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Citations for Promega :
651 - 700 of 3715 citations for G Protein Signaling Modulator 1 GPSM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and the lentiviral packaging plasmids pVSV-G, pΔVPR, pAdvant (gifts from Dr. Jan Carette, Stanford University) using FuGENE HD transfection reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The constructs (1.2 µg) were cotransfected with vesicular stomatitis virus G (600 ng) and pSPAX2 (800 ng) using FuGENE 6 (Promega). The lentiviral supernatants were collected 2 days after transfection ...
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bioRxiv - Biochemistry 2020Quote: ... Lentivirus was produced by transfecting HEK293T cells at 80% confluence with a mixture of the transfer plasmid pLX302-Igk-FLAG-Subtiligase-PDGF TM and the second-generation lentiviral packaging plasmids (pMD2.g and pCMV-R8.91) using FuGENE HD transfection reagent (Promega). After 6 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Release of LDH was assessed by LDH CytoTox 96® Non-Radioactive Cytotoxicity Assay (G 1780, Promega, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... the lentivirus was produced by transfecting 70% confluent HEK 293T cells with the desired plasmid along with psPAX2 and pMD2.G following the manufacturer’s instructions (FuGENE HD, Promega). The virus-containing medium was collected 48 h after transfection ...
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bioRxiv - Immunology 2022Quote: ... the lentivirus was produced by transfecting 70% confluent HEK 293T cells with the desired plasmid along with psPAX2 and pMD2.G following the manufacturer’s instructions (FuGENE HD, Promega). The virus-containing medium was collected 48 h after transfection ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Denaturated RNA (5μg) was retrotranscribed and used for quantitative PCR using GoTaq qPCR Master Mix according to the manufacturer’s instructions (Promega) at a dilution of 1/10 for mycelium and 1/7 for rice leaves ...
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bioRxiv - Cell Biology 2022Quote: ... Transfection of the different plasmids was made in a ratio 4:1:4 using lentiviral components pCMVR8,74 (Gag-Pol-Hiv1) and pMDG2 (VSV-G) vectors and the plasmid of interest respectively using FuGENE HD (Promega) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... at 10,000 x g and 4°C for 2 min and digested by addition of RNAse-free DNAse I (Promega) for 10 min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA (1-2 μg) was reverse transcribed with a GoScriptTM Reverse Transcriptase cDNA reverse transcription kit according to the manufacturer’s instructions (Promega Corporation ...
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bioRxiv - Cell Biology 2024Quote: 1μg of each plasmid or pcDNA3.1 empty vector was reverse transfected into 1x106 HEK293T cells using FUGENE HD (Promega) as per manufactures instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... we used a different cAMP-dependent firefly luciferase suitable for Gαi signaling (GloSensor-22F; Promega; E2301) 77 ...
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bioRxiv - Cell Biology 2022Quote: ... blots with α-PRDX6 and α-PfNAPL were incubated with respective α-mouse and α-rabbit horse radish peroxidase (HRP)-coupled secondary antibodies (1:5000, Promega) for 1h at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibody for 30min (anti-mouse IgG HRP conjugate Promega #W402B, anti-rabbit IgG HRP conjugate Promega #W4011, 1:3000) and chemiluminescence was detected using Western Bright ECL spray (Advansta ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies were horseradish peroxidase-conjugated anti-mouse antibodies (cat. no. W402B; Promega, Madison, WI, USA) or rabbit IgG (cat ...
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bioRxiv - Plant Biology 2023Quote: ... bound antibodies were revealed with a peroxidase-linked secondary anti-rabbit antibody (Promega, Madison, WI, USA) and visualized by chemiluminescence (ImmunoStar Zeta ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins in the excised band were digested with Trypsin (Promega, V5280). The resulting peptides were analyzed online by nano-flow HPLC-nanoelectrospray ionization using a Qexactive HFX mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: TNT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) was used to perform in vitro translation assay ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were digested with 10ng/ul of sequencing grade trypsin (Promega) overnight at 37C ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then digested using modified trypsin (Promega, P/N V5113) at a 1:20 (w/w ...
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bioRxiv - Microbiology 2021Quote: ... Crosslinked proteins were digested by the addition of 1μg trypsin (Promega) and overnight incubation at 30°C ...
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bioRxiv - Neuroscience 2020Quote: ... The protein digestion was performed by adding 0.2 µg LysC (Promega) in 50 µL of 100 mM ABC for 3h at 1200 rpm at 37°C followed by overnight trypsin digestion using 0.2 µg of trypsin (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... Fusion proteins were expressed by TnT® Quick Coupled system (Promega). 15 μL of HA tagged protein was then incubated with candidate GST-coated beads at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Protein digestion was carried out with sequencing grade modified Trypsin (Promega) at 1/50 protease/protein (wt/wt ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were digested by addition of sequencing-grade trypsin (Promega) at an enzyme-to-substrate ratio of 1:25 ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were digested with 500 ng trypsin (Promega, Madison, USA) overnight at 37°C on a shaking incubator ...
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bioRxiv - Plant Biology 2021Quote: ... Expressed proteins were directly captured using Magne Halo Tag Beads (Promega). The protein-bound beads were incubated with adaptor-ligated gDNA fragments ...
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bioRxiv - Cell Biology 2021Quote: ... Then the protein was digested with sequencing grade modified trypsin (Promega) (1 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were then digested with 12.5 ng/uL modified trypsin (Promega) in 50 μL 100 mM ammonium bicarbonate ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described (Casabona et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested with Trypsin/ Lys-C (Promega, WI, USA). According to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... Proteins were digested with mass spec grade Trypsin/LysC mix (Promega) at a 1:50 (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples were mixed with Trypsin Gold (Promega, Madison, WI, USA) at a 50:1 ratio (w/w ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested with trypsin overnight (0.12 μg Trypsin Gold [Promega UK Ltd ...
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bioRxiv - Plant Biology 2021Quote: ... Enzymatic digestion of proteins using LysC/Trypsin Mix (Promega, Fitchburg, WI) was then performed according to the technical manual ...
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bioRxiv - Physiology 2021Quote: ... and overnight protein digestion using 4 μg MS-grade trypsin (Promega) at 37°C ...
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bioRxiv - Biochemistry 2020Quote: The protein samples were digested with trypsin (Promega, mass spectrometry grade) in (NH4)HCO3 buffer (0.1 M ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were then digested with 12.5 ng/µL modified trypsin (Promega) in 50 µL 100 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2022Quote: Bead-bound protein was digested with 100 ng trypsin (Promega, V5113) overnight at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested with sequencing-grade modified trypsin (Promega, Madison, WI) overnight at 37 ºC ...
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bioRxiv - Microbiology 2019Quote: ... Other samples were processed using protein precipitation solution (A795A; Promega, France) [12] ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then in-gel digested using trypsin (sequencing grade, Promega). Resulting peptides were analyzed by online nanoLC-MS/MS (Ultimate 3000 RSLCnano and Q-Exactive Plus or HF ...
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bioRxiv - Systems Biology 2019Quote: ... protein was digested in 1.65 µg of sequencing-grade trypsin (Promega) for 6 hours at 37°C ...
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bioRxiv - Genetics 2020Quote: ... 40 μg of extracted proteins were fragmented by trypsin digestion (Promega Sequencing Grade Modified Trypsin V5111) ...
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bioRxiv - Molecular Biology 2020Quote: ... Halo-tag containing proteins were purified using Magnetic HaloLink Resin (Promega) at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37°C with 1µg trypsin (Promega). Peptides were desalted on C18 StageTips ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were then subjected to trypsin digestion (Promega; Madison, WI, USA) at trypsin:protein ratio of 1:50 at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified protein was then digested into peptides using Trypsin (Promega, V5111) and desalted using Pierce C18 Spin Columns (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... and then the proteins were digested to peptides using trypsin (Promega) at a trypsin/protein ratio of 1:50 (w/w ...
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bioRxiv - Cancer Biology 2022Quote: ... PLK1 proteins isolated by gel electrophoresis were digested with trypsin (Promega) in 100 mM NH4HCO3 pH 8.0 ...