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Citations for Promega :
651 - 700 of 1455 citations for Nipah Virus Glycoprotein G Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested with endoproteinase LysC (Wako Chemicals) and trypsin (Promega) overnight at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described [88] ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested by addition of 1 µg of Trypsin (Promega) and incubated overnight at 37 °C at 600 rpm on a thermoshaker ...
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bioRxiv - Microbiology 2023Quote: ... and the protein solution was digested with 0.5 µg trypsin (Promega) overnight at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... 300 mM NaCl and protein inhibitor cocktail (Promega, Madison, Wisconsin, USA) at pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... After 24h the cells were harvested using protein lysis buffer (Promega) and centrifuged for 10min at 13,000rpm ...
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bioRxiv - Cell Biology 2023Quote: Proteins were cleaved with the trypsin/endoproteinase LysC mixture (Promega, V5072) using the paramagnetic bead-based method (54) ...
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bioRxiv - Biochemistry 2023Quote: ... then digested with lysC/trypsin mixture (1: 50, enzyme: protein, Promega) at 37℃ overnight ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ug of protein per sample was digested with trypsin (Promega) overnight at 37°C using S-TRAP micro cartridges (Protifi LLC,) ...
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bioRxiv - Synthetic Biology 2023Quote: ... His-tag proteins have been purified by using MagneHis system (Promega) and DynaMag spin magnet (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... We added Nano-Glo Luciferase Assay Substrate and LgBiT protein (Promega) and imaged using the ImageQuant LAS4000 Imager.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were in-gel digested using 1µg trypsin (Trypsin Gold, Promega)(37) ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were digested in-gel using modified trypsin (sequencing grade, Promega), as previously described 58 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted from beads using partial on-bead trypsination (Promega) followed by a second digestion ...
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bioRxiv - Genetics 2024Quote: ... Proteins were immediately solubilized in 8 M urea and tryptic (Promega) digestion was performed overnight at 37°C with shaking followed by desalting ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were digested on beads with 1 μg trypsin (Promega, UK) for 18 hours at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Protein digestion was carried out with sequencing grade modified trypsin (Promega) at 1/50 protease/protein (wt/wt ...
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bioRxiv - Immunology 2023Quote: Proteins were digested with sequencing-grade trypsin (Promega, Fitchburg, MA, USA). Each sample was analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... the purified protein was incubated with 50 µM Halo-Alexa488 (Promega) for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Equal amounts of RNA (1 μg) were reverse-transcribed with a mixture of oligo(dT)15 and random hexamer primers using M-MLV polymerase (Promega). RT-qPCR gene expression quantifications were performed using AceQ qPCR SYBR Green Mix (Vazyme Biotech ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg of each env clone was co-transfected with 4 μg of pNL4.3-ΔEnv-vpr+-luc+ into 293T cells in a T25 flask using the FuGENE6 transfection reagent (Promega, USA). The culture supernatants containing the pseudoviruses were harvested at 72 hours post transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.25 µg of DNA was used to amplify specific regions of Trp53 and Nf1 gene using Go-Taq DNA Polymerase (Promega). The primers sequences are summarized in Suppl ...
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bioRxiv - Bioengineering 2020Quote: VSV-G-pseudotyped lentiviral vectors were produced by co-transfecting HEK293T cells with lentiviral backbone plasmids and packaging plasmids (pHDM-Hgpm2, pHDM-tat1b, pRC/CMV-rev1b, pHDM-G) using Fugene HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: The medium in 96-well plate was replaced at the indicated time points with 100 μl of PBS supplemented with 0.2 g/l EDTA and 50 μM FITC-VAD-fmk (Promega, USA) or 50 μM 1,1’,3,3,3’,3’-hexamethylindodicarbo-cyanine iodide (DiIC1(5) ...
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bioRxiv - Molecular Biology 2022Quote: ... were co-transfected with psPAX2 and pCMV-VSV-G packaging plasmids into HEK293T cells using FuGENE 6 (Promega, WI, USA). Supernatants containing virus were collected at 48h and 72h after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... The total RNA (1-2 μg) was reverse transcribed with a GoScript™ Reverse Transcriptase cDNA reverse transcription kit according to the manufacturer’s instructions (Promega Corporation ...
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bioRxiv - Plant Biology 2022Quote: ... and 5 μg plasmid DNA was co-transfected with same amount of low-rate constitutively expressed pRL-TK Renilla luciferase vector (Promega) into mesophyll protoplasts isolated from A ...
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bioRxiv - Molecular Biology 2022Quote: ... Retroviral supernatants were obtained from 293GP cells co-transfected with pVSV-G and pBABE-puro-hTERT or pBABE-hygro-KRAS-V12 constructs (kind gifts from Jerry Shay) using FuGENE HD (Promega) and passed through a 0.45 um filter ...
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bioRxiv - Molecular Biology 2021Quote: The 1-450bp DNA region of the luciferase coding sequence was synthesized with G/C content optimized and ‘ATG’ codons removed and then inserted into the pGL3-Promoter vector (E1761, Promega) in place of the original full-length luciferase coding region using ClonExpress®II One Step Cloning Kit (C112-01 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μg of DNase-treated total RNA was applied for cDNA synthesis using an Improm-II Reverse transcription system (A3802, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... were transfected at 80-90% confluence with lentivirus helper plasmids pVSV-G and psPAX2 in along with the constructed lentiviral transfer vector using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and a non-target control (NT-Ctrl) along with the packaging plasmids (pHCMV-G, and pHCMV-HIV-1) (44) using FUGENE-HD transfection reagent (Promega). The mouse Drp1-targeted shRNA plasmid with the sense sequence of GGCAATTGAGCTAGCTATA ...
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bioRxiv - Microbiology 2023Quote: ... 5 mM MgCl2, 1.5 mM KCl, 100 μ g/ml cycloheximide, 1mM DTT, 200 U/ml RNase in from Promega, 0.5% Sodiumdeoxycholate ...
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bioRxiv - Cancer Biology 2022Quote: The cell viability was measured by MTS assay according to the manufacturer’s protocol (Cat. No. G-111, Promega, Madison, WI) and as described previously16,26 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted (2000 g x 5 min) and subjected to genomic DNA (gDNA) extraction using the Wizard Genomic DNA Purification kit (Promega), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... and the lentiviral packaging plasmids pVSV-G, pDVPR, and pAdvant (gifts from Jan Carette, Stanford University) using FuGENE HD transfection reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 1.5 µg of the pAC8RedNK vector and 0.5 µg of linearized baculoviral DNA were concurrently transfected into 1×106 Sf9 cells using 6 µl of FuGene HD (Promega) in ESF 921 Insect Cell Culture medium (Expression Systems) ...
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bioRxiv - Molecular Biology 2023Quote: ... together with pCG-gag-pol and pCG-VSV-G (a gift from Dr. T. Yasui, National Institutes of Biomedical Innovation, Health and Nutrition) using FuGENE HD (E2311; Promega) for 4 h in Opti-MEM (31985-070 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with packaging vectors pMD2.G and psPAX2 along with relevant shRNA using Fugene 6 transfection reagent (Promega). Viral particles were harvested after 48 hours in DEMEM/ 40% FBS ...
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bioRxiv - Bioengineering 2023Quote: ... A pantropic VSV-G pseudotyped lentivirus was produced via transfection of Lenti-X 293T cells with the pHR transgene expression vector and viral packaging plasmids pCMVdR8.91 and pMD2.G using Fugene HD (Promega #E2312). At 48 hours ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus was generated in HEK293T cells by transfecting lentiviral transfer vector, and helper vectors (psPAX2, and pVSV-G) using Fugene HD (Promega). Lentiviral supernatant was collected 48 hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were then plated onto 35 mm glass coverslips pre-coated with poly-D-lysine (100 g/mL) and transfected with 300 ng of each plasmid using FuGene transfection reagent (Promega) or Lipofectamine 2000 (ThermoFisher) ...
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bioRxiv - Plant Biology 2019Quote: ... An aliquot containing 4 μg RNA was treated for 45 min with 2 μl of DNase RQ1 (1 μg/μl) at 37 °C (Promega, UK), and purified using an RNAeasy spin column (Qiagen ...
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bioRxiv - Neuroscience 2019Quote: ... The LCLs were further screened for karyotype abnormalities using G- banding approach and sample identity confirmation was done by STR profiling [GenePrint® 10 System (Promega)].
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bioRxiv - Cell Biology 2021Quote: ... psPAX2 and pMD2.G at the ratio of 1:1:1 in HEK293T cells using ProFection Mammalian Transfection System (Promega, E1200), medium was changed 16h post transfection and virus containing supernatant was harvested 48h later ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were reverse-transfected at the day of seeding with 17µg of plasmid either coding for eGFP-RNF213 or eGFP-RNF213-ΔC using 51 µL of Fugene® HD (#E2311, Promega) for a ratio of 1:3 (µg cDNA / µL transfection reagent) ...
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bioRxiv - Cell Biology 2021Quote: ... was synthesized from 1 μg of total RNA, using oligo-dT primers (USB Corporation, Cleveland, USA) and a reverse transcriptase kit (ImPROm-II, Promega, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: 5’-LTR of HERV-R was cloned in fusion with the downstream Gaussia Luciferase (G-Luc) into the pGL3 basic vector (Promega, USA). The sequence corresponding to 596 nucleotides of HERV-R 5’-LTR (22061-22657 base pairs on human chromosome 7q11 ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg linearized vector was used for in vitro transcription using the T7 RiboMAX™Express Large-Scale RNA Production System (Promega) according to the manual ...
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bioRxiv - Synthetic Biology 2023Quote: ... with a pLV-EF1a-IRES or pTwist-SFFV transgene expression vector and the viral packaging plasmids psPAX2 and pMD2.G using Fugene HD (Promega #E2312). Viral production was performed in 6-well tissue culture treated plates (Corning 3335) ...