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Citations for Promega :
601 - 650 of 1232 citations for Rabbit Anti ALB Recombinant Antibody Clone CA645 ; scFv Fragment since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Ligation of the digested pGEM-T easy vector and 5’ fragments was carried out overnight at 4 °C using 0.5 µl (1.5 U) of T4 DNA Ligase (Promega). pGEM-T easy vector containing the 5’ fragment (5’-pGEM-T ...
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bioRxiv - Microbiology 2022Quote: ... The resulting fragments were ligated to Nextflex adapters (Bio Scientific) with the use of T4 DNA ligase (Promega). The libraries were amplified using an AT-rich optimized KAPA protocol using KAPA HiFi HotStart ready mix (KAPA Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... mutations were generated in a fragment of the L ORF subcloned into a pGEM T easy vector (Promega). The mutated ORF fragment was sequenced and then substituted in the L ORF of pTM1 ...
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bioRxiv - Genetics 2024Quote: ... and a 1500 bp fragment surrounding PI3KR1 transcription start site were inserted into pGL3 Basic vector (Promega, USA) (Supplementary Table 23) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The resulting β-arrestin2 fragment was subcloned in the pBiT1.1-N[TK/LgBiT] vector (kindly provided by Promega) using the indicated restrictions enzymes ...
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bioRxiv - Molecular Biology 2024Quote: ... we employed selected fragments of alternatively spliced exons that were cloned into the pGEM®-T Easy (Promega) Vector (listed in 21) ...
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bioRxiv - Microbiology 2020Quote: ... Clone libraries were generated with the pGEM®-T Easy Vector system (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The expression vectors of BRD4-Halo and p300-Halo were Kazusa clones (Promega). The expression vector of SF2(SRSF1/ASF)-mRFP has been described previously (Yomoda et al. ...
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bioRxiv - Biophysics 2022Quote: ... pHalo-PR open reading frame (ORF) clone was purchased from Promega (Promega #FHC24423). pHalo-ER expresses human ERα fused to HaloTag in the C-terminus regulated by a CMVd1 promoter and has been described previously (21 ...
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bioRxiv - Cancer Biology 2023Quote: ... Final clones or pools were authenticated using the PowerPlex® Fusion System (Promega) performed at the Hartwell Center for Biotechnology at St ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA from the selected clone was purified using the EndoFree Plasmid kit (Promega) and sent to the TaRGet vector core to generate the corresponding AAV-RLBP1 vector (production titres 1.2×1012 vg/ml (pDDO batch in DPBS 1X without CaCl2 and MgCl2 ...
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bioRxiv - Microbiology 2024Quote: ... Clones were screened by colony PCR (cPCR) with 2x GoTaq Green Mastermix (Promega) and plasmids were isolated with a QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were dissolved in 50 μL of 50mM TEAB and treated with 1 μL of recombinant PNGaseF (Promega, Madison, WI) overnight at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from pooled neurons that were stored at −80 in saline solution with 1% (v/v) Recombinant RNasin Ribonuclease Inhibitor (Promega). The RNA was extracted with the Quick-RNA micro prep kit (Zymo ...
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bioRxiv - Cancer Biology 2021Quote: ... 293T cells were transfected with 8xTEAD synthetic YAP/TAZ-responsive promoter-luciferase reporter and treated with BSA or recombinant TNC (5 µg/mL) for 48 h to measure YAP1 signaling activity using Dual-luciferase Reporter Assay Kit (Promega).
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bioRxiv - Cancer Biology 2022Quote: ... The N-glycans were then released using 10 U recombinant Elizabethkingia miricola N-glycosidase F (Promega, V4831, 10 U/μL) for 16 h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... anti-m6 A conjugated beads were incubated with purified mRNA with rotation at 4C overnight in 300 mL MeRIP buffer with 1 mL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 100 ng of either XeARPP19 or ClyARPP19 were incubated with 200 μM ATP and 25 units of recombinant bovine PKA (Promega) in PKA Buffer for 3 hours at 37°C under 1200 RPM stirring ...
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bioRxiv - Biochemistry 2023Quote: ... then alkylated (100 mM iodoacetamide in 100 mM ammonium bicarbonate at room temperature for 45 minutes) prior to in-gel trypsin digestion (30 μL of 6 ng/μL recombinant sequencing-grade trypsin (Promega) in 100 mM ammonium bicarbonate per cubed band ...
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bioRxiv - Neuroscience 2023Quote: Nine DIV hippocampal slice cultures were randomly assigned to treatment groups with the following drugs dissolved in serum-free medium: recombinant human BDNF (250 ng/mL, Promega); BDNF + K-252a (200 nM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Initial P0 virus was obtained by addition of ∼3 µg recombinant bacmid DNA mixed with 3 µl FuGENE HD Transfection reagent (Promega) in 100 µl Sf900 II media (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant methylated BCAR3 (see methylation assay method) was sliced from the gel and digested with modified trypsin (sequencing grade, Promega). The resulting peptides were analyzed by online nano liquid chromatography (LC)-MS/MS (UltiMate 3000 RSLCnano and Q-Exactive HF ...
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bioRxiv - Cell Biology 2022Quote: ... 60,000 nuclei were sorted using an SH800 sorter (Sony) into 50 μl of collection buffer [1.0U/μL Recombinant RNAsin (Promega, PAN2515), 5% Fatty-acid-free BSA in PBS (Proliant ...
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant GST or GST-YgfB protein at a concentration of 10 μM was incubated with 100 μl 50 % MagneGST (Promega) bead-slurry equilibrated with pulldown-buffer for 45 min at 4°C and washed two times with 500 μl pulldown-buffer ...
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bioRxiv - Developmental Biology 2024Quote: Substrate titration kinase reactions were performed on the indicated recombinant LIN-42 proteins (CK1BD + Tail WT or mutants) using the ADP-Glo kinase assay kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant pNZ8037 plasmid carrying wild-type or mutated ptnCD genes was isolated using PureYieldTM Plasmid Mini-prep System (Promega, USA). Targeted changes in the ptnCD genes were introduced following the Site Directed Mutagenesis Cornell iGEM 2012 Protocol (http://2012.igem.org/wiki/images/a/a5/Site_Directed_Mutagenesis.pdf) ...
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bioRxiv - Biochemistry 2024Quote: Kinase reactions were performed on the recombinant human PER2 FASP or Degron using the ADP-Glo kinase assay kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: The Rabbit Reticulocyte Lysate System (Promega) was used to determine the influence of internal modifications on translation efficiency of RNA F ...
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bioRxiv - Biophysics 2023Quote: Rabbit reticulate lysate (RRL, L4960, Promega) is used as the artificial cytoplasm to fulfill the requirement of cytosolic factors necessary for nuclear transport (S ...
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bioRxiv - Cancer Biology 2024Quote: Rabbit reticulocyte lysates (RRL) (Promega L4960) were treated with micrococcal nuclease (NEB M0247S ...
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bioRxiv - Cell Biology 2024Quote: ... Rabbit reticulocyte lysate (Promega, Madison, WI) was diluted 1:1 by the addition of cold mix buffer (100 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then incubated for 1h at room temperature with horseradish peroxidase-conjugated anti-mouse IgG secondary antibody (Promega) at 1:10,000 and peroxidase was detected using the Pierce chemiluminescence system (Pierce ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was a horseradish peroxidase polyclonal goat anti-mouse (from 1:5,000 to 1:10,000, depending on the primary antibody; Promega, W4021). Proteins were detected with the ECL chemiluminescence reagent (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... This was done by cloning the 93-bp MEP amplified fragment obtained above into pGEM T-easy vector (Promega) and transforming into Escherichia coli XL-1 blue ...
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bioRxiv - Cell Biology 2020Quote: ... the artificially synthesized PlGF 3’ untranslated regions (UTR) gene fragment was constructed into pMIR-reporter (Promega, Madison, WI, USA). A complementary sequence with mutation of the seed sequence was designed based on the wild type (WT ...
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bioRxiv - Developmental Biology 2021Quote: ... synthesized as gBlock fragments (IDT) and were cloned into the Eco RV site of pGL4.23-luc2/minP vector (Promega) using Gibson Assembly ...
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bioRxiv - Genomics 2021Quote: ... Variant or variant haplotype fragments were amplified in African American heterozygous individuals carrying the variants of interest located in the middle of the fragments (Supplementary Table 2 and 3) and cloned into the enhancer reporter vector pGL4.24 (E8421, Promega). Subsequently ...
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bioRxiv - Plant Biology 2020Quote: ... Absolute quantification was obtained with DNA fragment from the Map gene cloned in pGEM®-T Easy vector (Promega) with 10 to 108 copies of the plasmid added on each PCR plate ...
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bioRxiv - Molecular Biology 2020Quote: Prg4 enhancer/promoter-firefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector (Promega). Bovine genomic DNA was extracted from bovine chondrocytes and was used as the template to amplify Prg4 regulatory regions ...
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bioRxiv - Neuroscience 2021Quote: ... in which a 1309-bp genomic DNA fragment of the murine Gpr19 (−1083 to +226)-luciferase reporter (luc2P, Promega) was cloned into a vector engineered to contain the PB IRs and internal sequences necessary for efficient chromosomal integration (Nakanishi et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... selected fragments of alternatively spliced exons (Supplementary Table 7) were cloned into the pGEM®-T Easy Vector (Promega). To generate the DNA templates for transcription ...
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bioRxiv - Microbiology 2021Quote: ... Restricted fragments to be cloned were ligated into the EcoRI and SacI restricted pBAD/His using T4 Ligase (Promega) before being heat shock-transformed into chemically competent E ...
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bioRxiv - Evolutionary Biology 2021Quote: Well-amplified fragments were cloned into a pGEM-T vector using the pGEM-T Easy Vector System (Promega A1360), following the manufacturer’s instructions and bacteria were cultured on LB-agar plates supplemented with ampicillin (150 ug/ml) ...
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bioRxiv - Molecular Biology 2023Quote: The human MEOX1 enhancer syntenic to the mouse Meox1 peak9/10 enhancer3 fragment along with the mouse Il1b peak5 and peak6 fragments were PCR amplified and cloned into the XhoI and HindIII sites in the Firefly luciferase plasmid pGL4.23 (Promega) using the Cold Fusion kit (System Biosciences Inc ...
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bioRxiv - Neuroscience 2023Quote: ... A 9.5 kb fragment containing upstream promoter region of mouse Frizzled10 (Fzd10) gene was subcloned into pGEM vector (Promega). CreERT2gene (Feil et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR fragments with expected size were purified and TA cloned in the pGEM-T Easy Vector (Promega, Madison, WI) for DNA sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... Purification and assembly of DNA fragments were performed using a Wizard SV Gel and PCR Clean-up System (Promega) and a NEBuilder HiFi DNA assembly cloning kit (New England Biolabs) ...
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bioRxiv - Developmental Biology 2023Quote: Digoxigenin-labelled antisense riboprobes were synthesized from cloned cDNA fragments (Supplementary file 1) using T7 or SP6 polymerases (Promega) and digoxigenin-labelled dUTPs (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: A construct bearing 2 kb of the murine Bigheart promoter (pGL3-B.HTSS2KB) was subcloned as a HindIII and SacI fragment into the pGL3-Basic vector (Promega). pGL3-Intron3-DSCR1 harbors the complete (875 bp ...
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bioRxiv - Genetics 2022Quote: ... The obtained DNA fragment containing the gtr1Q86L allele was cloned into the pGEM-T vector (Promega, Madison, WI, USA), verified by Sanger sequencing and used to co- transform protoplasts of the F ...