Labshake search
Citations for Promega :
651 - 700 of 1480 citations for Human KRT12 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and target (400 ng) plasmids using 6 μL FuGene HD (Promega, Cat# E2311). 72 hrs later ...
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bioRxiv - Microbiology 2022Quote: ... The extracted plasmid was linearized with restriction enzyme BamHI (Promega, Madison, WI, USA), and thereafter purified with QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... DNA was purified using the Pure Yield Plasmid Midi Prep System (Promega, A2495).
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bioRxiv - Evolutionary Biology 2022Quote: Plasmid and B2 insert were ligated together using a rapid ligation kit (Promega). Ligation reactions contained 2.5µl 4x buffer ...
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bioRxiv - Microbiology 2022Quote: ... Replicon RNA was transcribed from linearized template plasmids with T7 RiboMAX Express (Promega) and cleaned by MEGAclear (Ambion/ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... purified using the PureYield Plasmid Miniprep or Midiprep Systems (Promega # A1222 and A2495), and validated by Sanger sequencing at The Ohio State University Comprehensive Cancer Center Genomics Shared Resource (OSUCCC GSR) ...
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bioRxiv - Biophysics 2022Quote: ... transient transfection of the appropriate plasmid(s) was performed using FuGENE HD (Promega) according to the manufacturer’s procedure ...
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bioRxiv - Microbiology 2023Quote: ... The presence of the plasmid was confirmed by colony PCR (GoTaq polymerase, Promega). For construction of ATCC17978Δhcp::aprR ...
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bioRxiv - Biochemistry 2023Quote: ... purified using the PureYield Plasmid Miniprep or Midiprep Systems (Promega # A1222 and A2495), and validated by Sanger sequencing at The Ohio State University Comprehensive Cancer Center Genomics Shared Resource (OSUCCC GSR) ...
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bioRxiv - Genomics 2023Quote: ... The guide and donor plasmids were transfected into cells using Fugene HD (Promega). After seven days ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfections were performed by complexing plasmids with FuGENE® 6 Transfection Reagent (Promega) in OptiMEM media (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... and pDEST26-Renilla plasmid (0.1 μg) using FuGENE® HD (Promega, Cat# E2312). Twenty-four hours after transfection ...
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bioRxiv - Microbiology 2023Quote: ... The pRL-TK Renilla luciferase reporter plasmid was purchased from Promega (Madison, WI). E ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were transfected into HEK293T cells using the ViaFect™ Transfection Reagent (Promega) according to manufacturer instructions 45.
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bioRxiv - Biochemistry 2023Quote: ... DNA used for transfection was purified using the PureYield Plasmid Midiprep System (Promega). After incubation for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid transfections were performed in MDA-T32 cell line using Fugene 6 (Promega). Transfected cells were cultured for over 3 weeks in complete RPMI-1640 medium containing G418 (600 μg/ml ...
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bioRxiv - Biochemistry 2023Quote: Transient transfection of the dual NanoLuc and Halo-Tagged tagged BRD4Tandem plasmid (Promega) was performed as described previously52 ...
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bioRxiv - Biophysics 2023Quote: ... Purified DNA fragments were ligated into the pGEM®t-easy plasmid (Promega) and transformed into DH5α bacteria selected on standard Luria broth (LB ...
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bioRxiv - Genetics 2023Quote: ... or minimal promoter firefly luciferase reporter plasmid pGL4.26 [luc2/miniP/Hygro] (Promega, #E8441). Note ...
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bioRxiv - Microbiology 2023Quote: ... The firefly luciferase expression plasmid pGL3-CMV was purchased from Promega (Madison, WI).
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bioRxiv - Genomics 2023Quote: gBlocks with selected SNP-containing fragments (IDT) were cloned into pGL4.10 plasmid (Promega). For the luciferase reporter assay ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 µg of the specific lentiviral plasmid/construct using FugeneHD (Promega, E2311) in Optimem (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... The next day cells were transfected with plasmid DNA using Viafect (E4982, Promega) at a ratio of 3:1 viafect to DNA ...
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... and envelop plasmid (VSVG) using FuGENE HD transfection reagent (Promega, Madison, WI, USA). The supernatant containing the constructed lentiviruses was collected after 24–48 h of transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... lentiviral packaging vectors into human HEK-293T cells via calciumphosphate transfection (Promega; Madison, WI, USA), according to manufacturer’s directions ...
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bioRxiv - Cancer Biology 2021Quote: The 3’UTR of human PAQR3 was cloned into pmirGLO vector (Promega, Madison, WI, USA), and then validated by sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... Human SMARCB1 was translated in vitro using TNT Quick Coupled Transcription/Translation System (L1170, Promega). 1 µg of pcDNA3.1-FLAG-SMARCB1 was incubated at 30°C for 90 minutes with 20 µM methionine and TNT T7 Quick Master Mix ...
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bioRxiv - Cancer Biology 2020Quote: ... Human cell lines were DNA fingerprinted for provenance using the Power-Plex 1.2 kit (Promega) and confirmed to be the same as the DNA fingerprint library maintained by ATCC ...
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bioRxiv - Bioengineering 2021Quote: The open reading frame for human SOX17 was PCR amplified using GoTaq Master Mix (Promega) from the PB-TRE3G-SOX17 plasmid (Table S5) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Cell Biology 2020Quote: ... Cignal 45-pathway reporter arrays plasmids were transfected with ViaFect™ Transfection Reagent (Promega).
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bioRxiv - Developmental Biology 2021Quote: ... Reporter plasmid (500 ng) and Renilla phRL-TK control vector (50 ng; Promega E2241) were cotransfected into HEK293T cells cultured in DMEM containing 10% FBS using Lipofectamine LTX Reagent with PLUS Reagent (Invitrogen 15338100) ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmid DNA was isolated using Wizard® Plus SV Minipreps purification kit from Promega and used for yeast transformation by electroporation as described in (Benatuil et al. ...
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bioRxiv - Immunology 2022Quote: ... 10 μg pΔ8.91 packaging and 8 μg lentiviral vector plasmid using Fugene (Promega, #E2312) according manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... was previously described (41) and the FFLuc expression plasmid (pGL4.13) was obtained from Promega. The pcDNA3.1(+ ...
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bioRxiv - Microbiology 2019Quote: ... and the pool of transformants underwent plasmid preparation (miniprep) using a commercial kit (Promega). The miniprep was again digested with EcoRV and NotI and the resulting library was introduced into the conjugation helper strain ET12567-pUZ8002 (Hopwood et al. ...
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bioRxiv - Microbiology 2019Quote: HPC229 plasmids were extracted using the Wizard DNA purification kit (Promega, Madison, WI, USA), and analyzed by 0.7% agarose gel electrophoresis and ethidium bromide staining following described procedures [13] ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid pGEM-1 and the TNT SP6 Transcription/Translation System were from Promega. [35S]Met was from PerkinElmer.
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmid DNA was isolated using the Wizard Plus SV Minipreps DNA purification kit (Promega) or the NucleoBond Xtra Midi kit (Macherey-Nagel) ...
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bioRxiv - Genomics 2019Quote: ... INS 832/13 cells were co-transfected with pGL3 promoter and psicheck plasmids (Promega) using lipofectamine 2000 (Thermofisher) ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid was purified using Wizard® Plus SV Minipreps DNA Purification Systems (Promega). After checking the insert in the plasmid using PCR and electrophoresis ...
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bioRxiv - Genetics 2021Quote: ... 1 μg linearized vector was purified with the PureYield Plasmid Miniprep System (Promega, A1223) and used as template for in vitro synthesis of capped mRNA with the Ambion mMessage Machine T3 Transcription Kit (Thermo Fisher ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... HEK293T cells were transiently transfected with a luciferase gene (nano-luc, plasmid pNL2.2, Promega) under the control of the IL-8 promoter [23] ...
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bioRxiv - Bioengineering 2021Quote: ... pSFG.iCasp9.2A.14G2A-CD28-OX40-CD3ζ plasmid was MidiPrepped using the PureYield MidiPrep system (Promega). One day prior to transfection ...
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bioRxiv - Bioengineering 2021Quote: ... VFC-CAR and VFC-Ctrl plasmids were MidiPrepped using the PureYield MidiPrep system (Promega). PCR amplicons were generated from plasmid templates using Q5 Hot Start Polymerase (NEB ...
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bioRxiv - Bioengineering 2021Quote: ... The amount of plasmid was determined using Picogreen DNA assay (#E2670, Promega, Madison, WI) following the manufacturers’ instructions.
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bioRxiv - Bioengineering 2021Quote: ... while the luciferase expression plasmid pGL4.50 was purchased from Promega (cat# E1310, Madison, WI).
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bioRxiv - Neuroscience 2021Quote: ... All described plasmids were transfected into cells using FuGENE® HD Transfection Reagent (Promega) according to the manufacturers protocol ...
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bioRxiv - Biophysics 2020Quote: ... mRNA was synthesized from these plasmids using a RiboMAX SP6 RNA production kit (Promega). In vitro translation of the mRNA was carried out using rabbit reticulocyte lysate (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA of insert-containing colonies was extracted (Wizard Plus SV Minipreps, Promega, France) and Sanger-sequenced in both directions by Eurofins Genomics (Germany) ...