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Citations for Promega :
701 - 750 of 1491 citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Cell death after 5 h treatment was quantified by PI incorporation or using the Caspase Glo system (Promega) according to manufacturer instructions.
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bioRxiv - Immunology 2021Quote: ... and HHEX (NM_002729) attached to a FLAG tag at the 5’ end and HiBit tag (Promega, Madison, WI) at the 3’ end ...
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bioRxiv - Cell Biology 2021Quote: The Rosa26 promoter and the 5’UTRs of secretory granule protein transcripts were subcloned into the pNL1.1 (Promega) using standard cloning techniques ...
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bioRxiv - Microbiology 2022Quote: ... reverse transcription was performed with 0.5 μl (corresponding to 5 U) of Avian Myeloblastosis Virus Reverse Transcriptase (Promega), 0.1 μl dNTP mix (10 mM) ...
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bioRxiv - Biophysics 2022Quote: ... The poly-L-lysine coated side of the cover slip was exposed to 5 mg/ml streptavidin (Promega) in this space for 15 min ...
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bioRxiv - Microbiology 2022Quote: ... Then reaction was stopped by the addition of 5.5 μL of a mix containing 5 % SDS and 0.25 M EDTA and deproteinized with proteinase K (Promega) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were then concentrated down to 20 µL and supplemented with 5 µg of sequencing grade trypsin (Promega) in 15% ACN in 200 mM aqueous TEAB ...
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bioRxiv - Molecular Biology 2023Quote: ... 1M Tris-HCl was added to adjust the pH and 5 μL trypsin (2.5 ug, Promega Sequence Grade) was added and the samples digested overnight at 37℃ ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was assessed 5 days after interferon-β treatment with CellTiter-Glo Luminescent Cell Viability Assay (Promega). ATP bioluminescence values of the interferon-β treated wells were normalized to those of the vehicle-treated controls ...
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bioRxiv - Neuroscience 2023Quote: ... DA at concentrations ranging from 0.01 nM to 100 μM and 5 μM furimazine (NanoLuc Luciferase Assay, Promega) were then bath-applied to the cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Subsequent measurements were taken every 24 hrs for 5 days using CellTiterGlo Luminescent Cell Viability Assay kit (Promega) and SpectraMax plate reader (Molecular Devices ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μL culture were added to 45 μL NanoGlo reagent (1x Promega lysis buffer and NanoGlo reagent; Promega). The average luminescence of the background cultures was subtracted to the average luminescence of each sample.
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bioRxiv - Neuroscience 2024Quote: ... The cells were then transferred to opaque 96-well plates containing 5 μM furimazine (NanoLuc Luciferase Assay, Promega) and bathed in ACh at concentrations ranging from 0.1 nM to 100 μM ...
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bioRxiv - Microbiology 2024Quote: ... Ten nanograms of template DNA was added to 5 µL Green GoTaq Reaction Mix Buffer (Promega, Madison, WI), 1.25 units of GoTaq DNA Polymerase (Promega ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription reactions were performed by adding 0.5 µl (corresponding to 5 U) of AMV Reverse Transcriptase (Promega), 0.1 µl dNTP mix (10 mM) ...
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bioRxiv - Cancer Biology 2024Quote: ... lactate assay was performed using 5 µl of media and the luminometric Lactate-Glo assay kit (Promega, #J5021) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μL of a mixture of 10 mM dNTPs and 200 U of M-MLV reverse-transcriptase (Promega) were added to the mixture and incubated at 37 °C for 60 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... shaken vigorously and sonicated in water bath for 5 min before 50 µL of 0.2% ProteaseMAX (Promega, USA) in 10% acetonitrile (ACN ...
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bioRxiv - Genetics 2024Quote: ... Exon 5 and exon 17 of PNPT1 were amplified by PCR using GoTaq® Green Master Mix (Promega) and the primers 5a - 5b for exon 5 and 17a - 17b for exon 17 ...
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bioRxiv - Genetics 2020Quote: ... 2.4 µl of RQ1 DNase Buffer and 2 µl of RQ1 RNase-Free DNase (M6101, Promega) was added to each RNA sample and incubated for 15 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cDNA was synthesized from 2 μL of the eluted RNA using M-MLV reverse transcriptase (Promega) and random hexamer primers ...
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bioRxiv - Developmental Biology 2021Quote: ... and the resulting mRNAs (2 μg) were translated in 50 μl of rabbit reticulocyte lysate (Promega). Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Viral RNA of SARS-COV-2 was detected by TaqMan®-based Real-Time PCR (Promega) using a CDC protocol with two sets of primer/probe which amplifies virus nucleocapsid (N ...
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bioRxiv - Molecular Biology 2021Quote: ... thiopropyl sepharose beads were incubated overnight at 37°C with 2 μg trypsin Gold (Promega, #V5280) in 50 mM NH4HCO3 ...
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bioRxiv - Genetics 2021Quote: ... The purified DNA was PCR amplified using 2× GoTaq Colorless Master Mix (Promega, Madison, WI, USA), and PCR product was extracted as above to eliminate primer-dimers ...
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bioRxiv - Plant Biology 2021Quote: ... 8 µL of a mix consisting of 2 µL deoxyribonucleotide triphosphate (dNTPs, Promega, Madison, Wisconsin, US), 4 µL 5x RT-buffer ...
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bioRxiv - Plant Biology 2020Quote: ... were digested for 12 hr at 37 °C with 2 mg of sequencing-grade trypsin (Promega), followed by a second incubation for 6 hr with an additional 2 mg of trypsin ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 2 μg of total RNA with the MMLV kit (Promega, Madison, WI). Semi-quantitative PCR was performed within the linear amplification phase using a recombinant Taq Polymerase (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... 2 μg RNA was used for cDNA synthesis with M-MLV Reverse Transcriptase (Promega, Madison, USA) and 1 μL of the obtained cDNA was used for real-time PCR with SYBR™ green master mix (Bioline ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was then diluted to <2 M urea and incubated with 50 μg trypsin (Promega) overnight at 37 °C under agitation ...
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bioRxiv - Microbiology 2020Quote: ... Urea was diluted to 2 M with 100 mM Tris-HCl pH 7.0 and trypsin (Promega) was added at a mass ratio of 1:100 (trypsin:protein) ...
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bioRxiv - Neuroscience 2020Quote: ... all cells were incubated in the following: mouse anti-βIII tubulin (1:1000; 2 hr; Promega) and goat anti-mouse Alexa 546 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... wild type or mutant 3′-UTR of DKK3 was cloned into the psicheck-2 vector (Promega). HCT116 cells were transfected with and wild-type or mutant 3′-UTR-luc by using Lipofectamine 3000 ...
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bioRxiv - Microbiology 2022Quote: ... 2 μl of the gDNA were added to a GoTaq® Green Master Mix (Promega, USA) and used to amplify part of the mitochondrial cytochrome oxidase 1 (CO1 ...
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bioRxiv - Plant Biology 2021Quote: ... 2-3 μg of the purified RNA was in vitro translated using wheat germ extract (Promega) at 25 °C for 2 h as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: The SARS-CoV-2 spike pseudotyped virus activity was determined by bright-glo luciferase assay (Promega). The plate reader detected the luminescence two days post virus infection or without virus infection ...
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bioRxiv - Microbiology 2020Quote: ... JAK2 and MAP3K2 are cloned downstream of Renilla luciferase (Rluc) in the modified psiCHECK-2 (Promega) plasmid p5522 using the Gateway cloning technology (m Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... hepatica calmodulin 2 (fhcam2, AM412547) were generated using the T7 RiboMAX™ Express RNAi System (Promega) with primers listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... BT-549 and HCC38 cells were transfected with both plasmids simultaneously (2 μg) using FuGene (Promega) according to the manufacturer’s instructions ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... 30 μg of protein was taken for digestion with trypsin (2% (w/w)) (Promega, United States) and left overnight at 37°C (∼18-hours) ...
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bioRxiv - Microbiology 2020Quote: ... incubated in the dark for 2 mins and read on a Glomax Multi+ Detection System (Promega).
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bioRxiv - Microbiology 2020Quote: ... 18h (SARS-CoV-2) or 24h (SARS-CoV) later by adding 1μM of Coelenterazine-H (Promega) at 1:400 dilution in PBS ...
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bioRxiv - Systems Biology 2021Quote: ... Both 1° and 2° assays were performed with 2x PCR master mix (Promega Corporation, Wisconsin, USA). The final PCR product from the 2° nested PCR was checked by electrophoresis in a 1.5% agarose gel ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription of 2 μg RNA was performed using M-MLV reverse transcriptase (Promega, WI, USA). The PCR-based amplification was done using synthesized-cDNA ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with 2 μg DNA using FuGENE® HD Transfection Reagent (REF E2311, Promega). After 24 hours ...
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bioRxiv - Biophysics 2020Quote: ... Cytotoxicity was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay kit (Promega) by determining Formazan absorbance at 590 nm on a Tecan Safire automated plate reader ...
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bioRxiv - Microbiology 2020Quote: Supernatants were taken from cell medium and mixed with equal volume of 2 ×lysis buffer (Promega). Luciferase activity was measured with Renilla luciferase substrate (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... For RNA retrotranscription and RT-PCR it was used GoTaq 2-Step RT qPCR System (Promega) according to the manufacturer’s instructions and it was performed using Mic qPCR Cycler (BioMolecular Systems ...
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bioRxiv - Microbiology 2021Quote: Supernatants were taken from cell medium and mixed with equal volumes of 2×lysis buffer (Promega). Luciferase activity was measured with Renilla luciferase substrate (Promega ...
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bioRxiv - Immunology 2021Quote: ... Purified mAb (2 mg/mL in PBS) was treated with 1 unit of IdeS protease (Promega) per 1 µg of mAb ...