent (Promega, Madison, WI, USA). Two days after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... (complete media) were co-transfected with our library of retinopathy variants and the Bxb1 recombinase expression vector using Fugene 6 (Promega, Madison, WI). Doxycycline (2 μg/mL ...
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bioRxiv - Bioengineering 2022Quote: ... The DNA transfection reagents were prepared by incubating 3 µg of plasmid DNA encoding the Bxb1 recombinase and 6 µL of Fugene6 reagent (Promega Cat # E2693) in 300 µL of Opti-MEM for 15 minutes and then added to the cell suspension ...
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bioRxiv - Immunology 2022Quote: ... L981F. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega, Catalog #E2692). Pseudovirions for 293T/ACE2 infection were produced by co-transfection with a lentiviral backbone (pCMV ΔR8.2 ...
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bioRxiv - Molecular Biology 2020Quote: ... (49) as a negative control (2 μg of DNA per well of 6 well plate) with FuGene transfection reagent (Promega, Madison, WI) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... The mutation was confirmed by full-length Spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega Cat#E2692) and a combination of Spike plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were packaged with the third-generation lentiviral packaging system (VSV-G, pMDLg, and pRSV-REV) and expressed in HEK293T cells via transient transfection with FuGENE 6 (Promega, Madison, WI). Virus-containing supernatants were harvested after 24 and 48 hours ...
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bioRxiv - Immunology 2021Quote: ... The mutation was confirmed by full-length spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega, Catalog #E2692). Pseudovirions for 293T/ACE2 infection were produced by co-transfection with a lentiviral backbone (pCMV ΔR8.2 ...
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bioRxiv - Immunology 2021Quote: ... All mutations were confirmed by full-length Spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega Cat#E2692) and a combination of Spike plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... 1000 and 5000 µM) or remained untreated for 6 days and viability was analyzed using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Dübendorf, Switzerland) to determine the adenosine triphosphate (ATP ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated for an additional 2 – 6 days and cell growth was quantified using CellTiter-Glo® 2.0 Reagent (Promega, Cat. #924C). For immunoblot analysis ...
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bioRxiv - Biochemistry 2022Quote: ... cells grown in 10 cm dishes in complete media were co-transfected with our library of cystic fibrosis variants and the Bxb1 recombinase expression vectors using Fugene 6 (Promega, Madison, WI). Doxycycline (2 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... 6 μg of each vector was transfected into tumor cells plated on a 6-well plate using FuGENE6 transfection reagent (Promega, cat. E2691) and OPTI-MEM (GIBCO ...
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bioRxiv - Physiology 2023Quote: ... UMR106 cells were plated at 4 × 105 per well in a 6-well plate and transfected with 2 µg of NFAT5 gRNAs per well using the FuGENE® HD Transfection Reagent (Promega, USA). 24 h post-transfection ...
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bioRxiv - Genetics 2023Quote: ... 3,500,000 cells were seeded in 10 cm plates (2-4 per replicate) and transfected with FuGENE® 6 Transfection Reagent (Promega, E2692). In one tube ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from the cells of 6-well plates treated as above using the Maxwell RSC simplyRNA kit and the Maxwell RSC Instrument (both Promega, Southampton, UK) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and 5 µg of the pHR’CMV-Luc (luciferase reporter) plasmid in 10-cm dishes containing 50-80% confluent 293T cells in 10% FBS/DMEM using Fugene 6 (Promega, Madison, WI). 48 h after transfections ...
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bioRxiv - Molecular Biology 2024Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
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bioRxiv - Biophysics 2024Quote: ... For PMP22 experiments: 24 hrs later cells were transfected with 15 ug of pSF PMP22 N41Q-myc using Fugene 6 (Promega cat # E2691) following the manufacturers protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The extracted plasmid was transfected with packing plasmid (psPAX2) and envelope VSV-G plasmid (pMD2.G) using FuGENE® 6 Transfection Reagent (Promega, E2691). The following lentivirus harvest ...
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bioRxiv - Genetics 2024Quote: ... and incubated with primary antibodies in a blocking buffer (1ξ PBS, 0.5% BSA) and then with alkaline phosphatase-conjugated secondary antibody (Promega). The color reaction was developed with NBT/BCIP solution (Nacalai Tesque).
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bioRxiv - Developmental Biology 2024Quote: ... The membranes were washed 3x 10 minutes in TBS-T before being incubated for 2 hours with the secondary anti-mouse-HRP- or anti-rabbit HRP antibody (1:5000, Promega anti-mouse, W4021; anti-rabbit W4011). The membranes were washed in TBS-T for 3x 10 minutes and developed with Western Lightning Plus-ECL reagent (Perkin Elmer NEL104) ...