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Citations for Promega :
651 - 700 of 1604 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: 100 μg of K562 cell tryptic digest (CAT# V6951, Promega, Madison, WI, USA) was adjusted to 50 ng/μL with 50% ACN containing 0.1% TFA and dispensed in 10 μL each into normal tubes (1.5-mL safe-lock tube ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 100 µL per well Promega Nano-Glo HiBit lytic detection reagent mix (Promega Nano-Glo HiBit Lytic Detection System #N3050) ...
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in 100 mM (NH4)HCO3 (pH 8.0, 0.2% w/v Promega ProteaseMax) and sequentially treated with TCEP (5 mM ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... and ligation for 4.5 hours with 100 units T4 DNA ligase (Promega, #M1794) under diluted conditions (7 ml) ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: Proteins were digested overnight at 37°C in 20 ml of 25 mM NH4HCO3 containing sequencing-grade trypsin (12.5 mg/ml; Promega). The resulting peptides were sequentially extracted with 70% acetonitrile and 0.1% formic acid ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were resuspended in 4 ml of Blob100 buffer plus 80 units/ml RNasin Plus (Promega, Flitchburg, WI, USA) and lysed under liquid nitrogen in a 6870 Freezer Mill (Spex ...
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bioRxiv - Microbiology 2021Quote: ... Urea was reduced to 1 M by addition of 500 ml AB and overnight digestion was performed at 37°C with 5 ml of freshly prepared 0.2 mg/ml trypsin (Promega) in AB ...
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bioRxiv - Microbiology 2019Quote: ... Digestion product was cooled and transferred to a pre-chilled 15 ml tube containing 4 ml 1.1X Ligase Buffer (Promega) and 1% Triton X-100 ...
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bioRxiv - Cancer Biology 2020Quote: ... Collagenase IV (all at a concentration of 1 mg/ml) and Dispase (2mg/ml) in the presence of RNase inhibitors (RNasin (Promega) and RNase OUT (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... post-transfection with or without OSM (10 ng/mL for 8 h) or IFN-α (1,000 U/ml for 16 hours) before lysis using Passive Lysis Buffer (Promega). Firefly and Renilla luciferase activity was then determined in a dual luciferase assay ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Recombinant baculovirus was produced by transfecting recombinant bacmids (2-3 μg) into Sf9 cells (5 mL, density of 4×105 cells per mL) using FuGENE HD Transfection Reagent (Promega) and Opti-MEM Reduced Serum Media (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were lysed by the addition of 150 μl of lysis solution (1.25 mg/ml lysozyme, 2.5 mg/ml BSA, 1X CCLR) (Promega, WI, USA) and incubating at room temperature for 10 min ...
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bioRxiv - Immunology 2020Quote: ... anti-m6 A conjugated beads were incubated with purified mRNA with rotation at 4C overnight in 300 mL MeRIP buffer with 1 mL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Microbiology 2022Quote: ... A 5 mL sample was then mixed with 10 mL of RNAprotect and processed with the SV Total RNA Isolation System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... gel pieces were reswelled in 60 mL of 50 mM NH4HCO3 buffer containing 10 ng/mL of trypsin (modified porcine trypsin sequence grade, Promega) incubated for one hour at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... In the next step proteins were digested overnight using 100 ng/µl trypsin (Promega) at 37°C ...
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The key features of SARS-CoV-2 leader and NSP1 required for viral escape of NSP1-mediated repressionbioRxiv - Molecular Biology 2021Quote: ... 100 ng of Trypsin Gold and 1 μg of Trypsin sequenced grade (both Promega) were added to BioID eluates and total cell lysates ...
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bioRxiv - Bioengineering 2019Quote: ... medium was removed and 100 or 200 µl of 1x Passive Lysis Buffer (Promega) was added to 2D and 3D wells ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were lysed and 100 μL of luciferase substrate (Promega Bio-GloTM Luciferase Assay) was added into each well ...
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bioRxiv - Biochemistry 2020Quote: ... except that the efficiency of translation of 100 ng/μl firefly luciferase mRNA (Promega) in RRL was measured (in relative luminescence units ...
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bioRxiv - Systems Biology 2021Quote: ... 100-200 ng of purified digestion was ligated by T4 DNA ligase (Promega M1801), and 2 μL of the product were transferred into 40 μL of TOPO competent cells (ice incubation for 20 min ...
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bioRxiv - Microbiology 2020Quote: ... the cells were lysed in 100 μl of One-Glo Luciferase Assay Reagent (Promega). Firefly luciferase activity was determined with a Centro LB960 luminometer ...
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bioRxiv - Microbiology 2021Quote: ... 1ml of PBS with 100 μl of substrate (Nano-Glo luciferase assay system, Promega) was added per well and immediately photographed with the software option for Chemiluminescence with 40 s of exposure ...
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bioRxiv - Biochemistry 2022Quote: ... to 100 µl add 15 µl DNase I and 1.5 µl RNASin Plus (Promega) before use) ...
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bioRxiv - Biochemistry 2022Quote: 4 × 105 HEK293T cells were lysed in 100 μl of Passive Lysis Buffer (Promega). 10 μl of cell lysates were separated on a NuPAGE 4-12% Bis-Tris polyacrylamide gel (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were stained with 100 nM HaloTag Ligand JF646 (Promega, product number GA1121) and 1 μm Hoechst 33342 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and were subsequently stained with 100 nM Halo Ligand JF549 (Promega, product number GA1111) and 100 nM Halo Ligand JF646 (Promega ...
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bioRxiv - Cell Biology 2022Quote: Cells were incubated in DMEM with 100 nM TMR-conjugated Halo ligand (Promega, G8251) for 20 min ...
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bioRxiv - Cancer Biology 2022Quote: ... After 7 days of culturing 100 μl of CellTiter-Glo 3D (Promega Cat# G9681) was added to each well and incubated for 5 minutes on a shaker ...
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bioRxiv - Molecular Biology 2020Quote: ... using 100 μl of the CellTiter-Glo® Luminescent Cell Viability Assay solution (Promega), followed by luminometry using a Centro LB 960 luminometer (Berthold).
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bioRxiv - Microbiology 2019Quote: ... The cells were permeabilised by adding 20 µl of 0.2% Triton X-100 (Promega) in 1x PBS for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... 100 ng of each vector was transfected into 293T cells using Lipofectamine 2000 (Promega). Empty renilla vector was used as negative control (1ng/well) ...
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bioRxiv - Immunology 2019Quote: ... Upper transwells were removed and 100 µL of Cell Titer Glo (Promega, Madison, WI) added to each bottom well and incubated at RT for 10 min ...
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bioRxiv - Developmental Biology 2020Quote: ... 100◻mM NaCl and 0.1% Tween-20) with the NBT/BCIP substrate (Promega) to visualize the WISH signal.
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bioRxiv - Biochemistry 2019Quote: 4 x 105 HEK293T cells were lysed in 100 μl Passive Lysis Buffer (Promega). 10 μl of cell lysates were boiled with 2.5 μl 4x gel loading buffer for 5 min at 98°C and run on a NuPAGE 4-12% Bis-Tris polyacrylamide gel for 45 min at 200 V ...
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bioRxiv - Microbiology 2021Quote: ... plates were removed from the incubator and 100 μL of NanoGlo IC50 solution (Promega NanoGlo reaction mixture diluted 10-fold in PBS ...
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bioRxiv - Immunology 2020Quote: ... The supernatant was aspirated and replaced with 100 μL of Steadyglo luciferase reagent (Promega). 75 µL was then transferred to an opaque ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the addition of 100 uL Renilla Luciferase lysis buffer (Promega cat.#E2810), and appropriate incubation ...
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bioRxiv - Microbiology 2019Quote: ... Cell lysates (20μl) were mixed with 100 μl Renilla Luciferase Assay System Substrate (Promega). The luminescence level was measured and the relative light units (RLUs ...
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bioRxiv - Genomics 2021Quote: ... 100 μl of CellTiter-Glo® 3D Cell Viability Assay (Promega cat. no. G9682) were added to the wells intended for the T0 and the luminescence was recorded with Tecan Infinite® 200 Pro ...
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bioRxiv - Biochemistry 2020Quote: ... magnetic beads were prepared by taking 100 μL of Streptavidin Magnesphere Paramagnetic Particles (Promega) and incubated with 5 μM of Spike-Fc-AviTag for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... 20 μL lysate was added to 100 μL Luc substrate (Promega Luciferase Assay System). The amount of luciferase was quantified by luminescence (relative luminescence units (RLU)) ...
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bioRxiv - Neuroscience 2022Quote: ... the beads are then resuspended in 100 mM TEAB and sequencing-grade trypsin (Promega) was added to digest the biotinylated proteins on the beads into peptides ...
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bioRxiv - Microbiology 2022Quote: ... Infected cells were labeled with 100 nM Janelia Fluor 646 HaloTag ligand (Promega, GA1120) for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Five µL of Sequencing Grade Trypsin (100 ng/µl in 10 mM HCl, Promega) were added to the beads and the digestion was carried out in a microwave instrument (Discover System ...
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bioRxiv - Molecular Biology 2022Quote: ... and the proteins were digested with 1/100 (w/w) trypsin (Promega, Cat # V5111) overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Plates were stored at -20°C before adding 100 μl Steady Glo substrate (Promega) to perform a luciferase assay as described earlier ...
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bioRxiv - Systems Biology 2023Quote: ... for 4 h at 37 °C and then with trypsin (Promega, 1:100, w:w) overnight at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: The 10 to 100 bp DNA step ladder was purchased from Promega (Cat. G447A).
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bioRxiv - Microbiology 2023Quote: ... 100 μl CellTiter-Glo Reagent (CellTiter-Glo Luminescent Cell Viability Assay, Promega, cat. G7571) was added to each well for 10 min before luminescence was measured with PerkinElmer EnSight microplate reader ...