Labshake search
Citations for Promega :
651 - 700 of 751 citations for Phospholipase A and Acyltransferase 4 RARRES3 PLAAT4 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... 20 mM HEPES pH 7.4, 1mM MgCl2 with 1x c0mplete™ EDTA-free Protease Inhibitor Cocktail and 1U/μl RNasin® Plus RNase Inhibitor from Promega added right before the use) and incubating for 30 minutes on ice with pipetting every 10 minutes ...
-
bioRxiv - Cell Biology 2020Quote: ... TUNEL staining to detect apoptotic cells was completed prior to antibody stainig per manufactuors instructions (Promega, Madison, WI). TUNEL positive cells were counted and averaged from 10 images from each animal ...
-
bioRxiv - Synthetic Biology 2021Quote: ... transferred by semi-dry Western Blot onto PVDF membranes visualised with polyclonal antibody and BCIP/NBT (Promega, S3771). Anti-bodies used in this study included a monoclonal α-his-antibody (BioLegend ...
-
bioRxiv - Neuroscience 2020Quote: ... membranes were incubated for 1 hour at room temperature with an HRP-conjugated secondary antibody (1:1000, Promega). Protein expression was revealed using the Clarity kit (Biorad) ...
-
bioRxiv - Neuroscience 2020Quote: ... the following primary antibodies were used at the indicated concentrations: 1:10’000 mouse anti-β3-Tubulin (Promega, G712A), rabbit anti-p-ERK T202/Y204 (CST ...
-
bioRxiv - Neuroscience 2022Quote: ... For immunostaining of the induced neurons following antibodies were used: anti-Tuj 1 (mouse; 1:2000, G7121, Promega), anti-MAP2 (chicken ...
-
bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies were either goat anti-mouse IgG HRP or goat anti-mouse IgG HRP (Promega; 1:10,000) respectively.
-
bioRxiv - Immunology 2022Quote: The ADCC activity of anti-SARS-CoV2 S IgG antibodies was determined using the ADCC Reporter Bioassay (Promega) as previously described (Dufloo et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with PBST and then anti-mouse IgG (H&L) alkaline phosphatase-conjugated secondary antibody (Promega) was incubated with the membrane at a 1:5,000 dilution for 1 h at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein bands were detected with horseradish peroxidase (HRP)-conjugated rabbit or mouse secondary antibody [1:1,000 dilution] (Promega). The chemiluminescence signal was imaged using an Odyssey XF Imager (LI-COR ...
-
bioRxiv - Cancer Biology 2023Quote: ... followed by addition of 25 uL/well of Antibody Dependent Cellular Cytotoxicity (ADCC) Bioreporter Cells (Promega cat# G7010). The ADCC Bioreporter cells are engineered Jurkat cells stably expressing FcγRIIIa V158 (high affinity ...
-
bioRxiv - Microbiology 2023Quote: ... and then incubated with anti-rabbit horseradish peroxidase (HRP) conjugate antibodies (1:20000 dilution) (Promega, Madison, WI, USA) for two hours at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... The secondary antibodies used in this study were 1:2,500-diluted HRP-conjugated anti-mouse IgG (Promega, #W4021) and anti-rabbit IgG (Promega ...
-
bioRxiv - Molecular Biology 2024Quote: ... an extra 1-hour room temperature incubation in 1:2500 anti-Mouse IgG-HRP secondary antibody (Promega, W4021) + 1% BSA + TBS was performed prior to Clarity Western ECL detection.
-
bioRxiv - Bioengineering 2024Quote: The primary antibodies used in this study were the following: rb-α-Nanoluc (non-commercial, Promega, 1:1000); rb-α-CD63 (ab68418 ...
-
bioRxiv - Cancer Biology 2024Quote: ... following incubation with secondary antibodies and subjected to fluorescence-based TUNEL staining following the manufacturer’s protocols (Promega, G3250). Briefly ...
-
bioRxiv - Cell Biology 2024Quote: ... HRP conjugated anti-rabbit (W401B; WB: 1:10,000) and anti-mouse (W402B; WB: 1:10,000) secondary antibodies were from Promega. Goat anti-Mouse IgG (H + L ...
-
bioRxiv - Cell Biology 2021Quote: ... membranes were subjected to three wash periods for 5 minutes in 1x TBS with 0.5% Tween and then probed with horseradish peroxidase–conjugated goat anti-mouse or goat anti-rabbit secondary antibodies at 1:2,500 (W4021, W4011, Promega) in 1x TBS with 5% nonfat dry milk and 0.5% Tween at room temperature for 1 hour ...
-
bioRxiv - Plant Biology 2020Quote: ... and incubated with an anti-rabbit immunoglobulin horseradish peroxidase-conjugated secondary antibody (1:4000 dilution, lot no. 187662; Promega) to detect SOG1-Myc ...
-
bioRxiv - Microbiology 2021Quote: ... 0.1 % Tween 20 and incubated for 1 h with the secondary IgG (HRP-conjugated donkey anti-rabbit antibodies, Promega) diluted 1:20,000 in TBS ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were then incubated for 1h at room temperature with horseradish peroxidase-conjugated anti-mouse IgG secondary antibody (Promega) at 1:10,000 and peroxidase was detected using the Pierce chemiluminescence system (Pierce ...
-
bioRxiv - Plant Biology 2020Quote: ... the membrane was incubated for 1 h with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted in PBS-T with 3% milk powder ...
-
bioRxiv - Cell Biology 2021Quote: ... Supersignal kit (Pierce) and a LAS-3000 imager (Fujifilm) or AP-coupled antibodies and NBT/BCIP as substrates (Promega). Rabbit polyclonal antibodies obtained and purified using full-length Arpin were previously described (Dang et al ...
-
bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated for 1 h with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted in PBS-T with 3% milk powder ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the primary antibodies (1D4 1:1500, Anti-Calnexin Sigma c4731 1:600, Anti-LgBiT Promega N7100 1:100 ...
-
bioRxiv - Cell Biology 2023Quote: ... Secondary goat anti-mouse and anti-rabbit antibodies conjugated with alkaline phosphatase used for Western blots were from Promega.
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 3 times for 10 min in TBS-T and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10,000; Promega) in 5% non-fat dry milk -TBS-T for 1 h at room temp ...
-
bioRxiv - Microbiology 2023Quote: The potential for M2e-specific antibodies to induce ADCC was evaluated using a mouse FcγRIV ADCC Reporter kit (Promega). In brief ...
-
bioRxiv - Cell Biology 2023Quote: ... chemiluminescent substrate or alkaline phosphatase (AP)-conjugated secondary antibody and Western Blue® Stabilized Substrate for Alkaline Phosphatase (Promega). Membranes were imaged using a ChemiDoc MP imaging system (BioRad) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The milk solution was removed and the membrane was incubated with a 1:1,000 dilution of streptavidin-alkaline phosphatase antibody (Promega) in TBS-T for 1 h at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was a horseradish peroxidase polyclonal goat anti-mouse (from 1:5,000 to 1:10,000, depending on the primary antibody; Promega, W4021). Proteins were detected with the ECL chemiluminescence reagent (GE Healthcare ...
-
bioRxiv - Molecular Biology 2024Quote: ... The membrane was washed with Tris-buffered saline with 0.1% Tween-20 (TBST) and incubated with a secondary antibody (Gpx4 1:10000 or 4HNE 1:10000, W4011, Promega and Gapdh 1:10000 or ActB 1:5000 ...
-
bioRxiv - Genetics 2024Quote: ... Membranes were probed with a 1:2,000 dilution of anti Phospho-Ser23/24 TnI antibody (Cell Signalling) and 1:6,000 diluting of anti- rabbit-HRP secondary (Promega). Loading controls following antibody stripping with Restore Plus reagent (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins separated on the second gel were transferred to a PVDF membrane and DivJ levels were measured using Western blots by probing the protein with anti-HaloTag antibody (Promega). Due to the requirement of growing cells in low phosphate media ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Proteins were transferred by semi-dry Western Blot onto PVDF membranes visualised with polyclonal antibody and BCIP/NBT (Promega, S3771).
-
bioRxiv - Plant Biology 2021Quote: ... antibodies before probing with horseradish peroxidase (HRP)-conjugated anti-rabbit (for H3) or anti-mouse (for anti-GFP and anti-TUB) as the secondary antibody (1:5000; Promega, Madison ...
-
bioRxiv - Cell Biology 2020Quote: ... DRG cultures were incubated with an antibody directed against the neuronal-specific marker βIII tubulin (Promega, Madison, WI, United States) diluted 1:2000 in blocking solution ...
-
bioRxiv - Genomics 2021Quote: ... Membranes were washed three times in TBS-T before incubating with secondary antibodies (HRP-anti Mouse-IgG (H+L) (Promega)) for 45 min in TBS-T and 2% milk ...
-
bioRxiv - Cell Biology 2020Quote: ... Membranes were washed 3 times for 15 min in TBST and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10000; Promega) in 3% BSA-TBST for 1h at room temp ...
-
bioRxiv - Cancer Biology 2022Quote: ... The blots were incubated with secondary antibody (horseradish peroxidase-conjugated goat anti-mouse (Cat# W402B) or goat anti-rabbit IgG (W401B) from Promega, Madison ...
-
bioRxiv - Microbiology 2020Quote: ... the plates were washed with PBS-T five times and added with a 1:5000 dilution of HRP-conjugated goat anti-mouse IgG antibody (Promega) in blocking buffer at 37 °C for 45 min ...
-
bioRxiv - Microbiology 2020Quote: ... The plates were washed with PBS-T 5 times and a 1:5000 dilution of HRP-conjugated goat anti-rabbit IgG antibody (Promega) was added for 45 min at 37 □ ...
-
bioRxiv - Genetics 2021Quote: After the FISH protocol was complete gonads in PBS were incubated with primary antibodies diluted in PBS and 0.5 units/µl of RNasin (Promega N261A) at 4°C overnight ...
-
bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... before re-suspending in 50 μl flow buffer containing anti-Nluc polyclonal primary antibody raised in rabbit (Kindly gifted by Promega) at 1:100 dilution and incubated at room temperature for 1 hour ...
-
bioRxiv - Biochemistry 2020Quote: ... diluted in blocking buffer (1:10,000) was used as a secondary antibody and immunogenic bands were developed by the addition of BCIP/NBT substrate (Promega). The reaction was stopped with distilled water.
-
bioRxiv - Microbiology 2021Quote: ... Membranes were washed in 1X PBST and then incubated with the secondary antibody (anti-rabbit IgG conjugated with horse radish peroxidase at 1:10000, Promega). After washing the excess secondary antibody ...
-
bioRxiv - Cell Biology 2020Quote: ... using one of the following antibodies against (all dilutions in PBS): HaloTag® polyclonal anti-rabbit (G928A, Promega, 1:100); ACTN2 monoclonal anti-mouse (EA-53 Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... Blots were then incubated in species specific secondary HRP-conjugated antibody (anti-rabbit, Promega, W4011 and anti-mouse, Promega, W4021) for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... Blots were then incubated in species specific secondary HRP-conjugated antibody (anti-rabbit, Promega, W4011 and anti-mouse, Promega, W4021) for 1 hour at room temperature ...