Labshake search
Citations for Promega :
601 - 650 of 655 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: MTS assays of HCoV-229E experiments were performed using the The CellTiter 96® AQueous One Solution Cell Proliferation Assay kit (Promega, #G3582). In brief ...
-
bioRxiv - Molecular Biology 2020Quote: ... The band was cut and washed before the in-gel digestion of the proteins overnight at 37°C with a solution of modified trypsin (sequence grade, Promega, Charbonnières, France). The resulting peptides were extracted from the gel by one round of incubation (15 min ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, 182 Madison, WI) at 4ºC ...
-
bioRxiv - Cancer Biology 2021Quote: The effect of P2RX7B expression and inhibition on OS cell proliferation was assessed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, UK), TE85 ...
-
bioRxiv - Microbiology 2021Quote: ... Primary human airway epithelial cells were homogenized in homogenization solution supplemented with 1-thioglycerol and lysed by addition of lysis buffer (Maxwell 16 LEV simplyRNA cells kit; Promega #AS 1270). Prior to RNA extraction ...
-
bioRxiv - Microbiology 2020Quote: ... medium was removed from the cells and replaced by MTS reagent (CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega, Madison, WI) diluted in PBS ...
-
bioRxiv - Microbiology 2021Quote: ... was performed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay kit using manufacturer’s instructions from Promega (Madison, WI, USA). Vero E6 cells were seeded in 96-well plates at a density of 10,000 cells/well ...
-
bioRxiv - Neuroscience 2023Quote: ... a subset of cells underwent viability testing via MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega, Catalog #G3582) or ATP assay (CellTiter-Glo® Luminescent Cell Viability Assay ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 24-48hrs in the blocking solution containing chicken anti-human NT-4/5 antibody (10 mg/ ml, Promega, Madison, WI) or rabbit anti-human NT-4/5 (1:1000 ...
-
bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
-
bioRxiv - Microbiology 2023Quote: ... Sample digestion was carried out by resuspending beads in 1 µL of trypsin solution (0.2 µg trypsin in Promega V5111 resuspension buffer) and 19 µL 50 mM ammonium bicarbonate and incubating at 37 °C with agitation for 18 hours ...
-
bioRxiv - Bioengineering 2023Quote: ... the liquid DMEM culture medium and the hydrogel overlaying the cultured chondrocytes were first removed before introducing 300 µL of CellTiter 96® Aqueous One Solution Cell Proliferation assay (Promega, Australia) working solution ...
-
bioRxiv - Cancer Biology 2023Quote: ... The beads were washed using four cycles of resuspension and pulse centrifugation in Dynabeads wash solutions containing 0.05% Tween 20 (H5152, Promega, Madison, WI, USA), followed by three washes in 1X PBS (10010072 ...
-
bioRxiv - Developmental Biology 2022Quote: ... protein in the gel pieces was digested using 20 ng/μl of Trypsin solution made in 25mM ammonium bicarbonate (Promega, Madison, WI) by incubating at 37°C overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... Control and treated cells were cultured for 1 day in 96-well plates then treated with small molecules for 3 consecutive days before incubation with 120 μL fresh media containing 20 μL of CellTiter 96® AQueous One Solution (Promega, G3580) for 1 to 4 hours at 37°C in a humidified ...
-
bioRxiv - Cancer Biology 2023Quote: ... The protein solution was spun onto an S-Trap device (Protifi, Farmingdale, NY) and digested by 750 ng trypsin (Promega, Madison, WI) in 100 mM TEAB buffer at 37°C overnight ...
-
bioRxiv - Developmental Biology 2023Quote: ... Slides were incubated in freshly made stain solution (5mM potassium ferricyanide + 5mM potassium ferrocyanide + 1mg/mL X-gal (Promega cat no. V3941) in wash buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were cultured for 1 day in 96-well plates and treated with small molecules for 3 consecutive days prior to incubation with 120 μL fresh media containing 20 μL of CellTiter 96® AQueous One Solution (Promega, G3580) for 1 to 4 hours at 37°C in a humidified ...
-
bioRxiv - Biophysics 2024Quote: ... The samples were dried again and rehydrated with 20 µl of trypsin solution with 0.01% ProteaseMAXTM surfactant comprised of 10 ng µl-1 Trypsin (Promega Corp., Madison, WI) in 25 mM NH3HCO3 / 0.01% w/v of ProteaseMAXTM from Promega Corp ...
-
bioRxiv - Neuroscience 2024Quote: ... Frozen brain tissue was homogenised in a dounce-homogeniser containing 2 ml ice-cold lysis solution (Nuclei EZ Lysis Buffer [Sigma-Aldrich, NUC101] or Nuclei PURE Lysis buffer [Sigma-Aldrich, NUC201] with 1 mM dithiothreitol [DTT, Promega, P1171 ...
-
bioRxiv - Microbiology 2024Quote: THP-1 macrophage viability was determined by colorimetric method using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega; G35C) as per the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: Cell viability of the inks screening assays and the bioprinted samples was assessed using the 96®AQueous One Solution Cell Proliferation assay (MTS assay, Promega, USA). The supplier protocol was used ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured 72 h after the drug treatment using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Spain). PBS-treated cells were used as control ...
-
bioRxiv - Systems Biology 2024Quote: ... plates were incubated for 10 days in 12L:12D conditions and treated with 50 µl of 1:50 of furimazine substrate solution (Promega, Southampton UK): 0.01% Triton X-100 ...
-
bioRxiv - Cancer Biology 2024Quote: Cell growth and viability were assessed using the MTS CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, Wisconsin, US) after 72 hours of treatments ...
-
bioRxiv - Cancer Biology 2024Quote: ... The cell proliferation was analyzed after 48 hours culture using CellTiter One Solution Cell Proliferation Assay kit (MTS assay, Promega, Cat# G8461) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... The A2780 cell proliferation was analyzed 48h after cultured in CM using CellTiter One Solution Cell Proliferation Assay kit (MTS assay, Promega, Cat# G8461) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... the membranes were developed with a commercial solution of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT) according to the manufacturer (Promega, Madison, WI), in alkaline developing solution ...
-
bioRxiv - Cell Biology 2022Quote: Proliferation assays were performed in 96 well plates (2 × 103 NMCCs/well) and assayed at 48 h using the CellTiter96® AQueous One Solution Cell Proliferation Assay kit (MTS; Promega, Madison, WI) (38) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cell numbers were quantified using a colorimetric 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS)-based CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2020Quote: MTS growth assays were performed on adherent cells by plating 1000-5000 cells per well in a 96-well plate and assaying using CellTiter 96® AQueous One Solution (Promega, Madison, WI) and incubating for 2 hours and reading at 490nm ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: The evaluation of the cytotoxicity of NBCoV small molecules in the different cell types was performed in parallel with the antiviral activity assay and measured by using the colorimetric CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI) following the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 96-well plates and then maintained in the presence of vehicle or increasing doses of GSK591 for six days before the addition of 20 μL CellTiter 96 AQueous One Solution per well (Promega Corporation, Madison, WI). Plates were incubated for 2 hours before absorbance at OD490 was measured with a Synergy H4 Hybrid microplate reader (Bio Tek ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell viability at 72-h incubation was measured by MTS cell proliferation colorimetric assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay system, Promega, G3582, Italy). Cell numbers at 0 ...
-
Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: Viability of untreated cells or treated cells for 5 days was assessed using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega®, ref:G3580) according to manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2020Quote: ... Gel pieces were resuspended in 20 μL l of trypsin solution (25 ng/ μL trypsin in 100 mM NH4HCO3) (Promega, Cat. No V5111) and incubated at 4°C for 20 min ...
-
bioRxiv - Immunology 2021Quote: ... The plates were washed three times and the reaction was developed with 100 µL ready-to-use TMB solution (Promega, Madison, WI, USA) at RT for 20 min in the dark ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: The cytotoxicity of NBCoV small molecules in the different cell types was performed in parallel with the neutralization assay and evaluated using the colorimetric CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI) following the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2022Quote: Cytotoxicity assay was performed on HCT116 human colon cells (ATCC, #CCL-247™) using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, #G3580) which is a colorimetric method based on MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium ...
-
bioRxiv - Microbiology 2023Quote: ... and the reaction was revealed with a solution of NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate) (Promega, Madison, WI, USA).
-
bioRxiv - Microbiology 2024Quote: ... the viability of HAEs was assessed by a MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay; Promega, Madison, WI, USA) by addition of 20 µl of MTS solution with 180 µl of Opti-MEM on the apical pole of HAEs ...
-
bioRxiv - Molecular Biology 2024Quote: Metabolic activity of CpdG or DMSO treated and oexORP7 or oexMYC cells was measured using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega cat #: G358C), according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2024Quote: ... 25 µg of phloem sap and 50 µg of leaf tissue protein extract were then digested in solution using a Trypsin/Lys-C mixture (Mass Spec Grade, Promega, Madison, WI, USA) according to the instruction manual ...
-
bioRxiv - Immunology 2024Quote: Cytokine or chemical inhibitor doses were selected based on available EC50 data and toxicity profiles with untreated macrophages (Trypan Blue staining or MTS assay; CellTiter 96 Aqueous One Solution Cell Proliferation Assay Promega, Madison, WI, USA). Only inhibitor doses resulting in >90% cell viability were utilized ...
-
bioRxiv - Cell Biology 2024Quote: ... On-pellet in-solution protein digestion was performed in 100 µL 50 mM ABC (pH 8) by adding Trypsin/LysC (Promega, 1:50 ratio) to precipitated ASC proteins ...
-
bioRxiv - Microbiology 2021Quote: ... Crystal violet was the solubilized with a 75% ethanol solution and the absorbance was measured at a λ600nm with a plate reader (Promega GloMax Explorer, Promega, France).
-
bioRxiv - Cancer Biology 2020Quote: ... BXPC-3 and CAPAN-1 pancreatic cancer cells treated with UR214-9 was measured using the Cell Titre96R Aqueous One Solution Cell Proliferation Assay (Promega Corp., catalog number: G3580) following the procedure published earlier ...
-
bioRxiv - Microbiology 2021Quote: ... and samples were incubated at room temperature for two hours before stopping the reaction with 30 μL of the DNase stop solution (Promega Corp. Madison, WI, USA). The eight DNase-treated samples underwent DNA extraction at this point ...
-
bioRxiv - Molecular Biology 2024Quote: The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfo-phenyl)-2H-tetrazolium (MTS) assay (CellTiter 96® Aqueous One Solution Cell Proliferation Assay, Promega, Madison, WI, USA). Ramos cells were seeded in 96-well plates at density of 105 cells/well and incubated for 48 h prior to experimental treatments ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Bacterial cells were lysed by adding 500 μl of Nuclei lysis solution (Component of Wizard®, Wizard® SV and Wizard® SV 96 Genomic DNA Purification Systems, Promega, Madison WI, USA) and incubating at 80°C for 5 min ...